>VHC756 (VHC756Q) /CSM/VH/VHC7-C/VHC756Q.Seq.d/
AGGTGATCGTTTATTAGCAAAAGGTTTTTCAACACCATTCTTGCATGTTGGTTATTCATA
TGTATATTCAAAACTTGAAAGTGCAATGGAAGCAGTTAAAGCAATTGGTGAAGAGATTTC
ACAAGTTGGTTTACCAGATGACCTTTTACCATTTACTTTTGCTTTTACTTCAGATGGTGC
TGTATCACAAGGTGCACTTAAAATCTTTAAATTATTACCACATAAAATGGTTACACCAGA
TGAAATGGTTGAATTAGTTAAAAATAAGAAGGGAGAAAGAGGTATTTTATATGGTACAAT
TATTACATCAGAACACATGGCAGCACCAATTGATCCAGAAAAGAAATTTAATAAAGCTGA
ATATTATGCAGATCCAAGTAAATATAAACCAATCTTTTGTGAGAAATATGCACCATACAT
ATCATGTATTATCAATTGTATGTATTGGGATGCCAAATATCCACGTTTAATCACAATTAG
ACAAATGGAAGAATTGGTTGAAACTAACAATAGTAGATTGATTGGTGTTGCTGATATCTC
TGCCGATGTAAATGGTTCATTGGAATTCTTAATGACXXXXXXXXXXATCTTACCAAGACC
TATGGTATTGAGAGTGTAAATACTCTTTTCCGTGGTACCATCCGTTATAAAGGTTTCTGT
CAAGTCATGGAAGCTGCCGTCGAAATTGGTCTATTGGATACCACTAGCAAATCACATCTC
GCCCCATCCGCCCCACCAATGTCATGGAATCTCGCAATGCGTCATATCTTACCAATTCCT
CAAAGTGAAGGTATTCCAACTCAAGAACTTTTAAGAAGAAAACTTAGAACTAGAAGAGGT
TATCCTTCAAAAGGCTATGACGATGATAAGATTACCTATATCCTTTACGTCTTTGAGTGG
TTAGATATCTTTAGTGAAAAGAATAATGTCGCTCAATGTGGTAACTATTTCGAAGCTTTC
ACAGAGTTATTGAAAACTAAACTCAATCTCTTACCATCAGAAAGAGATTTAATCATTTTA
CATAATGTCGTTGGTATCGAATGGGATGGTGGTAAACATGAAACTAAAACTTCAACACTC
GTTTACTATGGTTCAAAAGATCAATCTGCTGTCAGCACTGTAGTTGGTTTACCAGTTGCT
ATCGCAGCTGAATTATTGGTTGAAAATCAAATTAAAGAAGTTGGTGCAATTAGACCAATC
ACTAAAGAATTTTATAAACCAATGTTAAAANC

GDRLLAKGFSTPFLHVGYSYVYSKLESAMEAVKAIGEEISQVGLPDDLLPFTFAFTSDGA
VSQGALKIFKLLPHKMVTPDEMVELVKNKKGERGILYGTIITSEHMAAPIDPEKKFNKAE
YYADPSKYKPIFCEKYAPYISCIINCMYWDAKYPRLITIRQMEELVETNNSRLIGVADIS
ADVNGSLEFLM---

---LTKTYGIESVNTLFRGTIRYKGFCQVMEAAVEIGLLDTTSKSHLAPSAPPMSWNLAM
RHILPIPQSEGIPTQELLRRKLRTRRGYPSKGYDDDKITYILYVFEWLDIFSEKNNVAQC
GNYFEAFTELLKTKLNLLPSERDLIILHNVVGIEWDGGKHETKTSTLVYYGSKDQSAVST
VVGLPVAIAAELLVENQIKEVGAIRPITKEFYKPMLK

Frame A:
r*sfiskrffntilacwlficifkt*kcngss*snw*rdftswftr*pftiyfcfyfrwc
citrct*nl*iitt*ngytr*ng*is*k*egrkryfiwynyyirthgstn*srkei**s*
ilcrsk*i*tnll*eictihimyyqlyvlgcqistfnhn*tngrig*n*q**idwcc*yl
crckwfigilnd---

---ilprpmvlrv*ilfsvvpsvikvsvkswklpsklvywiplanhisphpphqchgisq
cvisyqflkvkvfqlknf*eenleleevilqkamtmirlpisftslsg*islvkrimsln
vvtisklsqsy*klnsisyhqkei*sfyimslvsngmvvnmklklqhsftmvqkinllsa
l*lvyqllsqlnywlkiklkklvqldqslknfinqc*x

