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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE188
Library VH
(Link to library)
Clone ID VHE188
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15977-1|Contig-U16210-1
Original site URL
Representative seq. ID VHE188P
(Link to Original site)
Representative DNA sequence 
>VHE188 (VHE188Q) /CSM/VH/VHE1-D/VHE188Q.Seq.d/
ACAATATAAAAAATATAGAAAAAAATGAAATTAATTATATTAATTGTATTAAGCATTTTA
ATATCAATTGTAATTTCAGGATCAGTACAAAGTAAAATTACTACAACACGTGGTATTAGT
AATGTATACAGTAAAAATGATATAAATAATATAAAACAATTCATTTCAAGTAAATACAAT
GGTAATACCAAATTATATGGTGAATCATTAAAGGATACATTTTATGGAGTTGGAGTTTTA
ACACGTATTGGTGAAACAAATATTGAAGCAACAAAAGACATTTGTAAACAAACCAAAGAA
CAATTAAAACAAAATAAATTTACAGATATTGAATTAGTTTTCAATGGAGTTACTATCCTT
TCAGAATTAAAATGTTTACAAGGTGAATCATCATCATCATTGGGAAATGAACAACAATTA
CAAGATTTATTAAAAAATAAATTAGAGAATGGTTCATTATTGGAAAAGACACAAGCTATT
AATATTTATTTCACACTTTCAAGTGCAAAAGCAATCGATTCAAAAACCGTTTCAATAATT
GATCCATTATTGATACAAGCTGTTAATTCAATGGTTTCACTCATGGAXXXXXXXXXXTTA
TCATATGCACCAAAATCCGATCAATTAGGTTCTCCAAAGACTACCAATGAAGTTCAATTT
GGTCAAAAATTACCATTAATTGAAATCCCATCAAATAATATCGCTCGTATCTTCTTTAGA
ATCGCATCTGAAGCTCAACCATATCAAGCTCAACAAGTTGGTATTCGTTTCTATTCACCA
GCTAGAGAAGCCGTAGTTCCAGCAACTTACTCTGCTGATGCCCACTCTTACACCTTCACC
AATAAAGATGCTTGCAAGATTTTAGGTTGTCNAATCTGGAAATTATCAATTGGATTTAAT
CATTGGTGATCAATCAATCACTCCATTACAATGGAATTTCGGTGAAATTAATTTGAAATT
CAATCAATCAACCATTCCAACCAATCGTTATCCAGAACAACTCCCAATCTCTCACAACTT
TAGAGTAGCTGAAAAGAGACCACCACAATCTATCTCAAGTTTATTCACTCTCTTGGTCTT
ATCACCAATCGCCATCTTTGTCATTGGTCTCTTATTCGTTGGTACCAATCTTGGTAGATT
CCCAACTGGTATGGGTTTCATTTACACCATTGGTTTCCTTGGTTGTATCTCTGCAACTGG
TCTCTTAATTGTAAACTATTGGTTACACTCCACAATGGATGTTACCCTCAAAAATTTAGC
TTTATTAATGATTCCATTAGTTTTCTTNGGTCATAAATCAATGAGTTATTATTCAAATCT
TTCTTCTTCAAATATTAAAAAAGNATTAAAAATAAAAAATAA
sequence update 2002.10.25
Translated Amino Acid sequence 
ti*ki*KKMKLIILIVLSILISIVISGSVQSKITTTRGISNVYSKNDINNIKQFISSKYN
GNTKLYGESLKDTFYGVGVLTRIGETNIEATKDICKQTKEQLKQNKFTDIELVFNGVTIL
SELKCLQGESSSSLGNEQQLQDLLKNKLENGSLLEKTQAINIYFTLSSAKAIDSKTVSII
DPLLIQAVNSMVSLM---

---LSYAPKSDQLGSPKTTNEVQFGQKLPLIEIPSNNIARIFFRIASEAQPYQAQQVGIR
FYSPAREAVVPATYSADAHSYTFTNKDACKILGCXIWKLSIGFNHW*sinhsitmefr*n
*feiqsinhsnqslsrttpnlsql*ss*ketttiylkfihslglitnrhlchwslirwyq
sw*ipnwygfhlhhwfpwlylcnwslnckllvtlhngcypqkfsfindsisflxs*inel
lfksfffky*kxiknkk*
Translated Amino Acid sequence (All Frames) 
Frame A:
ti*ki*KKMKLIILIVLSILISIVISGSVQSKITTTRGISNVYSKNDINNIKQFISSKYN
GNTKLYGESLKDTFYGVGVLTRIGETNIEATKDICKQTKEQLKQNKFTDIELVFNGVTIL
SELKCLQGESSSSLGNEQQLQDLLKNKLENGSLLEKTQAINIYFTLSSAKAIDSKTVSII
DPLLIQAVNSMVSLM---

