>VHD310 (VHD310Q) /CSM/VH/VHD3-A/VHD310Q.Seq.d/
CCGGATAAATATGGTTTAAATTTGTTATATTCAACTCCATCAATTTATATAGATGCTGTA
AATGATGCCAATCTAGTATGGGAAGTGAAAACTGATGATCTCTTCCCATATGCTGATAAT
GAATTTAGTTATTGGACTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTACGTTCGT
CAAAATAATGCTCTATTACATGTAGTTGAACAAATGTTGGTAACTAGTAGTAATCTTATG
CCAAGTAGTAGATCTGAACAATTGGTTGATGACATTGTTATAATGAGAGAGGTAATGGGT
ATTGCTCAACATCATGATGCCGTATCAGGAACAGAACAACAACATGTTGCAGATGATTAT
GCAGAACGTCTTTCAATTGGTAATTGTGCTTCATTGGAAACTATTAATACAGTTGTTGGC
ACATTACTAACTGCCAATGGTAATTCAAAATCGGCCGCTGCCACTCCAACCATTTCCTTC
TGCCCATTATTAAATCAAAGTATTTGTCCAGCAACTGATCCACTTTCATCAGGTACCTCA
GTTCCAGTGTTAATTTATAATAGTTTAAGTTGGACTCGTAATGAACCAGTTCGTATTCXX
XXXXXXXXTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCNNCAATCTTCAA
ACGATTGGAATTGTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTAC
CAAATGGTTTANAGATTCAAACTNTACAATGGTTAGATAATCAAGATAATNCCATACTTT
TNNGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCGCNAACTATCA
CTTTAGATCTTTCAACTATCTTTTCAACAATT

PDKYGLNLLYSTPSIYIDAVNDANLVWEVKTDDLFPYADNEFSYWTGYFVSRPALKGYVR
QNNALLHVVEQMLVTSSNLMPSSRSEQLVDDIVIMREVMGIAQHHDAVSGTEQQHVADDY
AERLSIGNCASLETINTVVGTLLTANGNSKSAAATPTISFCPLLNQSICPATDPLSSGTS
VPVLIYNSLSWTRNEPVRI---

---SLSHPLLPMFTTTXQSSNDWNCQYQGVYSPLTSASPLPNGLXIQTXQWLDNQDNXIL
XXIENIYQIDGQDSQDPXTITLDLSTIFSTI

Frame A:
PDKYGLNLLYSTPSIYIDAVNDANLVWEVKTDDLFPYADNEFSYWTGYFVSRPALKGYVR
QNNALLHVVEQMLVTSSNLMPSSRSEQLVDDIVIMREVMGIAQHHDAVSGTEQQHVADDY
AERLSIGNCASLETINTVVGTLLTANGNSKSAAATPTISFCPLLNQSICPATDPLSSGTS
VPVLIYNSLSWTRNEPVRI---

---SLSHPLLPMFTTTXQSSNDWNCQYQGVYSPLTSASPLPNGLXIQTXQWLDNQDNXIL
XXIENIYQIDGQDSQDPXTITLDLSTIFSTI

Frame B:
rinmv*icyiqlhqfi*ml*mmpi*ygk*klmisshmlimnlviglvislvvqh*katfv
kimlyym*lnkcw*lvvilcqvvdlnnwlmtll**er*wvllnimmpyqeqnnnmlqmim
qnvfqlvivlhwklliqllahy*lpmviqnrplplqpfpsahy*ikvfvqqlihfhqvpq
fqc*fiiv*vglvmnqfvf---

---lyhihysqclqplxnlqtigivnikvfthh*lqphlyqmvxrfklyng*iikixpyf
xvlkifiklmvkihkirxlsl*ifqlsfqq

Frame C:
g*iwfkfvifnsinlyrcck*cqssmgsen**slpic***i*lldwlfr*sssierlrss
k*csitcs*tnvgn***syak**i*tig**hcynergngycsts*crirnrtttccr*lc
rtsfnw*lcfigny*yscwhitncqw*fkigrchsnhfllpiikskylssn*stfiryls
ssvnl**fklds**tssy---

