>VHE192 (VHE192Q) /CSM/VH/VHE1-D/VHE192Q.Seq.d/
TACCAAAAGAATTAACTATTGATCAAATTTTACAATCTCTTCCAAAAGAAGTTTTTGAAA
CAAGTAATTTTAAATCAGCATGTTCACTTTTAATAACAATCTTATTCATTGGTTTAAGTG
CAACATTCATTACTCTCATGCCATCTTACTTAACCCCAATTGGTTGGATGTTAATGGGTG
CCTCAGTTACTGGTTTAATGGTAATTGGTAATGATTGTATTCATAAAACTTTTTCAAAGA
ATCCATTAGTTAATTCAATTATTGGAACAATTGTAATGTTACCATTGTTATATCCATTCC
AATCTTACAAGATTTCAGAAAAGAATAATGCAGAAAAGAAATCGACAGCTGTTTTAAATG
GTGAACAATTCAAGATTAATGCACATACCACAACCGAAAGTACAAATCCAATTAGACAAT
GGGCAACTGGTAAATTCTTTTGGATTTTATCAATTATTAATTGGTCTGAAAATTATTTCT
CAACTAAAAATTTATCAGATAAAAAAGATAAAACCAAAGCAATCGTTTCAATTGCAATTG
TTTACATTTTTGGATTTATTTTCTTCCCAATGATGTTTAAATTTGTAGGTGTAAGTGGAT
TCCXXXXXXXXXXATCCATGGGTTATGTGGCAACATAAACATTACATTAAAATCGCCACT
TTTATGGGTCTCGTTTTCCCAACATTGGTAGCTGGTTTAGGTTGGGGTGATTGGGCTGGT
GGTTATTTCTACTCTGGTGTACTTCGTCTCGTTACAGTTCAACATTCCACATTCTTGGTC
AATTCATTGGCTCATTATTTAGGTGACTCTCCATACGATGATGAACATACTCCAAAGGAT
TCAGTTGTGACTGCTATATTAACATTTGGTGAAGGTTATCATAACTTCCATCATGAATTC
CCAAATGACTATAGAAACGCTTATAAATTCTATCAATATGATCCAACTAAATGGTTGATC
TCTGCAATGTATTATTTAGGCTTGGCTTATGATTTGAAAACTTTCTCCAAAAATGAAATT
GAAAAAGGTATGATTCAAATGCACCAAAAACATTTAGACGCAAAGAAACAATCAATCTAT
TGGGGTATCAATAAAGAAGATTTACCATCAATGACAATCGATGAATTTAATAATTTGGTT
GAAAAAGAAAATAAAAAGTTAATGATAATCAACAAAACCGTATTAGATGTCGAATCTTTT
GTTAATGATCACCCAGGTGGTTTAGCTTACATTAAAATGGGTATTGGTAAAGATGCAACT
TCAATGTTTACTGGTGAAGTTTATGCTCATTCAAATGCTGCTAAAAATTTATTATGTCAA
TTTTCAATGCTAAAATT

PKELTIDQILQSLPKEVFETSNFKSACSLLITILFIGLSATFITLMPSYLTPIGWMLMGA
SVTGLMVIGNDCIHKTFSKNPLVNSIIGTIVMLPLLYPFQSYKISEKNNAEKKSTAVLNG
EQFKINAHTTTESTNPIRQWATGKFFWILSIINWSENYFSTKNLSDKKDKTKAIVSIAIV
YIFGFIFFPMMFKFVGVSGF---

---PWVMWQHKHYIKIATFMGLVFPTLVAGLGWGDWAGGYFYSGVLRLVTVQHSTFLVNS
LAHYLGDSPYDDEHTPKDSVVTAILTFGEGYHNFHHEFPNDYRNAYKFYQYDPTKWLISA
MYYLGLAYDLKTFSKNEIEKGMIQMHQKHLDAKKQSIYWGINKEDLPSMTIDEFNNLVEK
ENKKLMIINKTVLDVESFVNDHPGGLAYIKMGIGKDATSMFTGEVYAHSNAAKNLLCQFS
MLK

