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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFN656
Library AF
(Link to library)
Clone ID AFN656
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig -
Original site URL
Representative seq. ID AFN656P
(Link to Original site)
Representative DNA sequence 
>AFN656 (AFN656Q) /CSM/AF/AFN6-C/AFN656Q.Seq.d/
AAAATTTTTTTTTTTATATNGTATTTTTAACACACCAAATATATATTTATATTTATATAT
TTATATATTTTTTTTTATTTTATTTTCACACCCTATTCTATATAAATTAATTTTTTTTTT
TTTTTANCNCCAATTTAAAAAAAAAAXXXXXXXXXXANAAAAGAAAATTATTAAAGTTAC
ATNTGAAGGTGAAACTGTAACATGTGATAAAATTGTTTGTGATCCATCATATTTCCCAAA
TAAAGTTCAAAANAAAGGTAAAATTATTCGTTCAATTTGTATTTTGAATGGTCCAATTCC
ATCTGNTGATAANAAATTTGAATCACTCCAAATCATTATCCCNCAAAAACAAGTTAAACG
TCAATCANATATGNACGTTGGTGTTATGTCATCCGCTCATTGTGTNTGTCCAAAGGGTAA
ATACATTGCCATCGTTTCAGCCNCTGTTGAAACCAATGAACCANANAAANAATTAGCNCC
AGCTTACGCTTTATTAGGTCCAATTCAANANAAATTTGTCTATATTTCTGATTTCATTGA
ACCAATTGCTGATGGNACTGCCGACAATGTTTTCATCTCAAAATCATATGATGCTACCTC
TCNCTTTGAAACCNCTTGTGAAGATGTCATGGATATGNACAANAGAATCNCTGGTAAAGA
TTTAGTTCTCNCTGTTCATCCAAATATTCTTAAACAACAANAANAAGCTGGNAACCAAGA
ACAAGAAGAACAATAAAAAAAAAAATAAAAAAATAAAAATAAAAAAATAAAAATAAAAAA
AATAAATAAATAATAATTAAATTATAATAAAATAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
KFFFLYXIFNTPNIYLYLYIYIFFFILFSHPILYKLIFFFFXXQFKKK---

---XKKIIKVTXEGETVTCDKIVCDPSYFPNKVQXKGKIIRSICILNGPIPSXDXKFESL
QIIIPQKQVKRQSXMXVGVMSSAHCVCPKGKYIAIVSAXVETNEPXKXLAPAYALLGPIQ
XKFVYISDFIEPIADGTADNVFISKSYDATSXFETXCEDVMDMXXRIXGKDLVLXVHPNI
LKQQXXAGNQEQEEQ*kkk*knknkkikikkink**lnynki
Translated Amino Acid sequence (All Frames) 
Frame A:
kifffixyf*htkyififiylyiffyfiftpysi*infffflxpi*kk---

---xkeny*syx*r*ncnm**ncl*siifpk*sskxr*nysfnlyfewsnsix**xi*it
pnhypxkts*tsixyxrwcyvirslcxskg*ihchrfsxc*nq*txxxisxslrfirsns
xxiclyf*fh*tnc*wxcrqcfhlkii*cylsl*nxl*rchgyxqxnxw*rfssxcssky
s*ttxxswxprtrrtikkkikk*k*knknkknk*iiikl**n

Frame B:
KFFFLYXIFNTPNIYLYLYIYIFFFILFSHPILYKLIFFFFXXQFKKK---

---XKKIIKVTXEGETVTCDKIVCDPSYFPNKVQXKGKIIRSICILNGPIPSXDXKFESL
QIIIPQKQVKRQSXMXVGVMSSAHCVCPKGKYIAIVSAXVETNEPXKXLAPAYALLGPIQ
XKFVYISDFIEPIADGTADNVFISKSYDATSXFETXCEDVMDMXXRIXGKDLVLXVHPNI
LKQQXXAGNQEQEEQ*kkk*knknkkikikkink**lnynki

