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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHD256
Library VH
(Link to library)
Clone ID VHD256
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16508-1
Original site URL
Representative seq. ID VHD256P
(Link to Original site)
Representative DNA sequence 
>VHD256 (VHD256Q) /CSM/VH/VHD2-C/VHD256Q.Seq.d/
CATTATCGGTCGTATGGATTATCAAGATATCGAAGCTCGTTTAGAGAATAAGCAAATGGA
GTTCATGTGGAGATCGACCCAAAGTACACCAGAGAATCAAGTTTTCACATCAGTACTACG
TGCAATGTATTGTACTCCAGATGGTTTCAACTTTGAGCAAGGTGATGACCCAATTCAAGA
TGACCCAAATTTATTCGATAATAACGTAGACTCAAGAGCTGAACAGTTCACTCAAGTGGC
ACTTGAATATGCAACTCATTATCGTACCAATAATGTACTAATTCCATTTGGTTGTGATTT
CGCTTACCTTAACGCTCAAATGTATTACAAAAACATTGACAAACTAATCGCACATATCAA
TTCAAATCCGGATAAATATGGTTTAAATTTGTTATATTCAACTCCATCAATTTATATAGA
TGCTGTAAATGATGCCAATCTAGTATGGGAAGTGAAAACTGATGATCTCTTCCCATATGC
TGATAATGAATTTAGTTATTGGACTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTA
CGTTCGTCAAAATAATGCTCTATTACATGTAGTTGAACAAATGTTGGTAACTAGTAGTAA
TCTTATGCCAAGTAGTAGATCTGAACAATTGGTTGATGACATTGTTATAATGAGAGAGGT
AATGGGTATTGCTCAACATCATGATGCCGTXXXXXXXXXXGCATCGTTAAGAGATGGTCA
ATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACC
AATGAATGAGTCAACTCAAATCGTTACANCTAGTAAATTGATTTTCCATGATATTTCAAG
TTATGCTCAATCTCAGGATCGTCCTGCTGCACTGTNTTTATCACATCCATTACTCCCAAT
GTTTACAACCACTCNGCAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTC
ACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTT
AGATAATCAAGATAATNCCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCA
AGATTCACAAGATCCGCNAACTATCACTTTAGATCTTTCAACTATCTTTTCANCAATTAC
AATCNGCTTCTGCAACTGAAATGAATTTAACTGGTGTTCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
IIGRMDYQDIEARLENKQMEFMWRSTQSTPENQVFTSVLRAMYCTPDGFNFEQGDDPIQD
DPNLFDNNVDSRAEQFTQVALEYATHYRTNNVLIPFGCDFAYLNAQMYYKNIDKLIAHIN
SNPDKYGLNLLYSTPSIYIDAVNDANLVWEVKTDDLFPYADNEFSYWTGYFVSRPALKGY
VRQNNALLHVVEQMLVTSSNLMPSSRSEQLVDDIVIMREVMGIAQHHDA---

---ASLRDGQLDMMMHRRTLKDDGRGVGQPMNESTQIVTXSKLIFHDISSYAQSQDRPAA
LXLSHPLLPMFTTTXQSSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNXILLR
IENIYQIDGQDSQDPXTITLDLSTIFSXITIXFCN*nefnwcs
Translated Amino Acid sequence (All Frames) 
Frame A:
hyrsyglsryrssfre*angvhveidpkytressfhisttcnvlysrwfql*ar**pnsr
*pkfir**rrlks*tvhssgt*icnslsyq*ctnsiwl*frlp*rsnvlqkh*qtnrtyq
fksg*iwfkfvifnsinlyrcck*cqssmgsen**slpic***i*lldwlfr*sssierl
rssk*csitcs*tnvgn***syak**i*tig**hcynergngycsts*cr---

---ASLRDGQLDMMMHRRTLKDDGRGVGQPMNESTQIVTXSKLIFHDISSYAQSQDRPAA
LXLSHPLLPMFTTTXQSSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNXILLR
IENIYQIDGQDSQDPXTITLDLSTIFSXITIXFCN*nefnwcs

Frame B:
IIGRMDYQDIEARLENKQMEFMWRSTQSTPENQVFTSVLRAMYCTPDGFNFEQGDDPIQD
DPNLFDNNVDSRAEQFTQVALEYATHYRTNNVLIPFGCDFAYLNAQMYYKNIDKLIAHIN
SNPDKYGLNLLYSTPSIYIDAVNDANLVWEVKTDDLFPYADNEFSYWTGYFVSRPALKGY
VRQNNALLHVVEQMLVTSSNLMPSSRSEQLVDDIVIMREVMGIAQHHDA---