Frame A:
yqkn*llikfynlfqkkflkqvilnqhvhf**qsyslv*vqhsllschlt*pqlvgc*wv
pqllv*w*lvmivfiklfqrih*liqlleql*cyhcyihsnltrfqkrimqkrnrqlf*m
vnnsrlmhipqpkvqiqldngqlvnsfgfyqlliglkiisqlkiyqikkikpkqsfqlql
ftfldlfssq*clnl*v*vds---

---ihglcgninitlkspllwvsfsqhw*lv*vgviglvvistlvyfvslqfniphswsi
hwlii*vtlhtmmnilqriql*lly*hlvkviitsimnsqmtietlinsinmiqlng*sl
qcii*awlmi*klspkmklkkv*fkctkni*tqrnnqsigvsikkiyhq*qsmnliiwlk
kkiks***stkpy*msnlllmitqvv*ltlkwvlvkmqlqcllvkfmliqmllkiyyvnf
qc*n

Frame B:
tkriny*snftisskrsf*nk*f*ismftfnnnlihwfkcnihyshaillnpnwldvngc
lsywfngnw**lys*nffkesis*fnywnncnvtivisipilqdfrke*crkeidscfkw
*tiqd*ctyhnrkyksn*tmgnw*illdfiny*lv*klfln*kfir*kr*nqsnrfncnc
lhfwiyflpndv*icrckwi---

---smgyvat*tlh*nrhfygsrfpnigswfrlg*lgwwlfllwctssrysstfhilgqf
igslfr*lsir**tyskgfscdcyiniw*rls*lps*ipk*l*krl*ilsi*sn*mvdlc
nvlfrlgl*fenflqk*n*krydsnapktfrrketinllgyq*rrftindnr*i**fg*k
rk*kvndnqqnrircrifc**sprwfslh*ngyw*rcnfnvyw*slcsfkcc*kfimsif
naki

Frame C:
PKELTIDQILQSLPKEVFETSNFKSACSLLITILFIGLSATFITLMPSYLTPIGWMLMGA
SVTGLMVIGNDCIHKTFSKNPLVNSIIGTIVMLPLLYPFQSYKISEKNNAEKKSTAVLNG
EQFKINAHTTTESTNPIRQWATGKFFWILSIINWSENYFSTKNLSDKKDKTKAIVSIAIV
YIFGFIFFPMMFKFVGVSGF---

---PWVMWQHKHYIKIATFMGLVFPTLVAGLGWGDWAGGYFYSGVLRLVTVQHSTFLVNS
LAHYLGDSPYDDEHTPKDSVVTAILTFGEGYHNFHHEFPNDYRNAYKFYQYDPTKWLISA
MYYLGLAYDLKTFSKNEIEKGMIQMHQKHLDAKKQSIYWGINKEDLPSMTIDEFNNLVEK
ENKKLMIINKTVLDVESFVNDHPGGLAYIKMGIGKDATSMFTGEVYAHSNAAKNLLCQFS
MLK

