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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE205
Library VH
(Link to library)
Clone ID VHE205
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16292-1|Contig-U16400-1
Original site URL
Representative seq. ID VHE205P
(Link to Original site)
Representative DNA sequence 
>VHE205 (VHE205Q) /CSM/VH/VHE2-A/VHE205Q.Seq.d/
TTGGCCTACTGGAAACAATATATAAATCAATTAAAATAAACACTCACTCGATAGTGTAAA
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTN
NAAAAAAAAAANNNNCCCCCCCCCNAAAANNTTTTTTNNGGGGGGGGGGGGNNANNTTTN
GGGGGNNNAAAANNNNNNAAAAAAAANTTTTTTTNNGGGNAAANNTTTTTTNNAAAAAAA
AANNTTTTTTTNNGNNAAANNGGGNNNAAANCCCCCNTTTTTTNNAAAAAAANNGGGGGT
TTTNNNNNNCGGGGGGGGNANCCNGGGGNNNAANCCCCCNNNNNNANNNTTTTNCNNNNN
AATTTTTTTTNNNNGGGNNNNANTTTTTTNNNTTNGGGGGGGGNTNNNNGGGGGGGGNAA
NAAAXXXXXXXXXXCATATATAATGGAAAGAGGTATCACATTGAATACACTAACCAAGGA
TTTGGAAATGTTTGTTGAACGTTTCAAACGTGAACATAGAATTGGTCTAATTATTAAGAG
TGAACTTGCTAGCAACACCTACTCAACTCATTTCATTTATTCACTCTTCAAAGAAGAAGG
TAAACATTTATTCGATGTCCGTGAATCTATACTCGGTCATTTACAACAAGGTGGTACTCC
TTCTGCAATTGATCGTATCTTTTCAACTCGTTTAATGAATCATTATTATCAATTCTTGGA
AAACGATCTCAAAGAACATGGTCATCTTCAAATGAATGGTTGTATTGGTTTCATTGATGG
TGGTATTCATTACACTCCAATGCAAGAAATGATCGAAGAAATGTCTGATAAATTTAGACG
TCCTCGTTCCCAATGGTGGATGGATTTGGTTGAAACCTCTCAAAATATCTCTGTTTTCCC
TTTAGATGACCCTTCCTCTACT
sequence update 2002.10.25
Translated Amino Acid sequence 
wptgnni*in*NKHSLDSVKEKKKKKKKKKKKKKKKKXFXKKKXXPPPKXFFXGGGGXXX
GXXXXXKKXFFXGKXFXXKKXFFXXKXGXXPPFFXKKXGVXXXGGGXXGXXPPXXXFXXX
IFFXXXXXFXXXGGXXGGGXK---

---YIMERGITLNTLTKDLEMFVERFKREHRIGLIIKSELASNTYSTHFIYSLFKEEGKH
LFDVRESILGHLQQGGTPSAIDRIFSTRLMNHYYQFLENDLKEHGHLQMNGCIGFIDGGI
HYTPMQEMIEEMSDKFRRPRSQWWMDLVETSQNISVFPLDDPSST
Translated Amino Acid sequence (All Frames) 
Frame A:
laywkqyinqlk*tltr*ckrkkkkkkkkkkkkkkkkxfxxkkxxpppxxxfxggggxxx
ggxkxxkkxffxgxxffxkkxxfxxxxgxkxpxfxkkxggfxxrggxpgxxxpxxxxxxx
nffxxgxxfxxxggxxxggxx---

---hi*wkevsh*ih*priwkcllnvsnvnielv*llrvnllatptqlisfihsskkkvn
iysmsvnlysviynkvvlllqlivsfqlv**iiiinswktisknmvifk*mvvlvslmvv
fitlqckk*skkclinldvlvpnggwiwlkplkislfsl*mtlpl

Frame B:
wptgnni*in*NKHSLDSVKEKKKKKKKKKKKKKKKKXFXKKKXXPPPKXFFXGGGGXXX
GXXXXXKKXFFXGKXFXXKKXFFXXKXGXXPPFFXKKXGVXXXGGGXXGXXPPXXXFXXX
IFFXXXXXFXXXGGXXGGGXK---

---iyngkryhieytnqgfgnvc*tfqt*t*nwsny*e*tc*qhllnsfhlftlqrrr*t
fircp*iytrsfttrwysfcn*sylfnsfnesllsilgkrsqrtwsssnewlywfh*wwy
slhsnarndrrnv**i*tssfpmvdgfg*nlskylcfpfr*pfly