---fitsitpnvynhsxifkrlelsisrclltidfsltftkwfxdsnxtmvr*sr*xhtf
xy*kylsn*wsrftrsanyhfrsfnylfnn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD310 (VHD310Q) /CSM/VH/VHD3-A/VHD310Q.Seq.d/                       1622   0.0
CHK567 (CHK567Q) /CSM/CH/CHK5-C/CHK567Q.Seq.d/                       1185   0.0
VHB156 (VHB156Q) /CSM/VH/VHB1-C/VHB156Q.Seq.d/                       1142   0.0
CHJ823 (CHJ823Q) /CSM/CH/CHJ8-A/CHJ823Q.Seq.d/                       1142   0.0
AHD445 (AHD445Q) /CSM/AH/AHD4-B/AHD445Q.Seq.d/                       1128   0.0
VFO607 (VFO607Q) /CSM/VF/VFO6-A/VFO607Q.Seq.d/                        940   0.0
VFO555 (VFO555Q) /CSM/VF/VFO5-C/VFO555Q.Seq.d/                        910   0.0
AHC425 (AHC425Q) /CSM/AH/AHC4-B/AHC425Q.Seq.d/                        900   0.0
VHP583 (VHP583Q) /CSM/VH/VHP5-D/VHP583Q.Seq.d/                        797   0.0
AFL472 (AFL472Q) /CSM/AF/AFL4-C/AFL472Q.Seq.d/                        761   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1179   0.0    1
BF255585|BF255585.2 HVSMEf0007E04f Hordeum vulgare seedling root EST library HVcDNA0007 (Etiolated and unstressed) Hordeum vulgare subsp. vulgare cDNA clone HVSMEf0007E04f, mRNA sequence.    66   2e-06  1
EH757587|EH757587.1 CNSL2408.b1_P01.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSL2408, mRNA sequence.    42   8e-06  2
EH754601|EH754601.1 CNSL13768.b1_P10.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSL13768, mRNA sequence.    42   8e-06  2
EH754563|EH754563.1 CNSL13732.b1_H02.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSL13732, mRNA sequence.    42   8e-06  2
CD197126|CD197126.1 MS1-0094P-V348-B09-U.B MS1-0094 Schistosoma mansoni cDNA clone MS1-0094P-V348-B09.B, mRNA sequence.    58   2e-05  2
CD196653|CD196653.1 MS1-0094G-V359-A10-U.B MS1-0094 Schistosoma mansoni cDNA clone MS1-0094G-V359-A10.B, mRNA sequence.    58   2e-05  2
AI975852|AI975852.1 EST270446 Schistosoma mansoni female, Phil LoVerde/Joe Merrick Schistosoma mansoni cDNA clone SMFAX75 5' end, mRNA sequence.    58   2e-05  2
CD196879|CD196879.1 MS1-0094P-V306-H06-U.B MS1-0094 Schistosoma mansoni cDNA clone MS1-0094P-V306-H06.B, mRNA sequence.    58   2e-05  2
CD196728|CD196728.1 MS1-0094G-V369-A04-U.B MS1-0094 Schistosoma mansoni cDNA clone MS1-0094G-V369-A04.B, mRNA sequence.    58   2e-05  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   401   e-110
M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   400   e-110
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...   164   4e-39
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...   161   3e-38
AB026648_12(AB026648|pid:none) Arabidopsis thaliana genomic DNA,...   159   9e-38
AB011474_23(AB011474|pid:none) Arabidopsis thaliana genomic DNA,...   159   1e-37
EF146940_1(EF146940|pid:none) Populus trichocarpa clone WS01225_...   156   7e-37
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   151   2e-35
AM426888_2(AM426888|pid:none) Vitis vinifera contig VV78X263404....   150   7e-35
BC091843_1(BC091843|pid:none) Danio rerio zgc:110815, mRNA (cDNA...   148   2e-34

 psg: 0.86  gvh: 0.38  alm: 0.42  top: 0.27  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system

>> prediction for VHD310 is cyt

>VHD310F.Seq
CCGGATAAATATGGTTTAAATTTGTTATATTCAACTCCATCAATTTATATAGATGCTGTA
AATGATGCCAATCTAGTATGGGAAGTGAAAACTGATGATCTCTTCCCATATGCTGATAAT
GAATTTAGTTATTGGACTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTACGTTCGT
CAAAATAATGCTCTATTACATGTAGTTGAACAAATGTTGGTAACTAGTAGTAATCTTATG
CCAAGTAGTAGATCTGAACAATTGGTTGATGACATTGTTATAATGAGAGAGGTAATGGGT
ATTGCTCAACATCATGATGCCGTATCAGGAACAGAACAACAACATGTTGCAGATGATTAT
GCAGAACGTCTTTCAATTGGTAATTGTGCTTCATTGGAAACTATTAATACAGTTGTTGGC
ACATTACTAACTGCCAATGGTAATTCAAAATCGGCCGCTGCCACTCCAACCATTTCCTTC
TGCCCATTATTAAATCAAAGTATTTGTCCAGCAACTGATCCACTTTCATCAGGTACCTCA
GTTCCAGTGTTAATTTATAATAGTTTAAGTTGGACTCGTAATGAACCAGTTCGTATTCNN
NNNNNNNN

>VHD310Z.Seq
NNNNNNNNNNTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCNNCAATCTTC
AAACGATTGGAATTGTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTT
ACCAAATGGTTTANAGATTCAAACTNTACAATGGTTAGATAATCAAGATAATNCCATACT
TTTNNGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCGCNAACTAT
CACTTTAGATCTTTCAACTATCTTTTCAACAATT

>VHD310P.Seq
CCGGATAAATATGGTTTAAATTTGTTATATTCAACTCCATCAATTTATATAGATGCTGTA
AATGATGCCAATCTAGTATGGGAAGTGAAAACTGATGATCTCTTCCCATATGCTGATAAT
GAATTTAGTTATTGGACTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTACGTTCGT
CAAAATAATGCTCTATTACATGTAGTTGAACAAATGTTGGTAACTAGTAGTAATCTTATG
CCAAGTAGTAGATCTGAACAATTGGTTGATGACATTGTTATAATGAGAGAGGTAATGGGT
ATTGCTCAACATCATGATGCCGTATCAGGAACAGAACAACAACATGTTGCAGATGATTAT
GCAGAACGTCTTTCAATTGGTAATTGTGCTTCATTGGAAACTATTAATACAGTTGTTGGC
ACATTACTAACTGCCAATGGTAATTCAAAATCGGCCGCTGCCACTCCAACCATTTCCTTC
TGCCCATTATTAAATCAAAGTATTTGTCCAGCAACTGATCCACTTTCATCAGGTACCTCA
GTTCCAGTGTTAATTTATAATAGTTTAAGTTGGACTCGTAATGAACCAGTTCGTATTC--
--------TCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCNNCAATCTTCAA
ACGATTGGAATTGTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTAC
CAAATGGTTTANAGATTCAAACTNTACAATGGTTAGATAATCAAGATAATNCCATACTTT
TNNGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCGCNAACTATCA
CTTTAGATCTTTCAACTATCTTTTCAACAATT