>VHD271 (VHD271Q) /CSM/VH/VHD2-C/VHD271Q.Seq.d/
AGTTTTAATTATTAATTTCATAATAATAATTTAGTAAAGTAATAAAATGAGTTTTATCGA
TCAAATCCCAAAAAGTTTTATTCCAAAGTTAGATGACTTTAGAAATAAATTAAAACCACT
CGAAGGTTCAGTTGATTATTGTCCAAAGGCTTGGTTTTTAGGTCCAAAAGCAGAAAATTC
TGAATTATTAATGACTCTCCTCAAAAGAGCAATTGATGCTCATCTTCAATCAAGAATCAA
TTTCCACAAAGAAGATGCACCAATTTTCGAAGATTCTTACAAAAAAGATCCTGTATACCT
CAATACAGCAAAAGACATGACCAAAAAAGTTGATGAAGTTTTAAATTTCTTAAAACACTC
TCTTCCATATTCATCCTTTAGATATCAAGGACATATGACATCTGATGTTACTATTGCTGG
TGTAATTGGTGCCACTGCTACTATTTATACAATCCAAACAATGTAACAGTTCAAGCAAGT
CCAGTAACCACATTTATTGAAATGGCAGTTAGTAATGATCTTGCCCGTATGGTTGGTTGG
GAATTTCCAGAAAAAATTCCAAAGTTTAATAACAGACATGAAAAGAAACTTTTTGAAGAT
AAAAATATTATTCCATGCTTCCATTTAACTTXXXXXXXXXXCAAGTGTAGGTATCTATGG
TATTCAAGGCTCAAAAGCTGGTGCCAATGCAGTTGCTGCTTATTTGAGCCATTCAATTAT
GCGTACATCAAAATCTGGTTATGGTATGATCTTAAAGAGATGTTTATTAAGTACAAGACT
CTTCTACTTGAGATTATTATGGTTATGTGGTGATAATGATCAATTCAAAGTTGTTGGTGT
ACCAATTGAACCATCTAAGGAAACCCTCGATAATCTCAAATCAAGATTATACGATGCCAA
TGGTCAAATGAAGAGTGTTGATACAATTTGTCAAGATCAACAACTCGTCGTTGATTTAGC
TGAAGTTGGACCAGATCAAAATATCGTTTGTTTTGGTATTAATTTCAAGGATGCCAATGG
TAAGTGGAATACTGATTACAACAAATACTGTGATGTTAATAATCAAATCTATGAAAGATT
TGATTATAAAAAAGAAGTTGGTATGGCTCAATTTGATTTAATTTTCTCAAATACCGCTTT
CAACGATCAGTATGGTCAAGTATTCCAACAAAGTCTTGCCCAAAGATTTGGTCTCACTAC
CAATGTTCCAGCTTTTGGTAGTAATAAATTCCAATTACCAGTTTTAAGATCAACTGTTAT
GGATGTCTTTGCTAACGAAACCACTAAAGGTTCATTCTTTGATGTTACAATGCCATTGTT
CAAATCAGAATGCGCTAAAATTGTTGCTGCATTTGTTAACGCTTCCAAGGCTTCAAAACC
AACTCTTTAAATTTTAAATTTAATCAAAATAATAAAAAATATATTTTTTATTTATTCATT
CATTGTATTTTTTTAAATTTTAAAA

sfny*fhnnnlvk**nefyrsnpkkfyskvr*l*k*ikttrrfs*llskglvfrsksrkf
*iindspqksn*csssiknqfpqrrctnfrrflqkrscipqyskrhdqks**sfkflktl
ssifil*isrtydi*CYYCWCNWCHCYYLYNPNNVTVQASPVTTFIEMAVSNDLARMVGW
EFPEKIPKFNNRHEKKLFEDKNIIPCFHLT---

---SVGIYGIQGSKAGANAVAAYLSHSIMRTSKSGYGMILKRCLLSTRLFYLRLLWLCGD
NDQFKVVGVPIEPSKETLDNLKSRLYDANGQMKSVDTICQDQQLVVDLAEVGPDQNIVCF
GINFKDANGKWNTDYNKYCDVNNQIYERFDYKKEVGMAQFDLIFSNTAFNDQYGQVFQQS
LAQRFGLTTNVPAFGSNKFQLPVLRSTVMDVFANETTKGSFFDVTMPLFKSECAKIVAAF
VNASKASKPTL*ilnlikiikniffiysfivff*ilk

