>VHD430 (VHD430Q) /CSM/VH/VHD4-B/VHD430Q.Seq.d/
GATACCAATTTCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATG
AAAACTATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCA
ACATATGGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAA
AAACCACGTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCA
AAGAAACAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGAT
AATTTAATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATT
ACAACATTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCT
AAAACTTATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGAT
ATTCATCATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTT
ATCGAAGGTAGTCCAAATAAGATTCCAATGATTTATGGTTTAGATTCAGTACATGGTGCA
AATTATGTTCACAAAGCAACTTTATTCCCACATAATACTGGXXXXXXXXXXCAAGAATTT
TACCACCAGATTTCGTNTACAGTTGTGCTGCAGTTTTAATGGCTTATNTCCCAGGTAGTG
AAGGTGGTAAACCAATCGCCAACATTTTCATGGGTAATGTAAATCCATCTGGTCGTNTTC
CTTTAACTTATCCAGGTACTACTGGTGATATCGGTGTTCCATATTACCACAAATACAGTG
AAAATGGTGTCACCACTCCACTNTTCCAATTTGGTGATGGTNTTTNTTATACCACTTTTA
ACTATACCAATNTCGCCTGTAGCAATTGTAAACCAATCTNTGGTCAATNTGGTAACTACA
CTGGTGTTTTAGGTCAATCCTATACTTTCACTGTCACTGTCACAAACAATGGTAACGTTC
AAGGTAAAGACTCTGTTCTCTTATATCTCTCTGATCTTTGGGCTCAAGTTACTCCANAAG
TTAAAATGTTAANAGGTTTCCAAAAAGTTGATNTTATGCCAGCTAAATCTCAACAAATCT
CTTTTACCCTCAATGCCTATGAATTCTCCTTTATTGGTGTCGATAATAAAATTACTTTAG
AATCTGGTCAATTCATCATTANGGTTGGTAATCAACAATTAGGTTTATATTTACAATAAA
AAAAAAAATTAAAAAAATTAAATTAAATTAAATAGTTAAAAAT

DTNFNEYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTYGSSIRVSIVGGEEAEVIE
KPRTFGNKRELKLEYSQIYPKKQLNQENINFMSARDTFVDNLMSKMSITEKIGQMTQLDI
TTLTSPNTITINETTLAYYAKTYYIGSYLNSPVSGGLAGDIHHINSSVWLDMINTIQTIV
IEGSPNKIPMIYGLDSVHGANYVHKATLFPHNT---

---RILPPDFVYSCAAVLMAYXPGSEGGKPIANIFMGNVNPSGRXPLTYPGTTGDIGVPY
YHKYSENGVTTPLFQFGDGXXYTTFNYTNXACSNCKPIXGQXGNYTGVLGQSYTFTVTVT
NNGNVQGKDSVLLYLSDLWAQVTPXVKMLXGFQKVDXMPAKSQQISFTLNAYEFSFIGVD
NKITLESGQFIIXVGNQQLGLYLQ*kkklkkln*ik*lk

Frame A:
DTNFNEYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTYGSSIRVSIVGGEEAEVIE
KPRTFGNKRELKLEYSQIYPKKQLNQENINFMSARDTFVDNLMSKMSITEKIGQMTQLDI
TTLTSPNTITINETTLAYYAKTYYIGSYLNSPVSGGLAGDIHHINSSVWLDMINTIQTIV
IEGSPNKIPMIYGLDSVHGANYVHKATLFPHNT---

---qefyhqisxtvvlqf*wlxsqvvkvvnqsptfswvm*ihlvvfl*liqvllvisvfh
ittntvkmvsplhxsnlvmvfxiplltipxspvaivnqsxvnxvttlvf*vnpilslsls
qtmvtfkvktlfsyislifglkllxklkc*xvskklxlcqlnlnksllpsmpmnspllvs
iikll*nlvnsslxlvinn*vyiynkkkn*kn*iklns*k

Frame B:
ipismni*lnsilyiyikr*kllkvyfychc***iy**vqhmevqle*allevkklkslk
nhvlleikensn*sivkfiqrnn*ikkilisclpeilllii*cqkcqslkklvk*lnwil
qh*hhqiqlqlmkqh*himlkliildhi*ihqyqvv*qvifiilivvfg*i*lilfkpll
skvvqirfq*fmv*iqymvqimftkqlyshiil---

