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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFN648
Library AF
(Link to library)
Clone ID AFN648
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16513-1
Original site URL
Representative seq. ID AFN648E
(Link to Original site)
Representative DNA sequence 
>AFN648 (AFN648Q) /CSM/AF/AFN6-B/AFN648Q.Seq.d/
AATTAATAATACATCCACAAAGAATGAAAATATTATTATTTGTTACATTAATTGCATTGG
CATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGACAACTTTG
ATACAGTTGTTGATGGTTCAAAAACTGTATTTGTAAAATTCTATGCACCATGGTGTGGCC
ATTGTAAAAAACTTGCACCAGATTTTGAAATCCTTGCTGACACATTCGCACCAGTTTCCA
ACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCTCTGTAGTA
AATATGATGTTTCTGGTTATCCAACCCTCAAAATCTTTGACAAATCAACCACTGCCAAAG
ATTACAACGGTGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCACGCCAAAA
CCAACGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATCCAAATTTT
GATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGTGT
GGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAA
AAAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCC
AAATATGGTGTCACTGGTTTCCCAACACTCAAATGGTTCGGTAAACAAAGCAAAGACGGT
GAAAAATATGAACAAGGTCGTGATCTTGATACTTTCATCAACTACATCAACAAACAAGCT
GGTGTTAACCGTGTCAAAGGTGGTAAACTCGCTGTTGGTGCTGGTCGTGTTGAACAACTC
GATACTATTGCCACTGAATTCATCGCTGCCGCTGCTGAAGTCCGTAAGGAACTCGTCAAG
AAAGCTCAAACCGTCGTTGATTCTCTCCCAGAAGAATTAAGAACTGAAGGTTCATACTAC
GTCAAAGTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAACCACTGAAATCGCT
CGTATCACCAAATTAGTCTCTGGTTCAATGTCTGGAAAGAAAGCTGATGAATTTGCCAAG
AAACTCAATATTTTAGAATCTTTCAAATCAAAATAAATTAAATTTAATTTAGAAAATAAA
AAATAAAATAAAAGAAAAAAAATAAAA
sequence update 2001. 6. 9
Translated Amino Acid sequence 
n**yihke*kyyyllh*lhwhllpyaqqkemllfyhqttliqllmvqklyl*nsmhhgva
ivknlhqilksllthshqfptkl*lpklivtklttklsvvnmmflviqpsksltnqplpk
ittvldqlmny*lisiitpkptlk*RKHHQMLLIYHHPNFDSVVLDKSKNVLVEFYAPWC
GHCKKLMPDYEILGNTYANEKDVVIAKIDCDAADNKAICSKYGVTGFPTLKWFGKQSKDG
EKYEQGRDLDTFINYINKQAGVNRVKGGKLAVGAGRVEQLDTIATEFIAAAAEVRKELVK
KAQTVVDSLPEELRTEGSYYVKVMKTIAEKSIDFVTTEIARITKLVSGSMSGKKADEFAK
KLNILESFKSK*ikfnlenkk*nkrkkik
Translated Amino Acid sequence (All Frames) 
Frame A:
n**yihke*kyyyllh*lhwhllpyaqqkemllfyhqttliqllmvqklyl*nsmhhgva
ivknlhqilksllthshqfptkl*lpklivtklttklsvvnmmflviqpsksltnqplpk
ittvldqlmny*lisiitpkptlk*RKHHQMLLIYHHPNFDSVVLDKSKNVLVEFYAPWC
GHCKKLMPDYEILGNTYANEKDVVIAKIDCDAADNKAICSKYGVTGFPTLKWFGKQSKDG
EKYEQGRDLDTFINYINKQAGVNRVKGGKLAVGAGRVEQLDTIATEFIAAAAEVRKELVK
KAQTVVDSLPEELRTEGSYYVKVMKTIAEKSIDFVTTEIARITKLVSGSMSGKKADEFAK
KLNILESFKSK*ikfnlenkk*nkrkkik


Frame B:
inntstkneniiicyincigicclmlsrrkcccfitrql*ysc*wfkncickilctmvwp
l*ktctrf*npc*hirtsfqqscncqs*l*ps*qqssl**i*cfwlsnpqnl*qinhcqr
lqrc*is**iinlyq*srqnqr*skestikcc*fitiqiliqlf*inqrmf*lnsmhhgv
