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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHD728
Library VH
(Link to library)
Clone ID VHD728
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U12327-1
Original site URL
Representative seq. ID VHD728P
(Link to Original site)
Representative DNA sequence 
>VHD728 (VHD728Q) /CSM/VH/VHD7-B/VHD728Q.Seq.d/
GCTTCATTAAAGAAATTCACTCATCAATCGGAACATTACTCTTTAAGTGGTGGTATTTTC
GTTGAAAACTCAAGTTTACTTCAAAATGAAAAGGCTCCAATCGTTGTAAGAGTTAAATTG
TTCTTGGGTGATACTCAAATCTCAAACAATTATCTCGAGGAACCATCTCTCACCATTACA
AGTTCTGATAATTCTGAATCACCAGTTGTAAACTCAAAGGAAATTAAACCATTTGCTCTA
TTCGATAATAAAGAATCTGTATATCAATATAAAGTTCAAGAAGGTTTAAATAGTTTGACT
GTTCGTTTCGAAGCTAAAGTTCGTGCTCTTAGTAGAAATAATAATCAAGAGACTCTTTCA
TTTGAATCCACTTTCCAAGTTAATACTATCAATCAAACTGATCAAATCTCTGAATTCTAT
TTAGAACGTTGTCTAGCTAAAGATGGGTGTTCATTAGGTTATCAACTTTGTCATCTTGGT
AAAGGTGGTGATCCATTCTCTCATTTGGATGTCTCAATGGATTTCCAACATTGGTTAACC
TCTGAAAGAATTTATACCACTTTGAAAACCGATAAAAAXXXXXXXXXXGNAATATTTATC
AAAGAACAATTTGTATCGTGTTGATTTCTATCATCTCTATCATAGATATACCGATAAGAA
AGTTTGGGATAAAGTAATTGAACTCTTAAAGAAATTGAAATTCTATGAATCTATCACTTG
GTCATTCTCTATTCATCATAAGAATTTCGTTTACTTGAAAGATTACCTCTCTGAACAAAT
TCATTCCTTATCAATGAAATCAATCGATGCACCATCCATTTATGTTGATCCATTTGAAAA
TAACTTCATCAAATTCTTGGAATACTCTCCATTGGTTAATTCAAGAACTCATCAAATTGG
TGATGAAAGAAAGATTCTTAATAATAAATTATCAAATCAATATTATGAATTCTGTAGTTT
ACTTTCATTGAAATTAAATCCATCCGATACTGAATTACTTTCTTTAGCTTATTATTTATT
ACTTCAAGATAGATTTGATGAATCAATTAAAATTATGAAGAGAATTGGTAAACATCCAGT
TTCAATTCCAAAATTAATTTCTTCTTCAACCACTACTGATTCAAAATCAACTCATTCATC
AGTAATTTCTTCTTCTTCCTCTTCAAATTTATCAACCACTACTGATAGTTCAAAAGATAA
AAAGAAACTGAAAAAGAAGAAAAACAAAGAGAAAAAGAAAGAAAACAAAAAGAAAAAGAA
GNATAAAAAGNGNNNAAAAAGAAGA
sequence update 2002.10.25
Translated Amino Acid sequence 
ASLKKFTHQSEHYSLSGGIFVENSSLLQNEKAPIVVRVKLFLGDTQISNNYLEEPSLTIT
SSDNSESPVVNSKEIKPFALFDNKESVYQYKVQEGLNSLTVRFEAKVRALSRNNNQETLS
FESTFQVNTINQTDQISEFYLERCLAKDGCSLGYQLCHLGKGGDPFSHLDVSMDFQHWLT
SERIYTTLKTDK---

---XYLSKNNLYRVDFYHLYHRYTDKKVWDKVIELLKKLKFYESITWSFSIHHKNFVYLK
DYLSEQIHSLSMKSIDAPSIYVDPFENNFIKFLEYSPLVNSRTHQIGDERKILNNKLSNQ
YYEFCSLLSLKLNPSDTELLSLAYYLLLQDRFDESIKIMKRIGKHPVSIPKLISSSTTTD
SKSTHSSVISSSSSSNLSTTTDSSKDKKKLKKKKNKEKKKENKKKKKXKKXXKRR
Translated Amino Acid sequence (All Frames) 
Frame A:
ASLKKFTHQSEHYSLSGGIFVENSSLLQNEKAPIVVRVKLFLGDTQISNNYLEEPSLTIT
SSDNSESPVVNSKEIKPFALFDNKESVYQYKVQEGLNSLTVRFEAKVRALSRNNNQETLS
FESTFQVNTINQTDQISEFYLERCLAKDGCSLGYQLCHLGKGGDPFSHLDVSMDFQHWLT
SERIYTTLKTDK---