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE192 (VHE192Q) /CSM/VH/VHE1-D/VHE192Q.Seq.d/                       2202   0.0
VHQ401 (VHQ401Q) /CSM/VH/VHQ4-A/VHQ401Q.Seq.d/                       1178   0.0
VHA229 (VHA229Q) /CSM/VH/VHA2-B/VHA229Q.Seq.d/                       1162   0.0
VHD425 (VHD425Q) /CSM/VH/VHD4-B/VHD425Q.Seq.d/                       1160   0.0
VHH531 (VHH531Q) /CSM/VH/VHH5-B/VHH531Q.Seq.d/                       1076   0.0
VHP282 (VHP282Q) /CSM/VH/VHP2-D/VHP282Q.Seq.d/                       1072   0.0
VHK171 (VHK171Q) /CSM/VH/VHK1-C/VHK171Q.Seq.d/                       1047   0.0
VHB541 (VHB541Q) /CSM/VH/VHB5-B/VHB541Q.Seq.d/                       1047   0.0
VFM428 (VFM428Q) /CSM/VF/VFM4-B/VFM428Q.Seq.d/                       1047   0.0
VHD470 (VHD470Q) /CSM/VH/VHD4-C/VHD470Q.Seq.d/                       1043   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.  1047   0.0    2
DY893926|DY893926.1 CeleSEQ13391 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ13391, mRNA sequence.    48   2e-14  6
AL033501|AL033501.1 C.albicans cosmid Ca41C10.                     72   2e-11  2
DY886427|DY886427.1 CeleSEQ2079 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ2079, mRNA sequence.    50   4e-11  3
DY892961|DY892961.1 CeleSEQ12104 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ12104, mRNA sequence.    50   4e-11  3
DY895351|DY895351.1 CeleSEQ15152 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ15152, mRNA sequence.    48   2e-10  4
DY885896|DY885896.1 CeleSEQ991 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ991, mRNA sequence.    48   3e-10  4
DY888809|DY888809.1 CeleSEQ6138 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ6138, mRNA sequence.    50   6e-10  3
DY886077|DY886077.1 CeleSEQ1581 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ1581, mRNA sequence.    48   1e-09  4
AL396412|AL396412.1 T3 end of clone AR0AA030B05 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    54   6e-08  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116984_117(AC116984|pid:none) Dictyostelium discoideum chromos...   506   e-142
Y18554_1(Y18554|pid:none) Mortierella alpina ole2 gene for stear...   307   5e-82
AF085500_1(AF085500|pid:none) Mortierella alpina delta nine desa...   304   4e-81
Y18553_1(Y18553|pid:none) Mortierella alpina ole1 gene for stear...   303   9e-81
AY576801_1(AY576801|pid:none) Hortaea werneckii putative delta-9...   299   1e-79
AB195980_1(AB195980|pid:none) Mortierella alpina gene for omega9...   298   4e-79
DQ901954_1(DQ901954|pid:none) Aureobasidium pullulans putative d...   295   2e-78
S52746(S52746;S52745) stearoyl-CoA 9-desaturase (EC 1.14.19.1) [...   291   4e-77
(Q12618) RecName: Full=Acyl-CoA desaturase;          EC=1.14.19....   290   6e-77
AP007162_283(AP007162|pid:none) Aspergillus oryzae RIB40 genomic...   290   6e-77

 psg: 0.75  gvh: 0.67  alm: 0.22  top: 0.63  tms: 0.33  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 40.0 %: plasma membrane
	 12.0 %: nuclear
	 12.0 %: endoplasmic reticulum
	 12.0 %: mitochondrial
	 12.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for VHE192 is pla

>VHE192F.Seq
TACCAAAAGAATTAACTATTGATCAAATTTTACAATCTCTTCCAAAAGAAGTTTTTGAAA
CAAGTAATTTTAAATCAGCATGTTCACTTTTAATAACAATCTTATTCATTGGTTTAAGTG
CAACATTCATTACTCTCATGCCATCTTACTTAACCCCAATTGGTTGGATGTTAATGGGTG
CCTCAGTTACTGGTTTAATGGTAATTGGTAATGATTGTATTCATAAAACTTTTTCAAAGA
ATCCATTAGTTAATTCAATTATTGGAACAATTGTAATGTTACCATTGTTATATCCATTCC
AATCTTACAAGATTTCAGAAAAGAATAATGCAGAAAAGAAATCGACAGCTGTTTTAAATG
GTGAACAATTCAAGATTAATGCACATACCACAACCGAAAGTACAAATCCAATTAGACAAT
GGGCAACTGGTAAATTCTTTTGGATTTTATCAATTATTAATTGGTCTGAAAATTATTTCT
CAACTAAAAATTTATCAGATAAAAAAGATAAAACCAAAGCAATCGTTTCAATTGCAATTG
TTTACATTTTTGGATTTATTTTCTTCCCAATGATGTTTAAATTTGTAGGTGTAAGTGGAT
TCCNNNNNNNNNN

