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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHC801
Library VH
(Link to library)
Clone ID VHC801
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14941-1
Original site URL
Representative seq. ID VHC801P
(Link to Original site)
Representative DNA sequence 
>VHC801 (VHC801Q) /CSM/VH/VHC8-A/VHC801Q.Seq.d/
AACAGAAGAAGATCAAAAATTATTCCTTTCAATTGGATTAGATGAAAAGAGAGCAAAAGA
TACTTTAAAAAATAAAGATCTTACTGCATTATTAAAAACCATTATTGAAGAAGCATCCGT
TTCAAAAGGTTGTGATAAATCAATTGGTAACCTTTTATATAGTTTAGCAACTTGTCAACA
AGTAAACTCACCAATTCGTTCAAACGTTTCAAAAGCAATTGGTGAAGAAAAAATTAAAAC
CACCATTCAATTCCAAGCTGCAACTCAATACTTAAAAGATAACAAAGAATTCAATCAAGA
TACATTTAACGATTTTTGTGGTGTTGGTGTTGTCATCACACCAGAACAAATTCAAAAAGC
TATTGATGATTTATTCACTAAAAAAGCTACTGAAATTTCTGAAAAAAAATGGCATATACC
AATTGGTGATTTATTAAATCCATTAAAAGAATCATTAAAATGGGCAGATTTAAAAGAAGT
TAAAATAATGTTAGATAAAAAATTAGAGACAACATTAGGACCAAAAGTTGTTGAAGCTAA
AGAAAAGAAAGTAGCAGCAACAACCACTGCACCAGTTAAXXXXXXXXXXCTATGTTACCA
GTACTCGGTGACAATTTCACCATCACTCAAAGTTATCGTGCTGAAAAGGCTCGTACTCGT
CGTCATTTAACCGAGTTTTTACATTTGGAAGCTGAATGTCCATTCATCACCTACGAGGAA
CTTCAAGATCGTATCGAATTCTTGGTTGTCGATGTTTGTGAAAAACTCTTCAAAATGGAC
CCAGAATTGATCCTCTCTGTCAATCCAGACTTTAAAGTACCAAAGAGACCATTCATGAGA
ATGAACTATTCCGATGCTATCGAATATTGTAAAAAGAATGGTATCCAAAAGAAACTTGAA
GATGGTACTGAAGTCGATTTCGAATTTGGTGATGATATTCCAGAGGCTCAAGAAAGAAGA
ATGAATGATCAAATCGGTGAACCAATCTTCCTCTGTAGATTCCCTGCTGAAATGAAAGCT
TTCTACATGGCTAGATGTCCAGAAGATAGAACTCTCACTGAATCTCTTGATCTCTTAATG
CCAGGTGTTGGTGAAATCGTTGGTGGTTCTATGAGAATTTCAGATTTCAATGAATTGGTT
GCAGCTTATAAAAAAGCTGGTCTTGATACTGATGAATATTATTGGTTCACTGATCAACGT
AAATATGGTACTACTCAACATGGTGGTTATGGTTTAGGTGTTGAACGTTTCCTTACTTGG
ATGCTCAAAGAAGAACATATTCGTAATGTTTGTGTTTACCAACGTACAAAAGATAGAATT
ACCCCATAAAATATTTTTTTGATTTTAATTTCTCAATTCTAAAATCATTTTGATCAAATA
AAGTGTTTCT
sequence update 2002.10.25
Translated Amino Acid sequence 
TEEDQKLFLSIGLDEKRAKDTLKNKDLTALLKTIIEEASVSKGCDKSIGNLLYSLATCQQ
VNSPIRSNVSKAIGEEKIKTTIQFQAATQYLKDNKEFNQDTFNDFCGVGVVITPEQIQKA
IDDLFTKKATEISEKKWHIPIGDLLNPLKESLKWADLKEVKIMLDKKLETTLGPKVVEAK
EKKVAATTTAPV---

---MLPVLGDNFTITQSYRAEKARTRRHLTEFLHLEAECPFITYEELQDRIEFLVVDVCE
KLFKMDPELILSVNPDFKVPKRPFMRMNYSDAIEYCKKNGIQKKLEDGTEVDFEFGDDIP
EAQERRMNDQIGEPIFLCRFPAEMKAFYMARCPEDRTLTESLDLLMPGVGEIVGGSMRIS
DFNELVAAYKKAGLDTDEYYWFTDQRKYGTTQHGGYGLGVERFLTWMLKEEHIRNVCVYQ
RTKDRITP*niflilisqf*nhfdqikcf
Translated Amino Acid sequence (All Frames) 
Frame A:
nrrrskiipfnwir*keskryfkk*rsyciiknhy*rsirfkrl**inw*pfi*fsnlst
skltnsfkrfksnw*rkn*nhhsipscnsilkr*qriqsryi*rflwcwcchhtrtnsks
y**fih*ksy*nf*kkmaytnw*fiksikriikmgrfkrs*nnvr*kirdnirtksc*s*
rkesssnnhcts*---

