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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHD120
Library VH
(Link to library)
Clone ID VHD120
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12400-1|Contig-U16431-1
Original site URL
Representative seq. ID VHD120P
(Link to Original site)
Representative DNA sequence 
>VHD120 (VHD120Q) /CSM/VH/VHD1-A/VHD120Q.Seq.d/
AATAAAATGATAAGATCATCAATAAAAAATAAAATAACAACAACAAAAAGTTTATCATGT
ATTTCATTAATAAATAGACAATATGGTACATCAACAAAAGAAGTACGTGAAACATTTTTA
AATTATTTTGAAAAAAATGGTCATAAAAGATTACCAAGTGGTAGTTTATTACCATATAAT
GATAATTCATTATTATTTACAAATGCAGGTATGGTACAATTTAAAAATCAATTTACAGGT
AATGAAGAATCAAAATATAAAAAAGTTACAACATCACAAAAATGTGTTAGAGCAGGTGGT
AAACATAATGATTTAGATAATGTTGGATATACAGCAAGACATCATACATTTTTTGAAATG
TTGGGTAATTTTTCATTTGGAGGATATAATCATTTTAAAAGAGATTCAATTCAACATGCA
TGGGATTTATTGACAAAAGAATATGGTTTACCAAAAGAGAGATTAGCAATTTCAGTTTTA
GAGGGTGATGAAGAATCAGCAGAAATTTGGAGAAATCAAATTGGATTACCAAATGATAAG
ATTATGTATAAAGGTAGAGAGGATAATTTTTGGTCAATGGGTGATGGTCCTGGACCXXXX
XXXXXXATGGTACTTCAGGTGGTCAAGTTGGTGATGTTGGTGAATTAATCAATGTTTCAA
GTGGTAAAAATGTTTATCGTGTAATTAATACAATTAAACCATATGAAGGTGGTTTAGTTT
TAGTAGTTGAATGGGATCCTTCACAACAATTGGCTTCACAGGTTTATCAAGATTTAAAAC
AAGATTCATTATTGAATTGTAGAGTGGATCGTTCAATTAGAAATCAAGTTGCTGTTCATC
ATAGTGCTACTCATTTGCTACATGCAGCATTAAGAAATGTAATTGGCAAAAGTGTAGTTC
AAGCTGGTTCATTGGTTGGTAGTGAATCATTACGTTTCGATTTCACACATGGTCAAAAAT
TAACACCAAATCAAATTGAACAAATTGAACAATGGGTAAATGATGCAATTGCCAAAGATA
TAGCTTTGAATACCGATGAAATTCCATATGAACAAGCATCTAAAAATAGTGATACCCTTC
AATTATTTAGTGAAAAGTATAGTGAATTGGTACGTGTTGTTAGTATACCTGGATTCTCTA
AGGAGCTCTGTGGTGGTACACATGTTGAACGTTCATCTTCAATTCATCAATTTAAAATTA
TAAGTGAAAGTAGTGTAGCAGCAGGAACTCGTAGAATTGAAGCAGTGGCAGGTTTAGCAG
CAACAAACTTTTTCAAAAATCATTATCAATTAGTTCATCAATTATCAAATAGTATAAATT
CGCCAATAGTTAATTTCCAACAATCATTG
sequence update 2002.10.25
Translated Amino Acid sequence 
NKMIRSSIKNKITTTKSLSCISLINRQYGTSTKEVRETFLNYFEKNGHKRLPSGSLLPYN
DNSLLFTNAGMVQFKNQFTGNEESKYKKVTTSQKCVRAGGKHNDLDNVGYTARHHTFFEM
LGNFSFGGYNHFKRDSIQHAWDLLTKEYGLPKERLAISVLEGDEESAEIWRNQIGLPNDK
IMYKGREDNFWSMGDGPG---

---GTSGGQVGDVGELINVSSGKNVYRVINTIKPYEGGLVLVVEWDPSQQLASQVYQDLK
QDSLLNCRVDRSIRNQVAVHHSATHLLHAALRNVIGKSVVQAGSLVGSESLRFDFTHGQK
LTPNQIEQIEQWVNDAIAKDIALNTDEIPYEQASKNSDTLQLFSEKYSELVRVVSIPGFS
KELCGGTHVERSSSIHQFKIISESSVAAGTRRIEAVAGLAATNFFKNHYQLVHQLSNSIN
SPIVNFQQSL
Translated Amino Acid sequence (All Frames) 
Frame A:
NKMIRSSIKNKITTTKSLSCISLINRQYGTSTKEVRETFLNYFEKNGHKRLPSGSLLPYN
DNSLLFTNAGMVQFKNQFTGNEESKYKKVTTSQKCVRAGGKHNDLDNVGYTARHHTFFEM
LGNFSFGGYNHFKRDSIQHAWDLLTKEYGLPKERLAISVLEGDEESAEIWRNQIGLPNDK
IMYKGREDNFWSMGDGPG---

