>VHD473 (VHD473Q) /CSM/VH/VHD4-D/VHD473Q.Seq.d/
XXXXXXXXXXCTATACAATGTTATAATTTAACATTGAATGGCGGTGTTTTGTCAGTGGTT
GGCGGGGCACAATCGACATTGGACCTTTCTGATAGTTTATTGTTGAATGGAAACGCAACA
TTATTAATCTCAAATATTAATTCTGATCTACTTTTAAATAAATTAGTGGTTAAAGGTGAT
CTAAAGATTTATTCAAAACAAAGTTTAATTAGATTCGATAGATTGGGTAGAATTTCACCA
CATCTTTTAATTGATGGTGATTGTTTATTCAATGGTGATTTAGAGATTTATTTCAATCCA
AATATTGAATATCATAGTGAATATCATATACTCAAATGTGAAAGTGGTCGTTATTCAAAT
CAATTCATTTCAACAAAGATTTTCTTAAATAATAGTTTAATTAGTTCATCATCTTATCAA
ATTGAACAAACCGATGCAATCTATTTAATCACTGAAGAAATTGAAGAAGATGAAGTTGAA
AAAACTTTAAATGGTTGGACATTATTCACTGTAATTTGTATTACAGCTTTTGCTGTTTTA
ATAATATCTTTTTATCTTTTCTATAAAAAGAAATTTAGAAAACAAAATAATCATTATTTA
CCAGTTGAATTAACTGAAATTGATAATAATAATAATAATTAAAATTAAAAATTAAAAATA
AAAATAAAAAAAAGNAAATAAATTATATATATTTTTTAAAAA

---IQCYNLTLNGGVLSVVGGAQSTLDLSDSLLLNGNATLLISNINSDLLLNKLVVKGDL
KIYSKQSLIRFDRLGRISPHLLIDGDCLFNGDLEIYFNPNIEYHSEYHILKCESGRYSNQ
FISTKIFLNNSLISSSSYQIEQTDAIYLITEEIEEDEVEKTLNGWTLFTVICITAFAVLI
ISFYLFYKKKFRKQNNHYLPVELTEIDNNNNN*n*klkikikkxk*iiyif*k

Frame A:
---ytml*fniewrcfvsgwrgtidigpf**fivewkrniinlky*f*stfk*isg*r*s
kdlfktkfn*ir*ig*nfttsfn*w*lfiqw*frdlfqsky*is**isytqm*kwslfks
ihfnkdflk**fn*fiilsn*tnrcnlfnh*rn*rr*s*knfkwldiihcnlyysfccfn
niflsfl*kei*ktk*slfts*in*n******lklkiknknkkkxinyiyflk


Frame B:
---IQCYNLTLNGGVLSVVGGAQSTLDLSDSLLLNGNATLLISNINSDLLLNKLVVKGDL
KIYSKQSLIRFDRLGRISPHLLIDGDCLFNGDLEIYFNPNIEYHSEYHILKCESGRYSNQ
FISTKIFLNNSLISSSSYQIEQTDAIYLITEEIEEDEVEKTLNGWTLFTVICITAFAVLI
ISFYLFYKKKFRKQNNHYLPVELTEIDNNNNN*n*klkikikkxk*iiyif*k


Frame C:
---ynvii*h*mavfcqwlaghnrhwtflivyc*metqhy*sqililiyf*in*wlkvi*
rfiqnkv*ldsidwvefhhif*lmvivysmvi*rfisiqilniivniiysnvkvvviqin
sfqqrfs*iiv*lvhhliklnkpmqsi*slkklkkmklkkl*mvghysl*fvlqlllf**
ylfifsikrnlenkiiiiyqln*lkliiiiiikikn*k*k*kkxnklyiffk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD473 (VHD473Q) /CSM/VH/VHD4-D/VHD473Q.Seq.d/                       1201   0.0
VFI257 (VFI257Q) /CSM/VF/VFI2-C/VFI257Q.Seq.d/                       1193   0.0
VHK272 (VHK272Q) /CSM/VH/VHK2-C/VHK272Q.Seq.d/                       1183   0.0
VHG857 (VHG857Q) /CSM/VH/VHG8-C/VHG857Q.Seq.d/                       1179   0.0
CHM526 (CHM526Q) /CSM/CH/CHM5-B/CHM526Q.Seq.d/                       1179   0.0
VFJ624 (VFJ624Q) /CSM/VF/VFJ6-A/VFJ624Q.Seq.d/                       1023   0.0
VHE689 (VHE689Q) /CSM/VH/VHE6-D/VHE689Q.Seq.d/                        589   e-167
VSE576 (VSE576Q) /CSM/VS/VSE5-D/VSE576Q.Seq.d/                         40   0.044
VHJ573 (VHJ573Q) /CSM/VH/VHJ5-D/VHJ573Q.Seq.d/                         40   0.044
SHI793 (SHI793Q) /CSM/SH/SHI7-D/SHI793Q.Seq.d/                         40   0.044

