>VHE212 (VHE212Q) /CSM/VH/VHE2-A/VHE212Q.Seq.d/
AACACACATAAAAATGGTAATAAAAAAATTATTTATATTAATTTTTTGTTTGTTTTTAAT
TATCAATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAAAAAATCAAAACCAAA
ACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTG
GTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTGT
TCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCCAGAAAGAAAGTTTAT
TTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAAA
TATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAATGGTGGGTATTGTAT
GAATGATGAGGCTACTACCTACTATGACGATACTATTGACCAAATGACATTGGGCCATCA
ATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGGCTGGCACATAGATCCATTTGG
CCATAGTGCAACACAAGCTCGTATCTTTGGTCAATTAGGATTCGATGCATTCATTATCGG
TCGTATGGAXXXXXXXXXXTAACCAACTATCGTCCATCATGGAATTTAACCGTGGTTCAA
CCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAA
TCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGT
CAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAA
CCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCA
AGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCA
ATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTAC
TCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGG
TTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGT
CAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATT
ACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCA
AGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCATCATCATCAACT
AAAGAAGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAATGCAAATCAGAACTTTTA
AATTACTA

THIKMVIKKLFILIFCLFLIINEINGKKTKINDIKKSKPKLSSTLLNVHIVAHTHDDVGW
LKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLANPERKFIYVEIAFFQRWWDEQSTTMQN
IVKGLVESGQLEFINGGYCMNDEATTYYDDTIDQMTLGHQFLWENFGVMPKIGWHIDPFG
HSATQARIFGQLGFDAFIIGRM---

---TNYRPSWNLTVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDM
MMHRRTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTT
TQQSSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQ
DPQTITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDS
SKVLFLLFLQCKSELLNY

Frame A:
nthkngnkkiiyinflfvfnyq*n*wkknknk*y*kiktktiiniikcsyccsys**cwl
vkns**illwikyvncicrcsiyfgyshylfigksrkkvylc*ncifpkmvg*tkynnak
yc*gfg*kwsigihkwwvlye**gyyll*ryy*pndigpsiplgefrsyakdrlahrsiw
p*cntssylwsirircihyrsyg---

---*ptivhhgi*pwfnqpvvimyl*mllptfkilinhynsplslievvdvhr*emvnwi
**civvl*kmmvvvlvnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclq
plnnlqtigilnikvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkih
kihklsl*ifqlsfqqlqsllqlk*i*lvfknyqiyqd*ngkllmvktmiinhhhqlkki
lqrfcfyffsnanqnf*it

Frame B:
THIKMVIKKLFILIFCLFLIINEINGKKTKINDIKKSKPKLSSTLLNVHIVAHTHDDVGW
LKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLANPERKFIYVEIAFFQRWWDEQSTTMQN
IVKGLVESGQLEFINGGYCMNDEATTYYDDTIDQMTLGHQFLWENFGVMPKIGWHIDPFG
HSATQARIFGQLGFDAFIIGRM---

---nqlssimefnrgstnqw*lctcecyclhsrs*siitihhch**kswmcivkrwsigy
ddassyfer*wswcwstne*vnsnryn**idfp*yfklcsislsscctlfitsitpnvyn
hstifkrlefsisrclltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrft
rstnyhfrsfnylfnnynhfcn*nefnwcskiikfikikmenc*w*kl*s*iiiin*rrf
fkgfvftfspmqirtfkll

Frame C:
ht*kw**knyly*ffvcf*lsmklmekkqk*milknqnqnyhqhy*mfilllilmmmlvg
*kqlmniimdqicqlhlqvfnilwiqplpvywqiqkeslfmlklhfskdggmnkvqqcki
llrvwlkvvnwns*mvgiv*mmrllptmtilltk*hwainsfgriselcqr*agt*ihla
ivqhklvslvn*dsmhslsvvw---

---TNYRPSWNLTVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDM
MMHRRTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTT
TQQSSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQ
DPQTITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDS
SKVLFLLFLQCKSELLNY

