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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE123
Library VH
(Link to library)
Clone ID VHE123
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11249-1|Contig-U12842-1
Original site URL
Representative seq. ID VHE123P
(Link to Original site)
Representative DNA sequence 
>VHE123 (VHE123Q) /CSM/VH/VHE1-A/VHE123Q.Seq.d/
AAAGAGATAAAATGCCCCAAAATAAAAGTCAAACAATAAAACTATCTCATTTTTTAAGAG
ATAATCTTCTTAACTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATGCAGAAG
ATGCTCGAGCTGATACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATAATGGGT
TGTTAACTTCAAATGATAACCAAGAAATTGAAAGTTCAAAAGAATTATTAGGTCCATCAA
TTTTAATTTATAATAATGCAACTTTTCAAGAACATGATTGGGAAGGAATTTGTCAAATTA
GTCAAGGTAGTAAAAAAGAAAATTTAAAAAGTGTTGGCAATTTCGGTTTAGGTTGGAACA
GTGTTTATCATATAACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGTTCTCAG
ATCCAAACGAAAGAATGAGTGGTGGTTTATTCTTTGATTTAGATGACGAAGATGATTATA
AGAAATGTTTTAGTTTTTTAGAGCCATTCATTCAATTTAAAGAATCAAATTGTAACCCAA
AAGAATATTTTAATGGTACAATCATTCGTCTACCATTGXXXXXXXXXXATTTTATACTCA
TAGCTTTCTTTACACGAGTGATCAAGTCTATGATCTCTTATTCAAATATTATGGTAATAA
GTTGGCCAAACATTTAAAATTTTCTACATTACCATTAAACTTTAGACAATTAATTTCATC
AATTTGTAGAAATTATATAAATATTAGAGAAATTAAACTTTCAGATTTTATCACTAGTCT
CAACTCCATCAACAACCTCCAATTTTTAAGTGATAGTATAATAACAATTTGGAATTGTAT
CGATGAATTAAAAAGAAAAGAAACTTTAGAATTATCTCATATCCAATCATTACAAATTAT
CCCATATAGATTAAGTCCAACTGAAACTGATGTCACTAGTTTTGATAAATCTTCATTGTT
AATGTACAAATCAAATTTCTTACCAATTGAACCAATCTTAAACAAACTAAAAATCTATTT
AATAAAAGAAGGACATCAAATCAATAGTTTATTAGATGGAAATGCTCAAATCCCAATTTT
ATTAGGCTATCTTCAAAGGGTAAATTTATCATTAGACTCAAAGGAAATCGATAAATTAAG
AATATTTTTAGTTGAATCAATGTTCCAAATTTCTTTATCACAAGATTATACTTCAATTTT
TTATAATTTACCAATTCACCCATGCATTGGAAATAGTAACCAAAAGTTTAGTAGTATTAC
AACCAGTAGTTACTGTGTGGATTAGAAAAAATGATCAATANAAATTCCAGGATTAAAATT
AATAAAGGGTTAANAATTAACAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
RDKMPQNKSQTIKLSHFLRDNLLNYPESKIFRELIQNAEDARADTVIIKVDEGSYPNNGL
LTSNDNQEIESSKELLGPSILIYNNATFQEHDWEGICQISQGSKKENLKSVGNFGLGWNS
VYHITDNPIVYSGNNVWFSDPNERMSGGLFFDLDDEDDYKKCFSFLEPFIQFKESNCNPK
EYFNGTIIRLPL---

---FYTHSFLYTSDQVYDLLFKYYGNKLAKHLKFSTLPLNFRQLISSICRNYINIREIKL
SDFITSLNSINNLQFLSDSIITIWNCIDELKRKETLELSHIQSLQIIPYRLSPTETDVTS
FDKSSLLMYKSNFLPIEPILNKLKIYLIKEGHQINSLLDGNAQIPILLGYLQRVNLSLDS
KEIDKLRIFLVESMFQISLSQDYTSIFYNLPIHPCIGNSNQKFSSITTSSYCVD*kk*si
xipglklikg*xltk
Translated Amino Acid sequence (All Frames) 
Frame A:
keikcpkikvkq*nylif*eiifltiqnqkylen*fkmqkmleliqs**rlmkahiqimg
c*lqmitkklkvqkny*vhqf*fiimqlfknmigkefvklvkvvkkki*kvlaisv*vgt
vfii*qiiqlfivvimfgsqiqtke*vvvysli*mtkmiirnvlvf*shsfnlknqivtq
knilmvqsfvyh---