---LSYAPKSDQLGSPKTTNEVQFGQKLPLIEIPSNNIARIFFRIASEAQPYQAQQVGIR
FYSPAREAVVPATYSADAHSYTFTNKDACKILGCXIWKLSIGFNHW*sinhsitmefr*n
*feiqsinhsnqslsrttpnlsql*ss*ketttiylkfihslglitnrhlchwslirwyq
sw*ipnwygfhlhhwfpwlylcnwslnckllvtlhngcypqkfsfindsisflxs*inel
lfksfffky*kxiknkk*

Frame B:
qykkyrkk*n*ly*ly*af*yql*fqdqykvkllqhvvlvmytvkmi*ii*nnsfqvntm
vipnymvnh*rihfmelef*hvlvkqilkqqktfvnkpknn*nkinlqiln*fsmellsf
qn*nvykvnhhhhwemnnnykiy*kin*rmvhywkrhkllifishfqvqkqsiqkpfq*l
ihy*yklliqwfhsw---

---yhmhqnpin*vlqrlpmkfnlvknyh*lkshqiislvssleshlklnhiklnklvfv
sihqlekp*fqqltllmptltpspikmlarf*vvxsgnyqldliigdqsitplqwnfgei
nlkfnqstiptnrypeqlpishnfrvaekrppqsisslftllvlspiaifvigllfvgtn
lgrfptgmgfiytigflgcisatgllivnywlhstmdvtlknlallmiplvfxghksmsy
ysnlsssnikkxlkikn

Frame C:
nikniekneinyincikhfnincnfristk*nyyntwy**ciq*k*yk*yktihfk*iqw
*yqiiw*iikgyilwswsfntyw*nky*snkrhl*tnqrtiktk*iyry*isfqwsyypf
rikmftr*iiiiigk*ttitrfikk*irewfiigkdtsy*ylfhtfkcksnrfknrfnn*
siidtsc*fngfthg---

