>VHD153 (VHD153Q) /CSM/VH/VHD1-C/VHD153Q.Seq.d/
ATTTTAGTCAATCAATATTGTGTGTATATGTATAATAATTGTGTATATTAGTGTATAATA
TATATCTATTTTTAAACAGATCAACAATAAGAAAAAAATGAAAAGATCAGAAAAATCAAG
TACATCTGTTGTTAGTAATAACAAACAACAAGATGTAAATATCATCAGTTCAAATGAAGT
TGGTGTTAAAGAAGAAAATAAAGGACATCAAGAATTCTTATTAAAAGTTTTAATTCTATC
AGTCATTTATGTTTTAGCATTTTCAACTCGTTTATTCTCAGTATTACGTTATGAAAGTGT
TATTCATGAATTTGATCCATATTTTAATTATAGATCAACAATATATCTTGTTCAAGAAGG
TTTTTATAATTTTTTAAATTGGTTTGATGAAAGAGCATGGTATCCATTAGGACGTATTGT
AGGTGGTACAATTTACCCAGGTTAATGGCAACAGCAAGTTTAGTTCATTGGTCATTGAAT
TCATTGAATATTACAGTXXXXXXXXXXTCCATCCATTGTACTCTCTGCCAAACAAAACGA
TGGTAGTCGTGTGATTTTCGATGGTTTCCGTGAAGCCTACCGTTGGATTGGTCAAAATAC
TGCCGACGACGCTCGTATTATGTCTTGGTGGGATTATGGTTATCAATTATCTGCAATGGC
CAATCGTACCGTATTGGTTGATAATAACACTTGGAACAATAGTCATATCGCTCAAGTTGG
TAAAGCATTTGCATCCACTGAAGAAGATGCTTACATACAAATGAAAGCATTGGATGTCGA
TTATGTTTTAGTTATTTTTGGTGGTTTAACTGGTTACAGTTCTGATGATATCAATAAATT
CCTTTGGATGGTTAGAATTGGTGGTAGTTGTGATCCAAATATTAAAGAACAAGATTATCT
CACCAATGGTCAATATAGAATAGATAAAGGTGCCTCACCAACAATGTTAAATTCTCTCAT
GTACAAACTTAGTTACTATCGTTTCTCTGAAGTTCACACTGACTATCAAAGACCAACAGG
TTTCGATCGTGTAAGAAATGTTGAAATTGGTAATAAAAATTTCGATTTAACTTATTTAGA
AGAAGCTTTCACATCTGTTCATGGTTAGTTAGAGTTTATAAAGTTAAAGATTTNNATAAT
AGAAG

ilvnqycvymynncvy*ciiyiyf*tdqq*eknekirkikyicc***qttrckyhqfk*s
wc*rrk*rtsriliksfnsishlcfsifnsfilsitl*kcys*i*sif*l*inniscsrr
fl*ffklv**KSMVSIRTYCRWYNLPRLMATASLVHWSLNSLNIT---

---PSIVLSAKQNDGSRVIFDGFREAYRWIGQNTADDARIMSWWDYGYQLSAMANRTVLV
DNNTWNNSHIAQVGKAFASTEEDAYIQMKALDVDYVLVIFGGLTGYSSDDINKFLWMVRI
GGSCDPNIKEQDYLTNGQYRIDKGASPTMLNSLMYKLSYYRFSEVHTDYQRPTGFDRVRN
VEIGNKNFDLTYLEEAFTSVHG*lefiklkixiie

Frame A:
ilvnqycvymynncvy*ciiyiyf*tdqq*eknekirkikyicc***qttrckyhqfk*s
wc*rrk*rtsriliksfnsishlcfsifnsfilsitl*kcys*i*sif*l*inniscsrr
fl*ffklv**KSMVSIRTYCRWYNLPRLMATASLVHWSLNSLNIT---

---sihctlcqtkrw*scdfrwfp*slpldwskycrrrsyyvlvglwlsiicngqsyrig
***hleq*syrssw*sicih*rrclhtnesigcrlcfsyfwwfnwlqf**yq*ipldg*n
ww*l*sky*rtrlshqwsi*nr*rcltnnvkfshvqt*llsfl*ssh*lsktnrfrsckk
c*nw**kfrfnlfrrsfhicswlvrvykvkdxxnr

Frame B:
f*sinivciciiivyisv*yisifkqinnkkkmkrseksstsvvsnnkqqdvniissnev
gvkeenkghqefllkvlilsviyvlafstrlfsvlryesvihefdpyfnyrstiylvqeg
fynflnwfderawyplgrivggtiypg*wqqqv*figh*ih*ilq---

---PSIVLSAKQNDGSRVIFDGFREAYRWIGQNTADDARIMSWWDYGYQLSAMANRTVLV
DNNTWNNSHIAQVGKAFASTEEDAYIQMKALDVDYVLVIFGGLTGYSSDDINKFLWMVRI
GGSCDPNIKEQDYLTNGQYRIDKGASPTMLNSLMYKLSYYRFSEVHTDYQRPTGFDRVRN
VEIGNKNFDLTYLEEAFTSVHG*lefiklkixiie

