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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHD861
Library VH
(Link to library)
Clone ID VHD861
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14941-1
Original site URL
Representative seq. ID VHD861P
(Link to Original site)
Representative DNA sequence 
>VHD861 (VHD861Q) /CSM/VH/VHD8-C/VHD861Q.Seq.d/
ATTTTTATTTTTTTATTTTTTTTTAATATAAATACTTTTATATATACATTAAAAGAAATG
TCAACAGAAGAAGATCAAAAATTATTCCTTTCAATTGGATTAGATGAAAAGAGAGCAAAA
GATACTTTAAAAAATAAAGATCTTACTGCATTATTAAAAACCATTATTGAAGAAGCATCC
GTTTCAAAAGGTTGTGATAAATCAATTGGTAACCTTTTATATAGTTTAGCAACTTGTCAA
CAAGTAAACTCACCAATTCGTTCAAACGTTTCAAAAGCAATTGGTGAAGAAAAAATTAAA
ACCACCATTCAATTCCAAGCTGCAACTCAATACTTAAAAGATAACAAAGAATTCAATCAA
GATACATTTAACGATTTTTGTGGTGTTGGTGTTGTCATCACACCAGAACAAATTCAAAAA
GCTATTGATGATTTATTCACTAAAAAAGCTACTGAAATTTCTGAAAAAAAATGGCATATA
CCAATTGGTGATTTATTAAATCCATTAAAAGAATCATTAAAATGGGCAGATTTAAAAGAA
GTTAAAATAATGTTAGATAAAAAATTAGAGACAACATTAGGAXXXXXXXXXXACGAGNAA
CTTCAAGATCGTATCGAATTCTTGGTTGTCGATGTTTGTGAAAAACTCTTCAAAATGGAC
CCAGAATTGATCCTCTCTGTCAATCCAGACTTTAAAGTACCAAAGAGACCATTCATGAGA
ATGAACTATTCCGATGCTATCGAATATTGTAAAAAGAATGGTATCCAAAAGAAACTTGAA
GATGGTACTGAAGTCGATTTCGAATTTGGTGATGATATTCCAGAGGCTCAAGAAAGAAGA
ATGAATGATCAAATCGGTGAACCAATCTTCCTCTGTAGATTCCCTGCTGAAATGAAAGCT
TTCTACATGGCTAGATGTCCAGAAGATAGAACTCTCACTGAATCTCTTGATCTCTTAATG
CCAGGTGTTGGTGAAATCGTTGGTGGTTCTATGAGAATTTCAGATTTCAATGAATTGGTT
GCAGCTTATAAAAAAGCTGGTCTTGATACTGATGAATATTATTGGTTCACTGATCAACGT
AAATATGGTACTACTCAACATGGTGGTTATGGTTTAGGTGTTGANCGTTTCCTTACTTGG
ATGCTCAAAGAAGAACATATTCGTAATGTTTGTGTTTACCAACGTACAAAAGATAGAATT
ACCCCATAAAATATTTTTTGATTTTAATTTCTCAATTCTAAAATCATTTTGATCAAATAA
A
sequence update 2002.10.25
Translated Amino Acid sequence 
IFIFLFFFNINTFIYTLKEMSTEEDQKLFLSIGLDEKRAKDTLKNKDLTALLKTIIEEAS
VSKGCDKSIGNLLYSLATCQQVNSPIRSNVSKAIGEEKIKTTIQFQAATQYLKDNKEFNQ
DTFNDFCGVGVVITPEQIQKAIDDLFTKKATEISEKKWHIPIGDLLNPLKESLKWADLKE
VKIMLDKKLETTLG---

---EXLQDRIEFLVVDVCEKLFKMDPELILSVNPDFKVPKRPFMRMNYSDAIEYCKKNGI
QKKLEDGTEVDFEFGDDIPEAQERRMNDQIGEPIFLCRFPAEMKAFYMARCPEDRTLTES
LDLLMPGVGEIVGGSMRISDFNELVAAYKKAGLDTDEYYWFTDQRKYGTTQHGGYGLGVX
RFLTWMLKEEHIRNVCVYQRTKDRITP*nif*f*flnskiilik*
Translated Amino Acid sequence (All Frames) 
Frame A:
IFIFLFFFNINTFIYTLKEMSTEEDQKLFLSIGLDEKRAKDTLKNKDLTALLKTIIEEAS
VSKGCDKSIGNLLYSLATCQQVNSPIRSNVSKAIGEEKIKTTIQFQAATQYLKDNKEFNQ
DTFNDFCGVGVVITPEQIQKAIDDLFTKKATEISEKKWHIPIGDLLNPLKESLKWADLKE
VKIMLDKKLETTLG---