---lcyqysvtispslkvivlkrlvlvvi*psfyiwklnvhssptrnfkivsnswlsmfv
knsskwtqn*sslsiqtlkyqrdhs*e*tipmlsnivkrmvskrnlkmvlksisnlvmif
qrlkkee*miksvnqsssvdsllk*klstwldvqkielslnllis*cqvlvkslvvl*ef
qismnwlqlikklvlilmniigslinvnmvllnmvvmv*vlnvsllgcskknifvmfvft
nvqkielphkiff*f*flnskiilik*svs

Frame B:
TEEDQKLFLSIGLDEKRAKDTLKNKDLTALLKTIIEEASVSKGCDKSIGNLLYSLATCQQ
VNSPIRSNVSKAIGEEKIKTTIQFQAATQYLKDNKEFNQDTFNDFCGVGVVITPEQIQKA
IDDLFTKKATEISEKKWHIPIGDLLNPLKESLKWADLKEVKIMLDKKLETTLGPKVVEAK
EKKVAATTTAPV---

---yvtstr*qfhhhsklsc*kgsysssfnrvftfgs*msihhlrgtsrsyrilgcrcl*
ktlqngpridplcqsrl*stketihenelfrcyril*kewypket*rwy*srfriw**ys
rgsrkkne*snr*tnlpl*ipc*nesflhg*msrr*nsh*is*slnarcw*nrwwfyenf
rfq*igcsl*ksws*y**illvh*st*iwyystwwlwfrc*tfpyldaqrrtys*clclp
tykr*nypikyffdfnfsilksf*snkvf

Frame C:
qkkiknysfqld*mkreqkil*kikillhy*kpllkkhpfqkvvinqlvtfyiv*qlvnk
*thqfvqtfqkqlvkkklkppfnsklqlnt*kitknsikihltifvvlvlsshqnkfkkl
lmiyslkkllkflkkngiyqlviy*ih*knh*ngqi*kklk*c*ikn*rqh*dqkllklk
krk*qqqplhql---

