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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHD237
Library VH
(Link to library)
Clone ID VHD237
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHD237P
(Link to Original site)
Representative DNA sequence 
>VHD237 (VHD237Q) /CSM/VH/VHD2-B/VHD237Q.Seq.d/
GAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTCGTGCCAT
TATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATCACGGTAA
AACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAGTTTCTGG
TGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCATTAAATC
TTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTGGTTGTAC
TTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAGTTCAGA
AGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGAAGG
TGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAAACCAGT
CTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTGAAGAAGC
TTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTXXXXXXXXXXTCTCG
CTGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAG
GTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTA
AAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAA
ACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACA
GAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTG
CCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTA
TTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTC
GTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTT
TCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATC
ATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCA
CCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAAC
TCTAAACAATTTAAGTCGCACATNAGTTTTCAAATATAGAATCAAATCTAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
SAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKVSG
DMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFSSE
VTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTEEA
YLSFRRAIESVNVI---

---LADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGV
VCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITA
PENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQ
CVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*vahxfsniesn
lk
Translated Amino Acid sequence (All Frames) 
Frame A:
eckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*sfw
*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fqfr
snccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*rs
lslfp*sy*irqchc---

---sr**prmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rffrwcfpmghqrrc
cl**kherypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpnslrtnllsrnyc
prkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfpt
mclrslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlsrtxvfkyrik
skk

Frame B:
SAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKVSG
DMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFSSE
VTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTEEA
YLSFRRAIESVNVI---

---LADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGV
VCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITA
PENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQ
CVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*vahxfsniesn
lk

Frame C:
vqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpikflv
i*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlisvqk
*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tlkkl
islsvellnpsmsl---

---slittngikmmp*tfglsvqkvtvpisssmspkvfni*mklkilslvlsngppkkvl
fvmkt*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppqls*nqst**kllp
qktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspliyvptlpvklshn
vssiiglqlvlstkirkplksl*pperekv*pqksqiltnstknskqfkshxsfqi*nqi
*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD237 (VHD237Q) /CSM/VH/VHD2-B/VHD237Q.Seq.d/                       2516   0.0
VHQ293 (VHQ293Q) /CSM/VH/VHQ2-D/VHQ293Q.Seq.d/                       1407   0.0
VHP730 (VHP730Q) /CSM/VH/VHP7-B/VHP730Q.Seq.d/                       1407   0.0
VHP405 (VHP405Q) /CSM/VH/VHP4-A/VHP405Q.Seq.d/                       1407   0.0
VHP357 (VHP357Q) /CSM/VH/VHP3-C/VHP357Q.Seq.d/                       1407   0.0
VHN889 (VHN889Q) /CSM/VH/VHN8-D/VHN889Q.Seq.d/                       1407   0.0
VHN855 (VHN855Q) /CSM/VH/VHN8-C/VHN855Q.Seq.d/                       1407   0.0
VHK894 (VHK894Q) /CSM/VH/VHK8-D/VHK894Q.Seq.d/                       1407   0.0
VHK419 (VHK419Q) /CSM/VH/VHK4-A/VHK419Q.Seq.d/                       1407   0.0
VHK162 (VHK162Q) /CSM/VH/VHK1-C/VHK162Q.Seq.d/                       1407   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1257   0.0    4
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    72   5e-71  13
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   1e-70  9
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   3e-69  9
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    70   5e-66  10
AF107290|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds.    64   5e-49  8
AF534908|AF534908.2 Tetrahymena thermophila elongation factor 2 gene, complete cds.    84   3e-47  6
AY144999|AY144999.1 Saccharomyces kluyveri clone Contig1706 EFT2 gene, complete cds.    78   3e-45  7
AR550596|AR550596.1 Sequence 5727 from patent US 6747137.          78   2e-44  6
EH015431|EH015431.1 USDA-FP_188032 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt081_G08 5', mRNA sequence.    78   6e-43  4
dna update 2007. 5.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   291   3e-77
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   290   1e-76
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   289   2e-76
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   289   2e-76
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   288   3e-76
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   288   4e-76
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   287   7e-76
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   285   3e-75
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   285   3e-75
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...   285   3e-75
protein update 2009. 6.28
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHD237 is cyt
5' end seq. ID VHD237F
5' end seq. 
>VHD237F.Seq
GAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTCGTGCCAT
TATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATCACGGTAA
AACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAGTTTCTGG
TGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCATTAAATC
TTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTGGTTGTAC
TTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAGTTCAGA
AGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGAAGG
TGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAAACCAGT
CTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTGAAGAAGC
TTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTNNNNNNNNNN
Length of 5' end seq. 595
3' end seq. ID VHD237Z
3' end seq. 
>VHD237Z.Seq
NNNNNNNNNNTCTCGCTGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGT
CTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAAT
ATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTG
TTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACA
CTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATG
CTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTG
CCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTA
TTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTAT
TAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCAC
AATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTG
AAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACA
AATTCCACGAAAAACTCTAAACAATTTAAGTCGCACATNAGTTTTCAAATATAGAATCAA
ATCTAAAAAA
Length of 3' end seq. 730
Connected seq. ID VHD237P
Connected seq. 
>VHD237P.Seq
GAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTCGTGCCAT
TATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATCACGGTAA
AACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAGTTTCTGG
TGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCATTAAATC
TTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTGGTTGTAC
TTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAGTTCAGA
AGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGAAGG
TGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAAACCAGT
CTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTGAAGAAGC
TTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGT----------TCTCG
CTGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAG
GTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTA
AAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAA
ACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACA
GAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTG
CCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTA
TTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTC
GTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTT
TCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATC
ATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCA
CCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAAC
TCTAAACAATTTAAGTCGCACATNAGTTTTCAAATATAGAATCAAATCTAAAAAA
Length of connected seq. 1305
Full length Seq ID -
Full length Seq. -
Length of full length seq. -