Frame B:
GDRLLAKGFSTPFLHVGYSYVYSKLESAMEAVKAIGEEISQVGLPDDLLPFTFAFTSDGA
VSQGALKIFKLLPHKMVTPDEMVELVKNKKGERGILYGTIITSEHMAAPIDPEKKFNKAE
YYADPSKYKPIFCEKYAPYISCIINCMYWDAKYPRLITIRQMEELVETNNSRLIGVADIS
ADVNGSLEFLM---

---syqdlwy*eckysfpwyhpl*rflsshgscrrnwsigyh*qitsrpirptnvmesrn
asyltnssk*rysnsrtfkkkt*n*krlsfkrl*r**dylyplrl*vvryl**ke*crsm
w*lfrsfhrvien*tqsltirkrfnhft*crwyrmgww*t*n*nfntrllwfkrsiccqh
cswftscyrs*iig*ksn*rswcn*tnh*ril*tnvkx

Frame C:
vivy*qkvfqhhscmlvihmyiqnlkvqwkqlkqlvkrfhklvyqmtfyhllllllqmvl
yhkvhlkslnyyhikwlhqmkwln*lkirrekevfymvqllhqntwqhqliqkrnlikln
imqiqvninqsfvrnmhhtyhvlsivcigmpnihv*sqldkwknwlkltivd*lvllisl
pm*mvhwns**---

---LTKTYGIESVNTLFRGTIRYKGFCQVMEAAVEIGLLDTTSKSHLAPSAPPMSWNLAM
RHILPIPQSEGIPTQELLRRKLRTRRGYPSKGYDDDKITYILYVFEWLDIFSEKNNVAQC
GNYFEAFTELLKTKLNLLPSERDLIILHNVVGIEWDGGKHETKTSTLVYYGSKDQSAVST
VVGLPVAIAAELLVENQIKEVGAIRPITKEFYKPMLK

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC756 (VHC756Q) /CSM/VH/VHC7-C/VHC756Q.Seq.d/                       2379   0.0
VHI192 (VHI192Q) /CSM/VH/VHI1-D/VHI192Q.Seq.d/                       1269   0.0
SHD787 (SHD787Q) /CSM/SH/SHD7-D/SHD787Q.Seq.d/                       1269   0.0
AFN810 (AFN810Q) /CSM/AF/AFN8-A/AFN810Q.Seq.d/                       1269   0.0
VHE481 (VHE481Q) /CSM/VH/VHE4-D/VHE481Q.Seq.d/                       1263   0.0
AHL713 (AHL713Q) /CSM/AH/AHL7-A/AHL713Q.Seq.d/                       1263   0.0
AHL574 (AHL574Q) /CSM/AH/AHL5-D/AHL574Q.Seq.d/                       1257   0.0
SHJ770 (SHJ770Q) /CSM/SH/SHJ7-C/SHJ770Q.Seq.d/                       1152   0.0
VHB321 (VHB321Q) /CSM/VH/VHB3-A/VHB321Q.Seq.d/                       1138   0.0
SHK518 (SHK518Q) /CSM/SH/SHK5-A/SHK518Q.Seq.d/                       1124   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AB066287|AB066287.1 Colwellia maris icl gene for isocitrate lyase, complete cds.    50   0.15   1
AL445565|AL445565.1 Mycoplasma pulmonis (strain UAB CTIP) complete genome; segment 3/3.    48   0.57   8
CO648230|CO648230.1 ILLUMIGEN_MCQ_41537 Katze_MMPB2 Macaca mulatta cDNA clone IBIUW:23054 5' similar to Bases 5 to 948 highly similar to human RAB2B (Hs.22399), mRNA sequence.    48   0.60   1
EB516459|EB516459.1 289437 Pigtailed macaque ovary library Macaca nemestrina cDNA 3', mRNA sequence.    48   0.60   1
BV166645|BV166645.1 RAB2B_1055 Rhesus macaque genomic DNA Macaca mulatta STS genomic clone MMA1055, sequence tagged site.    48   0.60   1
AC162040|AC162040.2 Bos taurus clone CH240-114G22, WORKING DRAFT SEQUENCE, 12 unordered pieces.    38   1.4    5
AM620739|AM620739.1 Entamoeba invadens IP1 GSS, clone inv131a09.p1k.    34   1.6    2
AM614949|AM614949.1 Entamoeba invadens IP1 GSS, clone inv054d01.p1k.    34   1.6    2
CK548414|CK548414.1 swkz0_003365.z1 swk Bombyx mori cDNA, mRNA sequence.    46   2.4    1
CF878215|CF878215.1 tric016xp13.g2 T.reesei mycelial culture, Version 6 October 2003 Hypocrea jecorina cDNA clone tric016xp13, mRNA sequence.    46   2.4    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AP008208_2746(AP008208|pid:none) Oryza sativa (japonica cultivar...   188   4e-46
U95758_1(U95758|pid:none) Arabidopsis thaliana lysine-ketoglutar...   183   9e-45
U90522_1(U90522|pid:none) Arabidopsis thaliana lysine-ketoglutar...   182   2e-44
(Q9SMZ4) RecName: Full=Alpha-aminoadipic semialdehyde synthase; ...   182   2e-44
AF264147_1(AF264147|pid:none) Gossypium hirsutum monofunctional ...   179   2e-43
AF264146_1(AF264146|pid:none) Gossypium hirsutum bifunctional ly...   178   3e-43
BT062000_1(BT062000|pid:none) Zea mays full-length cDNA clone ZM...   176   2e-42
AK064521_1(AK064521|pid:none) Oryza sativa Japonica Group cDNA c...   165   3e-39
T02930(T02930)lysine-ketoglutarate reductase / saccharopine dehy...   150   1e-34
(A8E657) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...   127   8e-28