Frame A:
sfny*fhnnnlvk**nefyrsnpkkfyskvr*l*k*ikttrrfs*llskglvfrsksrkf
*iindspqksn*csssiknqfpqrrctnfrrflqkrscipqyskrhdqks**sfkflktl
ssifil*isrtydi*CYYCWCNWCHCYYLYNPNNVTVQASPVTTFIEMAVSNDLARMVGW
EFPEKIPKFNNRHEKKLFEDKNIIPCFHLT---

---qv*vsmvfkaqklvpmqllli*aiqlcvhqnlvmv*s*rdvy*vqdsst*dyygyvv
iminskllvyqlnhlrkpsiisnqdytmpmvk*rvliqfvkinnssli*lkldqikisfv
lvlisrmpmvsgilittntvmliiksmkdliikkklvwlnli*fsqiplstismvkysnk
vlpkdlvslpmfqllvvinsnyqf*dqllwmslltkplkvhslmlqchcsnqnalklllh
lltlprlqnqlfkf*i*sk**kiyflfihslyffkf*

Frame B:
vliinfiiii**snkmsfidqipksfipklddfrnklkplegsvdycpkawflgpkaens
ellmtllkraidahlqsrinfhkedapifedsykkdpvylntakdmtkkvdevlnflkhs
lpyssfryqghmtsdvtiagvigatatiytiqtm*qfkqvq*phllkwqlvmilpvwlvg
nfqkkfqslitdmkrnflkikilfhasi*l---

---kcrylwysrlkswcqcscclfepfnyayikiwlwydlkemfikyktllleiimvmw*
**siqscwctn*ti*gnpr*sqikiircqwsneec*ynlsrsttrr*fs*swtrskyrlf
wy*fqgcqw*vey*lqqil*c**snl*ki*l*krswygsi*fnflkyrfqrsvwssiptk
scpkiwshyqcssfw***ipitsfkincygclc*rnh*rfil*cynaivqirmr*nccci
c*rfqgfktnslnfkfnqnnkkyifylfihciflnfk

Frame C:
f*llis***fskvik*vlsiksqkvlfqs*mtlein*nhskvqliivqrlgf*vqkqkil
ny**lsskeqlmlifnqesistkkmhqfskiltkkilytsiqqkt*pkklmkf*is*ntl
fhihpldikdi*hlmllllv*lvplllfiqskqcnssskssnhiy*ngs***scpygwlg
isrknskv**qt*ketf*r*kyysmlpfn---