---txnfkivsnswlsmfvknsskwtqn*sslsiqtlkyqrdhs*e*tipmlsnivkrmv
skrnlkmvlksisnlvmifqrlkkee*miksvnqsssvdsllk*klstwldvqkielsln
llis*cqvlvkslvvl*efqismnwlqlikklvlilmniigslinvnmvllnmvvmv*vl
xvsllgcskknifvmfvftnvqkielphkiffdfnfsilksf*snk

Frame B:
flffyffli*illyih*kkcqqkkiknysfqld*mkreqkil*kikillhy*kpllkkhp
fqkvvinqlvtfyiv*qlvnk*thqfvqtfqkqlvkkklkppfnsklqlnt*kitknsik
ihltifvvlvlsshqnkfkkllmiyslkkllkflkkngiyqlviy*ih*knh*ngqi*kk
lk*c*ikn*rqh*---

---rxtsrsyrilgcrcl*ktlqngpridplcqsrl*stketihenelfrcyril*kewy
pket*rwy*srfriw**ysrgsrkkne*snr*tnlpl*ipc*nesflhg*msrr*nsh*i
s*slnarcw*nrwwfyenfrfq*igcsl*ksws*y**illvh*st*iwyystwwlwfrc*
xfpyldaqrrtys*clclptykr*nypikyflilisqf*nhfdqi

Frame C:
fyffiff*ykyfyiyikrnvnrrrskiipfnwir*keskryfkk*rsyciiknhy*rsir
fkrl**inw*pfi*fsnlstskltnsfkrfksnw*rkn*nhhsipscnsilkr*qriqsr
yi*rflwcwcchhtrtnsksy**fih*ksy*nf*kkmaytnw*fiksikriikmgrfkrs
*nnvr*kirdnir---

