>VHE105 (VHE105Q) /CSM/VH/VHE1-A/VHE105Q.Seq.d/
XXXXXXXXXXGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAGTANA
AATTACTGCCCCANAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGG
TATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTT
ACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGC
TTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAANATAAGAA
AGCCACTGAAGTCGCTTTAGCCNCCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGA
TCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTNAGTTTTCAAACA
TAGGAATCAAATCTAAAAAAAATTTTAAAAAAAAAANA

---ELTASPTXLEPXYLVXITAPXNAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLP
VLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKXKKATEVALAXRXRKGLAPEIPDL
DKFHEKL*ti*valxvfkhrnqi*kkf*kkx

Frame A:
---ln*lppqlx*nqxt*xkllpxktllvvftlysivvevlllvknvvsvphyslskliy
qy*npsvspliyvptlpvklshnvssiiglqlvlstxirkplksl*ppexekv*pqksqi
ltnstknskqfkshfxfsnigikskknfkkkx


Frame B:
---*inclpnxxrtnxlsxnycpxkrywwyllctqss*rycyw*rtsyrfpiilcqssft
sirilrfhr*ftfphcrssfptmclrslgfnwccqqx*esh*srfsxqkxkrfsprnprs
*qiprktlnnlsrtxsfqt*esnlkkilkkk


Frame C:
---ELTASPTXLEPXYLVXITAPXNAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLP
VLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKXKKATEVALAXRXRKGLAPEIPDL
DKFHEKL*ti*valxvfkhrnqi*kkf*kkx

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP455 (VHP455Q) /CSM/VH/VHP4-C/VHP455Q.Seq.d/                        787   0.0
VHE105 (VHE105Q) /CSM/VH/VHE1-A/VHE105Q.Seq.d/                        787   0.0
VHQ463 (VHQ463Q) /CSM/VH/VHQ4-C/VHQ463Q.Seq.d/                        779   0.0
VHP196 (VHP196Q) /CSM/VH/VHP1-D/VHP196Q.Seq.d/                        779   0.0
VHO787 (VHO787Q) /CSM/VH/VHO7-D/VHO787Q.Seq.d/                        779   0.0
VHK530 (VHK530Q) /CSM/VH/VHK5-B/VHK530Q.Seq.d/                        779   0.0
VHQ104 (VHQ104Q) /CSM/VH/VHQ1-A/VHQ104Q.Seq.d/                        775   0.0
VHO438 (VHO438Q) /CSM/VH/VHO4-B/VHO438Q.Seq.d/                        775   0.0
VHK883 (VHK883Q) /CSM/VH/VHK8-D/VHK883Q.Seq.d/                        775   0.0
VHQ395 (VHQ395Q) /CSM/VH/VHQ3-D/VHQ395Q.Seq.d/                        773   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   636   0.0    2
EC760858|EC760858.1 PSE00006557 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.   113   1e-26  2
EC760375|EC760375.1 PSE00007370 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.   113   1e-26  2
EC762230|EC762230.1 PSE00004533 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.   113   3e-24  2
EH015431|EH015431.1 USDA-FP_188032 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt081_G08 5', mRNA sequence.    76   1e-14  2
EH012247|EH012247.1 USDA-FP_185167 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt044_H07 5', mRNA sequence.    76   1e-14  2
CK811111|CK811111.1 Rasgsc6510 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    84   8e-13  2
CK807987|CK807987.1 Rasgsc0236 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    84   8e-13  2
CK808524|CK808524.1 Rasgsc1215 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    84   9e-13  2
CK808261|CK808261.1 Rasgsc0738 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    84   9e-13  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q06193) RecName: Full=Elongation factor 2;          Short=EF-2;...   130   1e-29
AY831447_1(AY831447|pid:none) Monocercomonoides sp. PA203 transl...   126   3e-28
AB240142_1(AB240142|pid:none) Chlorella pyrenoidosa EF-2 mRNA fo...   124   1e-27
CP000881_15(CP000881|pid:none) Hemiselmis andersenii chromosome ...   123   2e-27
(P28996) RecName: Full=Elongation factor 2;          Short=EF-2;...   122   4e-27
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   121   9e-27
CR940347_690(CR940347|pid:none) Theileria annulata strain Ankara...   120   1e-26
A40411(A40411) translation elongation factor eEF-2 - Caenorhabdi...   120   1e-26
DQ848932_1(DQ848932|pid:none) Scophthalmus maximus clone kbc101 ...   120   2e-26
BC077595_1(BC077595|pid:none) Xenopus laevis translation elongat...   120   2e-26

 psg: 0.75  gvh: 0.53  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: peroxisomal
	  4.0 %: mitochondrial

>> prediction for VHE105 is cyt

>VHE105Z.Seq
NNNNNNNNNNGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAGTANA
AATTACTGCCCCANAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGG
TATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTT
ACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGC
TTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAANATAAGAA
AGCCACTGAAGTCGCTTTAGCCNCCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGA
TCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTNAGTTTTCAAACA
TAGGAATCAAATCTAAAAAAAATTTTAAAAAAAAAANA