---ilys*lslhe*ssl*sliqilw**vgqtfkifyitikl*tinfinl*klyky*rn*t
frfyh*sqlhqqppifk**ynnnlelyr*ikkkrnfriisypiitnypi*iksn*n*ch*
f**ifivnvqikfltn*tnlkqtknlfnkrrtsnq*firwkcsnpnfirlsskgkfiirl
kgnr*iknifs*invpnffitrlyfnfl*ftnspmhwk**pkv**yynq*llcglekmin
xnsrikinkglxink

Frame B:
kr*napk*ksnnktisffkr*ss*lsrikni*ridskcrrcss*yshnkg**rlisk*wv
vnfk**prn*kfkriirsinfnl**cnfsrt*lgrnlsn*sr**krkfkkcwqfrfrleq
clsynr*sncl*w**clvlrskrknewwfil*fr*rr*l*emf*ffraihsi*rikl*pk
rif*wynhssti---

---FYTHSFLYTSDQVYDLLFKYYGNKLAKHLKFSTLPLNFRQLISSICRNYINIREIKL
SDFITSLNSINNLQFLSDSIITIWNCIDELKRKETLELSHIQSLQIIPYRLSPTETDVTS
FDKSSLLMYKSNFLPIEPILNKLKIYLIKEGHQINSLLDGNAQIPILLGYLQRVNLSLDS
KEIDKLRIFLVESMFQISLSQDYTSIFYNLPIHPCIGNSNQKFSSITTSSYCVD*kk*si
xipglklikg*xltk

Frame C:
RDKMPQNKSQTIKLSHFLRDNLLNYPESKIFRELIQNAEDARADTVIIKVDEGSYPNNGL
LTSNDNQEIESSKELLGPSILIYNNATFQEHDWEGICQISQGSKKENLKSVGNFGLGWNS
VYHITDNPIVYSGNNVWFSDPNERMSGGLFFDLDDEDDYKKCFSFLEPFIQFKESNCNPK
EYFNGTIIRLPL---