>VHE192Z.Seq
NNNNNNNNNNATCCATGGGTTATGTGGCAACATAAACATTACATTAAAATCGCCACTTTT
ATGGGTCTCGTTTTCCCAACATTGGTAGCTGGTTTAGGTTGGGGTGATTGGGCTGGTGGT
TATTTCTACTCTGGTGTACTTCGTCTCGTTACAGTTCAACATTCCACATTCTTGGTCAAT
TCATTGGCTCATTATTTAGGTGACTCTCCATACGATGATGAACATACTCCAAAGGATTCA
GTTGTGACTGCTATATTAACATTTGGTGAAGGTTATCATAACTTCCATCATGAATTCCCA
AATGACTATAGAAACGCTTATAAATTCTATCAATATGATCCAACTAAATGGTTGATCTCT
GCAATGTATTATTTAGGCTTGGCTTATGATTTGAAAACTTTCTCCAAAAATGAAATTGAA
AAAGGTATGATTCAAATGCACCAAAAACATTTAGACGCAAAGAAACAATCAATCTATTGG
GGTATCAATAAAGAAGATTTACCATCAATGACAATCGATGAATTTAATAATTTGGTTGAA
AAAGAAAATAAAAAGTTAATGATAATCAACAAAACCGTATTAGATGTCGAATCTTTTGTT
AATGATCACCCAGGTGGTTTAGCTTACATTAAAATGGGTATTGGTAAAGATGCAACTTCA
ATGTTTACTGGTGAAGTTTATGCTCATTCAAATGCTGCTAAAAATTTATTATGTCAATTT
TCAATGCTAAAATT

>VHE192P.Seq
TACCAAAAGAATTAACTATTGATCAAATTTTACAATCTCTTCCAAAAGAAGTTTTTGAAA
CAAGTAATTTTAAATCAGCATGTTCACTTTTAATAACAATCTTATTCATTGGTTTAAGTG
CAACATTCATTACTCTCATGCCATCTTACTTAACCCCAATTGGTTGGATGTTAATGGGTG
CCTCAGTTACTGGTTTAATGGTAATTGGTAATGATTGTATTCATAAAACTTTTTCAAAGA
ATCCATTAGTTAATTCAATTATTGGAACAATTGTAATGTTACCATTGTTATATCCATTCC
AATCTTACAAGATTTCAGAAAAGAATAATGCAGAAAAGAAATCGACAGCTGTTTTAAATG
GTGAACAATTCAAGATTAATGCACATACCACAACCGAAAGTACAAATCCAATTAGACAAT
GGGCAACTGGTAAATTCTTTTGGATTTTATCAATTATTAATTGGTCTGAAAATTATTTCT
CAACTAAAAATTTATCAGATAAAAAAGATAAAACCAAAGCAATCGTTTCAATTGCAATTG
TTTACATTTTTGGATTTATTTTCTTCCCAATGATGTTTAAATTTGTAGGTGTAAGTGGAT
TCC----------ATCCATGGGTTATGTGGCAACATAAACATTACATTAAAATCGCCACT
TTTATGGGTCTCGTTTTCCCAACATTGGTAGCTGGTTTAGGTTGGGGTGATTGGGCTGGT
GGTTATTTCTACTCTGGTGTACTTCGTCTCGTTACAGTTCAACATTCCACATTCTTGGTC
AATTCATTGGCTCATTATTTAGGTGACTCTCCATACGATGATGAACATACTCCAAAGGAT
TCAGTTGTGACTGCTATATTAACATTTGGTGAAGGTTATCATAACTTCCATCATGAATTC
CCAAATGACTATAGAAACGCTTATAAATTCTATCAATATGATCCAACTAAATGGTTGATC
TCTGCAATGTATTATTTAGGCTTGGCTTATGATTTGAAAACTTTCTCCAAAAATGAAATT
GAAAAAGGTATGATTCAAATGCACCAAAAACATTTAGACGCAAAGAAACAATCAATCTAT
TGGGGTATCAATAAAGAAGATTTACCATCAATGACAATCGATGAATTTAATAATTTGGTT
GAAAAAGAAAATAAAAAGTTAATGATAATCAACAAAACCGTATTAGATGTCGAATCTTTT
GTTAATGATCACCCAGGTGGTTTAGCTTACATTAAAATGGGTATTGGTAAAGATGCAACT
TCAATGTTTACTGGTGAAGTTTATGCTCATTCAAATGCTGCTAAAAATTTATTATGTCAA
TTTTCAATGCTAAAATT