viaks*cqimkslvtptpmkkmlsspkliamllitkpsapnmvslvsqhsngsvnkaktv
knmnkvvililssttstnklvltvskvvnsllvlvvlnnsillplnsslpllksvrnssr
klkpslilsqkn*elkvhttsks*kpllknqsil*plkslvspn*slvqclerklmnlpr
nsif*nlsnqnklnli*kiknkikekk*


Frame C:
liihpqrmkillfvtlialafvalcsaegnvvvlspdnfdtvvdgsktvfvkfyapwcgh
ckklapdfeiladtfapvsnkvviakvdcdqadnkalcskydvsgyptlkifdksttakd
yngarsvdelltyinnhaktnvkvkkapsnvvdlspskf*fscfr*ikecfs*ilctmvw
slqkvnarl*npw*hlrq*krcchrqn*lrcc**qshllqiwchwfpntqmvr*tkqrr*
ki*trs*s*yfhqlhqqtswc*pcqrw*trcwcwsc*ttryych*ihrcrc*sp*gtrqe
ssnrr*fsprrikn*rfilrqshenhc*kinrfcnh*nrsyhqislwfnvwkes**icqe
tqyfrifqikin*i*frk*kik*kkknk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFN648 (AFN648Q) /CSM/AF/AFN6-B/AFN648Q.Seq.d/                       2187   0.0
VFO241 (VFO241Q) /CSM/VF/VFO2-B/VFO241Q.Seq.d/                       2169   0.0
VFM578 (VFM578Q) /CSM/VF/VFM5-D/VFM578Q.Seq.d/                       2169   0.0
VFM537 (VFM537Q) /CSM/VF/VFM5-B/VFM537Q.Seq.d/                       2169   0.0
VFL370 (VFL370Q) /CSM/VF/VFL3-C/VFL370Q.Seq.d/                       2169   0.0
VFI769 (VFI769Q) /CSM/VF/VFI7-C/VFI769Q.Seq.d/                       2169   0.0
VFI520 (VFI520Q) /CSM/VF/VFI5-A/VFI520Q.Seq.d/                       2169   0.0
VFG408 (VFG408Q) /CSM/VF/VFG4-A/VFG408Q.Seq.d/                       2169   0.0
VFF762 (VFF762Q) /CSM/VF/VFF7-C/VFF762Q.Seq.d/                       2169   0.0
VFF615 (VFF615Q) /CSM/VF/VFF6-A/VFF615Q.Seq.d/                       2169   0.0
own update 2001.12. 2
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.  1251  0.0  8
AF019112|AF019112.1 Dictyostelium discoideum protein disulfide isomerase (dis) mRNA, complete cds.  1243  0.0  3
BX252003|BX252003.1 Pinus pinaster xylem EST, clone PP058D07.     54  2e-19  4
BQ695627|BQ695627.1 NXPV_030_G06_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda cDNA clone NXPV_030_G06 5', mRNA sequence.    58  2e-19  3
AU299541|AU299541.1 Cryptomeria japonica mRNA, clone: CM1065, 5' end, expressed in male cone.    62  3e-13  2
AK113243|AK113243.1 Ciona intestinalis cDNA, clone:ciad019c19, full insert sequence.    54  3e-13  5
CB090389|CB090389.1 gy74e10.g1 Cycad Leaf Library (NYBG) Cycas rumphii cDNA clone gy74e10, mRNA sequence.    80  1e-12  2
CF508720|CF508720.1 USDA-FP_124000-223 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-70_B02 5', mRNA sequence.    68  3e-12  2
CF509886|CF509886.1 USDA-FP_125000-394 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-82_F01 5', mRNA sequence.    68  4e-12  2
BE208912|BE208912.1 GF-FV-P3F1 Marsh grapefruit young flavedo Citrus x paradisi cDNA, mRNA sequence.    68  4e-12  2