---mvlqvvklvmlvn*smfqvvkmfiv*liqlnhmkvv*f**lngilhnnwlhrfiki*
nkihy*ivewivqleikllfiivllicymqh*em*lakv*fklvhwlvvnhyvsishmvk
n*hqiklnklnng*mmqlpki*l*ipmkfhmnkhlkivipfnylvksivnwyvllvylds
lrssvvvhmlnvhlqfinlkl*vkvv*qqelvelkqwqv*qqqtfskiiin*finyqiv*
irq*lisnnh

Frame B:
ik**dhq*kik*qqqkvyhvfh**idnmvhqqkkyvkhf*iilkkmvikdyqvvvyyhim
iihyylqmqvwynlkinlqvmknqnikklqhhknvleqvvnimi*imldiqqdiihflkc
wvifhlediiilkeiqfnmhgiy*qknmvyqkrd*qfqf*rvmknqqkfgeikldyqmir
lcikveriifgqwvmvld---

---wyfrwssw*cw*inqcfkw*kclscn*yn*ti*rwfsfss*mgsfttigftglsrfk
trfiiel*sgsfn*kssccss*cysfatcsikkcnwqkcssswfigw**iitfrfhtwsk
intksn*tn*tmgk*cncqrysfeyr*nsi*tsi*k**ypsii**kv**igtcc*ytwil
*galwwytc*tfifnssi*nyk*k*cssrns*n*ssgrfssnklfqkslsisssiik*yk
fans*fptii

Frame C:
*ndkiinkk*nnnnkkfimyfink*tiwyinkrst*nifklf*kkws*kitkw*fiti**
*fiiiykcrygti*ksiyr**riki*ksynitkmc*srw*t**fr*cwiysktsyif*nv
g*ffiwri*sf*krfnstcmgfidkriwftkreisnfsfrg**risrnleksnwitk**d
yv*r*rg*flvng*wswt---