---filiafftrviksmisysnimviswpni*nflhyh*tldn*fhqfveii*ileklnf
qilslvstpsttsnf*viv**qfgivsmn*kekkl*nylisnhyklshid*vqlklmslv
linlhc*ctnqisyqlnqs*tn*ksi**kkdiksivy*memlksqfy*aifkg*iyh*tq
rksin*eyf*lnqcskflyhkiilqffiiyqfthaleivtkslvvlqpvvtvwirkndqx
kfqd*n**rvxn*q
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE123 (VHE123Q) /CSM/VH/VHE1-A/VHE123Q.Seq.d/                       1941   0.0
VHD239 (VHD239Q) /CSM/VH/VHD2-B/VHD239Q.Seq.d/                       1439   0.0
VHB767 (VHB767Q) /CSM/VH/VHB7-C/VHB767Q.Seq.d/                       1435   0.0
VHO809 (VHO809Q) /CSM/VH/VHO8-A/VHO809Q.Seq.d/                       1427   0.0
VHK810 (VHK810Q) /CSM/VH/VHK8-A/VHK810Q.Seq.d/                       1394   0.0
VHK791 (VHK791Q) /CSM/VH/VHK7-D/VHK791Q.Seq.d/                       1342   0.0
VFN211 (VFN211Q) /CSM/VF/VFN2-A/VFN211Q.Seq.d/                       1176   0.0
VHN647 (VHN647Q) /CSM/VH/VHN6-B/VHN647Q.Seq.d/                        609   e-173
VHC102 (VHC102Q) /CSM/VH/VHC1-A/VHC102Q.Seq.d/                        609   e-173
VHE271 (VHE271Q) /CSM/VH/VHE2-C/VHE271Q.Seq.d/                        593   e-168
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CU271466|CU271466.1 Equus caballus GSS, BAC clone CH241-426B21, SP6 end sequence.    36   0.006  3
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    34   0.008  17
CU054628|CU054628.1 Equus caballus GSS, BAC clone CH241-155P22, SP6 end sequence.    36   0.009  3
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.            36   0.010  17
CP000123|CP000123.1 Mycoplasma capricolum subsp. capricolum ATCC 27343, complete genome.    32   0.032  22
AC006279|AC006279.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    38   0.041  12
AC025306|AC025306.3 Homo sapiens clone RP11-24F7, WORKING DRAFT SEQUENCE, 20 unordered pieces.    52   0.043  1
ED245306|ED245306.1 AUAC-aaq90h10.g1 Ascaris suum whole genome shotgun library (PMAJ_4 GSS) Ascaris suum genomic, genomic survey sequence.    52   0.043  1
AL392089|AL392089.7 Human DNA sequence from clone RP11-31F19 on chromosome 9p24.1-24.3.    52   0.043  1
AE014836|AE014836.1 Plasmodium falciparum 3D7 chromosome 11 section 1 of 8 of the complete sequence.    42   0.043  13
dna update 2007. 6.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9NZJ4) RecName: Full=Sacsin;  &AL157766_4(AL157766|pid:none)        105   4e-21
BC142916_1(BC142916|pid:none) Danio rerio hypothetical LOC555303...   105   4e-21
CP001577_292(CP001577|pid:none) Micromonas sp. RCC299 chromosome...   103   1e-20
BC138482_1(BC138482|pid:none) Mus musculus sacsin, mRNA (cDNA cl...   103   1e-20
(Q9JLC8) RecName: Full=Sacsin;                                        103   1e-20
BC171956_1(BC171956|pid:none) Mus musculus sacsin, mRNA (cDNA cl...   103   1e-20
AB006708_10(AB006708|pid:none) Arabidopsis thaliana genomic DNA,...   100   2e-19
AL732351_6(AL732351|pid:none) Oryza sativa genomic DNA, chromoso...    90   2e-16
AL606457_18(AL606457|pid:none) Oryza sativa genomic DNA, chromos...    89   3e-16
CR749427_1(CR749427|pid:none) Homo sapiens mRNA; cDNA DKFZp686B1...    77   2e-12
protein update 2009. 6.30
PSORT 