---SVGIYGIQGSKAGANAVAAYLSHSIMRTSKSGYGMILKRCLLSTRLFYLRLLWLCGD
NDQFKVVGVPIEPSKETLDNLKSRLYDANGQMKSVDTICQDQQLVVDLAEVGPDQNIVCF
GINFKDANGKWNTDYNKYCDVNNQIYERFDYKKEVGMAQFDLIFSNTAFNDQYGQVFQQS
LAQRFGLTTNVPAFGSNKFQLPVLRSTVMDVFANETTKGSFFDVTMPLFKSECAKIVAAF
VNASKASKPTL*ilnlikiikniffiysfivff*ilk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD271 (VHD271Q) /CSM/VH/VHD2-C/VHD271Q.Seq.d/                       2686   0.0
VHM685 (VHM685Q) /CSM/VH/VHM6-D/VHM685Q.Seq.d/                       1465   0.0
VHB349 (VHB349Q) /CSM/VH/VHB3-C/VHB349Q.Seq.d/                       1443   0.0
VHL430 (VHL430Q) /CSM/VH/VHL4-B/VHL430Q.Seq.d/                       1439   0.0
VHQ102 (VHQ102Q) /CSM/VH/VHQ1-A/VHQ102Q.Seq.d/                       1431   0.0
VHP496 (VHP496Q) /CSM/VH/VHP4-D/VHP496Q.Seq.d/                       1425   0.0
VHH507 (VHH507Q) /CSM/VH/VHH5-A/VHH507Q.Seq.d/                       1419   0.0
VHP458 (VHP458Q) /CSM/VH/VHP4-C/VHP458Q.Seq.d/                       1392   0.0
VHO479 (VHO479Q) /CSM/VH/VHO4-D/VHO479Q.Seq.d/                       1382   0.0
VHL512 (VHL512Q) /CSM/VH/VHL5-A/VHL512Q.Seq.d/                       1378   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AP008207|AP008207.1 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 1.    54   0.012  1
AP003217|AP003217.3 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 1, BAC clone:OSJNBa0094H06.    54   0.012  1
AP003054|AP003054.2 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 1, PAC clone:P0436D06.    54   0.012  1
EJ251361|EJ251361.1 1095344034642 Global-Ocean-Sampling_GS-27-01-01-1P8-2P0KB marine metagenome genomic clone 1061005463530 5', genomic survey sequence.    50   0.18   1
AC093866|AC093866.3 Homo sapiens BAC clone RP11-582C12 from 4, complete sequence.    38   0.40   2
EE663376|EE663376.1 LP-N-N01-1265-LF Lymphoid organ-normalized Penaeus monodon cDNA clone LP-N-N01-1265-LF 5', mRNA sequence.    38   0.53   2
EK092790|EK092790.1 1092962016111 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061002054651 3', genomic survey sequence.    48   0.72   1
DX403757|DX403757.1 GK_Sa0005N15.b1 Gossypioides kirkii WGS library Gossypioides kirkii genomic similar to unknown, genomic survey sequence.    48   0.72   1
AF513725|AF513725.1 Araucaria angustifolia vicilin-like storage protein (Vici) mRNA, complete cds.    48   0.72   1
AY225986|AY225986.1 Muntiacus muntjak mitochondrion, complete genome.    48   0.72   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000113_3785(CP000113|pid:none) Myxococcus xanthus DK 1622, com...   113   2e-23
CP001291_2208(CP001291|pid:none) Cyanothece sp. PCC 7424, comple...   110   1e-22
CP000155_3327(CP000155|pid:none) Hahella chejuensis KCTC 2396, c...    79   4e-18
CP000698_939(CP000698|pid:none) Geobacter uraniireducens Rf4, co...    86   3e-15
CP000559_1042(CP000559|pid:none) Methanocorpusculum labreanum Z,...    64   5e-12
AB086651_1(AB086651|pid:none) Lactobacillus curvatus tdc gene fo...    62   2e-09
AB059363_2(AB059363|pid:none) Tetragenococcus halophilus tnp, td...    61   4e-09
CP000159_814(CP000159|pid:none) Salinibacter ruber DSM 13855, co...    55   2e-08
BX571866_283(BX571866|pid:none) Photorhabdus luminescens subsp. ...    52   3e-08
AB086652_1(AB086652|pid:none) Lactobacillus curvatus tdc gene fo...    57   5e-08

 psg: 0.83  gvh: 0.33  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VHD271 is nuc

>VHD271F.Seq
AGTTTTAATTATTAATTTCATAATAATAATTTAGTAAAGTAATAAAATGAGTTTTATCGA
TCAAATCCCAAAAAGTTTTATTCCAAAGTTAGATGACTTTAGAAATAAATTAAAACCACT
CGAAGGTTCAGTTGATTATTGTCCAAAGGCTTGGTTTTTAGGTCCAAAAGCAGAAAATTC
TGAATTATTAATGACTCTCCTCAAAAGAGCAATTGATGCTCATCTTCAATCAAGAATCAA
TTTCCACAAAGAAGATGCACCAATTTTCGAAGATTCTTACAAAAAAGATCCTGTATACCT
CAATACAGCAAAAGACATGACCAAAAAAGTTGATGAAGTTTTAAATTTCTTAAAACACTC
TCTTCCATATTCATCCTTTAGATATCAAGGACATATGACATCTGATGTTACTATTGCTGG
TGTAATTGGTGCCACTGCTACTATTTATACAATCCAAACAATGTAACAGTTCAAGCAAGT
CCAGTAACCACATTTATTGAAATGGCAGTTAGTAATGATCTTGCCCGTATGGTTGGTTGG
GAATTTCCAGAAAAAATTCCAAAGTTTAATAACAGACATGAAAAGAAACTTTTTGAAGAT
AAAAATATTATTCCATGCTTCCATTTAACTTNNNNNNNNNN

