>VHD252 (VHD252Q) /CSM/VH/VHD2-C/VHD252Q.Seq.d/
ATTTCACTTGTTTACTTTAATTTTATACATATAAATAATAATATAGTTGAAAAAATGTGT
GATCATAAACATTTAGTTGACAAAGTAGATAGATTAGTATTACCAGATAATATCGTACCA
AGTAAATACGATTTACATCTTAGACCAAATTTAAAGGATTTTGTATTCAGCGGCAAAGTT
GACATTACAATCAATATTGTTAAACCAACCAAAAAAATCATTATCCATTCAATTGACATT
GAAATTCAATCAGTAAAGATCTTAAATCAACAAGCTACTTCCGTTACCTACTATGAACCA
GAAGAAGTTGCAATTTTAGAATTCCAAGATGAATTACCAGTTACAGAAAATACAATTCTC
TCCATTGATTTCACTGGTATTTTAAATGACAAATTAAAAGGTTTCTATCGTTCAAAATAT
GTTGTCAATGGTGAAGATCGTTATATTGGTACCACTCAATTCGAAGCCACTGATGCTCGT
CGTGCTTTCCCATGTTTTGATGAACCTGCTTTAAAATCTTTCTTTAATATTAAAATCACT
ATTTCATCTCATTTAACTGCTCTTTCAAATATGGATACAACTTCTGXXXXXXXXXXTATT
CAAACCTTTAATTGAAGATCANCCATACTCAAGTAGATTCAATGAAATCTTTGTTCAACT
TTTGAAACCACTTTCAAAACGTTTAGGTTTCGAGCCAACCAAAGGTGAACCATCTTCAGA
TACCTTGTTAAGAGATAAAGTAAACACTAAATTGGGTATGTTGGGTGATGCTGATATCGT
TGCTGAATGTAAGAAAAGATTCGAAGCTTTCAAAGCAGACTCTTCAAGTTTACCATCAGA
TATTCGTTCTACAGTACTTGCTACAATCGTAAAGAATGGTGGTGAAGCTGATCAACAAAT
CATCATTGATCAATATCTTAAATCTTCTTTAGTCGCTGAAAAGAATTCTTACCTTTTGGT
ACTTGCTTTGGCTCCAAAAGAGGAATTGGTTGAAAAAGCCCTCAATTTCGCTTTATCTCC
ATCCGTTAGAAGTCAAGATAGTTATATGGTTTTCTTTACCCTCCCAAATCGTGTTCGTCA
ATTCGCTTGGGCTTACCTCACCAAAAACTTTAATCAAATCAACGAAACATTTAAAAACTC
TCCTTTGTTTGGTCGTATCATTGGTAGTTGTCTCACCTCTAAAATGGATGATTCTCAATA
CAATGAAGTCGTCAACTTTTTCAAAGATCATCCAGTTCCAATTGCTGATCGTTCAATTAA
ACAAGATTTAGAAATGGTTACAATTAATTCAAATGGTTCAAAGCTTTTAATCAAGATTTA
TCAAAT

ISLVYFNFIHINNNIVEKMCDHKHLVDKVDRLVLPDNIVPSKYDLHLRPNLKDFVFSGKV
DITINIVKPTKKIIIHSIDIEIQSVKILNQQATSVTYYEPEEVAILEFQDELPVTENTIL
SIDFTGILNDKLKGFYRSKYVVNGEDRYIGTTQFEATDARRAFPCFDEPALKSFFNIKIT
ISSHLTALSNMDTTS---

---FKPLIEDXPYSSRFNEIFVQLLKPLSKRLGFEPTKGEPSSDTLLRDKVNTKLGMLGD
ADIVAECKKRFEAFKADSSSLPSDIRSTVLATIVKNGGEADQQIIIDQYLKSSLVAEKNS
YLLVLALAPKEELVEKALNFALSPSVRSQDSYMVFFTLPNRVRQFAWAYLTKNFNQINET
FKNSPLFGRIIGSCLTSKMDDSQYNEVVNFFKDHPVPIADRSIKQDLEMVTINSNGSKLL
IKIYQ