dna update 2003. 9.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86IA3) RecName: Full=Protein disulfide-isomerase 1;          S...   397   e-109
AF019112_1(AF019112|pid:none) Dictyostelium discoideum protein d...   395   e-108
EF086125_1(EF086125|pid:none) Picea sitchensis clone WS0285_I21 ...   184   2e-79
DQ222488_1(DQ222488|pid:none) Solanum tuberosum clone 098B03 dis...   176   2e-76
AY730725_1(AY730725|pid:none) Entamoeba histolytica protein disu...   169   1e-75
AB182629_1(AB182629|pid:none) Glycine max Gm pdis-1a mRNA for pr...   167   8e-75
AC087425_4(AC087425|pid:none) Oryza sativa (japonica cultivar-gr...   175   1e-74
T03644(T03644) probable protein disulfide-isomerase (EC 5.3.4.1)...   173   6e-74
EU284948_1(EU284948|pid:none) TSA: Elaeis guineensis EgEfMPOB001...   178   8e-74
AM485099_1(AM485099|pid:none) Vitis vinifera contig VV78X160692....   169   1e-73
protein update 2009. 6.29
PSORT 

 psg: 0.81  gvh: 0.40  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for AFN648 is nuc
5' end seq. ID AFN648F
5' end seq. 
>AFN648F.Seq
AATTAATAATACATCCACAAAGAATGAAAATATTATTATTTGTTACATTAATTGCATTGG
CATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGACAACTTTG
ATACAGTTGTTGATGGTTCAAAAACTGTATTTGTAAAATTCTATGCACCATGGTGTGGCC
ATTGTAAAAAACTTGCACCAGATTTTGAAATCCTTGCTGACACATTCGCACCAGTTTCCA
ACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCTCTGTAGTA
AATATGATGTTTCTGGTTATCCAACCCTCAAAATCTTTGACAAATCAACCACTGCCAAAG
ATTACAACGGTGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCACGCCAAAA
CCAACGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATCAAATTTTG
ATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGTGTG
GTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAAA
AAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCCA
AATATGGTGTCACTGGNTTCCCAACACTCAAA----------
Length of 5' end seq. 692
3' end seq. ID AFN648Z
3' end seq. 
>AFN648Z.Seq
----------GTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATCCAAATTTTG
ATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGTGTG
GTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAAA
AAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCCA
AATATGGTGTCACTGGTTTCCCAACACTCAAATGGTTCGGTAAACAAAGCAAAGACGGTG
AAAAATATGAACAAGGTCGTGATCTTGATACTTTCATCAACTACATCAACAAACAAGCTG
GTGTTAACCGTGTCAAAGGTGGTAAACTCGCTGTTGGTGCTGGTCGTGTTGAACAACTCG
ATACTATTGCCACTGAATTCATCGCTGCCGCTGCTGAAGTCCGTAAGGAACTCGTCAAGA
AAGCTCAAACCGTCGTTGATTCTCTCCCAGAAGAATTAAGAACTGAAGGTTCATACTACG
TCAAAGTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAACCACTGAAATCGCTC
GTATCACCAAATTAGTCTCTGGTTCAATGTCTGGAAAGAAAGCTGATGAATTTGCCAAGA
AACTCAATATTTTAGAATCTTTCAAATCAAAATAAATTAAATTTAATTTAGAAAATAAAA
AATAAAATAAAAGAAAAAAAATAAAA
Length of 3' end seq. 736
Connected seq. ID AFN648P
Connected seq. 