---hr*emvnwi**civvl*kmmvvvlvnq*msqlkslxlvn*fsmifqvmlnlrivllh
cxyhihysqclqplxnlqtigilnikvfthh*lqphlyqmv*rfklyng*iikixpyfyv
lkifiklmvkihkirxlsl*ifqlsfxqlqsasatemnltgvq

Frame C:
lsvvwiikisklv*riskwsscgdrpkvhqrikfshqyyvqcivlqmvstlskvmtqfkm
tqiysiit*tqelnsslkwhlnmqliivpimy*fhlvvisltltlkcitktltn*shisi
qirinmv*icyiqlhqfi*ml*mmpi*ygk*klmisshmlimnlviglvislvvqh*kat
fvkimlyym*lnkcw*lvvilcqvvdlnnwlmtll**er*wvllnimmp---

---ivkrwsigyddassyfer*wswcwstne*vnsnryx**idfp*yfklcsisgsscct
vfitsitpnvynhsaifkrlefsisrclltidfsltftkwfkdsnstmvr*sr*xhtfty
*kylsn*wsrftrsanyhfrsfnylfxnynxllqlk*i*lvf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD256 (VHD256Q) /CSM/VH/VHD2-C/VHD256Q.Seq.d/                       2240   0.0
VHB425 (VHB425Q) /CSM/VH/VHB4-B/VHB425Q.Seq.d/                       1350   0.0
VHL503 (VHL503Q) /CSM/VH/VHL5-A/VHL503Q.Seq.d/                       1332   0.0
VHI208 (VHI208Q) /CSM/VH/VHI2-A/VHI208Q.Seq.d/                       1322   0.0
CHG632 (CHG632Q) /CSM/CH/CHG6-B/CHG632Q.Seq.d/                       1294   0.0
VHK686 (VHK686Q) /CSM/VH/VHK6-D/VHK686Q.Seq.d/                       1292   0.0
VHL713 (VHL713Q) /CSM/VH/VHL7-A/VHL713Q.Seq.d/                       1291   0.0
SHA382 (SHA382Q) /CSM/SH/SHA3-D/SHA382Q.Seq.d/                       1285   0.0
VHG861 (VHG861Q) /CSM/VH/VHG8-C/VHG861Q.Seq.d/                       1281   0.0
AHI619 (AHI619Q) /CSM/AH/AHI6-A/AHI619Q.Seq.d/                       1281   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1368   0.0    1
BQ999895|BQ999895.1 QGG23F15.yg.ab1 QG_EFGHJ lettuce serriola Lactuca sativa cDNA clone QGG23F15, mRNA sequence.    44   2e-04  2
BQ793980|BQ793980.1 EST 2918 Veraison Grape berries Lambda Zap II Library Vitis vinifera cDNA clone TT251C04 3', mRNA sequence.    40   0.034  2
EL473838|EL473838.1 CHUL1765.b1_I10.ab1 CHU(LMS) puzzle sunflower Helianthus paradoxus cDNA clone CHUL1765, mRNA sequence.    44   0.035  2
BQ793878|BQ793878.1 EST 2816 Veraison Grape berries Lambda Zap II Library Vitis vinifera cDNA clone TT251B04 3', mRNA sequence.    40   0.036  2
AM569193|AM569193.1 Paracentrotus lividus EST, clone MPMGp1173O11114Q, 5' end.    50   0.15   1
DY809519|DY809519.1 CTOX18042.b1_D24.ab1 CTO(XYZ) dandelion Taraxacum officinale cDNA clone CTOX18042, mRNA sequence.    38   0.16   2
CE529546|CE529546.1 tigr-gss-dog-17000365859317 Dog Library Canis familiaris genomic, genomic survey sequence.    48   0.58   1
EG619833|EG619833.1 EMBRYOF088227O8 POSSUM_01-POSSUM-C-EMBRYO-2KB Trichosurus vulpecula cDNA clone 1061028989863, mRNA sequence.    46   2.3    1
DD002107|DD002107.1 Neisseria Genomic Sequences And Methods Of Their Use.    46   2.3    1
dna update 2007. 5.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   474   e-132
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   474   e-132
BC091843_1(BC091843|pid:none) Danio rerio zgc:110815, mRNA (cDNA...   204   4e-51
BC121882_1(BC121882|pid:none) Xenopus tropicalis lysosomal alpha...   189   2e-46
AE014134_1973(AE014134|pid:none) Drosophila melanogaster chromos...   187   8e-46
AY119107_1(AY119107|pid:none) Drosophila melanogaster RE08556 fu...   186   1e-45
AE014134_1633(AE014134|pid:none) Drosophila melanogaster chromos...   186   1e-45
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...   184   4e-45
U40948_5(U40948|pid:none) Caenorhabditis elegans cosmid F55D10, ...   174   5e-42
AB026648_12(AB026648|pid:none) Arabidopsis thaliana genomic DNA,...   174   5e-42
protein update 2009. 6.28
PSORT 

