>VHD492 (VHD492Q) /CSM/VH/VHD4-D/VHD492Q.Seq.d/
XXXXXXXXXXCTTCATCAGATCCACAAGAACAATTAAACAATATCCGTGCTATCATCACC
GCTCATGCTGAAAAGAAACCAGAACTCGATGAATTATACACCATTGCTTCTCAACTCGAA
GAAGCTCAAGTTGTCGATAACAAACATACTCAACACAGTTTAGAATCAATTAAATTAAAA
TGGGATAAACTCAATACACTCGCTAAAAAGAATGAACAAGTTGTTGAAGGTGAAATTCTT
GCTAAACAATTAACTGGTGTTACTGCTGAAGAATTAAGTGAATTTAAAGCCTGCTTCTCA
CATTTCGATAAGGACAACGATAACAAATTAAATCGTCTTGAATTCTCCTCTTGCTTGAAG
AGTATCGGAGATGAATTAACTGAAGAACAATTAAATCAAGTCATCAGTAAAATCGATACC
GATGGTAATGGTACCATTTCATTCGAAGAATTTATTGATTACATGGTTTCATCACGTAAA
GGTACAGACAGCGTTGAATCAACTAAAGCTGCATTCAAAGTTATGGCTGAGGATAAAGAT
TTCATTACTGAAGCTCAAATTCGTGCTGCTATCTCNGATTCTAAACAAATTGATTATTTA
CTCGCCAGTATGCCAGCTGTTGAAGGTGGTTTTGACTACAATTCATTTGCNAAAAATTAT
ACCAATAAACAAANA

---SSDPQEQLNNIRAIITAHAEKKPELDELYTIASQLEEAQVVDNKHTQHSLESIKLKW
DKLNTLAKKNEQVVEGEILAKQLTGVTAEELSEFKACFSHFDKDNDNKLNRLEFSSCLKS
IGDELTEEQLNQVISKIDTDGNGTISFEEFIDYMVSSRKGTDSVESTKAAFKVMAEDKDF
ITEAQIRAAISDSKQIDYLLASMPAVEGGFDYNSFAKNYTNKQX

Frame A:
---firstrtikqypcyhhrsc*ketrtr*iihhcfstrrssscr*qtystqfrin*ikm
g*tqytr*ke*tsc*r*nsc*tinwcyc*rik*i*sllltfr*gqr*qikss*illllee
yrr*in*rtiksshq*nryrw*wyhfirriy*lhgfit*ryrqr*in*sciqsyg*g*rf
hy*ssnsccylxf*tn*lftrqyasc*rwf*lqficxklyq*tx


Frame B:
---SSDPQEQLNNIRAIITAHAEKKPELDELYTIASQLEEAQVVDNKHTQHSLESIKLKW
DKLNTLAKKNEQVVEGEILAKQLTGVTAEELSEFKACFSHFDKDNDNKLNRLEFSSCLKS
IGDELTEEQLNQVISKIDTDGNGTISFEEFIDYMVSSRKGTDSVESTKAAFKVMAEDKDF
ITEAQIRAAISDSKQIDYLLASMPAVEGGFDYNSFAKNYTNKQX


Frame C:
---hqihknn*tisvlssplmlkrnqnsmnytplllnskklklsitnilntv*nqln*ng
insihslkrmnkllkvkfllnn*lvlllkn*vnlkpashisirttitn*ivlnspla*rv
semn*lknn*ikssvksipmvmvpfhsknllitwfhhvkvqtalnqlklhsklwlrikis
llklkfvllsxilnkliiyspvcqllkvvlttihlxkiipink