>AFN648P.Seq
AATTAATAATACATCCACAAAGAATGAAAATATTATTATTTGTTACATTAATTGCATTGG
CATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGACAACTTTG
ATACAGTTGTTGATGGTTCAAAAACTGTATTTGTAAAATTCTATGCACCATGGTGTGGCC
ATTGTAAAAAACTTGCACCAGATTTTGAAATCCTTGCTGACACATTCGCACCAGTTTCCA
ACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCTCTGTAGTA
AATATGATGTTTCTGGTTATCCAACCCTCAAAATCTTTGACAAATCAACCACTGCCAAAG
ATTACAACGGTGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCACGCCAAAA
CCAACGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATCAAATTTTG
ATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGTGTG
GTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAAA
AAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCCA
AATATGGTGTCACTGGNTTCCCAACACTCAAA----------GTAAAGAAAGCACCATCA
AATGTTGTTGATTTATCACCATCCAAATTTTGATTCAGTTGTTTTAGATAAATCAAAGAA
TGTTTTAGTTGAATTCTATGCACCATGGTGTGGTCATTGCAAAAAGTTAATGCCAGATTA
TGAAATCCTTGGTAACACCTACGCCAATGAAAAAGATGTTGTCATCGCCAAAATTGATTG
CGATGCTGCTGATAACAAAGCCATCTGCTCCAAATATGGTGTCACTGGTTTCCCAACACT
CAAATGGTTCGGTAAACAAAGCAAAGACGGTGAAAAATATGAACAAGGTCGTGATCTTGA
TACTTTCATCAACTACATCAACAAACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACT
CGCTGTTGGTGCTGGTCGTGTTGAACAACTCGATACTATTGCCACTGAATTCATCGCTGC
CGCTGCTGAAGTCCGTAAGGAACTCGTCAAGAAAGCTCAAACCGTCGTTGATTCTCTCCC
AGAAGAATTAAGAACTGAAGGTTCATACTACGTCAAAGTCATGAAAACCATTGCTGAAAA
ATCAATCGATTTTGTAACCACTGAAATCGCTCGTATCACCAAATTAGTCTCTGGTTCAAT
GTCTGGAAAGAAAGCTGATGAATTTGCCAAGAAACTCAATATTTTAGAATCTTTCAAATC
AAAATAAATTAAATTTAATTTAGAAAATAAAAAATAAAATAAAAGAAAAAAAATAAAA
Length of connected seq. 1428
Full length Seq ID AFN648E
Full length Seq. 
>AFN648E.Seq
AATTAATAATACATCCACAAAGAATGAAAATATTATTATTTGTTACATTAATTGCATTGG
CATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGACAACTTTG
ATACAGTTGTTGATGGTTCAAAAACTGTATTTGTAAAATTCTATGCACCATGGTGTGGCC
ATTGTAAAAAACTTGCACCAGATTTTGAAATCCTTGCTGACACATTCGCACCAGTTTCCA
ACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCTCTGTAGTA
AATATGATGTTTCTGGTTATCCAACCCTCAAAATCTTTGACAAATCAACCACTGCCAAAG
ATTACAACGGTGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCACGCCAAAA
CCAACGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATCCAAATTTT
GATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGTGT
GGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAA
AAAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCC
AAATATGGTGTCACTGGTTTCCCAACACTCAAATGGTTCGGTAAACAAAGCAAAGACGGT
GAAAAATATGAACAAGGTCGTGATCTTGATACTTTCATCAACTACATCAACAAACAAGCT
GGTGTTAACCGTGTCAAAGGTGGTAAACTCGCTGTTGGTGCTGGTCGTGTTGAACAACTC
GATACTATTGCCACTGAATTCATCGCTGCCGCTGCTGAAGTCCGTAAGGAACTCGTCAAG
AAAGCTCAAACCGTCGTTGATTCTCTCCCAGAAGAATTAAGAACTGAAGGTTCATACTAC
GTCAAAGTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAACCACTGAAATCGCT
CGTATCACCAAATTAGTCTCTGGTTCAATGTCTGGAAAGAAAGCTGATGAATTTGCCAAG
AAACTCAATATTTTAGAATCTTTCAAATCAAAATAAATTAAATTTAATTTAGAAAATAAA
AAATAAAATAAAAGAAAAAAAATAAAA
Length of full length seq. 1167