Frame C:
fsqsilcvyv**lcilvyniylflnrstirkk*kdqknqvhlllvitnnkm*issvqmkl
vlkkkikdiknsy*kf*fyqsfmf*hfqlvysqyyvmkvlfmnlihiliidqqyilfkkv
fiif*iglmkehgih*dvl*vvqftqvngnskfsslviefieyys---

---hplyslpnktmvvv*fsmvsvkptvglvkilpttlvlclggimvinylqwpivpywl
iitlgtivislklvkhlhplkkmltyk*khwmsimf*lflvv*lvtvlmisinsfgwlel
vvvviqilknkiispmvnie*ikvphqqc*ilsctnlvtivslkftltikdqqvsiv*em
lklvikisi*li*kklshlfmvs*sl*s*rfx**k

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD153 (VHD153Q) /CSM/VH/VHD1-C/VHD153Q.Seq.d/                       2030   0.0
AHQ270 (AHQ270Q) /CSM/AH/AHQ2-C/AHQ270Q.Seq.d/                       1243   0.0
VHK535 (VHK535Q) /CSM/VH/VHK5-B/VHK535Q.Seq.d/                       1241   0.0
VHK440 (VHK440Q) /CSM/VH/VHK4-B/VHK440Q.Seq.d/                       1233   0.0
VHK346 (VHK346Q) /CSM/VH/VHK3-B/VHK346Q.Seq.d/                       1233   0.0
VHJ707 (VHJ707Q) /CSM/VH/VHJ7-A/VHJ707Q.Seq.d/                       1233   0.0
VHD769 (VHD769Q) /CSM/VH/VHD7-C/VHD769Q.Seq.d/                       1233   0.0
VHD508 (VHD508Q) /CSM/VH/VHD5-A/VHD508Q.Seq.d/                       1233   0.0
VHC490 (VHC490Q) /CSM/VH/VHC4-D/VHC490Q.Seq.d/                       1233   0.0
VHC240 (VHC240Q) /CSM/VH/VHC2-B/VHC240Q.Seq.d/                       1233   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CS494261|CS494261.1 Sequence 103 from Patent WO2007006911.         94   2e-34  6
AX799082|AX799082.1 Sequence 1 from Patent WO03054008.             94   2e-34  6
AY074880|AY074880.1 Homo sapiens source of immunodominant MHC-associated peptides (SIMP) mRNA, complete cds.    94   2e-34  6
BU094108|BU094108.1 rf50d02.y2 Meloidogyne hapla J2 pAMP1 v1 Meloidogyne hapla cDNA 5' similar to SW:STT3_MOUSE P46978 OLIGOSACCHARYL TRANSFERASE STT3 SUBUNIT HOMOLOG ;, mRNA sequence.    80   7e-24  3
AC104643|AC104643.2 Homo sapiens chromosome 3 clone RP11-356I4, complete sequence.    86   1e-23  9
CZ536438|CZ536438.1 SRAA-aad03c05.b1 Strongyloides ratti whole genome shotgun library (SRAAGSS 004) Strongyloides ratti genomic, genomic survey sequence.    54   2e-23  5
AM648058|AM648058.1 Entamoeba moshkovskii FIC GSS, clone mosh055c04.p1k.    90   1e-22  2
BH161052|BH161052.1 ENTQZ29TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    78   6e-22  2
AZ546884|AZ546884.1 ENTEI54TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    78   7e-22  2
CV566713|CV566713.1 taj98f09.y1 Hydra EST UCI 6 Hydra magnipapillata cDNA 5' similar to SW:STT3_MOUSE P46978 OLIGOSACCHARYL TRANSFERASE STT3 SUBUNIT HOMOLOG ;, mRNA sequence.    64   2e-21  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT064584_1(BT064584|pid:none) Zea mays full-length cDNA clone ZM...   296   7e-79
AC015446_5(AC015446|pid:none) Arabidopsis thaliana chromosome I ...   293   1e-78
AK229582_1(AK229582|pid:none) Arabidopsis thaliana mRNA for hypo...   293   1e-78
L47337_1(L47337|pid:none) Homo sapiens transmembrane protein (TM...   295   3e-78
AK295892_1(AK295892|pid:none) Homo sapiens cDNA FLJ58575 complet...   294   5e-78
CQ834708_1(CQ834708|pid:none) Sequence 579 from Patent WO2004058...   294   5e-78
AY891653_1(AY891653|pid:none) Synthetic construct Homo sapiens c...   294   5e-78
CQ834710_1(CQ834710|pid:none) Sequence 581 from Patent WO2004058...   294   5e-78
(P46977) RecName: Full=Dolichyl-diphosphooligosaccharide--protei...   294   5e-78
BC048348_1(BC048348|pid:none) Homo sapiens STT3, subunit of the ...   294   5e-78

 psg: 0.68  gvh: 0.44  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.52  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 76.0 %: mitochondrial
	 16.0 %: nuclear
	  8.0 %: cytoplasmic