---MLPVLGDNFTITQSYRAEKARTRRHLTEFLHLEAECPFITYEELQDRIEFLVVDVCE
KLFKMDPELILSVNPDFKVPKRPFMRMNYSDAIEYCKKNGIQKKLEDGTEVDFEFGDDIP
EAQERRMNDQIGEPIFLCRFPAEMKAFYMARCPEDRTLTESLDLLMPGVGEIVGGSMRIS
DFNELVAAYKKAGLDTDEYYWFTDQRKYGTTQHGGYGLGVERFLTWMLKEEHIRNVCVYQ
RTKDRITP*niflilisqf*nhfdqikcf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC801 (VHC801Q) /CSM/VH/VHC8-A/VHC801Q.Seq.d/                       2607   0.0
SFE837 (SFE837Q) /CSM/SF/SFE8-B/SFE837Q.Seq.d/                       1499   0.0
SLE204 (SLE204Q) /CSM/SL/SLE2-A/SLE204Q.Seq.d/                       1479   0.0
VHC634 (VHC634Q) /CSM/VH/VHC6-B/VHC634Q.Seq.d/                       1457   0.0
VHB335 (VHB335Q) /CSM/VH/VHB3-B/VHB335Q.Seq.d/                       1441   0.0
VHI167 (VHI167Q) /CSM/VH/VHI1-C/VHI167Q.Seq.d/                       1433   0.0
SFK826 (SFK826Q) /CSM/SF/SFK8-B/SFK826Q.Seq.d/                       1425   0.0
SFF879 (SFF879Q) /CSM/SF/SFF8-D/SFF879Q.Seq.d/                       1411   0.0
VHM212 (VHM212Q) /CSM/VH/VHM2-A/VHM212Q.Seq.d/                       1372   0.0
CHD536 (CHD536Q) /CSM/CH/CHD5-B/CHD536Q.Seq.d/                       1340   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.  1281   0.0    1
EE004071|EE004071.1 ROE00007211 Rhizopus oryzae Home made (1-5 kb) Glucose Rhizopus oryzae cDNA, mRNA sequence.    98   8e-16  1
AM643549|AM643549.1 Entamoeba dispar GSS, clone dispar85b10.p1k.    84   2e-14  2
ES217666|ES217666.1 MpFVN_ag1_H24 Myzus persicae, line F001, PLRV free, whole female aphid library Myzus persicae cDNA clone MpFVN_ag1_H24, mRNA sequence.    90   2e-13  1
CX081759|CX081759.1 EHAAM58TR E. histolytica Normalized cDNA library Entamoeba histolytica cDNA, mRNA sequence.    80   5e-13  2
CX089960|CX089960.1 EHAE646TR E. histolytica Normalized cDNA library Entamoeba histolytica cDNA, mRNA sequence.    80   6e-13  2
AZ692581|AZ692581.1 ENTGY45TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   2e-10  1
CN761262|CN761262.1 ID0AAA2DD07RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA2DD07 5', mRNA sequence.    64   5e-06  2
CN756331|CN756331.1 ID0AAA18BF03RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA18BF03 5', mRNA sequence.    64   1e-05  1
AR548984|AR548984.1 Sequence 4115 from patent US 6747137.          42   2e-04  3
dna update 2007. 5. 8
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116305_93(AC116305|pid:none) Dictyostelium discoideum chromoso...   506   e-142
(O43776) RecName: Full=Asparaginyl-tRNA synthetase, cytoplasmic;...   313   9e-84
AK297777_1(AK297777|pid:none) Homo sapiens cDNA FLJ52703 complet...   313   9e-84
(Q8BP47) RecName: Full=Asparaginyl-tRNA synthetase, cytoplasmic;...   312   2e-83
AK077699_1(AK077699|pid:none) Mus musculus 8 days embryo whole b...   312   2e-83
AE014134_3150(AE014134|pid:none) Drosophila melanogaster chromos...   312   2e-83
AY387099_1(AY387099|pid:none) Rattus norvegicus LRRGT00113 mRNA,...   311   3e-83
BC099099_1(BC099099|pid:none) Rattus norvegicus asparaginyl-tRNA...   311   3e-83
(Q4R4Z1) RecName: Full=Asparaginyl-tRNA synthetase, cytoplasmic;...   311   3e-83
AK222835_1(AK222835|pid:none) Homo sapiens mRNA for asparaginyl-...   311   3e-83
protein update 2009. 6.28
PSORT 