---iictkirsirfskdyq*ssiwskitin*npik*yrsyll*nri*sstissstswysf
lfts*rsrsssnllc*cpllhlhq*rclqdfrlsnleiinwi*slvinqslhyngisvkl
i*nsinqpfqpiviqnnsqslttle*lkrdhhnlsqvyslswsyhqspslslvsyslvpi
lvdsqlvwvsftplvslvvslqlvs*l*tigytpqwmlpski*ly**fh*fsxvinq*vi
iqifllqilkkx*k*ki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE188 (VHE188Q) /CSM/VH/VHE1-D/VHE188Q.Seq.d/                       2535   0.0
VHQ120 (VHQ120Q) /CSM/VH/VHQ1-A/VHQ120Q.Seq.d/                       1435   0.0
VHP310 (VHP310Q) /CSM/VH/VHP3-A/VHP310Q.Seq.d/                       1435   0.0
VHO870 (VHO870Q) /CSM/VH/VHO8-C/VHO870Q.Seq.d/                       1427   0.0
VHC534 (VHC534Q) /CSM/VH/VHC5-B/VHC534Q.Seq.d/                       1427   0.0
VHD225 (VHD225Q) /CSM/VH/VHD2-B/VHD225Q.Seq.d/                       1419   0.0
VHC739 (VHC739Q) /CSM/VH/VHC7-B/VHC739Q.Seq.d/                       1419   0.0
VFJ757 (VFJ757Q) /CSM/VF/VFJ7-C/VFJ757Q.Seq.d/                       1306   0.0
VHM302 (VHM302Q) /CSM/VH/VHM3-A/VHM302Q.Seq.d/                       1291   0.0
AFN258 (AFN258Q) /CSM/AF/AFN2-C/AFN258Q.Seq.d/                       1281   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    38   0.001  13
CR339063|CR339063.8 Zebrafish DNA sequence from clone CH211-247J10 in linkage group 20.    44   0.007  2
AC115607|AC115607.2 Dictyostelium discoideum chromosome 2 map 4559693-4624405 strain AX4, complete sequence.    34   0.037  9
AC141704|AC141704.1 Apis mellifera clone CH224-56H9, WORKING DRAFT SEQUENCE, 25 unordered pieces.    50   0.17   1
AC101655|AC101655.1 Mus musculus clone RP23-201D5, LOW-PASS SEQUENCE SAMPLING.    50   0.17   1
EI479448|EI479448.1 PV_GBa0091G09.f PV_GBa Phaseolus vulgaris genomic clone PV_GBa0091G09 5', genomic survey sequence.    38   0.21   2
AM445580|AM445580.2 Vitis vinifera contig VV78X248860.2, whole genome shotgun sequence.    42   0.30   2
AE014827|AE014827.1 Plasmodium falciparum 3D7 chromosome 14 section 12 of 13 of the complete sequence.    36   0.36   8
AC176336|AC176336.1 Strongylocentrotus purpuratus clone R3-3053M2, WORKING DRAFT SEQUENCE, 8 unordered pieces.    36   0.37   2
AC117070|AC117070.2 Dictyostelium discoideum chromosome 2 map 2097701-2233941 strain AX4, complete sequence.    34   0.38   9
dna update 2007. 6.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY058845_1(AY058845|pid:none) Arabidopsis thaliana AT4g21150/F7J...    84   9e-15
AK315801_1(AK315801|pid:none) Homo sapiens cDNA, FLJ96923, highl...    79   4e-13
AK096243_1(AK096243|pid:none) Homo sapiens cDNA FLJ38924 fis, cl...    79   4e-13
(P04844) RecName: Full=Dolichyl-diphosphooligosaccharide--protei...    79   4e-13
DQ895552_1(DQ895552|pid:none) Synthetic construct Homo sapiens c...    79   4e-13
B26168(B26168)ribophorin II precursor - human                          79   4e-13
Y00282_1(Y00282|pid:none) Human mRNA for ribophorin II.                79   4e-13
BC002380_1(BC002380|pid:none) Homo sapiens ribophorin II, mRNA (...    79   4e-13
(P25235) RecName: Full=Dolichyl-diphosphooligosaccharide--protei...    78   6e-13
CR860502_1(CR860502|pid:none) Pongo abelii mRNA; cDNA DKFZp468G2...    77   1e-12
protein update 2009. 6.30
PSORT 

 psg: 0.97  gvh: 0.68  alm: 0.37  top: 0.47  tms: 0.00  mit: 0.39  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: extracellular, including cell wall
	 20.0 %: mitochondrial
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic
	  4.0 %: Golgi
	  4.0 %: vacuolar