Frame C:
nfffyxvflthqiyiyiyifiyfflfyfhtlfyin*fffffxxnlkkk---

---krkllklhxkvkl*hviklfvihhisqikfkxkvklfvqfvf*mvqfhlxixnlnhs
kslsxknklnvnxixtlvlchplivxvqrvntlpsfqpllkpmnxxxn*xqltly*vqfx
xnlsiflislnqllmxlptmfssqnhmmlplxlkplvkmswixtxeslvki*fslfiqif
lnnxxklxtknkknnkkknkkikikk*k*kk*innn*iiik*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFN656 (AFN656Q) /CSM/AF/AFN6-C/AFN656Q.Seq.d/                        993   0.0
SFI510 (SFI510Q) /CSM/SF/SFI5-A/SFI510Q.Seq.d/                        973   0.0
SSD420 (SSD420Q) /CSM/SS/SSD4-A/SSD420Q.Seq.d/                        971   0.0
SFJ116 (SFJ116Q) /CSM/SF/SFJ1-A/SFJ116Q.Seq.d/                        969   0.0
AFM524 (AFM524Q) /CSM/AF/AFM5-A/AFM524Q.Seq.d/                        969   0.0
SFE141 (SFE141Q) /CSM/SF/SFE1-B/SFE141Q.Seq.d/                        965   0.0
SFD219 (SFD219Q) /CSM/SF/SFD2-A/SFD219Q.Seq.d/                        963   0.0
AFM447 (AFM447Q) /CSM/AF/AFM4-B/AFM447Q.Seq.d/                        963   0.0
AFN874 (AFN874Q) /CSM/AF/AFN8-D/AFN874Q.Seq.d/                        959   0.0
SFI237 (SFI237Q) /CSM/SF/SFI2-B/SFI237Q.Seq.d/                        957   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BI315670|BI315670.1 saf75f12.y1 Gm-c1078 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1078-2184 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    56  7e-04  1
AI437629|AI437629.1 sa37e09.y1 Gm-c1004 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1004-1505 5' similar to TR:O24653 O24653 GDI2. ;, mRNA sequence.    56  7e-04  1
BU964562|BU964562.1 sas99a12.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-12311 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    56  7e-04  1
AW397479|AW397479.1 sg79d05.y1 Gm-c1007 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1007-2626 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    56  7e-04  1
BE800797|BE800797.1 sq98c12.y1 Gm-c1049 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1049-1199 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    56  7e-04  1
BI972870|BI972870.1 sai84a09.y1 Gm-c1065 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1065-7338 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    44  0.17  2
AW306227|AW306227.1 se47g11.y1 Gm-c1017 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1017-2109 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    48  0.18  1
AJ005836|AJ005836.1 Cicer arietinum mRNA for GDP dissociation inhibitor (gdi).    38  0.53  2
BX469931|BX469931.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-108D22.    44  2.7  1
BG305330|BG305330.1 fm11h05.x1 Zebrafish adult retina cDNA Danio rerio cDNA clone 4146272 3' similar to SW:GDIB_RAT P50399 RAB GDP DISSOCIATION INHIBITOR BETA ;, mRNA sequence.    44  2.7  1
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF676817_1(EF676817|pid:none) Picea sitchensis clone WS02753_I09...   150   6e-35
EF085049_1(EF085049|pid:none) Picea sitchensis clone WS0297_P09 ...   150   6e-35
AJ401079_1(AJ401079|pid:none) Lycopersicon esculentum partial mR...   146   8e-34
AM710298_1(AM710298|pid:none) Lycopersicon esculentum GDI1 gene ...   146   8e-34
AF251042_1(AF251042|pid:none) Mus musculus rab GDP dissociation ...   145   1e-33
BC095329_1(BC095329|pid:none) Danio rerio GDP dissociation inhib...   145   1e-33
AF130987_1(AF130987|pid:none) Rattus norvegicus RAB GDP dissocia...   144   3e-33
(O97555) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   144   3e-33
(P50398) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   144   3e-33
X74402_1(X74402|pid:none) R.norvegicus rab GDI alpha mRNA.            144   3e-33
protein update 2009. 4.25
PSORT 