Frame A:
ISLVYFNFIHINNNIVEKMCDHKHLVDKVDRLVLPDNIVPSKYDLHLRPNLKDFVFSGKV
DITINIVKPTKKIIIHSIDIEIQSVKILNQQATSVTYYEPEEVAILEFQDELPVTENTIL
SIDFTGILNDKLKGFYRSKYVVNGEDRYIGTTQFEATDARRAFPCFDEPALKSFFNIKIT
ISSHLTALSNMDTTS---

---ysnl*lkixhtqvdsmkslfnf*nhfqnv*vssqpkvnhlqipc*eik*tlnwvcwv
mlisllnvrkdsklskqtlqvyhqifvlqyllqs*rmvvklinksslinilnll*slkri
ltfwyllwlqkrnwlkkpsislylhplevkiviwfslpsqivfvnslgltspktlikstk
hlktllclvvslvvvsplkwmilntmksstfskiiqfqllivqlnki*kwlqliqmvqsf
*srfik

Frame B:
fhlftlilyi*iii*lkkcviini*ltk*id*yyqiisyqvntiyildqi*rilysaakl
tlqsillnqpkkslsiqltlkfnq*rs*inkllplptmnqkklqf*nskmnyqlqkiqfs
plislvf*mtn*kvsivqnmlsmvkivilvplnskplmlvvlshvlmnll*nlslilksl
fhli*llfqiwiqll---

---iqtfn*rsxilk*iq*nlcstfettfktfrfranqr*tifrylvkr*skh*igyvg*
c*yrc*m*ekirsfqsrlfkftirysfystcynrkeww*s*stnhh*sis*iffsr*kef
lpfgtcfgskrgig*kspqfrfisir*ksr*lygflyppkscssirlglphqkl*snqrn
i*klsfvwsyhw*lshl*ng*fsiq*srqlfqrssssnc*sfn*trfrngyn*fkwfkaf
nqdlsn

Frame C:
ftcll*fytyk**ys*knv*s*tfs*qsr*isitr*yrtk*irfts*tkfkgfciqrqs*
hynqyc*tnqknhypfn*h*nsiskdlkstsyfryll*trrscnfripr*itsyrkynsl
h*fhwyfk*qikrflsfkiccqw*rslywyhsirsh*csscfpmf**tcfkifl*y*nhy
fisfncsfkygynf---

---FKPLIEDXPYSSRFNEIFVQLLKPLSKRLGFEPTKGEPSSDTLLRDKVNTKLGMLGD
ADIVAECKKRFEAFKADSSSLPSDIRSTVLATIVKNGGEADQQIIIDQYLKSSLVAEKNS
YLLVLALAPKEELVEKALNFALSPSVRSQDSYMVFFTLPNRVRQFAWAYLTKNFNQINET
FKNSPLFGRIIGSCLTSKMDDSQYNEVVNFFKDHPVPIADRSIKQDLEMVTINSNGSKLL
IKIYQ

