>VHD306 (VHD306Q) /CSM/VH/VHD3-A/VHD306Q.Seq.d/
XXXXXXXXXXAGGTTAAGATTCAAACCTCTGAAAATGAACCAGCTCCATCTTCAAACACT
CAACAAGCTGGTGGTGATGCTGAAGATGATCAATGTGATACCATTTTATCACAACTTCCA
CAACCAAGTGAAATGGCTGGTTACAAAATTAACTCTATTCAATTTGAAAAGGATGATGAT
ACCAATCATCATATCGATTTCATTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCA
ATCAGTCCAGCTGATAAACATAAGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTA
GTTACCACTACTGCCGTAGTTGCTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAA
AATAAAGCTTTGGAGAAATATAAGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGT
TTCGTTGAACCAATTGCCGCCCCAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTT
TGGGATCGTTTCGATGTTGATGGTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAA
AAGAAACATGGTTTGGATATTTCAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTC
TTCACTGATAAGAAAACTAAAGAGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAA
ACCCTCTCAAAGAAAC

---VKIQTSENEPAPSSNTQQAGGDAEDDQCDTILSQLPQPSEMAGYKINSIQFEKDDDT
NHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTTAVVAGFVCIELIKVIQN
KALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRFDVDGDITLKEFLDLFEK
KHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSKK

Frame A:
---g*dsnl*k*tssifkhstsww*c*r*sm*yhfittsttk*ngwlqn*lysi*kg**y
qssyrfhyrylkfksyqlcnqss**t*dqryrr*nhssfsyhycrscwfrlyrid*sysk
*sfgei*ehlyefrypilwfr*tncrpkeqns*rldldtlgsfrc*w*yhsqgvfrsl*k
etwfgyfnall*sytficslh**en*rgkikdenlttl*nplke


Frame B:
---VKIQTSENEPAPSSNTQQAGGDAEDDQCDTILSQLPQPSEMAGYKINSIQFEKDDDT
NHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTTAVVAGFVCIELIKVIQN
KALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRFDVDGDITLKEFLDLFEK
KHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSKK


Frame C:
---lrfkplkmnqlhlqtlnklvvmlkminvipfyhnfhnqvkwlvtkltlfnlkrmmip
iiisislplpqi*elptmqsvqlinirpkvspvksfql*lpllp*llvsfvsn*lklfki
klwrnirapl*i*vshslvslnqlppqrtkfvkaglghfgivsmlmvislsrsf*islkr
nmvwifqcslvklhfymlsslirklkrkd*r*kshnsmkpsqrn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHN723 (VHN723Q) /CSM/VH/VHN7-A/VHN723Q.Seq.d/                       1320   0.0
VHD306 (VHD306Q) /CSM/VH/VHD3-A/VHD306Q.Seq.d/                       1320   0.0
VHB744 (VHB744Q) /CSM/VH/VHB7-B/VHB744Q.Seq.d/                       1320   0.0
VHA440 (VHA440Q) /CSM/VH/VHA4-B/VHA440Q.Seq.d/                       1320   0.0
VHA239 (VHA239Q) /CSM/VH/VHA2-B/VHA239Q.Seq.d/                       1320   0.0
SHC572 (SHC572Q) /CSM/SH/SHC5-C/SHC572Q.Seq.d/                       1320   0.0
CHS124 (CHS124Q) /CSM/CH/CHS1-A/CHS124Q.Seq.d/                       1320   0.0
AHH596 (AHH596Q) /CSM/AH/AHH5-D/AHH596Q.Seq.d/                       1320   0.0
AHD109 (AHD109Q) /CSM/AH/AHD1-A/AHD109Q.Seq.d/                       1320   0.0
SHC317 (SHC317Q) /CSM/SH/SHC3-A/SHC317Q.Seq.d/                       1318   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AZ930776|AZ930776.1 474.dhz58e03.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomycesunisporus genomic clone 474.dhz58e03.s1, genomic survey sequence.    68   1e-13  3
CB933531|CB933531.1 ri31b11.y1 Meloidogyne chitwoodi egg pAMP1 v1 Meloidogyne chitwoodi cDNA 5' similar to TR:Q27481 Q27481 C47E12.5 PROTEIN. [1] ;, mRNA sequence.    64   1e-12  2
ED188983|ED188983.1 AUAC-aav62e08.g1 Ascaris suum whole genome shotgun library (PMAJ_4 GSS) Ascaris suum genomic, genomic survey sequence.    60   9e-05  1
AM562633|AM562633.1 Paracentrotus lividus EST, clone MPMGp1173D192Q, 5' end.    38   1e-04  3
DR174755|DR174755.1 HR0003_N01:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   3e-04  1
DR174741|DR174741.1 HR0003_M08:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   3e-04  1
DR174736|DR174736.1 HR0003_M02:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   3e-04  1
AC134210|AC134210.1 Rattus norvegicus clone CH230-197N6, *** SEQUENCING IN PROGRESS ***, 60 unordered pieces.    58   3e-04  1
AC108277|AC108277.6 Rattus norvegicus clone CH230-27E16, *** SEQUENCING IN PROGRESS ***, 8 unordered pieces.    58   3e-04  1
ES225710|ES225710.1 MpSG_ag4_A08 Myzus persicae, tobacco lineage, aphid salivary gland library Myzus persicae cDNA clone MpSG_ag4_A08, mRNA sequence.    34   5e-04  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55C16) RecName: Full=Ubiquitin-like modifier-activating enzyme...   446   e-124
EF468345_1(EF468345|pid:none) Pisum sativum ubiquitin activating...   244   2e-63
AE017341_129(AE017341|pid:none) Cryptococcus neoformans var. neo...   233   5e-60
FN392321_874(FN392321|pid:none) Pichia pastoris GS115 chromosome...   231   2e-59
CR382138_755(CR382138|pid:none) Debaryomyces hansenii strain CBS...   227   2e-58
BX908809_46(BX908809|pid:none) Neurospora crassa DNA linkage gro...   225   8e-58
CP000496_820(CP000496|pid:none) Pichia stipitis CBS 6054 chromos...   224   2e-57
CR382122_592(CR382122|pid:none) Kluyveromyces lactis strain NRRL...   223   3e-57
(P22515) RecName: Full=Ubiquitin-activating enzyme E1 1;  &S3804...   223   4e-57
AE016819_666(AE016819|pid:none) Ashbya gossypii (= Eremothecium ...   222   7e-57

 psg: 0.75  gvh: 0.36  alm: 0.27  top: 0.43  tms: 0.07  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 24.0 %: nuclear
	 20.0 %: endoplasmic reticulum
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHD306 is cyt

>VHD306Z.Seq
NNNNNNNNNNAGGTTAAGATTCAAACCTCTGAAAATGAACCAGCTCCATCTTCAAACACT
CAACAAGCTGGTGGTGATGCTGAAGATGATCAATGTGATACCATTTTATCACAACTTCCA
CAACCAAGTGAAATGGCTGGTTACAAAATTAACTCTATTCAATTTGAAAAGGATGATGAT
ACCAATCATCATATCGATTTCATTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCA
ATCAGTCCAGCTGATAAACATAAGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTA
GTTACCACTACTGCCGTAGTTGCTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAA
AATAAAGCTTTGGAGAAATATAAGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGT
TTCGTTGAACCAATTGCCGCCCCAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTT
TGGGATCGTTTCGATGTTGATGGTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAA
AAGAAACATGGTTTGGATATTTCAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTC
TTCACTGATAAGAAAACTAAAGAGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAA
ACCCTCTCAAAGAAAC