 psg: 1.01  gvh: 0.51  alm: 0.30  top: 0.60  tms: 0.07  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.02  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AFN656 is nuc
5' end seq. ID AFN656F
5' end seq. 
>AFN656F.Seq
AAAATTTTTTTTTTTATATNGTATTTTTAACACACCAAATATATATTTATATTTATATAT
TTATATATTTTTTTTTATTTTATTTTCACACCCTATTCTATATAAATTAATTTTTTTTTT
TTTTTANCNCCAATTTAAAAAAAAAA----------
Length of 5' end seq. 146
3' end seq. ID AFN656Z
3' end seq. 
>AFN656Z.Seq
----------ANAAAAGAAAATTATTAAAGTTACATNTGAAGGTGAAACTGTAACATGTG
ATAAAATTGTTTGTGATCCATCATATTTCCCAAATAAAGTTCAAAANAAAGGTAAAATTA
TTCGTTCAATTTGTATTTTGAATGGTCCAATTCCATCTGNTGATAANAAATTTGAATCAC
TCCAAATCATTATCCCNCAAAAACAAGTTAAACGTCAATCANATATGNACGTTGGTGTTA
TGTCATCCGCTCATTGTGTNTGTCCAAAGGGTAAATACATTGCCATCGTTTCAGCCNCTG
TTGAAACCAATGAACCANANAAANAATTAGCNCCAGCTTACGCTTTATTAGGTCCAATTC
AANANAAATTTGTCTATATTTCTGATTTCATTGAACCAATTGCTGATGGNACTGCCGACA
ATGTTTTCATCTCAAAATCATATGATGCTACCTCTCNCTTTGAAACCNCTTGTGAAGATG
TCATGGATATGNACAANAGAATCNCTGGTAAAGATTTAGTTCTCNCTGTTCATCCAAATA
TTCTTAAACAACAANAANAAGCTGGNAACCAAGAACAAGAAGAACAATAAAAAAAAAAAT
AAAAAAATAAAAATAAAAAAATAAAAATAAAAAAAATAAATAAATAATAATTAAATTATA
ATAAAATAA
Length of 3' end seq. 659
Connected seq. ID AFN656P
Connected seq. 
>AFN656P.Seq
AAAATTTTTTTTTTTATATNGTATTTTTAACACACCAAATATATATTTATATTTATATAT
TTATATATTTTTTTTTATTTTATTTTCACACCCTATTCTATATAAATTAATTTTTTTTTT
TTTTTANCNCCAATTTAAAAAAAAAA----------ANAAAAGAAAATTATTAAAGTTAC
ATNTGAAGGTGAAACTGTAACATGTGATAAAATTGTTTGTGATCCATCATATTTCCCAAA
TAAAGTTCAAAANAAAGGTAAAATTATTCGTTCAATTTGTATTTTGAATGGTCCAATTCC
ATCTGNTGATAANAAATTTGAATCACTCCAAATCATTATCCCNCAAAAACAAGTTAAACG
TCAATCANATATGNACGTTGGTGTTATGTCATCCGCTCATTGTGTNTGTCCAAAGGGTAA
ATACATTGCCATCGTTTCAGCCNCTGTTGAAACCAATGAACCANANAAANAATTAGCNCC
AGCTTACGCTTTATTAGGTCCAATTCAANANAAATTTGTCTATATTTCTGATTTCATTGA
ACCAATTGCTGATGGNACTGCCGACAATGTTTTCATCTCAAAATCATATGATGCTACCTC
TCNCTTTGAAACCNCTTGTGAAGATGTCATGGATATGNACAANAGAATCNCTGGTAAAGA
TTTAGTTCTCNCTGTTCATCCAAATATTCTTAAACAACAANAANAAGCTGGNAACCAAGA
ACAAGAAGAACAATAAAAAAAAAAATAAAAAAATAAAAATAAAAAAATAAAAATAAAAAA
AATAAATAAATAATAATTAAATTATAATAAAATAA
Length of connected seq. 805
Full length Seq ID -
Full length Seq. -
Length of full length seq. -