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD252 (VHD252Q) /CSM/VH/VHD2-C/VHD252Q.Seq.d/                       2522   0.0
VHI248 (VHI248Q) /CSM/VH/VHI2-B/VHI248Q.Seq.d/                       1425   0.0
VHP808 (VHP808Q) /CSM/VH/VHP8-A/VHP808Q.Seq.d/                       1411   0.0
VHC369 (VHC369Q) /CSM/VH/VHC3-C/VHC369Q.Seq.d/                       1411   0.0
VHI595 (VHI595Q) /CSM/VH/VHI5-D/VHI595Q.Seq.d/                       1409   0.0
VHP126 (VHP126Q) /CSM/VH/VHP1-B/VHP126Q.Seq.d/                       1405   0.0
VHI217 (VHI217Q) /CSM/VH/VHI2-A/VHI217Q.Seq.d/                       1405   0.0
AHB710 (AHB710Q) /CSM/AH/AHB7-A/AHB710Q.Seq.d/                       1398   0.0
AHA345 (AHA345Q) /CSM/AH/AHA3-B/AHA345Q.Seq.d/                       1392   0.0
AHK448 (AHK448Q) /CSM/AH/AHK4-B/AHK448Q.Seq.d/                       1388   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY631393|AY631393.1 Dictyostelium discoideum puromycin-sensitive aminopeptidase-like protein (psa) mRNA, partial cds.    80   4e-19  3
CR382136|CR382136.1 Debaryomyces hansenii chromosome D of strain CBS767 of Debaryomyces hansenii.    56   0.003  1
CP000498|CP000498.1 Pichia stipitis CBS 6054 chromosome 4, complete sequence.    38   0.006  2
CR382123|CR382123.1 Kluyveromyces lactis strain NRRL Y-1140 chromosome C of strain NRRL  Y-1140 of Kluyveromyces lactis.    54   0.010  1
DN815134|AF148323_1 aminopeptidase PILS [Rattus norvegicus], mRNA sequence.    52   0.041  1
BP514490|BP514490.1 Hydra magnipapillata cDNA, clone:hydmg018bw_14.    52   0.041  1
DN573775|DN573775.1 92243740 Sea Urchin primary mesenchyme cell cDNA library Strongylocentrotus purpuratus cDNA clone PMCSPR2-190G18 3', mRNA sequence.    48   0.65   1
CB564189|CB564189.1 AGENCOURT_13329805 NICHD_XGC_Tad2 Xenopus laevis cDNA clone IMAGE:6875222 3', mRNA sequence.    48   0.65   1
T65151|T65151.1 yc74f10.s1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE:21570 3', mRNA sequence.    48   0.65   1
F09498|F09498.1 H. sapiens partial cDNA sequence; clone c-32d10.    48   0.65   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY631393_1(AY631393|pid:none) Dictyostelium discoideum puromycin...   229   6e-59
AF327435_1(AF327435|pid:none) Drosophila melanogaster puromycin-...   148   4e-34
AE014296_282(AE014296|pid:none) Drosophila melanogaster chromoso...   148   5e-34
AE014296_281(AE014296|pid:none) Drosophila melanogaster chromoso...   148   5e-34
AM270075_67(AM270075|pid:none) Aspergillus niger contig An04c014...   132   4e-29
AP007157_574(AP007157|pid:none) Aspergillus oryzae RIB40 genomic...   129   2e-28
AP005572_11(AP005572|pid:none) Oryza sativa Japonica Group genom...   127   7e-28
EF678434_1(EF678434|pid:none) Picea sitchensis clone WS02921_A14...   127   9e-28
EF676179_1(EF676179|pid:none) Picea sitchensis clone WS02717_G19...   126   2e-27
AF350242_1(AF350242|pid:none) Pichia angusta aminopeptidase gene...   125   3e-27

 psg: 0.87  gvh: 0.34  alm: 0.44  top: 0.57  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHD252 is cyt

>VHD252F.Seq
ATTTCACTTGTTTACTTTAATTTTATACATATAAATAATAATATAGTTGAAAAAATGTGT
GATCATAAACATTTAGTTGACAAAGTAGATAGATTAGTATTACCAGATAATATCGTACCA
AGTAAATACGATTTACATCTTAGACCAAATTTAAAGGATTTTGTATTCAGCGGCAAAGTT
GACATTACAATCAATATTGTTAAACCAACCAAAAAAATCATTATCCATTCAATTGACATT
GAAATTCAATCAGTAAAGATCTTAAATCAACAAGCTACTTCCGTTACCTACTATGAACCA
GAAGAAGTTGCAATTTTAGAATTCCAAGATGAATTACCAGTTACAGAAAATACAATTCTC
TCCATTGATTTCACTGGTATTTTAAATGACAAATTAAAAGGTTTCTATCGTTCAAAATAT
GTTGTCAATGGTGAAGATCGTTATATTGGTACCACTCAATTCGAAGCCACTGATGCTCGT
CGTGCTTTCCCATGTTTTGATGAACCTGCTTTAAAATCTTTCTTTAATATTAAAATCACT
ATTTCATCTCATTTAACTGCTCTTTCAAATATGGATACAACTTCTGNNNNNNNNNN