 psg: 0.68  gvh: 0.39  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	 24.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VHE123 is cyt
5' end seq. ID VHE123F
5' end seq. 
>VHE123F.Seq
AAAGAGATAAAATGCCCCAAAATAAAAGTCAAACAATAAAACTATCTCATTTTTTAAGAG
ATAATCTTCTTAACTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATGCAGAAG
ATGCTCGAGCTGATACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATAATGGGT
TGTTAACTTCAAATGATAACCAAGAAATTGAAAGTTCAAAAGAATTATTAGGTCCATCAA
TTTTAATTTATAATAATGCAACTTTTCAAGAACATGATTGGGAAGGAATTTGTCAAATTA
GTCAAGGTAGTAAAAAAGAAAATTTAAAAAGTGTTGGCAATTTCGGTTTAGGTTGGAACA
GTGTTTATCATATAACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGTTCTCAG
ATCCAAACGAAAGAATGAGTGGTGGTTTATTCTTTGATTTAGATGACGAAGATGATTATA
AGAAATGTTTTAGTTTTTTAGAGCCATTCATTCAATTTAAAGAATCAAATTGTAACCCAA
AAGAATATTTTAATGGTACAATCATTCGTCTACCATTGNNNNNNNNNN
Length of 5' end seq. 588
3' end seq. ID VHE123Z
3' end seq. 
>VHE123Z.Seq
NNNNNNNNNNATTTTATACTCATAGCTTTCTTTACACGAGTGATCAAGTCTATGATCTCT
TATTCAAATATTATGGTAATAAGTTGGCCAAACATTTAAAATTTTCTACATTACCATTAA
ACTTTAGACAATTAATTTCATCAATTTGTAGAAATTATATAAATATTAGAGAAATTAAAC
TTTCAGATTTTATCACTAGTCTCAACTCCATCAACAACCTCCAATTTTTAAGTGATAGTA
TAATAACAATTTGGAATTGTATCGATGAATTAAAAAGAAAAGAAACTTTAGAATTATCTC
ATATCCAATCATTACAAATTATCCCATATAGATTAAGTCCAACTGAAACTGATGTCACTA
GTTTTGATAAATCTTCATTGTTAATGTACAAATCAAATTTCTTACCAATTGAACCAATCT
TAAACAAACTAAAAATCTATTTAATAAAAGAAGGACATCAAATCAATAGTTTATTAGATG
GAAATGCTCAAATCCCAATTTTATTAGGCTATCTTCAAAGGGTAAATTTATCATTAGACT
CAAAGGAAATCGATAAATTAAGAATATTTTTAGTTGAATCAATGTTCCAAATTTCTTTAT
CACAAGATTATACTTCAATTTTTTATAATTTACCAATTCACCCATGCATTGGAAATAGTA
ACCAAAAGTTTAGTAGTATTACAACCAGTAGTTACTGTGTGGATTAGAAAAAATGATCAA
TANAAATTCCAGGATTAAAATTAATAAAGGGTTAANAATTAACAAAA
Length of 3' end seq. 767
Connected seq. ID VHE123P
Connected seq. 
>VHE123P.Seq
AAAGAGATAAAATGCCCCAAAATAAAAGTCAAACAATAAAACTATCTCATTTTTTAAGAG
ATAATCTTCTTAACTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATGCAGAAG
ATGCTCGAGCTGATACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATAATGGGT
TGTTAACTTCAAATGATAACCAAGAAATTGAAAGTTCAAAAGAATTATTAGGTCCATCAA
TTTTAATTTATAATAATGCAACTTTTCAAGAACATGATTGGGAAGGAATTTGTCAAATTA
GTCAAGGTAGTAAAAAAGAAAATTTAAAAAGTGTTGGCAATTTCGGTTTAGGTTGGAACA
GTGTTTATCATATAACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGTTCTCAG
ATCCAAACGAAAGAATGAGTGGTGGTTTATTCTTTGATTTAGATGACGAAGATGATTATA
AGAAATGTTTTAGTTTTTTAGAGCCATTCATTCAATTTAAAGAATCAAATTGTAACCCAA
AAGAATATTTTAATGGTACAATCATTCGTCTACCATTG----------ATTTTATACTCA
TAGCTTTCTTTACACGAGTGATCAAGTCTATGATCTCTTATTCAAATATTATGGTAATAA
GTTGGCCAAACATTTAAAATTTTCTACATTACCATTAAACTTTAGACAATTAATTTCATC
AATTTGTAGAAATTATATAAATATTAGAGAAATTAAACTTTCAGATTTTATCACTAGTCT
CAACTCCATCAACAACCTCCAATTTTTAAGTGATAGTATAATAACAATTTGGAATTGTAT
CGATGAATTAAAAAGAAAAGAAACTTTAGAATTATCTCATATCCAATCATTACAAATTAT
CCCATATAGATTAAGTCCAACTGAAACTGATGTCACTAGTTTTGATAAATCTTCATTGTT
AATGTACAAATCAAATTTCTTACCAATTGAACCAATCTTAAACAAACTAAAAATCTATTT
AATAAAAGAAGGACATCAAATCAATAGTTTATTAGATGGAAATGCTCAAATCCCAATTTT
ATTAGGCTATCTTCAAAGGGTAAATTTATCATTAGACTCAAAGGAAATCGATAAATTAAG
AATATTTTTAGTTGAATCAATGTTCCAAATTTCTTTATCACAAGATTATACTTCAATTTT
TTATAATTTACCAATTCACCCATGCATTGGAAATAGTAACCAAAAGTTTAGTAGTATTAC
AACCAGTAGTTACTGTGTGGATTAGAAAAAATGATCAATANAAATTCCAGGATTAAAATT
AATAAAGGGTTAANAATTAACAAAA
Length of connected seq. 1335
Full length Seq ID -
Full length Seq. -
Length of full length seq. -