Frame C:
glletiyksikinthsiv*kkkkkkkkkkkkkkkkkxxfxkkkxxppxkxfxxgggxxfx
gxkxxxkkxfxxxxxfxkkkxffxxxxxxxpxfxxkxxgfxxxggxxxgxxpxxxxfxxx
ffxxgxxffxxgggxxggxx---

---YIMERGITLNTLTKDLEMFVERFKREHRIGLIIKSELASNTYSTHFIYSLFKEEGKH
LFDVRESILGHLQQGGTPSAIDRIFSTRLMNHYYQFLENDLKEHGHLQMNGCIGFIDGGI
HYTPMQEMIEEMSDKFRRPRSQWWMDLVETSQNISVFPLDDPSST
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP463 (VHP463Q) /CSM/VH/VHP4-C/VHP463Q.Seq.d/                        967   0.0
VHO650 (VHO650Q) /CSM/VH/VHO6-C/VHO650Q.Seq.d/                        967   0.0
VHN894 (VHN894Q) /CSM/VH/VHN8-D/VHN894Q.Seq.d/                        967   0.0
VHN560 (VHN560Q) /CSM/VH/VHN5-C/VHN560Q.Seq.d/                        967   0.0
VHN457 (VHN457Q) /CSM/VH/VHN4-C/VHN457Q.Seq.d/                        967   0.0
VHJ854 (VHJ854Q) /CSM/VH/VHJ8-C/VHJ854Q.Seq.d/                        967   0.0
VHJ546 (VHJ546Q) /CSM/VH/VHJ5-B/VHJ546Q.Seq.d/                        967   0.0
VHJ475 (VHJ475Q) /CSM/VH/VHJ4-D/VHJ475Q.Seq.d/                        967   0.0
VHJ384 (VHJ384Q) /CSM/VH/VHJ3-D/VHJ384Q.Seq.d/                        967   0.0
VHI828 (VHI828Q) /CSM/VH/VHI8-B/VHI828Q.Seq.d/                        967   0.0
own update 2004. 8. 2
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   967   0.0    2
X94340|X94340.1 D.discoideum mRNA for 6-phosphofructo-1-kinase.   967   0.0    1
X89039|X89039.1 D.discoideum mRNA for 6-phosphofructo-1-kinase.   967   0.0    1
AJ430522|AJ430522.1 Dictyostelium discoideum partial pfk gene for ATP:D-fructose  6-phosphate 1-phosphofructokinase, exon 1.   100   1e-16  1
EJ712224|EJ712224.1 1092956083569 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061001798086 3', genomic survey sequence.    48   0.45   1
EJ705753|EJ705753.1 1092956052585 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061001798086 5', genomic survey sequence.    48   0.45   1
DX414156|DX414156.1 SOYC248TH LargeInsertSoybeanGenLibBuild4 Glycine max genomic clone H12A17:Build4MTP4H23, genomic survey sequence.    48   0.45   1
CE635221|CE635221.1 tigr-gss-dog-17000366743799 Dog Library Canis familiaris genomic, genomic survey sequence.    46   1.8    1
BZ298846|BZ298846.1 CG4616.f1 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG4616, DNA sequence.    46   1.8    1
BZ297417|BZ297417.1 CG3420.r1 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG3420, DNA sequence.    46   1.8    1
dna update 2007. 6.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P90521) RecName: Full=6-phosphofructokinase;          Short=Pho...   335   1e-90
CP001337_2617(CP001337|pid:none) Chloroflexus aggregans DSM 9485...   121   3e-26
BC081652_1(BC081652|pid:none) Danio rerio phosphofructokinase, m...   108   3e-22
(Q867C9) RecName: Full=6-phosphofructokinase, muscle type;      ...   104   5e-21
BC077756_1(BC077756|pid:none) Xenopus laevis MGC79063 protein, m...   103   8e-21
DQ480147_1(DQ480147|pid:none) Sus scrofa muscle 6-phosphofructok...   103   1e-20
AY335614_1(AY335614|pid:none) Synthetic construct Homo sapiens p...   102   2e-20
CR858939_1(CR858939|pid:none) Pongo abelii mRNA; cDNA DKFZp468O1...   102   2e-20
(P08237) RecName: Full=6-phosphofructokinase, muscle type;      ...   102   2e-20
(Q0IIG5) RecName: Full=6-phosphofructokinase, muscle type;      ...   102   2e-20
protein update 2009. 6.30
PSORT 

 psg: 0.03  gvh: 0.39  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.89  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: cytoplasmic
	  4.0 %: mitochondrial