>> prediction for VHD153 is mit

>VHD153F.Seq
ATTTTAGTCAATCAATATTGTGTGTATATGTATAATAATTGTGTATATTAGTGTATAATA
TATATCTATTTTTAAACAGATCAACAATAAGAAAAAAATGAAAAGATCAGAAAAATCAAG
TACATCTGTTGTTAGTAATAACAAACAACAAGATGTAAATATCATCAGTTCAAATGAAGT
TGGTGTTAAAGAAGAAAATAAAGGACATCAAGAATTCTTATTAAAAGTTTTAATTCTATC
AGTCATTTATGTTTTAGCATTTTCAACTCGTTTATTCTCAGTATTACGTTATGAAAGTGT
TATTCATGAATTTGATCCATATTTTAATTATAGATCAACAATATATCTTGTTCAAGAAGG
TTTTTATAATTTTTTAAATTGGTTTGATGAAAGAGCATGGTATCCATTAGGACGTATTGT
AGGTGGTACAATTTACCCAGGTTAATGGCAACAGCAAGTTTAGTTCATTGGTCATTGAAT
TCATTGAATATTACAGTNNNNNNNNNN

>VHD153Z.Seq
NNNNNNNNNNTCCATCCATTGTACTCTCTGCCAAACAAAACGATGGTAGTCGTGTGATTT
TCGATGGTTTCCGTGAAGCCTACCGTTGGATTGGTCAAAATACTGCCGACGACGCTCGTA
TTATGTCTTGGTGGGATTATGGTTATCAATTATCTGCAATGGCCAATCGTACCGTATTGG
TTGATAATAACACTTGGAACAATAGTCATATCGCTCAAGTTGGTAAAGCATTTGCATCCA
CTGAAGAAGATGCTTACATACAAATGAAAGCATTGGATGTCGATTATGTTTTAGTTATTT
TTGGTGGTTTAACTGGTTACAGTTCTGATGATATCAATAAATTCCTTTGGATGGTTAGAA
TTGGTGGTAGTTGTGATCCAAATATTAAAGAACAAGATTATCTCACCAATGGTCAATATA
GAATAGATAAAGGTGCCTCACCAACAATGTTAAATTCTCTCATGTACAAACTTAGTTACT
ATCGTTTCTCTGAAGTTCACACTGACTATCAAAGACCAACAGGTTTCGATCGTGTAAGAA
ATGTTGAAATTGGTAATAAAAATTTCGATTTAACTTATTTAGAAGAAGCTTTCACATCTG
TTCATGGTTAGTTAGAGTTTATAAAGTTAAAGATTTNNATAATAGAAG

>VHD153P.Seq
ATTTTAGTCAATCAATATTGTGTGTATATGTATAATAATTGTGTATATTAGTGTATAATA
TATATCTATTTTTAAACAGATCAACAATAAGAAAAAAATGAAAAGATCAGAAAAATCAAG
TACATCTGTTGTTAGTAATAACAAACAACAAGATGTAAATATCATCAGTTCAAATGAAGT
TGGTGTTAAAGAAGAAAATAAAGGACATCAAGAATTCTTATTAAAAGTTTTAATTCTATC
AGTCATTTATGTTTTAGCATTTTCAACTCGTTTATTCTCAGTATTACGTTATGAAAGTGT
TATTCATGAATTTGATCCATATTTTAATTATAGATCAACAATATATCTTGTTCAAGAAGG
TTTTTATAATTTTTTAAATTGGTTTGATGAAAGAGCATGGTATCCATTAGGACGTATTGT
AGGTGGTACAATTTACCCAGGTTAATGGCAACAGCAAGTTTAGTTCATTGGTCATTGAAT
TCATTGAATATTACAGT----------TCCATCCATTGTACTCTCTGCCAAACAAAACGA
TGGTAGTCGTGTGATTTTCGATGGTTTCCGTGAAGCCTACCGTTGGATTGGTCAAAATAC
TGCCGACGACGCTCGTATTATGTCTTGGTGGGATTATGGTTATCAATTATCTGCAATGGC
CAATCGTACCGTATTGGTTGATAATAACACTTGGAACAATAGTCATATCGCTCAAGTTGG
TAAAGCATTTGCATCCACTGAAGAAGATGCTTACATACAAATGAAAGCATTGGATGTCGA
TTATGTTTTAGTTATTTTTGGTGGTTTAACTGGTTACAGTTCTGATGATATCAATAAATT
CCTTTGGATGGTTAGAATTGGTGGTAGTTGTGATCCAAATATTAAAGAACAAGATTATCT
CACCAATGGTCAATATAGAATAGATAAAGGTGCCTCACCAACAATGTTAAATTCTCTCAT
GTACAAACTTAGTTACTATCGTTTCTCTGAAGTTCACACTGACTATCAAAGACCAACAGG
TTTCGATCGTGTAAGAAATGTTGAAATTGGTAATAAAAATTTCGATTTAACTTATTTAGA
AGAAGCTTTCACATCTGTTCATGGTTAGTTAGAGTTTATAAAGTTAAAGATTTNNATAAT
AGAAG