>AFN685 (AFN685Q) /CSM/AF/AFN6-D/AFN685Q.Seq.d/
TACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCA
AATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACC
TTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCAT
CAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTA
AAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTA
AATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAA
ATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTG
GTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAA
TGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGGTGGTTCACATGCAGGTAATAAA
TTAGCTATGCAAGAATTTATGATTTTACCAGTCGXXXXXXXXXXAATGTCATCAGTGGTA
GATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCCACCAATTCAAA
GCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGGTTACACAGGTT
TAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGTTACGATC
TTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGGTG
ATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGATC
AAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGTG
ATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCATGTA
ATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGCTC
TTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAG
ATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTC
CATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTTAG
GTGAAAACCATAACTATGCGGTTTAACTTTCCGTAAATTTTAATTTTACTCATAATATG

yfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhqvh
qlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismvpq
ikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*DYQFQHSMLSMGGSHAGNK
LAMQEFMILPV---

---NVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAASE
FKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLTA
SVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMV
SHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNYAV*lsv
nfnfthnm

Frame A:
yfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhqvh
qlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismvpq
ikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*DYQFQHSMLSMGGSHAGNK
LAMQEFMILPV---

---NVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAASE
FKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLTA
SVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMV
SHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNYAV*lsv
nfnfthnm

Frame B:
tlfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssirci
nwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrwypk
*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwvvhmqvin
*lcknl*fyqs---

---mssvvdtvkmlsmlvmkvvslhqfkatkkvlns*nlplkklvtqv*lklvwivlpln
sklkmvtiltsklktmtvqllslvkn*vistenslkniqsfqlkihlikmignhtln*qh
qlifkllvmiyllptqkelrlvlkrkhvmlfy*kltklvqslnqfvplltqrmhhgvswl
aivqvklkipslli*llv*vlvklklvlhvdqkd*pntinlleltks*vktitmrfnfp*
ilillii

Frame C:
lyfflslsiyisffkmsviksikareildsrgnptvevdlytekdgvvssfraavpsgas
tgiyeavelrdgdksrylgkgvlkaiknileviqpavigksvsdqaaidklmidldgtpn
kgklganailavslavcragaadrnlplykyiseiagtkmrlpvpafnvingwftcr**i
syariydfts---

---chqw*irsrcyqcw**rwfrstnskqqrrs*tlktch*kswlhrfs*nwyglccl*i
qs*kwlrs*lqn*kq*rfscylw*kir*slqrih*risnhfn*rsi*sr*lgiih*insi
s*ysncw**ftcyqprkn*dwy*kesm*csfiks*pnwfsh*inscrs*lkecimgchg*
psfr*n*rylhc*fscwfrywsn*nwcsm*irkisqiqstc*n*rrvr*kp*lcgltfrk
f*fys*y

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFN685 (AFN685Q) /CSM/AF/AFN6-D/AFN685Q.Seq.d/                       2524   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1457   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1443   0.0
SSI448 (SSI448Q) /CSM/SS/SSI4-B/SSI448Q.Seq.d/                       1443   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1443   0.0
SFC169 (SFC169Q) /CSM/SF/SFC1-C/SFC169Q.Seq.d/                       1443   0.0
CHB226 (CHB226Q) /CSM/CH/CHB2-B/CHB226Q.Seq.d/                       1443   0.0
AFK881 (AFK881Q) /CSM/AF/AFK8-D/AFK881Q.Seq.d/                       1443   0.0
AFG692 (AFG692Q) /CSM/AF/AFG6-D/AFG692Q.Seq.d/                       1443   0.0
SFB846 (SFB846Q) /CSM/SF/SFB8-B/SFB846Q.Seq.d/                       1437   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X80474|X80474.1 N.frontalis enolase gene.                         64  1e-76  13
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.          70  2e-68  11
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76  2e-57  10
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76  2e-57  10
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76  2e-57  10
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76  1e-55  9
BM395755|BM395755.1 5009-0-10-H08.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   121  1e-48  5
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92  1e-46  5
AF348930|AF348930.1 Tetrahymena bergeri enolase (eno) gene, partial cds.    66  2e-46  6
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.         50  8e-46  10

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   742   0.0
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   529   e-149
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   520   e-146
AK243111_1(AK243111|pid:none) Oryza sativa Japonica Group cDNA, ...   509   e-143
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   508   e-142
EU964132_1(EU964132|pid:none) Zea mays clone 276124 enolase mRNA...   507   e-142
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   506   e-142
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   506   e-142
T12341(T12341) phosphopyruvate hydratase (EC 4.2.1.11) - common ...   504   e-141
(Q43130) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   503   e-141

 psg: 0.75  gvh: 0.51  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFN685 is cyt

>AFN685F.Seq
TACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCA
AATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACC
TTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCAT
CAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTA
AAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTA
AATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAA
ATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTG
GTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAA
TGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGGTGGTTCACATGCAGGTAATAAA
TTAGCTATGCAAGAATTTATGATTTTACCAGTCG----------

>AFN685Z.Seq
----------AATGTCATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGATGA
AGGTGGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGC
CATTGAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGA
ATTCAAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGC
TGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAAT
CATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGC
ATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAA
GACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTC
AGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGT
TAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGG
TACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCA
ACTTGTTAGAATTAACGAAGAGTTAGGTGAAAACCATAACTATGCGGTTTAACTTTCCGT
AAATTTTAATTTTACTCATAATATG

>AFN685P.Seq
TACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCA
AATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACC
TTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCAT
CAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTA
AAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTA
AATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAA
ATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTG
GTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAA
TGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGGTGGTTCACATGCAGGTAATAAA
TTAGCTATGCAAGAATTTATGATTTTACCAGTCG----------AATGTCATCAGTGGTA
GATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCCACCAATTCAAA
GCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGGTTACACAGGTT
TAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGTTACGATC
TTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGGTG
ATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGATC
AAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGTG
ATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCATGTA
ATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGCTC
TTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAG
ATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTC
CATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTTAG
GTGAAAACCATAACTATGCGGTTTAACTTTCCGTAAATTTTAATTTTACTCATAATATG