>> prediction for VHE205 is nuc
5' end seq. ID VHE205F
5' end seq. 
>VHE205F.Seq
TTGGCCTACTGGAAACAATATATAAATCAATTAAAATAAACACTCACTCGATAGTGTAAA
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTN
NAAAAAAAAAANNNNCCCCCCCCCNAAAANNTTTTTTNNGGGGGGGGGGGGNNANNTTTN
GGGGGNNNAAAANNNNNNAAAAAAAANTTTTTTTNNGGGNAAANNTTTTTTNNAAAAAAA
AANNTTTTTTTNNGNNAAANNGGGNNNAAANCCCCCNTTTTTTNNAAAAAAANNGGGGGT
TTTNNNNNNCGGGGGGGGNANCCNGGGGNNNAANCCCCCNNNNNNANNNTTTTNCNNNNN
AATTTTTTTTNNNNGGGNNNNANTTTTTTNNNTTNGGGGGGGGNTNNNNGGGGGGGGNAA
NAAANNNNNNNNNN
Length of 5' end seq. 434
3' end seq. ID VHE205Z
3' end seq. 
>VHE205Z.Seq
NNNNNNNNNNCATATATAATGGAAAGAGGTATCACATTGAATACACTAACCAAGGATTTG
GAAATGTTTGTTGAACGTTTCAAACGTGAACATAGAATTGGTCTAATTATTAAGAGTGAA
CTTGCTAGCAACACCTACTCAACTCATTTCATTTATTCACTCTTCAAAGAAGAAGGTAAA
CATTTATTCGATGTCCGTGAATCTATACTCGGTCATTTACAACAAGGTGGTACTCCTTCT
GCAATTGATCGTATCTTTTCAACTCGTTTAATGAATCATTATTATCAATTCTTGGAAAAC
GATCTCAAAGAACATGGTCATCTTCAAATGAATGGTTGTATTGGTTTCATTGATGGTGGT
ATTCATTACACTCCAATGCAAGAAATGATCGAAGAAATGTCTGATAAATTTAGACGTCCT
CGTTCCCAATGGTGGATGGATTTGGTTGAAACCTCTCAAAATATCTCTGTTTTCCCTTTA
GATGACCCTTCCTCTACT
Length of 3' end seq. 498
Connected seq. ID VHE205P
Connected seq. 
>VHE205P.Seq
TTGGCCTACTGGAAACAATATATAAATCAATTAAAATAAACACTCACTCGATAGTGTAAA
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTN
NAAAAAAAAAANNNNCCCCCCCCCNAAAANNTTTTTTNNGGGGGGGGGGGGNNANNTTTN
GGGGGNNNAAAANNNNNNAAAAAAAANTTTTTTTNNGGGNAAANNTTTTTTNNAAAAAAA
AANNTTTTTTTNNGNNAAANNGGGNNNAAANCCCCCNTTTTTTNNAAAAAAANNGGGGGT
TTTNNNNNNCGGGGGGGGNANCCNGGGGNNNAANCCCCCNNNNNNANNNTTTTNCNNNNN
AATTTTTTTTNNNNGGGNNNNANTTTTTTNNNTTNGGGGGGGGNTNNNNGGGGGGGGNAA
NAAA----------CATATATAATGGAAAGAGGTATCACATTGAATACACTAACCAAGGA
TTTGGAAATGTTTGTTGAACGTTTCAAACGTGAACATAGAATTGGTCTAATTATTAAGAG
TGAACTTGCTAGCAACACCTACTCAACTCATTTCATTTATTCACTCTTCAAAGAAGAAGG
TAAACATTTATTCGATGTCCGTGAATCTATACTCGGTCATTTACAACAAGGTGGTACTCC
TTCTGCAATTGATCGTATCTTTTCAACTCGTTTAATGAATCATTATTATCAATTCTTGGA
AAACGATCTCAAAGAACATGGTCATCTTCAAATGAATGGTTGTATTGGTTTCATTGATGG
TGGTATTCATTACACTCCAATGCAAGAAATGATCGAAGAAATGTCTGATAAATTTAGACG
TCCTCGTTCCCAATGGTGGATGGATTTGGTTGAAACCTCTCAAAATATCTCTGTTTTCCC
TTTAGATGACCCTTCCTCTACT
Length of connected seq. 912
Full length Seq ID -
Full length Seq. -
Length of full length seq. -