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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFN587
Library AF
(Link to library)
Clone ID AFN587
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10734-1
Original site URL
Representative seq. ID AFN587P
(Link to Original site)
Representative DNA sequence 
>AFN587 (AFN587Q) /CSM/AF/AFN5-D/AFN587Q.Seq.d/
TGAATAAATTTTTAGCTTTGTTATTTGTTTTAGCTTTGTTNGCTAATATTGCTTTTTCAT
GNGAAGGNCACCCAGAACATGATCATGGGGATGGGGATCATGANCATGATCATGATGAAT
CATTTGTTAAAATNTTANATTCTGATAATTTCCATAATTCAGTTTNTGAACATGACGTAA
CTNTTGTTNTGTTTTATGCCCCATGGXXXXXXXXXXTTATTTATTCATTGACTCCACTCA
AGATAACACTCAAGTTCTCGAAGACGTAAAGAAAATCGCTACCTCACAAAAAGGTAATGC
CGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTC
TGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTT
CGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGG
TGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGT
TAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTT
GGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAA
ATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTC
AAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGC
TGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGT
TGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGATGA
TAATGTTAATCTAAAAAAGATTCTTCTGCTAAACA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
NKFLALLFVLALXANIAFSXEGHPEHDHGDGDHXHDHDESFVKXLXSDNFHNSVXEHDVT
XVXFYAPW---

---YLFIDSTQDNTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISV
DSVANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFK
KLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEI
RGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTDDNVNLKKILL
LN
Translated Amino Acid sequence (All Frames) 
Frame A:
*inf*lcylf*lcxlillfhxkxtqnmimgmgimxmimmnhllkxxiliisiiqfxnmt*
llxcfmph---

---lfih*lhsr*hsssrrrkenryltkr*crfllgryeeipttsnsygslw*scsshlc
*fscq*sslqfr*krnlliryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfq
kvgfrltkgcfg*ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn
*rlpnhhalqs**qrksnll*rskk*syelc*ihsr*rch*iqitiisn***c*skkdss
ak

Frame B:
e*ifsfvicfsfvx*ycffmxrxprt*swgwgs*x*s**iic*nxxf**fp*fsx*t*rn
xcxvlcpm---

---YLFIDSTQDNTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISV
DSVANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFK
KLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEI
RGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTDDNVNLKKILL
LN