 psg: 0.75  gvh: 0.55  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for VHC801 is cyt
5' end seq. ID VHC801F
5' end seq. 
>VHC801F.Seq
AACAGAAGAAGATCAAAAATTATTCCTTTCAATTGGATTAGATGAAAAGAGAGCAAAAGA
TACTTTAAAAAATAAAGATCTTACTGCATTATTAAAAACCATTATTGAAGAAGCATCCGT
TTCAAAAGGTTGTGATAAATCAATTGGTAACCTTTTATATAGTTTAGCAACTTGTCAACA
AGTAAACTCACCAATTCGTTCAAACGTTTCAAAAGCAATTGGTGAAGAAAAAATTAAAAC
CACCATTCAATTCCAAGCTGCAACTCAATACTTAAAAGATAACAAAGAATTCAATCAAGA
TACATTTAACGATTTTTGTGGTGTTGGTGTTGTCATCACACCAGAACAAATTCAAAAAGC
TATTGATGATTTATTCACTAAAAAAGCTACTGAAATTTCTGAAAAAAAATGGCATATACC
AATTGGTGATTTATTAAATCCATTAAAAGAATCATTAAAATGGGCAGATTTAAAAGAAGT
TAAAATAATGTTAGATAAAAAATTAGAGACAACATTAGGACCAAAAGTTGTTGAAGCTAA
AGAAAAGAAAGTAGCAGCAACAACCACTGCACCAGTTAANNNNNNNNNN
Length of 5' end seq. 589
3' end seq. ID VHC801Z
3' end seq. 
>VHC801Z.Seq
NNNNNNNNNNCTATGTTACCAGTACTCGGTGACAATTTCACCATCACTCAAAGTTATCGT
GCTGAAAAGGCTCGTACTCGTCGTCATTTAACCGAGTTTTTACATTTGGAAGCTGAATGT
CCATTCATCACCTACGAGGAACTTCAAGATCGTATCGAATTCTTGGTTGTCGATGTTTGT
GAAAAACTCTTCAAAATGGACCCAGAATTGATCCTCTCTGTCAATCCAGACTTTAAAGTA
CCAAAGAGACCATTCATGAGAATGAACTATTCCGATGCTATCGAATATTGTAAAAAGAAT
GGTATCCAAAAGAAACTTGAAGATGGTACTGAAGTCGATTTCGAATTTGGTGATGATATT
CCAGAGGCTCAAGAAAGAAGAATGAATGATCAAATCGGTGAACCAATCTTCCTCTGTAGA
TTCCCTGCTGAAATGAAAGCTTTCTACATGGCTAGATGTCCAGAAGATAGAACTCTCACT
GAATCTCTTGATCTCTTAATGCCAGGTGTTGGTGAAATCGTTGGTGGTTCTATGAGAATT
TCAGATTTCAATGAATTGGTTGCAGCTTATAAAAAAGCTGGTCTTGATACTGATGAATAT
TATTGGTTCACTGATCAACGTAAATATGGTACTACTCAACATGGTGGTTATGGTTTAGGT
GTTGAACGTTTCCTTACTTGGATGCTCAAAGAAGAACATATTCGTAATGTTTGTGTTTAC
CAACGTACAAAAGATAGAATTACCCCATAAAATATTTTTTTGATTTTAATTTCTCAATTC
TAAAATCATTTTGATCAAATAAAGTGTTTCT
Length of 3' end seq. 811
Connected seq. ID VHC801P
Connected seq. 
>VHC801P.Seq
AACAGAAGAAGATCAAAAATTATTCCTTTCAATTGGATTAGATGAAAAGAGAGCAAAAGA
TACTTTAAAAAATAAAGATCTTACTGCATTATTAAAAACCATTATTGAAGAAGCATCCGT
TTCAAAAGGTTGTGATAAATCAATTGGTAACCTTTTATATAGTTTAGCAACTTGTCAACA
AGTAAACTCACCAATTCGTTCAAACGTTTCAAAAGCAATTGGTGAAGAAAAAATTAAAAC
CACCATTCAATTCCAAGCTGCAACTCAATACTTAAAAGATAACAAAGAATTCAATCAAGA
TACATTTAACGATTTTTGTGGTGTTGGTGTTGTCATCACACCAGAACAAATTCAAAAAGC
TATTGATGATTTATTCACTAAAAAAGCTACTGAAATTTCTGAAAAAAAATGGCATATACC
AATTGGTGATTTATTAAATCCATTAAAAGAATCATTAAAATGGGCAGATTTAAAAGAAGT
TAAAATAATGTTAGATAAAAAATTAGAGACAACATTAGGACCAAAAGTTGTTGAAGCTAA
AGAAAAGAAAGTAGCAGCAACAACCACTGCACCAGTTAA----------CTATGTTACCA
GTACTCGGTGACAATTTCACCATCACTCAAAGTTATCGTGCTGAAAAGGCTCGTACTCGT
CGTCATTTAACCGAGTTTTTACATTTGGAAGCTGAATGTCCATTCATCACCTACGAGGAA
CTTCAAGATCGTATCGAATTCTTGGTTGTCGATGTTTGTGAAAAACTCTTCAAAATGGAC
CCAGAATTGATCCTCTCTGTCAATCCAGACTTTAAAGTACCAAAGAGACCATTCATGAGA
ATGAACTATTCCGATGCTATCGAATATTGTAAAAAGAATGGTATCCAAAAGAAACTTGAA
GATGGTACTGAAGTCGATTTCGAATTTGGTGATGATATTCCAGAGGCTCAAGAAAGAAGA
ATGAATGATCAAATCGGTGAACCAATCTTCCTCTGTAGATTCCCTGCTGAAATGAAAGCT
TTCTACATGGCTAGATGTCCAGAAGATAGAACTCTCACTGAATCTCTTGATCTCTTAATG
CCAGGTGTTGGTGAAATCGTTGGTGGTTCTATGAGAATTTCAGATTTCAATGAATTGGTT
GCAGCTTATAAAAAAGCTGGTCTTGATACTGATGAATATTATTGGTTCACTGATCAACGT
AAATATGGTACTACTCAACATGGTGGTTATGGTTTAGGTGTTGAACGTTTCCTTACTTGG
ATGCTCAAAGAAGAACATATTCGTAATGTTTGTGTTTACCAACGTACAAAAGATAGAATT
ACCCCATAAAATATTTTTTTGATTTTAATTTCTCAATTCTAAAATCATTTTGATCAAATA
AAGTGTTTCT
Length of connected seq. 1380
Full length Seq ID -
Full length Seq. -
Length of full length seq. -