>VHD271Z.Seq
NNNNNNNNNNCAAGTGTAGGTATCTATGGTATTCAAGGCTCAAAAGCTGGTGCCAATGCA
GTTGCTGCTTATTTGAGCCATTCAATTATGCGTACATCAAAATCTGGTTATGGTATGATC
TTAAAGAGATGTTTATTAAGTACAAGACTCTTCTACTTGAGATTATTATGGTTATGTGGT
GATAATGATCAATTCAAAGTTGTTGGTGTACCAATTGAACCATCTAAGGAAACCCTCGAT
AATCTCAAATCAAGATTATACGATGCCAATGGTCAAATGAAGAGTGTTGATACAATTTGT
CAAGATCAACAACTCGTCGTTGATTTAGCTGAAGTTGGACCAGATCAAAATATCGTTTGT
TTTGGTATTAATTTCAAGGATGCCAATGGTAAGTGGAATACTGATTACAACAAATACTGT
GATGTTAATAATCAAATCTATGAAAGATTTGATTATAAAAAAGAAGTTGGTATGGCTCAA
TTTGATTTAATTTTCTCAAATACCGCTTTCAACGATCAGTATGGTCAAGTATTCCAACAA
AGTCTTGCCCAAAGATTTGGTCTCACTACCAATGTTCCAGCTTTTGGTAGTAATAAATTC
CAATTACCAGTTTTAAGATCAACTGTTATGGATGTCTTTGCTAACGAAACCACTAAAGGT
TCATTCTTTGATGTTACAATGCCATTGTTCAAATCAGAATGCGCTAAAATTGTTGCTGCA
TTTGTTAACGCTTCCAAGGCTTCAAAACCAACTCTTTAAATTTTAAATTTAATCAAAATA
ATAAAAAATATATTTTTTATTTATTCATTCATTGTATTTTTTTAAATTTTAAAA

>VHD271P.Seq
AGTTTTAATTATTAATTTCATAATAATAATTTAGTAAAGTAATAAAATGAGTTTTATCGA
TCAAATCCCAAAAAGTTTTATTCCAAAGTTAGATGACTTTAGAAATAAATTAAAACCACT
CGAAGGTTCAGTTGATTATTGTCCAAAGGCTTGGTTTTTAGGTCCAAAAGCAGAAAATTC
TGAATTATTAATGACTCTCCTCAAAAGAGCAATTGATGCTCATCTTCAATCAAGAATCAA
TTTCCACAAAGAAGATGCACCAATTTTCGAAGATTCTTACAAAAAAGATCCTGTATACCT
CAATACAGCAAAAGACATGACCAAAAAAGTTGATGAAGTTTTAAATTTCTTAAAACACTC
TCTTCCATATTCATCCTTTAGATATCAAGGACATATGACATCTGATGTTACTATTGCTGG
TGTAATTGGTGCCACTGCTACTATTTATACAATCCAAACAATGTAACAGTTCAAGCAAGT
CCAGTAACCACATTTATTGAAATGGCAGTTAGTAATGATCTTGCCCGTATGGTTGGTTGG
GAATTTCCAGAAAAAATTCCAAAGTTTAATAACAGACATGAAAAGAAACTTTTTGAAGAT
AAAAATATTATTCCATGCTTCCATTTAACTT----------CAAGTGTAGGTATCTATGG
TATTCAAGGCTCAAAAGCTGGTGCCAATGCAGTTGCTGCTTATTTGAGCCATTCAATTAT
GCGTACATCAAAATCTGGTTATGGTATGATCTTAAAGAGATGTTTATTAAGTACAAGACT
CTTCTACTTGAGATTATTATGGTTATGTGGTGATAATGATCAATTCAAAGTTGTTGGTGT
ACCAATTGAACCATCTAAGGAAACCCTCGATAATCTCAAATCAAGATTATACGATGCCAA
TGGTCAAATGAAGAGTGTTGATACAATTTGTCAAGATCAACAACTCGTCGTTGATTTAGC
TGAAGTTGGACCAGATCAAAATATCGTTTGTTTTGGTATTAATTTCAAGGATGCCAATGG
TAAGTGGAATACTGATTACAACAAATACTGTGATGTTAATAATCAAATCTATGAAAGATT
TGATTATAAAAAAGAAGTTGGTATGGCTCAATTTGATTTAATTTTCTCAAATACCGCTTT
CAACGATCAGTATGGTCAAGTATTCCAACAAAGTCTTGCCCAAAGATTTGGTCTCACTAC
CAATGTTCCAGCTTTTGGTAGTAATAAATTCCAATTACCAGTTTTAAGATCAACTGTTAT
GGATGTCTTTGCTAACGAAACCACTAAAGGTTCATTCTTTGATGTTACAATGCCATTGTT
CAAATCAGAATGCGCTAAAATTGTTGCTGCATTTGTTAACGCTTCCAAGGCTTCAAAACC
AACTCTTTAAATTTTAAATTTAATCAAAATAATAAAAAATATATTTTTTATTTATTCATT
CATTGTATTTTTTTAAATTTTAAAA