>> prediction for VHE188 is exc
5' end seq. ID VHE188F
5' end seq. 
>VHE188F.Seq
ACAATATAAAAAATATAGAAAAAAATGAAATTAATTATATTAATTGTATTAAGCATTTTA
ATATCAATTGTAATTTCAGGATCAGTACAAAGTAAAATTACTACAACACGTGGTATTAGT
AATGTATACAGTAAAAATGATATAAATAATATAAAACAATTCATTTCAAGTAAATACAAT
GGTAATACCAAATTATATGGTGAATCATTAAAGGATACATTTTATGGAGTTGGAGTTTTA
ACACGTATTGGTGAAACAAATATTGAAGCAACAAAAGACATTTGTAAACAAACCAAAGAA
CAATTAAAACAAAATAAATTTACAGATATTGAATTAGTTTTCAATGGAGTTACTATCCTT
TCAGAATTAAAATGTTTACAAGGTGAATCATCATCATCATTGGGAAATGAACAACAATTA
CAAGATTTATTAAAAAATAAATTAGAGAATGGTTCATTATTGGAAAAGACACAAGCTATT
AATATTTATTTCACACTTTCAAGTGCAAAAGCAATCGATTCAAAAACCGTTTCAATAATT
GATCCATTATTGATACAAGCTGTTAATTCAATGGTTTCACTCATGGANNNNNNNNNN
Length of 5' end seq. 597
3' end seq. ID VHE188Z
3' end seq. 
>VHE188Z.Seq
NNNNNNNNNNTTATCATATGCACCAAAATCCGATCAATTAGGTTCTCCAAAGACTACCAA
TGAAGTTCAATTTGGTCAAAAATTACCATTAATTGAAATCCCATCAAATAATATCGCTCG
TATCTTCTTTAGAATCGCATCTGAAGCTCAACCATATCAAGCTCAACAAGTTGGTATTCG
TTTCTATTCACCAGCTAGAGAAGCCGTAGTTCCAGCAACTTACTCTGCTGATGCCCACTC
TTACACCTTCACCAATAAAGATGCTTGCAAGATTTTAGGTTGTCNAATCTGGAAATTATC
AATTGGATTTAATCATTGGTGATCAATCAATCACTCCATTACAATGGAATTTCGGTGAAA
TTAATTTGAAATTCAATCAATCAACCATTCCAACCAATCGTTATCCAGAACAACTCCCAA
TCTCTCACAACTTTAGAGTAGCTGAAAAGAGACCACCACAATCTATCTCAAGTTTATTCA
CTCTCTTGGTCTTATCACCAATCGCCATCTTTGTCATTGGTCTCTTATTCGTTGGTACCA
ATCTTGGTAGATTCCCAACTGGTATGGGTTTCATTTACACCATTGGTTTCCTTGGTTGTA
TCTCTGCAACTGGTCTCTTAATTGTAAACTATTGGTTACACTCCACAATGGATGTTACCC
TCAAAAATTTAGCTTTATTAATGATTCCATTAGTTTTCTTNGGTCATAAATCAATGAGTT
ATTATTCAAATCTTTCTTCTTCAAATATTAAAAAAGNATTAAAAATAAAAAATAA
Length of 3' end seq. 775
Connected seq. ID VHE188P
Connected seq. 
>VHE188P.Seq
ACAATATAAAAAATATAGAAAAAAATGAAATTAATTATATTAATTGTATTAAGCATTTTA
ATATCAATTGTAATTTCAGGATCAGTACAAAGTAAAATTACTACAACACGTGGTATTAGT
AATGTATACAGTAAAAATGATATAAATAATATAAAACAATTCATTTCAAGTAAATACAAT
GGTAATACCAAATTATATGGTGAATCATTAAAGGATACATTTTATGGAGTTGGAGTTTTA
ACACGTATTGGTGAAACAAATATTGAAGCAACAAAAGACATTTGTAAACAAACCAAAGAA
CAATTAAAACAAAATAAATTTACAGATATTGAATTAGTTTTCAATGGAGTTACTATCCTT
TCAGAATTAAAATGTTTACAAGGTGAATCATCATCATCATTGGGAAATGAACAACAATTA
CAAGATTTATTAAAAAATAAATTAGAGAATGGTTCATTATTGGAAAAGACACAAGCTATT
AATATTTATTTCACACTTTCAAGTGCAAAAGCAATCGATTCAAAAACCGTTTCAATAATT
GATCCATTATTGATACAAGCTGTTAATTCAATGGTTTCACTCATGGA----------TTA
TCATATGCACCAAAATCCGATCAATTAGGTTCTCCAAAGACTACCAATGAAGTTCAATTT
GGTCAAAAATTACCATTAATTGAAATCCCATCAAATAATATCGCTCGTATCTTCTTTAGA
ATCGCATCTGAAGCTCAACCATATCAAGCTCAACAAGTTGGTATTCGTTTCTATTCACCA
GCTAGAGAAGCCGTAGTTCCAGCAACTTACTCTGCTGATGCCCACTCTTACACCTTCACC
AATAAAGATGCTTGCAAGATTTTAGGTTGTCNAATCTGGAAATTATCAATTGGATTTAAT
CATTGGTGATCAATCAATCACTCCATTACAATGGAATTTCGGTGAAATTAATTTGAAATT
CAATCAATCAACCATTCCAACCAATCGTTATCCAGAACAACTCCCAATCTCTCACAACTT
TAGAGTAGCTGAAAAGAGACCACCACAATCTATCTCAAGTTTATTCACTCTCTTGGTCTT
ATCACCAATCGCCATCTTTGTCATTGGTCTCTTATTCGTTGGTACCAATCTTGGTAGATT
CCCAACTGGTATGGGTTTCATTTACACCATTGGTTTCCTTGGTTGTATCTCTGCAACTGG
TCTCTTAATTGTAAACTATTGGTTACACTCCACAATGGATGTTACCCTCAAAAATTTAGC
TTTATTAATGATTCCATTAGTTTTCTTNGGTCATAAATCAATGAGTTATTATTCAAATCT
TTCTTCTTCAAATATTAAAAAAGNATTAAAAATAAAAAATAA
Length of connected seq. 1352
Full length Seq ID -
Full length Seq. -
Length of full length seq. -