---EXLQDRIEFLVVDVCEKLFKMDPELILSVNPDFKVPKRPFMRMNYSDAIEYCKKNGI
QKKLEDGTEVDFEFGDDIPEAQERRMNDQIGEPIFLCRFPAEMKAFYMARCPEDRTLTES
LDLLMPGVGEIVGGSMRISDFNELVAAYKKAGLDTDEYYWFTDQRKYGTTQHGGYGLGVX
RFLTWMLKEEHIRNVCVYQRTKDRITP*nif*f*flnskiilik*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD861 (VHD861Q) /CSM/VH/VHD8-C/VHD861Q.Seq.d/                       2333   0.0
VHO452 (VHO452Q) /CSM/VH/VHO4-C/VHO452Q.Seq.d/                       1314   0.0
VHI167 (VHI167Q) /CSM/VH/VHI1-C/VHI167Q.Seq.d/                       1314   0.0
VHC801 (VHC801Q) /CSM/VH/VHC8-A/VHC801Q.Seq.d/                       1300   0.0
VHC634 (VHC634Q) /CSM/VH/VHC6-B/VHC634Q.Seq.d/                       1300   0.0
SFE837 (SFE837Q) /CSM/SF/SFE8-B/SFE837Q.Seq.d/                       1300   0.0
SLE204 (SLE204Q) /CSM/SL/SLE2-A/SLE204Q.Seq.d/                       1287   0.0
SFF879 (SFF879Q) /CSM/SF/SFF8-D/SFF879Q.Seq.d/                       1285   0.0
VHB335 (VHB335Q) /CSM/VH/VHB3-B/VHB335Q.Seq.d/                       1283   0.0
SFK826 (SFK826Q) /CSM/SF/SFK8-B/SFK826Q.Seq.d/                       1281   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.  1031   0.0    7
EE004071|EE004071.1 ROE00007211 Rhizopus oryzae Home made (1-5 kb) Glucose Rhizopus oryzae cDNA, mRNA sequence.    92   5e-14  1
ES217666|ES217666.1 MpFVN_ag1_H24 Myzus persicae, line F001, PLRV free, whole female aphid library Myzus persicae cDNA clone MpFVN_ag1_H24, mRNA sequence.    90   2e-13  1
AM643549|AM643549.1 Entamoeba dispar GSS, clone dispar85b10.p1k.    84   1e-11  1
CX089960|CX089960.1 EHAE646TR E. histolytica Normalized cDNA library Entamoeba histolytica cDNA, mRNA sequence.    80   2e-10  1
CX081759|CX081759.1 EHAAM58TR E. histolytica Normalized cDNA library Entamoeba histolytica cDNA, mRNA sequence.    80   2e-10  1
AZ692581|AZ692581.1 ENTGY45TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   2e-10  1
CN761262|CN761262.1 ID0AAA2DD07RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA2DD07 5', mRNA sequence.    64   4e-06  2
CN756331|CN756331.1 ID0AAA18BF03RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA18BF03 5', mRNA sequence.    64   1e-05  1
CN583980|CN583980.1 USDA-FP_127046 Acyrthosiphon pisum, Pea Aphid Acyrthosiphon pisum cDNA clone WHAP053_C04, mRNA sequence.    58   6e-04  1
dna update 2007. 6.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116305_93(AC116305|pid:none) Dictyostelium discoideum chromoso...   419   e-115
(Q4R4Z1) RecName: Full=Asparaginyl-tRNA synthetase, cytoplasmic;...   249   1e-64
(O43776) RecName: Full=Asparaginyl-tRNA synthetase, cytoplasmic;...   248   2e-64
AK297777_1(AK297777|pid:none) Homo sapiens cDNA FLJ52703 complet...   248   2e-64
EF215197_1(EF215197|pid:none) Callithrix jacchus clone D12-40.4_...   248   4e-64
AK222835_1(AK222835|pid:none) Homo sapiens mRNA for asparaginyl-...   247   7e-64
(Q8BP47) RecName: Full=Asparaginyl-tRNA synthetase, cytoplasmic;...   246   9e-64
AK077699_1(AK077699|pid:none) Mus musculus 8 days embryo whole b...   246   9e-64
AY387099_1(AY387099|pid:none) Rattus norvegicus LRRGT00113 mRNA,...   246   2e-63
BC099099_1(BC099099|pid:none) Rattus norvegicus asparaginyl-tRNA...   246   2e-63
protein update 2009. 6.30
PSORT 

 psg: 1.04  gvh: 0.40  alm: 0.30  top: 0.33  tms: 0.07  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 16.0 %: mitochondrial
	 16.0 %: nuclear
	  8.0 %: plasma membrane
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall

>> prediction for VHD861 is end
5' end seq. ID VHD861F
5' end seq. 
>VHD861F.Seq
ATTTTTATTTTTTTATTTTTTTTTAATATAAATACTTTTATATATACATTAAAAGAAATG
TCAACAGAAGAAGATCAAAAATTATTCCTTTCAATTGGATTAGATGAAAAGAGAGCAAAA
GATACTTTAAAAAATAAAGATCTTACTGCATTATTAAAAACCATTATTGAAGAAGCATCC
GTTTCAAAAGGTTGTGATAAATCAATTGGTAACCTTTTATATAGTTTAGCAACTTGTCAA
CAAGTAAACTCACCAATTCGTTCAAACGTTTCAAAAGCAATTGGTGAAGAAAAAATTAAA
ACCACCATTCAATTCCAAGCTGCAACTCAATACTTAAAAGATAACAAAGAATTCAATCAA
GATACATTTAACGATTTTTGTGGTGTTGGTGTTGTCATCACACCAGAACAAATTCAAAAA
GCTATTGATGATTTATTCACTAAAAAAGCTACTGAAATTTCTGAAAAAAAATGGCATATA
CCAATTGGTGATTTATTAAATCCATTAAAAGAATCATTAAAATGGGCAGATTTAAAAGAA
GTTAAAATAATGTTAGATAAAAAATTAGAGACAACATTAGGANNNNNNNNNN
Length of 5' end seq. 592
3' end seq. ID VHD861Z
3' end seq. 
>VHD861Z.Seq
NNNNNNNNNNACGAGNAACTTCAAGATCGTATCGAATTCTTGGTTGTCGATGTTTGTGAA
AAACTCTTCAAAATGGACCCAGAATTGATCCTCTCTGTCAATCCAGACTTTAAAGTACCA
AAGAGACCATTCATGAGAATGAACTATTCCGATGCTATCGAATATTGTAAAAAGAATGGT
ATCCAAAAGAAACTTGAAGATGGTACTGAAGTCGATTTCGAATTTGGTGATGATATTCCA
GAGGCTCAAGAAAGAAGAATGAATGATCAAATCGGTGAACCAATCTTCCTCTGTAGATTC
CCTGCTGAAATGAAAGCTTTCTACATGGCTAGATGTCCAGAAGATAGAACTCTCACTGAA
TCTCTTGATCTCTTAATGCCAGGTGTTGGTGAAATCGTTGGTGGTTCTATGAGAATTTCA
GATTTCAATGAATTGGTTGCAGCTTATAAAAAAGCTGGTCTTGATACTGATGAATATTAT
TGGTTCACTGATCAACGTAAATATGGTACTACTCAACATGGTGGTTATGGTTTAGGTGTT
GANCGTTTCCTTACTTGGATGCTCAAAGAAGAACATATTCGTAATGTTTGTGTTTACCAA
CGTACAAAAGATAGAATTACCCCATAAAATATTTTTTGATTTTAATTTCTCAATTCTAAA
ATCATTTTGATCAAATAAA
Length of 3' end seq. 679
Connected seq. ID VHD861P
Connected seq. 
>VHD861P.Seq
ATTTTTATTTTTTTATTTTTTTTTAATATAAATACTTTTATATATACATTAAAAGAAATG
TCAACAGAAGAAGATCAAAAATTATTCCTTTCAATTGGATTAGATGAAAAGAGAGCAAAA
GATACTTTAAAAAATAAAGATCTTACTGCATTATTAAAAACCATTATTGAAGAAGCATCC
GTTTCAAAAGGTTGTGATAAATCAATTGGTAACCTTTTATATAGTTTAGCAACTTGTCAA
CAAGTAAACTCACCAATTCGTTCAAACGTTTCAAAAGCAATTGGTGAAGAAAAAATTAAA
ACCACCATTCAATTCCAAGCTGCAACTCAATACTTAAAAGATAACAAAGAATTCAATCAA
GATACATTTAACGATTTTTGTGGTGTTGGTGTTGTCATCACACCAGAACAAATTCAAAAA
GCTATTGATGATTTATTCACTAAAAAAGCTACTGAAATTTCTGAAAAAAAATGGCATATA
CCAATTGGTGATTTATTAAATCCATTAAAAGAATCATTAAAATGGGCAGATTTAAAAGAA
GTTAAAATAATGTTAGATAAAAAATTAGAGACAACATTAGGA----------ACGAGNAA
CTTCAAGATCGTATCGAATTCTTGGTTGTCGATGTTTGTGAAAAACTCTTCAAAATGGAC
CCAGAATTGATCCTCTCTGTCAATCCAGACTTTAAAGTACCAAAGAGACCATTCATGAGA
ATGAACTATTCCGATGCTATCGAATATTGTAAAAAGAATGGTATCCAAAAGAAACTTGAA
GATGGTACTGAAGTCGATTTCGAATTTGGTGATGATATTCCAGAGGCTCAAGAAAGAAGA
ATGAATGATCAAATCGGTGAACCAATCTTCCTCTGTAGATTCCCTGCTGAAATGAAAGCT
TTCTACATGGCTAGATGTCCAGAAGATAGAACTCTCACTGAATCTCTTGATCTCTTAATG
CCAGGTGTTGGTGAAATCGTTGGTGGTTCTATGAGAATTTCAGATTTCAATGAATTGGTT
GCAGCTTATAAAAAAGCTGGTCTTGATACTGATGAATATTATTGGTTCACTGATCAACGT
AAATATGGTACTACTCAACATGGTGGTTATGGTTTAGGTGTTGANCGTTTCCTTACTTGG
ATGCTCAAAGAAGAACATATTCGTAATGTTTGTGTTTACCAACGTACAAAAGATAGAATT
ACCCCATAAAATATTTTTTGATTTTAATTTCTCAATTCTAAAATCATTTTGATCAAATAA
A
Length of connected seq. 1251
Full length Seq ID -
Full length Seq. -
Length of full length seq. -