>VHD186 (VHD186Q) /CSM/VH/VHD1-D/VHD186Q.Seq.d/
XXXXXXXXXXTCCGATCAATTAGGTTTTCCAAAGACTACCAATGAAGTTCAATTTGGTCA
AAAATTACCATTAATTGAAATCCCATCAAATAATATCGCTCGTATCTTCTTTAGAATCGC
ATNTGAAGCTCAACCATATCAAGCTCAACAAGTTGGTATTCGTTTCTATTCACCAGCTAG
AGAAGCCGTAGTTCCAGCAACTTACTCTGCTGATGCCTACTCTTACACCTTCACCAATAA
AGATGCTTGCAAGATTTTAGGTTGTCAATCTGGAAATTATCAATTGGATTTAATCATTGG
TGATCAATCAATCACTCCATTACAATGGAATTTCGGTGAAATTAATTTGAAATTCAATCA
ATCAACCATTCCAACCAATCGTTATCCAGAACAACTCCCAATCTCTCACAACTTTAGAGT
AGCTGAAAAGAGACCACCACAATCTATCTCAAGTTTATTCACTCTCTTGGTCTTATCACC
AATCGCCATCTTTGTCATTGGTCTCTTATTCGTTGGTACCAATCTTGGTAGATTCCCAAC
TGGTATGGGTTTCATTTACACCATTGGTTTCCTTGGTTGTATCTCTGCAACTGGTCTCTT
AATTGTAAACTATTGGTTACACTT

---DQLGFPKTTNEVQFGQKLPLIEIPSNNIARIFFRIAXEAQPYQAQQVGIRFYSPARE
AVVPATYSADAYSYTFTNKDACKILGCQSGNYQLDLIIGDQSITPLQWNFGEINLKFNQS
TIPTNRYPEQLPISHNFRVAEKRPPQSISSLFTLLVLSPIAIFVIGLLFVGTNLGRFPTG
MGFIYTIGFLGCISATGLLIVNYWLH

Frame A:
---pin*vfqrlpmkfnlvknyh*lkshqiislvssleshxklnhiklnklvfvsihqle
kp*fqqltllmptltpspikmlarf*vvnleiinwi*slvinqslhyngisvkli*nsin
qpfqpiviqnnsqslttle*lkrdhhnlsqvyslswsyhqspslslvsyslvpilvdsql
vwvsftplvslvvslqlvs*l*tigyt


Frame B:
---rsirfskdyq*ssiwskitin*npik*yrsyll*nrx*sstissstswysflfts*r
srsssnllc*clllhlhq*rclqdfrlsiwklsigfnhw*sinhsitmefr*n*feiqsi
nhsnqslsrttpnlsql*ss*ketttiylkfihslglitnrhlchwslirwyqsw*ipnw
ygfhlhhwfpwlylcnwslnckllvtl


Frame C:
---DQLGFPKTTNEVQFGQKLPLIEIPSNNIARIFFRIAXEAQPYQAQQVGIRFYSPARE
AVVPATYSADAYSYTFTNKDACKILGCQSGNYQLDLIIGDQSITPLQWNFGEINLKFNQS
TIPTNRYPEQLPISHNFRVAEKRPPQSISSLFTLLVLSPIAIFVIGLLFVGTNLGRFPTG
MGFIYTIGFLGCISATGLLIVNYWLH

