>VHE119 (VHE119Q) /CSM/VH/VHE1-A/VHE119Q.Seq.d/
AATTCAGTAGTTCAATTTAATTTATAATAATAATAATAGTATAAATATATAAAGATTAAT
TTTTTTGTATATAAATTTTTGTAAATAAATAAATAAATAATAATTTTTTTTTTAATTCAC
ACACATACATATAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTATTGAT
TTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTATTGCA
AATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAACGTTTA
ATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTGATGCT
AAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAACATTGG
CCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAATTCAAAGGT
GAAACTAAAGTTTXXXXXXXXXXGTGGTTCAACTCGTATTCCAAAGGTACAACAATTATT
ACAAGAATTTTTCAATGGTAAAGAATTGAATAAATCAATTAATCCAGATGAAGCTGTTGC
TTATGGTGCCGCTGTACAAGCTGCAATTCTTTCAAATGAAGGTGGTGCTAAAGTTGCTGA
TCTTTTATTGTTAGATGTTGCACCATTATCAATGGGTTTAGAAACTGCTGGTGGTGTTAT
GACAACTCTTATTCCAAGAAATACAACAATTCCATGTAAGAAAACTCAAACCTTCTCTAC
ATACTCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGAC
CAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGG
TGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGC
TGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCT
TTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGA
ACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAA
AAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTAT
G

nsvvqfnl*****ykyikinffvykfl*ink*iiifflihthtyky*iiky*KMSSIGID
LGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDA
KRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKGETKV---

---GSTRIPKVQQLLQEFFNGKELNKSINPDEAVAYGAAVQAAILSNEGGAKVADLLLLD
VAPLSMGLETAGGVMTTLIPRNTTIPCKKTQTFSTYSDNQPGVLIQVYEGERAMTKDNNL
LGKFELSGIPPAPRGVPQVEVTFDVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEI
EKMVADAEKFKQQDEQQKDRVESKNKLENYAFTVKNSIKDEKVAAKISDSDKSTM

Frame A:
nsvvqfnl*****ykyikinffvykfl*ink*iiifflihthtyky*iiky*KMSSIGID
LGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDA
KRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKGETKV---

---vvqlvfqrynnyyknfsmvkn*inqliqmklllmvplyklqffqmkvvlkllifyc*
mlhhyqwv*kllvvl*qllfqeiqqfhvrklkpslhtliinqvy*fkfmkvnvl*pkiii
ywvnlnflvfhqhqevyhklklhlmlmqmvs*myqlkinqlvtnkrlqlpmikvvfqkkk
lkkwllmqrnsnnkmnnkkivlnqrinwkimhsllkiqlkmkklqpkfqiqinql

Frame B:
iq*fnliynnnnsini*rliflyinfck*ink**fff*fthihinik*lnikkchqlvli
*vqhihvlvfgkmivlkllqmikvieplhlmlhlliqnv*lvmlqriklq*iqpiqylml
nv*lvvnlvtkkfkvi*nighsklfqrmvishifklnskvklkf---

---wfnsyskgttiitrifqw*rie*in*sr*scclwcrctscnsfk*rwc*sc*sfivr
cctiingfrncwwcydnsyskkynnsm*ensnllyil**strcinssl*r*tcydqr**f
ig*i*tfwyststkrctts*syi*c*ckwylkcis*r*inw*qtkdynyq**rssfkrrn
*kngc*creiqttr*ttkrsc*ike*igklcihc*kfn*r*kscsqnfrfr*iny

Frame C:
fsssi*fiiiiiv*iykd*ffci*ifvnk*innnfffnshtyi*ilnn*ilknvinwy*f
rynifmcwclak*sc*nyck*sr**nhsilcciy*yrtfnw*cckesscnesnqysi*c*
tfnws*i**qrssk*yetlaiqsyskgw**atyss*iqr*n*s---

---GSTRIPKVQQLLQEFFNGKELNKSINPDEAVAYGAAVQAAILSNEGGAKVADLLLLD
VAPLSMGLETAGGVMTTLIPRNTTIPCKKTQTFSTYSDNQPGVLIQVYEGERAMTKDNNL
LGKFELSGIPPAPRGVPQVEVTFDVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEI
EKMVADAEKFKQQDEQQKDRVESKNKLENYAFTVKNSIKDEKVAAKISDSDKSTM