---knfttrfrxqlccsfnglxpr**rw*tnrqhfhg*cksiwsxsfnlsryyw*yrcsi
lpqiq*kwchhstxpiw*wxxlyhf*lyqxrl*ql*tnlwsxw*lhwcfrsilyfhchch
kqw*rsr*rlcslisl*slgssysxs*nvxrfpks*xyas*istnlfypqcl*illywcr
**nyfriwsihhxgw*stirfiftikkkikkikln*ivkn

Frame C:
yqfq*ifn*iqfyiyi*kdeny*kfiftvivdsefinkfniwkfn*skhcwr*rs*sh*k
ttyfwk*krtqirv*snlsketiksrky*fhvcqryfc**fnvknvnh*knwsndsigyy
ninitkynyn**nnisilc*nllywiifkftsirwfsr*yssy***clvryd*yysnhcy
rr*sk*dsndlwfrfstwcklcsqsnfipt*yw---

---RILPPDFVYSCAAVLMAYXPGSEGGKPIANIFMGNVNPSGRXPLTYPGTTGDIGVPY
YHKYSENGVTTPLFQFGDGXXYTTFNYTNXACSNCKPIXGQXGNYTGVLGQSYTFTVTVT
NNGNVQGKDSVLLYLSDLWAQVTPXVKMLXGFQKVDXMPAKSQQISFTLNAYEFSFIGVD
NKITLESGQFIIXVGNQQLGLYLQ*kkklkkln*ik*lk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD430 (VHD430Q) /CSM/VH/VHD4-B/VHD430Q.Seq.d/                       2252   0.0
VHQ110 (VHQ110Q) /CSM/VH/VHQ1-A/VHQ110Q.Seq.d/                       1168   0.0
VHO350 (VHO350Q) /CSM/VH/VHO3-C/VHO350Q.Seq.d/                       1168   0.0
VHL744 (VHL744Q) /CSM/VH/VHL7-B/VHL744Q.Seq.d/                       1168   0.0
VHK201 (VHK201Q) /CSM/VH/VHK2-A/VHK201Q.Seq.d/                       1168   0.0
VHI201 (VHI201Q) /CSM/VH/VHI2-A/VHI201Q.Seq.d/                       1168   0.0
VHH381 (VHH381Q) /CSM/VH/VHH3-D/VHH381Q.Seq.d/                       1168   0.0
VHG420 (VHG420Q) /CSM/VH/VHG4-A/VHG420Q.Seq.d/                       1168   0.0
VHG391 (VHG391Q) /CSM/VH/VHG3-D/VHG391Q.Seq.d/                       1168   0.0
VHC215 (VHC215Q) /CSM/VH/VHC2-A/VHC215Q.Seq.d/                       1168   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L21014|L21014.1 Dictyostelium discoideum beta-glucosidase mRNA, complete cds.  1164   0.0    2
BX293980|BX293980.2 Mycoplasma mycoides subsp. mycoides SC str. PG1, complete genome.    32   0.026  16
EH013950|EH013950.1 USDA-FP_186699 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt064_C10 5', mRNA sequence.    40   0.039  2
BG139849|BG139849.1 EST480291 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP14B10 5' sequence, mRNA sequence.    48   0.13   2
CN215175|CN215175.1 28949 Suspension culture Solanum tuberosum cDNA, mRNA sequence.    40   0.30   3
AL403717|AL403717.1 T7 end of clone AT0AA010D11 of library AT0AA from strain CBS 4311 of Saccharomyces servazzii.    38   0.57   3
EB503495|EB503495.1 lmos2p04e08_SP6 Striped sea bream hepatic library Lithognathus mormyrus cDNA, mRNA sequence.    36   0.62   2
BQ969550|BQ969550.1 QHB38N02.yg.ab1 QH_ABCDI sunflower RHA801 Helianthus annuus cDNA clone QHB38N02, mRNA sequence.    48   0.64   1
AC073286|AC073286.5 Homo sapiens chromosome 3 clone RP11-298M2, *** SEQUENCING IN PROGRESS ***, 47 unordered pieces.    48   0.64   1
AC067915|AC067915.2 Homo sapiens clone RP11-131G15, WORKING DRAFT SEQUENCE, 21 unordered pieces.    48   0.64   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A49881(A49881)beta-glucosidase (EC 3.2.1.21) precursor, lysosoma...   350   5e-95
CP000159_2777(CP000159|pid:none) Salinibacter ruber DSM 13855, c...   116   2e-24
CP000083_2301(CP000083|pid:none) Colwellia psychrerythraea 34H, ...   105   4e-21
CP000383_2239(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   101   7e-20
CP000383_3519(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   100   1e-19
CP000685_3497(CP000685|pid:none) Flavobacterium johnsoniae UW101...    98   6e-19
CP000388_3699(CP000388|pid:none) Pseudoalteromonas atlantica T6c...    98   7e-19
CP001146_680(CP001146|pid:none) Dictyoglomus thermophilum H-6-12...    96   2e-18
CP001251_792(CP001251|pid:none) Dictyoglomus turgidum DSM 6724, ...    96   2e-18
CP000383_2243(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...    91   9e-17

 psg: 0.75  gvh: 0.73  alm: 0.26  top: 0.07  tms: 0.07  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.68  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 20.0 %: Golgi
	 20.0 %: endoplasmic reticulum
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane

>> prediction for VHD430 is cyt

>VHD430F.Seq
GATACCAATTTCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATG
AAAACTATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCA
ACATATGGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAA
AAACCACGTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCA
AAGAAACAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGAT
AATTTAATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATT
ACAACATTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCT
AAAACTTATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGAT
ATTCATCATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTT
ATCGAAGGTAGTCCAAATAAGATTCCAATGATTTATGGTTTAGATTCAGTACATGGTGCA
AATTATGTTCACAAAGCAACTTTATTCCCACATAATACTGGNNNNNNNNNN

>VHD430Z.Seq
NNNNNNNNNNCAAGAATTTTACCACCAGATTTCGTNTACAGTTGTGCTGCAGTTTTAATG
GCTTATNTCCCAGGTAGTGAAGGTGGTAAACCAATCGCCAACATTTTCATGGGTAATGTA
AATCCATCTGGTCGTNTTCCTTTAACTTATCCAGGTACTACTGGTGATATCGGTGTTCCA
TATTACCACAAATACAGTGAAAATGGTGTCACCACTCCACTNTTCCAATTTGGTGATGGT
NTTTNTTATACCACTTTTAACTATACCAATNTCGCCTGTAGCAATTGTAAACCAATCTNT
GGTCAATNTGGTAACTACACTGGTGTTTTAGGTCAATCCTATACTTTCACTGTCACTGTC
ACAAACAATGGTAACGTTCAAGGTAAAGACTCTGTTCTCTTATATCTCTCTGATCTTTGG
GCTCAAGTTACTCCANAAGTTAAAATGTTAANAGGTTTCCAAAAAGTTGATNTTATGCCA
GCTAAATCTCAACAAATCTCTTTTACCCTCAATGCCTATGAATTCTCCTTTATTGGTGTC
GATAATAAAATTACTTTAGAATCTGGTCAATTCATCATTANGGTTGGTAATCAACAATTA
GGTTTATATTTACAATAAAAAAAAAAATTAAAAAAATTAAATTAAATTAAATAGTTAAAA
AT

>VHD430P.Seq
GATACCAATTTCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATG
AAAACTATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCA
ACATATGGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAA
AAACCACGTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCA
AAGAAACAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGAT
AATTTAATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATT
ACAACATTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCT
AAAACTTATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGAT
ATTCATCATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTT
ATCGAAGGTAGTCCAAATAAGATTCCAATGATTTATGGTTTAGATTCAGTACATGGTGCA
AATTATGTTCACAAAGCAACTTTATTCCCACATAATACTGG----------CAAGAATTT
TACCACCAGATTTCGTNTACAGTTGTGCTGCAGTTTTAATGGCTTATNTCCCAGGTAGTG
AAGGTGGTAAACCAATCGCCAACATTTTCATGGGTAATGTAAATCCATCTGGTCGTNTTC
CTTTAACTTATCCAGGTACTACTGGTGATATCGGTGTTCCATATTACCACAAATACAGTG
AAAATGGTGTCACCACTCCACTNTTCCAATTTGGTGATGGTNTTTNTTATACCACTTTTA
ACTATACCAATNTCGCCTGTAGCAATTGTAAACCAATCTNTGGTCAATNTGGTAACTACA
CTGGTGTTTTAGGTCAATCCTATACTTTCACTGTCACTGTCACAAACAATGGTAACGTTC
AAGGTAAAGACTCTGTTCTCTTATATCTCTCTGATCTTTGGGCTCAAGTTACTCCANAAG
TTAAAATGTTAANAGGTTTCCAAAAAGTTGATNTTATGCCAGCTAAATCTCAACAAATCT
CTTTTACCCTCAATGCCTATGAATTCTCCTTTATTGGTGTCGATAATAAAATTACTTTAG
AATCTGGTCAATTCATCATTANGGTTGGTAATCAACAATTAGGTTTATATTTACAATAAA
AAAAAAAATTAAAAAAATTAAATTAAATTAAATAGTTAAAAAT