---xifikeqfvsc*flssls*iyr*eslg*sn*tlkeieil*iyhlvilyss*efrlle
rlpl*tnsflineinrctihlc*si*k*lhqilgilsig*fknssnw**kkds***iiks
il*il*ftfieiksiry*itffsllfitsr*i**in*nyeenw*tssfnskinfffnhy*
fkinsfisnfffflfkfinhy**fkr*ketekeekqrekerkqkekex*kxxkkk

Frame B:
lh*rnslinrnitl*vvvfslktqvyfkmkrlqsl*elncswvilksqtiisrnhlsplq
vliilnhql*tqrklnhllysiiknlyinikfkkv*iv*lfvsklkfvllveiiikrlfh
lnplsklilsiklikslnsi*nvv*lkmgvh*vinfvilvkvvihsliwmsqwisnig*p
lkefipl*kpik---

---XYLSKNNLYRVDFYHLYHRYTDKKVWDKVIELLKKLKFYESITWSFSIHHKNFVYLK
DYLSEQIHSLSMKSIDAPSIYVDPFENNFIKFLEYSPLVNSRTHQIGDERKILNNKLSNQ
YYEFCSLLSLKLNPSDTELLSLAYYLLLQDRFDESIKIMKRIGKHPVSIPKLISSSTTTD
SKSTHSSVISSSSSSNLSTTTDSSKDKKKLKKKKNKEKKKENKKKKKXKKXXKRR

Frame C:
fikeihssigtllfkwwyfr*klkftsk*kgsnrcks*ivlg*ysnlkqlsrgtishhyk
f**f*itscklkgn*ticsir**ricisi*ssrrfk*fdcsfrs*sscs**k**srdsfi
*ihfps*yyqsn*snl*ilfrtlss*rwvfirlstlssw*rw*silsfgclngfptlvnl
*knlyhfenr*k---