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

DV124476|DV124476.1 CV03039A2G06.f1 CV03-normalized library Euphorbia esula cDNA cloneCV03039A2G06.f1 5, mRNA sequence.    54   0.005  1
AE000784|AE000784.1 Borrelia burgdorferi plasmid lp28-3, complete plasmid sequence.    48   0.023  3
AM285319|AM285319.1 Spiroplasma citri GII3-3X chromosome, contig Contig18.    38   0.33   5
EC346097|EC346097.1 LIVERF089543E18 POSSUM_01-POSSUM-LIVER-2KB Trichosurus vulpecula cDNA clone 1061024741441, mRNA sequence.    48   0.34   1
AC140745|AC140745.1 Rattus norvegicus clone CH230-382H7, WORKING DRAFT SEQUENCE, 42 unordered pieces.    48   0.34   1
AC127979|AC127979.3 Rattus norvegicus clone CH230-356N15, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    40   0.58   2
AC159885|AC159885.2 Mus musculus BAC clone RP24-432B19 from chromosome 12, complete sequence.    34   0.64   5
AC175924|AC175924.1 Strongylocentrotus purpuratus clone R3-12L23, WORKING DRAFT SEQUENCE, 20 unordered pieces.    36   0.72   2
AC181955|AC181955.1 Strongylocentrotus purpuratus clone R3-1004H8, WORKING DRAFT SEQUENCE, 13 unordered pieces.    36   0.72   2
AC180729|AC180729.1 Strongylocentrotus purpuratus clone R3-3083K1, WORKING DRAFT SEQUENCE, 17 unordered pieces.    44   0.73   6

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 775,044,863

 psg: 0.91  gvh: 0.63  alm: 0.18  top: 1.23  tms: 0.07  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.33  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHD473 is nuc

>VHD473Z.Seq
NNNNNNNNNNCTATACAATGTTATAATTTAACATTGAATGGCGGTGTTTTGTCAGTGGTT
GGCGGGGCACAATCGACATTGGACCTTTCTGATAGTTTATTGTTGAATGGAAACGCAACA
TTATTAATCTCAAATATTAATTCTGATCTACTTTTAAATAAATTAGTGGTTAAAGGTGAT
CTAAAGATTTATTCAAAACAAAGTTTAATTAGATTCGATAGATTGGGTAGAATTTCACCA
CATCTTTTAATTGATGGTGATTGTTTATTCAATGGTGATTTAGAGATTTATTTCAATCCA
AATATTGAATATCATAGTGAATATCATATACTCAAATGTGAAAGTGGTCGTTATTCAAAT
CAATTCATTTCAACAAAGATTTTCTTAAATAATAGTTTAATTAGTTCATCATCTTATCAA
ATTGAACAAACCGATGCAATCTATTTAATCACTGAAGAAATTGAAGAAGATGAAGTTGAA
AAAACTTTAAATGGTTGGACATTATTCACTGTAATTTGTATTACAGCTTTTGCTGTTTTA
ATAATATCTTTTTATCTTTTCTATAAAAAGAAATTTAGAAAACAAAATAATCATTATTTA
CCAGTTGAATTAACTGAAATTGATAATAATAATAATAATTAAAATTAAAAATTAAAAATA
AAAATAAAAAAAAGNAAATAAATTATATATATTTTTTAAAAA