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD492 (VHD492Q) /CSM/VH/VHD4-D/VHD492Q.Seq.d/                       1302   0.0
VHH675 (VHH675Q) /CSM/VH/VHH6-D/VHH675Q.Seq.d/                       1289   0.0
VHH621 (VHH621Q) /CSM/VH/VHH6-A/VHH621Q.Seq.d/                       1287   0.0
SLB191 (SLB191Q) /CSM/SL/SLB1-D/SLB191Q.Seq.d/                       1273   0.0
VHD544 (VHD544Q) /CSM/VH/VHD5-B/VHD544Q.Seq.d/                       1271   0.0
VHP489 (VHP489Q) /CSM/VH/VHP4-D/VHP489Q.Seq.d/                       1241   0.0
VHQ459 (VHQ459Q) /CSM/VH/VHQ4-C/VHQ459Q.Seq.d/                       1235   0.0
VHJ587 (VHJ587Q) /CSM/VH/VHJ5-D/VHJ587Q.Seq.d/                       1231   0.0
VHP480 (VHP480Q) /CSM/VH/VHP4-D/VHP480Q.Seq.d/                       1225   0.0
VHP614 (VHP614Q) /CSM/VH/VHP6-A/VHP614Q.Seq.d/                       1211   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Y00689|Y00689.1 Dictyostelium discoideum mRNA for non-muscular alpha-actinin 3.  1239   0.0    2
EJ432919|EJ432919.1 1093015265976 Global-Ocean-Sampling_GS-28-01-01-1P6-2P0KB marine metagenome genomic clone 1061002350995 3', genomic survey sequence.    48   0.33   1
ER570049|ER570049.1 1093015788544 Global-Ocean-Sampling_GS-36-01-01-2P2KB marine metagenome genomic clone 1061002578638 3', genomic survey sequence.    48   0.33   1
Y18166|Y18166.1 Suberites domuncula mRNA for calmodulin.           48   0.33   1
EK060564|EK060564.1 1092960075826 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061001934601 3', genomic survey sequence.    32   0.90   2
CR940353|CR940353.1 Theileria annulata genomic DNA chromosome 4.    46   1.3    1
AP008983|AP008983.1 Clostridium phage c-st genomic DNA, complete genome.    46   1.3    1
CP000606|CP000606.1 Shewanella loihica PV-4, complete genome.      46   1.3    1
CP000569|CP000569.1 Actinobacillus pleuropneumoniae L20 serotype 5b complete genome.    46   1.3    1
AC155307|AC155307.2 Mus musculus BAC clone RP24-147F17 from 15, complete sequence.    46   1.3    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FADOAA(S00103;A29006) alpha-actinin - slime mold (Dictyostelium ...   414   e-114
BC015620_1(BC015620|pid:none) Homo sapiens actinin, alpha 4, mRN...   143   4e-33
D89980_1(D89980|pid:none) Homo sapiens mRNA for alpha actinin 4,...   143   4e-33
(Q5RCS6) RecName: Full=Alpha-actinin-4; AltName: Full=Non-muscle...   143   4e-33
(O43707) RecName: Full=Alpha-actinin-4; AltName: Full=Non-muscle...   143   4e-33
BC070594_1(BC070594|pid:none) Xenopus laevis hypothetical protei...   143   4e-33
A42162(A42162;S02033;A29555;A28450) alpha-actinin 1 - chicken  &...   142   9e-33
(P05094) RecName: Full=Alpha-actinin-1; AltName: Full=Alpha-acti...   142   9e-33
AK150044_1(AK150044|pid:none) Mus musculus bone marrow macrophag...   140   3e-32
(Q9QXQ0) RecName: Full=Alpha-actinin-4; AltName: Full=Non-muscle...   140   5e-32

 psg: 0.75  gvh: 0.35  alm: 0.53  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 16.0 %: cytoskeletal
	  8.0 %: mitochondrial

>> prediction for VHD492 is nuc

>VHD492Z.Seq
NNNNNNNNNNCTTCATCAGATCCACAAGAACAATTAAACAATATCCGTGCTATCATCACC
GCTCATGCTGAAAAGAAACCAGAACTCGATGAATTATACACCATTGCTTCTCAACTCGAA
GAAGCTCAAGTTGTCGATAACAAACATACTCAACACAGTTTAGAATCAATTAAATTAAAA
TGGGATAAACTCAATACACTCGCTAAAAAGAATGAACAAGTTGTTGAAGGTGAAATTCTT
GCTAAACAATTAACTGGTGTTACTGCTGAAGAATTAAGTGAATTTAAAGCCTGCTTCTCA
CATTTCGATAAGGACAACGATAACAAATTAAATCGTCTTGAATTCTCCTCTTGCTTGAAG
AGTATCGGAGATGAATTAACTGAAGAACAATTAAATCAAGTCATCAGTAAAATCGATACC
GATGGTAATGGTACCATTTCATTCGAAGAATTTATTGATTACATGGTTTCATCACGTAAA
GGTACAGACAGCGTTGAATCAACTAAAGCTGCATTCAAAGTTATGGCTGAGGATAAAGAT
TTCATTACTGAAGCTCAAATTCGTGCTGCTATCTCNGATTCTAAACAAATTGATTATTTA
CTCGCCAGTATGCCAGCTGTTGAAGGTGGTTTTGACTACAATTCATTTGCNAAAAATTAT
ACCAATAAACAAANA