---niyqrticivlisiisiidipirkfgik*lns*rn*nsmnlslghslfiirisft*k
itslnkfipyq*nqsmhhpfmlihlkitssnswntlhwliqeliklvmkerfliinyqin
imnsvvyfh*n*ihpilnyfl*liiyyfkidlmnqlkl*relvniqfqfqn*fllqplli
qnqlihq*flllplqiyqpllivqkikrn*krrktkrkrkktkrkrxikxxxke
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD728 (VHD728Q) /CSM/VH/VHD7-B/VHD728Q.Seq.d/                       2296   0.0
VHP670 (VHP670Q) /CSM/VH/VHP6-C/VHP670Q.Seq.d/                       1191   0.0
VHP284 (VHP284Q) /CSM/VH/VHP2-D/VHP284Q.Seq.d/                       1191   0.0
VHN485 (VHN485Q) /CSM/VH/VHN4-D/VHN485Q.Seq.d/                       1191   0.0
VHC649 (VHC649Q) /CSM/VH/VHC6-C/VHC649Q.Seq.d/                       1191   0.0
VHC626 (VHC626Q) /CSM/VH/VHC6-B/VHC626Q.Seq.d/                       1191   0.0
VHB392 (VHB392Q) /CSM/VH/VHB3-D/VHB392Q.Seq.d/                       1191   0.0
VHH672 (VHH672Q) /CSM/VH/VHH6-C/VHH672Q.Seq.d/                       1183   0.0
VHA602 (VHA602Q) /CSM/VH/VHA6-A/VHA602Q.Seq.d/                       1183   0.0
VHN858 (VHN858Q) /CSM/VH/VHN8-C/VHN858Q.Seq.d/                       1179   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BG226304|BG226304.1 kq20a02.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5', mRNAsequence.    44   0.009  2
ES222874|ES222874.1 MpHnorm_ag1_G13 Myzus persicae, tobacco lineage, aphid head normalized library Myzus persicae cDNA clone MpHnorm_ag1_G13, mRNA sequence.    52   0.041  1
ES220333|ES220333.1 MpGnorm_ag6_F06 Myzus persicae, tobacco lineage, aphid digestive tract normalized library Myzus persicae cDNA clone MpGnorm_ag6_F06, mRNA sequence.    52   0.041  1
ES217936|ES217936.1 MpFVN_ag2_E24 Myzus persicae, line F001, PLRV free, whole female aphid library Myzus persicae cDNA clone MpFVN_ag2_E24, mRNA sequence.    52   0.041  1
ES450291|ES450291.1 25260 Myzus persicae 2001-12 (red), Fenton Myzus persicae cDNA clone GMAWCDNA24_F1_F01_003 5', mRNA sequence.    52   0.041  1
ES222874|ES222874.1 MpHnorm_ag1_G13 Myzus persicae, tobacco lineage, aphid head normalized library Myzus persicae cDNA clone MpHnorm_ag1_G13, mRNA sequence.    52   0.041  1
ES220333|ES220333.1 MpGnorm_ag6_F06 Myzus persicae, tobacco lineage, aphid digestive tract normalized library Myzus persicae cDNA clone MpGnorm_ag6_F06, mRNA sequence.    52   0.041  1
ES217936|ES217936.1 MpFVN_ag2_E24 Myzus persicae, line F001, PLRV free, whole female aphid library Myzus persicae cDNA clone MpFVN_ag2_E24, mRNA sequence.    52   0.041  1
DW014230|DW014230.1 w7l15_M13F Myzus persicae, tobacco lineage, whole aphid library Myzus persicae cDNA clone w7l15_M13F, mRNA sequence.    52   0.041  1
DW013855|DW013855.1 w6j1_M13F Myzus persicae, tobacco lineage, whole aphid library Myzus persicae cDNA clone w6j1_M13F, mRNA sequence.    52   0.041  1
dna update 2007. 6.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000551_1069(CP000551|pid:none) Prochlorococcus marinus str. AS...    38   0.90
AL844507_40(AL844507|pid:none) Plasmodium falciparum 3D7 chromos...    38   0.90
CP000685_1285(CP000685|pid:none) Flavobacterium johnsoniae UW101...    36   2.6
CP000111_1067(CP000111|pid:none) Prochlorococcus marinus str. MI...    35   4.4
AY088864_1(AY088864|pid:none) Arabidopsis thaliana clone 97569 m...    35   5.8
AY740026_3(AY740026|pid:none) Saccharomyces kudriavzevii IFO 180...    35   5.8
CP000409_33(CP000409|pid:none) Rickettsia canadensis str. McKiel...    35   5.8
(O54982) RecName: Full=Potassium channel subfamily U member 1; A...    35   7.6
T42383(T42383)probable calcium-activated potassium channel Slo3 ...    35   7.6
FN357525_10(FN357525|pid:none) Schistosoma mansoni genome sequen...    34   9.9
protein update 2009. 6.29
PSORT 

 psg: 0.91  gvh: 0.36  alm: 0.47  top: 0.27  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.65  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	  8.0 %: plasma membrane
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: cytoplasmic