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE212 (VHE212Q) /CSM/VH/VHE2-A/VHE212Q.Seq.d/                       2452   0.0
VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1524   0.0
VHH791 (VHH791Q) /CSM/VH/VHH7-D/VHH791Q.Seq.d/                       1524   0.0
VHB156 (VHB156Q) /CSM/VH/VHB1-C/VHB156Q.Seq.d/                       1524   0.0
VHK686 (VHK686Q) /CSM/VH/VHK6-D/VHK686Q.Seq.d/                       1518   0.0
AHJ372 (AHJ372Q) /CSM/AH/AHJ3-C/AHJ372Q.Seq.d/                       1516   0.0
AHE423 (AHE423Q) /CSM/AH/AHE4-A/AHE423Q.Seq.d/                       1513   0.0
AHE874 (AHE874Q) /CSM/AH/AHE8-D/AHE874Q.Seq.d/                       1511   0.0
VHI312 (VHI312Q) /CSM/VH/VHI3-A/VHI312Q.Seq.d/                       1511   0.0
AHE874 (AHE874Q) /CSM/AH/AHE8-D/AHE874Q.Seq.d/                       1511   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1392   0.0    3
CK149800|CK149800.1 CsmgEST00336 Culicoides sonorensis female serum-fed midgut cDNA Library Culicoides sonorensis cDNA, mRNA sequence.   117   2e-23  3
DN814237|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   2e-16  4
DN816506|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   2e-16  4
CX633815|CX633815.1 taj44a03.y3 Hydra EST UCI 5 Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   3e-16  4
DN816232| truncated lysosomal acid alpha-mannosidase [Homo sapiens], mRNA sequence.    52   4e-16  4
DN811862| lysosomal alpha-mannosidase [Cavia porcellus], mRNA sequence.    52   4e-16  4
DT613126|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   4e-16  4
DN815247| lysosomal alpha-mannosidase [Cavia porcellus], mRNA sequence.    52   4e-16  4
CB073433|CB073433.1 taa32h01.y1 Hydra EST -II Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   3e-15  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   392   e-110
M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   391   e-107
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   210   1e-52
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...   209   2e-52
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...   208   3e-52
U05572_1(U05572|pid:none) Human lysosomal alpha-mannosidase (MAN...   208   3e-52
AB209921_1(AB209921|pid:none) Homo sapiens mRNA for mannosidase,...   208   3e-52
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   207   7e-52
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...   207   7e-52
JC2200(JC2200)alpha-mannosidase (EC 3.2.1.24) precursor - human       207   1e-51

 psg: 1.01  gvh: 0.62  alm: 0.17  top: 0.43  tms: 0.07  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 20.0 %: nuclear
	 16.0 %: cytoplasmic
	 12.0 %: mitochondrial
	 12.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall

>> prediction for VHE212 is end

>VHE212F.Seq
AACACACATAAAAATGGTAATAAAAAAATTATTTATATTAATTTTTTGTTTGTTTTTAAT
TATCAATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAAAAAATCAAAACCAAA
ACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTG
GTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTGT
TCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCCAGAAAGAAAGTTTAT
TTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAAA
TATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAATGGTGGGTATTGTAT
GAATGATGAGGCTACTACCTACTATGACGATACTATTGACCAAATGACATTGGGCCATCA
ATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGGCTGGCACATAGATCCATTTGG
CCATAGTGCAACACAAGCTCGTATCTTTGGTCAATTAGGATTCGATGCATTCATTATCGG
TCGTATGGANNNNNNNNNN

>VHE212Z.Seq
NNNNNNNNNNTAACCAACTATCGTCCATCATGGAATTTAACCGTGGTTCAACCAACCAGT
GGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATTACAA
TTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGAT
ATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAAT
GAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCT
CAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACA
ACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTG
ACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAAT
CAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCA
CAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACT
TCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAA
TGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCATCATCATCAACTAAAGAAGAT
TCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAATGCAAATCAGAACTTTTAAATTACTA

>VHE212P.Seq
AACACACATAAAAATGGTAATAAAAAAATTATTTATATTAATTTTTTGTTTGTTTTTAAT
TATCAATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAAAAAATCAAAACCAAA
ACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTG
GTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTGT
TCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCCAGAAAGAAAGTTTAT
TTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAAA
TATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAATGGTGGGTATTGTAT
GAATGATGAGGCTACTACCTACTATGACGATACTATTGACCAAATGACATTGGGCCATCA
ATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGGCTGGCACATAGATCCATTTGG
CCATAGTGCAACACAAGCTCGTATCTTTGGTCAATTAGGATTCGATGCATTCATTATCGG
TCGTATGGA----------TAACCAACTATCGTCCATCATGGAATTTAACCGTGGTTCAA
CCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAA
TCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGT
CAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAA
CCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCA
AGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCA
ATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTAC
TCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGG
TTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGT
CAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATT
ACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCA
AGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCATCATCATCAACT
AAAGAAGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAATGCAAATCAGAACTTTTA
AATTACTA