>VHD252Z.Seq
NNNNNNNNNNTATTCAAACCTTTAATTGAAGATCANCCATACTCAAGTAGATTCAATGAA
ATCTTTGTTCAACTTTTGAAACCACTTTCAAAACGTTTAGGTTTCGAGCCAACCAAAGGT
GAACCATCTTCAGATACCTTGTTAAGAGATAAAGTAAACACTAAATTGGGTATGTTGGGT
GATGCTGATATCGTTGCTGAATGTAAGAAAAGATTCGAAGCTTTCAAAGCAGACTCTTCA
AGTTTACCATCAGATATTCGTTCTACAGTACTTGCTACAATCGTAAAGAATGGTGGTGAA
GCTGATCAACAAATCATCATTGATCAATATCTTAAATCTTCTTTAGTCGCTGAAAAGAAT
TCTTACCTTTTGGTACTTGCTTTGGCTCCAAAAGAGGAATTGGTTGAAAAAGCCCTCAAT
TTCGCTTTATCTCCATCCGTTAGAAGTCAAGATAGTTATATGGTTTTCTTTACCCTCCCA
AATCGTGTTCGTCAATTCGCTTGGGCTTACCTCACCAAAAACTTTAATCAAATCAACGAA
ACATTTAAAAACTCTCCTTTGTTTGGTCGTATCATTGGTAGTTGTCTCACCTCTAAAATG
GATGATTCTCAATACAATGAAGTCGTCAACTTTTTCAAAGATCATCCAGTTCCAATTGCT
GATCGTTCAATTAAACAAGATTTAGAAATGGTTACAATTAATTCAAATGGTTCAAAGCTT
TTAATCAAGATTTATCAAAT

>VHD252P.Seq
ATTTCACTTGTTTACTTTAATTTTATACATATAAATAATAATATAGTTGAAAAAATGTGT
GATCATAAACATTTAGTTGACAAAGTAGATAGATTAGTATTACCAGATAATATCGTACCA
AGTAAATACGATTTACATCTTAGACCAAATTTAAAGGATTTTGTATTCAGCGGCAAAGTT
GACATTACAATCAATATTGTTAAACCAACCAAAAAAATCATTATCCATTCAATTGACATT
GAAATTCAATCAGTAAAGATCTTAAATCAACAAGCTACTTCCGTTACCTACTATGAACCA
GAAGAAGTTGCAATTTTAGAATTCCAAGATGAATTACCAGTTACAGAAAATACAATTCTC
TCCATTGATTTCACTGGTATTTTAAATGACAAATTAAAAGGTTTCTATCGTTCAAAATAT
GTTGTCAATGGTGAAGATCGTTATATTGGTACCACTCAATTCGAAGCCACTGATGCTCGT
CGTGCTTTCCCATGTTTTGATGAACCTGCTTTAAAATCTTTCTTTAATATTAAAATCACT
ATTTCATCTCATTTAACTGCTCTTTCAAATATGGATACAACTTCTG----------TATT
CAAACCTTTAATTGAAGATCANCCATACTCAAGTAGATTCAATGAAATCTTTGTTCAACT
TTTGAAACCACTTTCAAAACGTTTAGGTTTCGAGCCAACCAAAGGTGAACCATCTTCAGA
TACCTTGTTAAGAGATAAAGTAAACACTAAATTGGGTATGTTGGGTGATGCTGATATCGT
TGCTGAATGTAAGAAAAGATTCGAAGCTTTCAAAGCAGACTCTTCAAGTTTACCATCAGA
TATTCGTTCTACAGTACTTGCTACAATCGTAAAGAATGGTGGTGAAGCTGATCAACAAAT
CATCATTGATCAATATCTTAAATCTTCTTTAGTCGCTGAAAAGAATTCTTACCTTTTGGT
ACTTGCTTTGGCTCCAAAAGAGGAATTGGTTGAAAAAGCCCTCAATTTCGCTTTATCTCC
ATCCGTTAGAAGTCAAGATAGTTATATGGTTTTCTTTACCCTCCCAAATCGTGTTCGTCA
ATTCGCTTGGGCTTACCTCACCAAAAACTTTAATCAAATCAACGAAACATTTAAAAACTC
TCCTTTGTTTGGTCGTATCATTGGTAGTTGTCTCACCTCTAAAATGGATGATTCTCAATA
CAATGAAGTCGTCAACTTTTTCAAAGATCATCCAGTTCCAATTGCTGATCGTTCAATTAA
ACAAGATTTAGAAATGGTTACAATTAATTCAAATGGTTCAAAGCTTTTAATCAAGATTTA
TCAAAT