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD186 (VHD186Q) /CSM/VH/VHD1-D/VHD186Q.Seq.d/                       1211   0.0
VHQ120 (VHQ120Q) /CSM/VH/VHQ1-A/VHQ120Q.Seq.d/                       1201   0.0
VHP310 (VHP310Q) /CSM/VH/VHP3-A/VHP310Q.Seq.d/                       1201   0.0
VHD225 (VHD225Q) /CSM/VH/VHD2-B/VHD225Q.Seq.d/                       1201   0.0
VHC739 (VHC739Q) /CSM/VH/VHC7-B/VHC739Q.Seq.d/                       1201   0.0
AFN258 (AFN258Q) /CSM/AF/AFN2-C/AFN258Q.Seq.d/                       1201   0.0
VHO870 (VHO870Q) /CSM/VH/VHO8-C/VHO870Q.Seq.d/                       1193   0.0
VHC534 (VHC534Q) /CSM/VH/VHC5-B/VHC534Q.Seq.d/                       1193   0.0
VHE188 (VHE188Q) /CSM/VH/VHE1-D/VHE188Q.Seq.d/                       1179   0.0
VHM302 (VHM302Q) /CSM/VH/VHM3-A/VHM302Q.Seq.d/                       1176   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CR339063|CR339063.8 Zebrafish DNA sequence from clone CH211-247J10 in linkage group 20.    44   0.003  2
AC176336|AC176336.1 Strongylocentrotus purpuratus clone R3-3053M2, WORKING DRAFT SEQUENCE, 8 unordered pieces.    36   0.17   2
AC005593|AC005593.1 Homo sapiens chromosome 5, P1 clone 1369f10 (LBNL H28), complete sequence.    46   0.86   2
BQ005980|BQ005980.1 UI-H-EI1-ayy-f-17-0-UI.s1 NCI_CGAP_EI1 Homo sapiens cDNA clone IMAGE:5845264 3', mRNA sequence.    46   1.2    1
AC182521|AC182521.2 Callithrix jacchus clone CH259-180D18, WORKING DRAFT SEQUENCE, 4 ordered pieces.    46   1.2    1
DE982219|DE982219.1 Macropus eugenii DNA, BAC clone: MEB1-191G04_F, 5'end.    46   1.2    1
DE975040|DE975040.1 Macropus eugenii DNA, BAC clone: MEB1-181J24_F, 5'end.    46   1.2    1
AC099772|AC099772.3 Mus Musculus Chromosome 5 RP23-319P12 Strain C57Bl6/J, complete sequence.    46   1.2    1
AC184640|AC184640.1 Strongylocentrotus purpuratus clone R3-3088D16, WORKING DRAFT SEQUENCE, 23 unordered pieces.    32   2.3    2
CT737186|CT737186.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-271J14.    34   2.3    2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9GL01) RecName: Full=Dolichyl-diphosphooligosaccharide--protei...    72   2e-11
AK096243_1(AK096243|pid:none) Homo sapiens cDNA FLJ38924 fis, cl...    71   3e-11
(P04844) RecName: Full=Dolichyl-diphosphooligosaccharide--protei...    71   3e-11
DQ895552_1(DQ895552|pid:none) Synthetic construct Homo sapiens c...    71   3e-11
B26168(B26168)ribophorin II precursor - human                          71   3e-11
Y00282_1(Y00282|pid:none) Human mRNA for ribophorin II.                71   3e-11
BC002380_1(BC002380|pid:none) Homo sapiens ribophorin II, mRNA (...    71   3e-11
CR860502_1(CR860502|pid:none) Pongo abelii mRNA; cDNA DKFZp468G2...    70   4e-11
(P25235) RecName: Full=Dolichyl-diphosphooligosaccharide--protei...    70   4e-11
AK315801_1(AK315801|pid:none) Homo sapiens cDNA, FLJ96923, highl...    70   5e-11

 psg: 0.75  gvh: 0.43  alm: 0.15  top: 0.50  tms: 0.13  mit: 0.09  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: endoplasmic reticulum
	 28.0 %: plasma membrane
	 16.0 %: mitochondrial
	  8.0 %: extracellular, including cell wall
	  4.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VHD186 is end

>VHD186Z.Seq
NNNNNNNNNNTCCGATCAATTAGGTTTTCCAAAGACTACCAATGAAGTTCAATTTGGTCA
AAAATTACCATTAATTGAAATCCCATCAAATAATATCGCTCGTATCTTCTTTAGAATCGC
ATNTGAAGCTCAACCATATCAAGCTCAACAAGTTGGTATTCGTTTCTATTCACCAGCTAG
AGAAGCCGTAGTTCCAGCAACTTACTCTGCTGATGCCTACTCTTACACCTTCACCAATAA
AGATGCTTGCAAGATTTTAGGTTGTCAATCTGGAAATTATCAATTGGATTTAATCATTGG
TGATCAATCAATCACTCCATTACAATGGAATTTCGGTGAAATTAATTTGAAATTCAATCA
ATCAACCATTCCAACCAATCGTTATCCAGAACAACTCCCAATCTCTCACAACTTTAGAGT
AGCTGAAAAGAGACCACCACAATCTATCTCAAGTTTATTCACTCTCTTGGTCTTATCACC
AATCGCCATCTTTGTCATTGGTCTCTTATTCGTTGGTACCAATCTTGGTAGATTCCCAAC
TGGTATGGGTTTCATTTACACCATTGGTTTCCTTGGTTGTATCTCTGCAACTGGTCTCTT
AATTGTAAACTATTGGTTACACTT