>> prediction for VHD728 is nuc
5' end seq. ID VHD728F
5' end seq. 
>VHD728F.Seq
GCTTCATTAAAGAAATTCACTCATCAATCGGAACATTACTCTTTAAGTGGTGGTATTTTC
GTTGAAAACTCAAGTTTACTTCAAAATGAAAAGGCTCCAATCGTTGTAAGAGTTAAATTG
TTCTTGGGTGATACTCAAATCTCAAACAATTATCTCGAGGAACCATCTCTCACCATTACA
AGTTCTGATAATTCTGAATCACCAGTTGTAAACTCAAAGGAAATTAAACCATTTGCTCTA
TTCGATAATAAAGAATCTGTATATCAATATAAAGTTCAAGAAGGTTTAAATAGTTTGACT
GTTCGTTTCGAAGCTAAAGTTCGTGCTCTTAGTAGAAATAATAATCAAGAGACTCTTTCA
TTTGAATCCACTTTCCAAGTTAATACTATCAATCAAACTGATCAAATCTCTGAATTCTAT
TTAGAACGTTGTCTAGCTAAAGATGGGTGTTCATTAGGTTATCAACTTTGTCATCTTGGT
AAAGGTGGTGATCCATTCTCTCATTTGGATGTCTCAATGGATTTCCAACATTGGTTAACC
TCTGAAAGAATTTATACCACTTTGAAAACCGATAAAAANNNNNNNNNN
Length of 5' end seq. 588
3' end seq. ID VHD728Z
3' end seq. 
>VHD728Z.Seq
NNNNNNNNNNGNAATATTTATCAAAGAACAATTTGTATCGTGTTGATTTCTATCATCTCT
ATCATAGATATACCGATAAGAAAGTTTGGGATAAAGTAATTGAACTCTTAAAGAAATTGA
AATTCTATGAATCTATCACTTGGTCATTCTCTATTCATCATAAGAATTTCGTTTACTTGA
AAGATTACCTCTCTGAACAAATTCATTCCTTATCAATGAAATCAATCGATGCACCATCCA
TTTATGTTGATCCATTTGAAAATAACTTCATCAAATTCTTGGAATACTCTCCATTGGTTA
ATTCAAGAACTCATCAAATTGGTGATGAAAGAAAGATTCTTAATAATAAATTATCAAATC
AATATTATGAATTCTGTAGTTTACTTTCATTGAAATTAAATCCATCCGATACTGAATTAC
TTTCTTTAGCTTATTATTTATTACTTCAAGATAGATTTGATGAATCAATTAAAATTATGA
AGAGAATTGGTAAACATCCAGTTTCAATTCCAAAATTAATTTCTTCTTCAACCACTACTG
ATTCAAAATCAACTCATTCATCAGTAATTTCTTCTTCTTCCTCTTCAAATTTATCAACCA
CTACTGATAGTTCAAAAGATAAAAAGAAACTGAAAAAGAAGAAAAACAAAGAGAAAAAGA
AAGAAAACAAAAAGAAAAAGAAGNATAAAAAGNGNNNAAAAAGAAGA
Length of 3' end seq. 707
Connected seq. ID VHD728P
Connected seq. 
>VHD728P.Seq
GCTTCATTAAAGAAATTCACTCATCAATCGGAACATTACTCTTTAAGTGGTGGTATTTTC
GTTGAAAACTCAAGTTTACTTCAAAATGAAAAGGCTCCAATCGTTGTAAGAGTTAAATTG
TTCTTGGGTGATACTCAAATCTCAAACAATTATCTCGAGGAACCATCTCTCACCATTACA
AGTTCTGATAATTCTGAATCACCAGTTGTAAACTCAAAGGAAATTAAACCATTTGCTCTA
TTCGATAATAAAGAATCTGTATATCAATATAAAGTTCAAGAAGGTTTAAATAGTTTGACT
GTTCGTTTCGAAGCTAAAGTTCGTGCTCTTAGTAGAAATAATAATCAAGAGACTCTTTCA
TTTGAATCCACTTTCCAAGTTAATACTATCAATCAAACTGATCAAATCTCTGAATTCTAT
TTAGAACGTTGTCTAGCTAAAGATGGGTGTTCATTAGGTTATCAACTTTGTCATCTTGGT
AAAGGTGGTGATCCATTCTCTCATTTGGATGTCTCAATGGATTTCCAACATTGGTTAACC
TCTGAAAGAATTTATACCACTTTGAAAACCGATAAAAA----------GNAATATTTATC
AAAGAACAATTTGTATCGTGTTGATTTCTATCATCTCTATCATAGATATACCGATAAGAA
AGTTTGGGATAAAGTAATTGAACTCTTAAAGAAATTGAAATTCTATGAATCTATCACTTG
GTCATTCTCTATTCATCATAAGAATTTCGTTTACTTGAAAGATTACCTCTCTGAACAAAT
TCATTCCTTATCAATGAAATCAATCGATGCACCATCCATTTATGTTGATCCATTTGAAAA
TAACTTCATCAAATTCTTGGAATACTCTCCATTGGTTAATTCAAGAACTCATCAAATTGG
TGATGAAAGAAAGATTCTTAATAATAAATTATCAAATCAATATTATGAATTCTGTAGTTT
ACTTTCATTGAAATTAAATCCATCCGATACTGAATTACTTTCTTTAGCTTATTATTTATT
ACTTCAAGATAGATTTGATGAATCAATTAAAATTATGAAGAGAATTGGTAAACATCCAGT
TTCAATTCCAAAATTAATTTCTTCTTCAACCACTACTGATTCAAAATCAACTCATTCATC
AGTAATTTCTTCTTCTTCCTCTTCAAATTTATCAACCACTACTGATAGTTCAAAAGATAA
AAAGAAACTGAAAAAGAAGAAAAACAAAGAGAAAAAGAAAGAAAACAAAAAGAAAAAGAA
GNATAAAAAGNGNNNAAAAAGAAGA
Length of connected seq. 1275
Full length Seq ID -
Full length Seq. -
Length of full length seq. -