 psg: 0.75  gvh: 0.35  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHD256 is cyt
5' end seq. ID VHD256F
5' end seq. 
>VHD256F.Seq
CATTATCGGTCGTATGGATTATCAAGATATCGAAGCTCGTTTAGAGAATAAGCAAATGGA
GTTCATGTGGAGATCGACCCAAAGTACACCAGAGAATCAAGTTTTCACATCAGTACTACG
TGCAATGTATTGTACTCCAGATGGTTTCAACTTTGAGCAAGGTGATGACCCAATTCAAGA
TGACCCAAATTTATTCGATAATAACGTAGACTCAAGAGCTGAACAGTTCACTCAAGTGGC
ACTTGAATATGCAACTCATTATCGTACCAATAATGTACTAATTCCATTTGGTTGTGATTT
CGCTTACCTTAACGCTCAAATGTATTACAAAAACATTGACAAACTAATCGCACATATCAA
TTCAAATCCGGATAAATATGGTTTAAATTTGTTATATTCAACTCCATCAATTTATATAGA
TGCTGTAAATGATGCCAATCTAGTATGGGAAGTGAAAACTGATGATCTCTTCCCATATGC
TGATAATGAATTTAGTTATTGGACTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTA
CGTTCGTCAAAATAATGCTCTATTACATGTAGTTGAACAAATGTTGGTAACTAGTAGTAA
TCTTATGCCAAGTAGTAGATCTGAACAATTGGTTGATGACATTGTTATAATGAGAGAGGT
AATGGGTATTGCTCAACATCATGATGCCGTNNNNNNNNNN
Length of 5' end seq. 700
3' end seq. ID VHD256Z
3' end seq. 
>VHD256Z.Seq
NNNNNNNNNNGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTT
GAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACANC
TAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCAGGATCGTCCTGCTGC
ACTGTNTTTATCACATCCATTACTCCCAATGTTTACAACCACTCNGCAATCTTCAAACGA
TTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAA
TGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATNCCATACTTTTACG
TATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCGCNAACTATCACTTT
AGATCTTTCAACTATCTTTTCANCAATTACAATCNGCTTCTGCAACTGAAATGAATTTAA
CTGGTGTTCAA
Length of 3' end seq. 491
Connected seq. ID VHD256P
Connected seq. 
>VHD256P.Seq
CATTATCGGTCGTATGGATTATCAAGATATCGAAGCTCGTTTAGAGAATAAGCAAATGGA
GTTCATGTGGAGATCGACCCAAAGTACACCAGAGAATCAAGTTTTCACATCAGTACTACG
TGCAATGTATTGTACTCCAGATGGTTTCAACTTTGAGCAAGGTGATGACCCAATTCAAGA
TGACCCAAATTTATTCGATAATAACGTAGACTCAAGAGCTGAACAGTTCACTCAAGTGGC
ACTTGAATATGCAACTCATTATCGTACCAATAATGTACTAATTCCATTTGGTTGTGATTT
CGCTTACCTTAACGCTCAAATGTATTACAAAAACATTGACAAACTAATCGCACATATCAA
TTCAAATCCGGATAAATATGGTTTAAATTTGTTATATTCAACTCCATCAATTTATATAGA
TGCTGTAAATGATGCCAATCTAGTATGGGAAGTGAAAACTGATGATCTCTTCCCATATGC
TGATAATGAATTTAGTTATTGGACTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTA
CGTTCGTCAAAATAATGCTCTATTACATGTAGTTGAACAAATGTTGGTAACTAGTAGTAA
TCTTATGCCAAGTAGTAGATCTGAACAATTGGTTGATGACATTGTTATAATGAGAGAGGT
AATGGGTATTGCTCAACATCATGATGCCGT----------GCATCGTTAAGAGATGGTCA
ATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACC
AATGAATGAGTCAACTCAAATCGTTACANCTAGTAAATTGATTTTCCATGATATTTCAAG
TTATGCTCAATCTCAGGATCGTCCTGCTGCACTGTNTTTATCACATCCATTACTCCCAAT
GTTTACAACCACTCNGCAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTC
ACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTT
AGATAATCAAGATAATNCCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCA
AGATTCACAAGATCCGCNAACTATCACTTTAGATCTTTCAACTATCTTTTCANCAATTAC
AATCNGCTTCTGCAACTGAAATGAATTTAACTGGTGTTCAA
Length of connected seq. 1171
Full length Seq ID -
Full length Seq. -
Length of full length seq. -