---GTSGGQVGDVGELINVSSGKNVYRVINTIKPYEGGLVLVVEWDPSQQLASQVYQDLK
QDSLLNCRVDRSIRNQVAVHHSATHLLHAALRNVIGKSVVQAGSLVGSESLRFDFTHGQK
LTPNQIEQIEQWVNDAIAKDIALNTDEIPYEQASKNSDTLQLFSEKYSELVRVVSIPGFS
KELCGGTHVERSSSIHQFKIISESSVAAGTRRIEAVAGLAATNFFKNHYQLVHQLSNSIN
SPIVNFQQSL
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD120 (VHD120Q) /CSM/VH/VHD1-A/VHD120Q.Seq.d/                       2476   0.0
VHL882 (VHL882Q) /CSM/VH/VHL8-D/VHL882Q.Seq.d/                       1441   0.0
VHP273 (VHP273Q) /CSM/VH/VHP2-D/VHP273Q.Seq.d/                       1043   0.0
VHG721 (VHG721Q) /CSM/VH/VHG7-A/VHG721Q.Seq.d/                       1043   0.0
SHK547 (SHK547Q) /CSM/SH/SHK5-B/SHK547Q.Seq.d/                       1043   0.0
AHO389 (AHO389Q) /CSM/AH/AHO3-D/AHO389Q.Seq.d/                       1043   0.0
VHF210 (VHF210Q) /CSM/VH/VHF2-A/VHF210Q.Seq.d/                       1029   0.0
VHB514 (VHB514Q) /CSM/VH/VHB5-A/VHB514Q.Seq.d/                       1023   0.0
CHQ319 (CHQ319Q) /CSM/CH/CHQ3-A/CHQ319Q.Seq.d/                        743   0.0
AHO189 (AHO189Q) /CSM/AH/AHO1-D/AHO189Q.Seq.d/                        573   e-162
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.  1471   0.0    1
EJ874219|EJ874219.1 1093018360709 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061002807102 3', genomic survey sequence.   161   6e-38  3
EJ798763|EJ798763.1 1093017414825 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061002807102 5', genomic survey sequence.   161   1e-36  2
ER312489|ER312489.1 1092343803475 Global-Ocean-Sampling_GS-34-01-01-1P5-1P8KB marine metagenome genomic clone 1061001480902 3', genomic survey sequence.   137   1e-35  3
ER580532|ER580532.1 1093015835772 Global-Ocean-Sampling_GS-36-01-01-2P2KB marine metagenome genomic clone 1061002567471 3', genomic survey sequence.   145   1e-33  2
EK387716|EK387716.1 1095469489584 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005887797 3', genomic survey sequence.   135   3e-33  3
ER531046|ER531046.1 1093015751259 Global-Ocean-Sampling_GS-35-01-01-1P5KB marine metagenome genomic clone 1061002525356 5', genomic survey sequence.   137   3e-33  3
EK140713|EK140713.1 1095454102566 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005675929 3', genomic survey sequence.   115   4e-33  3
EK189963|EK189963.1 1095460015365 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005692293 3', genomic survey sequence.   135   4e-33  2
EK576736|EK576736.1 1095521353329 Global-Ocean-Sampling_GS-32-01-01-1P3-1P6KB marine metagenome genomic clone 1061005969790 3', genomic survey sequence.   155   4e-33  1
dna update 2007. 5. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115598_31(AC115598|pid:none) Dictyostelium discoideum chromoso...   450   e-125
CP001196_1415(CP001196|pid:none) Oligotropha carboxidovorans OM5...   225   2e-57
(A9IVY8) RecName: Full=Alanyl-tRNA synthetase;          EC=6.1.1...   224   4e-57
(A6X0E9) RecName: Full=Alanyl-tRNA synthetase;          EC=6.1.1...   224   5e-57
(A5VQX4) RecName: Full=Alanyl-tRNA synthetase;          EC=6.1.1...   224   7e-57
(Q2G8V0) RecName: Full=Alanyl-tRNA synthetase;          EC=6.1.1...   223   9e-57
(Q2RQI0) RecName: Full=Alanyl-tRNA synthetase;          EC=6.1.1...   222   2e-56
CP000394_1369(CP000394|pid:none) Granulibacter bethesdensis CGDN...   222   3e-56
CP000613_878(CP000613|pid:none) Rhodospirillum centenum SW, comp...   221   4e-56
AE008692_845(AE008692|pid:none) Zymomonas mobilis subsp. mobilis...   221   4e-56
protein update 2009. 6.28
PSORT 

 psg: 0.77  gvh: 0.33  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.54  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 76.0 %: mitochondrial
	 12.0 %: cytoplasmic
	  8.0 %: nuclear
	  4.0 %: cytoskeletal