Frame C:
NKFLALLFVLALXANIAFSXEGHPEHDHGDGDHXHDHDESFVKXLXSDNFHNSVXEHDVT
XVXFYAPW---

---iysltplkitlkfskt*rkslphkkvmpfsvgsi*rnshnkqliwvslvklfqpsll
iqlpiklvtismkkkpshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplsk
swf*thqrmfwlnstlhgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqviskl
evtqpscssklmtkkiqslmkvkemii*tllnsfkitlplnsnyhhlklmimli*krffc
*t
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFN587 (AFN587Q) /CSM/AF/AFN5-D/AFN587Q.Seq.d/                       1740   0.0
AFO368 (AFO368Q) /CSM/AF/AFO3-C/AFO368Q.Seq.d/                       1411   0.0
AFL563 (AFL563Q) /CSM/AF/AFL5-C/AFL563Q.Seq.d/                       1411   0.0
AFN855 (AFN855Q) /CSM/AF/AFN8-C/AFN855Q.Seq.d/                       1409   0.0
AFM464 (AFM464Q) /CSM/AF/AFM4-C/AFM464Q.Seq.d/                       1400   0.0
AHO764 (AHO764Q) /CSM/AH/AHO7-C/AHO764Q.Seq.d/                       1396   0.0
AFO686 (AFO686Q) /CSM/AF/AFO6-D/AFO686Q.Seq.d/                       1396   0.0
AFN679 (AFN679Q) /CSM/AF/AFN6-D/AFN679Q.Seq.d/                       1396   0.0
SFI119 (SFI119Q) /CSM/SF/SFI1-A/SFI119Q.Seq.d/                       1394   0.0
AFO479 (AFO479Q) /CSM/AF/AFO4-D/AFO479Q.Seq.d/                       1394   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end, single read.   599  e-167  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74  4e-09  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.    70  6e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70  6e-08  1
BM280519|BM280519.1 ki01h03.y1 Ascaris suum L4 pSPORT1 Zarlenga v1 Ascaris suum cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    68  2e-07  1
AW255071|AW255071.1 ML1365 peppermint glandular trichome Mentha x piperita cDNA, mRNA sequence.    64  3e-06  1
BM315730|BM315730.1 fw64d09.y1 Sugano SJD adult male Danio rerio cDNA clone 5612584 5' similar to SW:ER60_HUMAN P30101 PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR ;, mRNA sequence.    62  1e-05  1
BU795021|S34275 protein disulfide-isomerase homolog precursor - fluke  (Schistosoma mansoni), mRNA sequence.    60  5e-05  1
BM174369|PDA4_MOUSE PROTEIN DISULFIDE ISOMERASE A4 PRECURSOR (PROTEIN ERP-72) (ERP72), mRNA sequence.    60  5e-05  1
BU791776|S34275 protein disulfide-isomerase homolog precursor - fluke  (Schistosoma mansoni), mRNA sequence.    60  5e-05  1
dna update 2003. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   180   7e-44
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   177   4e-43
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   173   8e-42
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   171   3e-41
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   168   3e-40
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   163   8e-39
CR382131_125(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...   155   2e-36
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   3e-36
(Q10057) RecName: Full=Putative protein disulfide-isomerase C1F5...   152   1e-35
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   3e-35
protein update 2009. 4.25
PSORT 

 psg: 0.99  gvh: 0.67  alm: 0.43  top: 0.23  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 20.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for AFN587 is exc
5' end seq. ID AFN587F
5' end seq. 
>AFN587F.Seq
TGAATAAATTTTTAGCTTTGTTATTTGTTTTAGCTTTGTTNGCTAATATTGCTTTTTCAT
GNGAAGGNCACCCAGAACATGATCATGGGGATGGGGATCATGANCATGATCATGATGAAT
CATTTGTTAAAATNTTANATTCTGATAATTTCCATAATTCAGTTTNTGAACATGACGTAA
CTNTTGTTNTGTTTTATGCCCCATGG----------
Length of 5' end seq. 206
3' end seq. ID AFN587Z
3' end seq. 
>AFN587Z.Seq
----------TTATTTATTCATTGACTCCACTCAAGATAACACTCAAGTTCTCGAAGACG
TAAAGAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGA
AATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTG
TTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCG
ATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAAT
CTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCA
AAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCG
GTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTG
AATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAA
TTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTT
ATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCA
TTGAATTCAAATTACCATCATCTCAAACTGATGATAATGTTAATCTAAAAAAGATTCTTC
TGCTAAACA
Length of 3' end seq. 719
Connected seq. ID AFN587P
Connected seq. 
>AFN587P.Seq
TGAATAAATTTTTAGCTTTGTTATTTGTTTTAGCTTTGTTNGCTAATATTGCTTTTTCAT
GNGAAGGNCACCCAGAACATGATCATGGGGATGGGGATCATGANCATGATCATGATGAAT
CATTTGTTAAAATNTTANATTCTGATAATTTCCATAATTCAGTTTNTGAACATGACGTAA
CTNTTGTTNTGTTTTATGCCCCATGG----------TTATTTATTCATTGACTCCACTCA
AGATAACACTCAAGTTCTCGAAGACGTAAAGAAAATCGCTACCTCACAAAAAGGTAATGC
CGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTC
TGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTT
CGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGG
TGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGT
TAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTT
GGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAA
ATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTC
AAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGC
TGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGT
TGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGATGA
TAATGTTAATCTAAAAAAGATTCTTCTGCTAAACA
Length of connected seq. 925
Full length Seq ID -
Full length Seq. -
Length of full length seq. -