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE119 (VHE119Q) /CSM/VH/VHE1-A/VHE119Q.Seq.d/                       2097   0.0
SHE551 (SHE551Q) /CSM/SH/SHE5-C/SHE551Q.Seq.d/                       1382   0.0
SFG805 (SFG805Q) /CSM/SF/SFG8-A/SFG805Q.Seq.d/                       1374   0.0
SFB318 (SFB318Q) /CSM/SF/SFB3-A/SFB318Q.Seq.d/                       1374   0.0
CHQ192 (CHQ192Q) /CSM/CH/CHQ1-D/CHQ192Q.Seq.d/                       1374   0.0
AFE717 (AFE717Q) /CSM/AF/AFE7-A/AFE717Q.Seq.d/                       1374   0.0
SFC313 (SFC313Q) /CSM/SF/SFC3-A/SFC313Q.Seq.d/                       1358   0.0
SFK589 (SFK589Q) /CSM/SF/SFK5-D/SFK589Q.Seq.d/                       1225   0.0
AHJ795 (AHJ795Q) /CSM/AH/AHJ7-D/AHJ795Q.Seq.d/                       1201   0.0
SFD523 (SFD523Q) /CSM/SF/SFD5-A/SFD523Q.Seq.d/                       1193   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1380   0.0    1
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1348   0.0    1
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   270   e-172  6
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   270   e-160  9
AF221541|AF221541.1 Cryptosporidium serpentis heat shock protein 70 (HSP70) gene, partial cds.   145   e-130  10
AY954892|AY954892.1 Cryptosporidium andersoni strain HenanZhzhca heat shock protein 70 gene, partial cds.   123   e-115  10
AY954893|AY954893.1 Cryptosporidium andersoni strain changchunca heat shock protein 70 gene, partial cds.   129   e-114  10
AY954894|AY954894.1 Cryptosporidium andersoni strain bjcm heat shock protein 70 gene, partial cds.   117   e-114  10
AF221543|AF221543.1 Cryptosporidium muris isolate 707 heat shock protein 70 (HSP70) gene, partial cds.   123   e-113  9
DQ060426|DQ060426.1 Cryptosporidium andersoni strain zzca heat shock protein 70-like gene, partial sequence.   127   e-111  9

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK303980_1(AK303980|pid:none) Homo sapiens cDNA FLJ54342 complet...   293   e-113
AK097824_1(AK097824|pid:none) Homo sapiens cDNA FLJ40505 fis, cl...   290   e-112
(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   379   e-103
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   379   e-103
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   339   1e-91
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   339   1e-91
FB790112_1(FB790112|pid:none) Sequence 9385 from Patent WO200803...   317   7e-85
FB790108_1(FB790108|pid:none) Sequence 9381 from Patent WO200803...   316   9e-85
AF033852_1(AF033852|pid:none) Spinacia oleracea cytosolic heat s...   316   9e-85
(P25840) RecName: Full=Heat shock 70 kDa protein;  &FB790406_1(F...   315   2e-84

 psg: 0.87  gvh: 0.35  alm: 0.39  top: 0.27  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHE119 is cyt

>VHE119F.Seq
AATTCAGTAGTTCAATTTAATTTATAATAATAATAATAGTATAAATATATAAAGATTAAT
TTTTTTGTATATAAATTTTTGTAAATAAATAAATAAATAATAATTTTTTTTTTAATTCAC
ACACATACATATAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTATTGAT
TTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTATTGCA
AATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAACGTTTA
ATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTGATGCT
AAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAACATTGG
CCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAATTCAAAGGT
GAAACTAAAGTTTNNNNNNNNNN

>VHE119Z.Seq
NNNNNNNNNNGTGGTTCAACTCGTATTCCAAAGGTACAACAATTATTACAAGAATTTTTC
AATGGTAAAGAATTGAATAAATCAATTAATCCAGATGAAGCTGTTGCTTATGGTGCCGCT
GTACAAGCTGCAATTCTTTCAAATGAAGGTGGTGCTAAAGTTGCTGATCTTTTATTGTTA
GATGTTGCACCATTATCAATGGGTTTAGAAACTGCTGGTGGTGTTATGACAACTCTTATT
CCAAGAAATACAACAATTCCATGTAAGAAAACTCAAACCTTCTCTACATACTCTGATAAT
CAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAAT
TTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTT
GAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCA
ACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAA
ATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGAT
CGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAA
GATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATG

>VHE119P.Seq
AATTCAGTAGTTCAATTTAATTTATAATAATAATAATAGTATAAATATATAAAGATTAAT
TTTTTTGTATATAAATTTTTGTAAATAAATAAATAAATAATAATTTTTTTTTTAATTCAC
ACACATACATATAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTATTGAT
TTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTATTGCA
AATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAACGTTTA
ATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTGATGCT
AAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAACATTGG
CCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAATTCAAAGGT
GAAACTAAAGTTT----------GTGGTTCAACTCGTATTCCAAAGGTACAACAATTATT
ACAAGAATTTTTCAATGGTAAAGAATTGAATAAATCAATTAATCCAGATGAAGCTGTTGC
TTATGGTGCCGCTGTACAAGCTGCAATTCTTTCAAATGAAGGTGGTGCTAAAGTTGCTGA
TCTTTTATTGTTAGATGTTGCACCATTATCAATGGGTTTAGAAACTGCTGGTGGTGTTAT
GACAACTCTTATTCCAAGAAATACAACAATTCCATGTAAGAAAACTCAAACCTTCTCTAC
ATACTCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGAC
CAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGG
TGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGC
TGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCT
TTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGA
ACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAA
AAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTAT
G