>> prediction for VHD120 is mit
5' end seq. ID VHD120F
5' end seq. 
>VHD120F.Seq
AATAAAATGATAAGATCATCAATAAAAAATAAAATAACAACAACAAAAAGTTTATCATGT
ATTTCATTAATAAATAGACAATATGGTACATCAACAAAAGAAGTACGTGAAACATTTTTA
AATTATTTTGAAAAAAATGGTCATAAAAGATTACCAAGTGGTAGTTTATTACCATATAAT
GATAATTCATTATTATTTACAAATGCAGGTATGGTACAATTTAAAAATCAATTTACAGGT
AATGAAGAATCAAAATATAAAAAAGTTACAACATCACAAAAATGTGTTAGAGCAGGTGGT
AAACATAATGATTTAGATAATGTTGGATATACAGCAAGACATCATACATTTTTTGAAATG
TTGGGTAATTTTTCATTTGGAGGATATAATCATTTTAAAAGAGATTCAATTCAACATGCA
TGGGATTTATTGACAAAAGAATATGGTTTACCAAAAGAGAGATTAGCAATTTCAGTTTTA
GAGGGTGATGAAGAATCAGCAGAAATTTGGAGAAATCAAATTGGATTACCAAATGATAAG
ATTATGTATAAAGGTAGAGAGGATAATTTTTGGTCAATGGGTGATGGTCCTGGACCNNNN
NNNNNN
Length of 5' end seq. 606
3' end seq. ID VHD120Z
3' end seq. 
>VHD120Z.Seq
NNNNNNNNNNATGGTACTTCAGGTGGTCAAGTTGGTGATGTTGGTGAATTAATCAATGTT
TCAAGTGGTAAAAATGTTTATCGTGTAATTAATACAATTAAACCATATGAAGGTGGTTTA
GTTTTAGTAGTTGAATGGGATCCTTCACAACAATTGGCTTCACAGGTTTATCAAGATTTA
AAACAAGATTCATTATTGAATTGTAGAGTGGATCGTTCAATTAGAAATCAAGTTGCTGTT
CATCATAGTGCTACTCATTTGCTACATGCAGCATTAAGAAATGTAATTGGCAAAAGTGTA
GTTCAAGCTGGTTCATTGGTTGGTAGTGAATCATTACGTTTCGATTTCACACATGGTCAA
AAATTAACACCAAATCAAATTGAACAAATTGAACAATGGGTAAATGATGCAATTGCCAAA
GATATAGCTTTGAATACCGATGAAATTCCATATGAACAAGCATCTAAAAATAGTGATACC
CTTCAATTATTTAGTGAAAAGTATAGTGAATTGGTACGTGTTGTTAGTATACCTGGATTC
TCTAAGGAGCTCTGTGGTGGTACACATGTTGAACGTTCATCTTCAATTCATCAATTTAAA
ATTATAAGTGAAAGTAGTGTAGCAGCAGGAACTCGTAGAATTGAAGCAGTGGCAGGTTTA
GCAGCAACAAACTTTTTCAAAAATCATTATCAATTAGTTCATCAATTATCAAATAGTATA
AATTCGCCAATAGTTAATTTCCAACAATCATTG
Length of 3' end seq. 753
Connected seq. ID VHD120P
Connected seq. 
>VHD120P.Seq
AATAAAATGATAAGATCATCAATAAAAAATAAAATAACAACAACAAAAAGTTTATCATGT
ATTTCATTAATAAATAGACAATATGGTACATCAACAAAAGAAGTACGTGAAACATTTTTA
AATTATTTTGAAAAAAATGGTCATAAAAGATTACCAAGTGGTAGTTTATTACCATATAAT
GATAATTCATTATTATTTACAAATGCAGGTATGGTACAATTTAAAAATCAATTTACAGGT
AATGAAGAATCAAAATATAAAAAAGTTACAACATCACAAAAATGTGTTAGAGCAGGTGGT
AAACATAATGATTTAGATAATGTTGGATATACAGCAAGACATCATACATTTTTTGAAATG
TTGGGTAATTTTTCATTTGGAGGATATAATCATTTTAAAAGAGATTCAATTCAACATGCA
TGGGATTTATTGACAAAAGAATATGGTTTACCAAAAGAGAGATTAGCAATTTCAGTTTTA
GAGGGTGATGAAGAATCAGCAGAAATTTGGAGAAATCAAATTGGATTACCAAATGATAAG
ATTATGTATAAAGGTAGAGAGGATAATTTTTGGTCAATGGGTGATGGTCCTGGACC----
------ATGGTACTTCAGGTGGTCAAGTTGGTGATGTTGGTGAATTAATCAATGTTTCAA
GTGGTAAAAATGTTTATCGTGTAATTAATACAATTAAACCATATGAAGGTGGTTTAGTTT
TAGTAGTTGAATGGGATCCTTCACAACAATTGGCTTCACAGGTTTATCAAGATTTAAAAC
AAGATTCATTATTGAATTGTAGAGTGGATCGTTCAATTAGAAATCAAGTTGCTGTTCATC
ATAGTGCTACTCATTTGCTACATGCAGCATTAAGAAATGTAATTGGCAAAAGTGTAGTTC
AAGCTGGTTCATTGGTTGGTAGTGAATCATTACGTTTCGATTTCACACATGGTCAAAAAT
TAACACCAAATCAAATTGAACAAATTGAACAATGGGTAAATGATGCAATTGCCAAAGATA
TAGCTTTGAATACCGATGAAATTCCATATGAACAAGCATCTAAAAATAGTGATACCCTTC
AATTATTTAGTGAAAAGTATAGTGAATTGGTACGTGTTGTTAGTATACCTGGATTCTCTA
AGGAGCTCTGTGGTGGTACACATGTTGAACGTTCATCTTCAATTCATCAATTTAAAATTA
TAAGTGAAAGTAGTGTAGCAGCAGGAACTCGTAGAATTGAAGCAGTGGCAGGTTTAGCAG
CAACAAACTTTTTCAAAAATCATTATCAATTAGTTCATCAATTATCAAATAGTATAAATT
CGCCAATAGTTAATTTCCAACAATCATTG
Length of connected seq. 1339
Full length Seq ID -
Full length Seq. -
Length of full length seq. -