 psg: 0.87  gvh: 0.52  alm: 0.28  top: 0.43  tms: 0.07  mit: 0.31  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.10  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: vesicles of secretory system
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum
	  4.0 %: vacuolar

>> prediction for VHC756 is cyt

>VHC756F.Seq
AGGTGATCGTTTATTAGCAAAAGGTTTTTCAACACCATTCTTGCATGTTGGTTATTCATA
TGTATATTCAAAACTTGAAAGTGCAATGGAAGCAGTTAAAGCAATTGGTGAAGAGATTTC
ACAAGTTGGTTTACCAGATGACCTTTTACCATTTACTTTTGCTTTTACTTCAGATGGTGC
TGTATCACAAGGTGCACTTAAAATCTTTAAATTATTACCACATAAAATGGTTACACCAGA
TGAAATGGTTGAATTAGTTAAAAATAAGAAGGGAGAAAGAGGTATTTTATATGGTACAAT
TATTACATCAGAACACATGGCAGCACCAATTGATCCAGAAAAGAAATTTAATAAAGCTGA
ATATTATGCAGATCCAAGTAAATATAAACCAATCTTTTGTGAGAAATATGCACCATACAT
ATCATGTATTATCAATTGTATGTATTGGGATGCCAAATATCCACGTTTAATCACAATTAG
ACAAATGGAAGAATTGGTTGAAACTAACAATAGTAGATTGATTGGTGTTGCTGATATCTC
TGCCGATGTAAATGGTTCATTGGAATTCTTAATGACNNNNNNNNNN

>VHC756Z.Seq
NNNNNNNNNNATCTTACCAAGACCTATGGTATTGAGAGTGTAAATACTCTTTTCCGTGGT
ACCATCCGTTATAAAGGTTTCTGTCAAGTCATGGAAGCTGCCGTCGAAATTGGTCTATTG
GATACCACTAGCAAATCACATCTCGCCCCATCCGCCCCACCAATGTCATGGAATCTCGCA
ATGCGTCATATCTTACCAATTCCTCAAAGTGAAGGTATTCCAACTCAAGAACTTTTAAGA
AGAAAACTTAGAACTAGAAGAGGTTATCCTTCAAAAGGCTATGACGATGATAAGATTACC
TATATCCTTTACGTCTTTGAGTGGTTAGATATCTTTAGTGAAAAGAATAATGTCGCTCAA
TGTGGTAACTATTTCGAAGCTTTCACAGAGTTATTGAAAACTAAACTCAATCTCTTACCA
TCAGAAAGAGATTTAATCATTTTACATAATGTCGTTGGTATCGAATGGGATGGTGGTAAA
CATGAAACTAAAACTTCAACACTCGTTTACTATGGTTCAAAAGATCAATCTGCTGTCAGC
ACTGTAGTTGGTTTACCAGTTGCTATCGCAGCTGAATTATTGGTTGAAAATCAAATTAAA
GAAGTTGGTGCAATTAGACCAATCACTAAAGAATTTTATAAACCAATGTTAAAANC

>VHC756P.Seq
AGGTGATCGTTTATTAGCAAAAGGTTTTTCAACACCATTCTTGCATGTTGGTTATTCATA
TGTATATTCAAAACTTGAAAGTGCAATGGAAGCAGTTAAAGCAATTGGTGAAGAGATTTC
ACAAGTTGGTTTACCAGATGACCTTTTACCATTTACTTTTGCTTTTACTTCAGATGGTGC
TGTATCACAAGGTGCACTTAAAATCTTTAAATTATTACCACATAAAATGGTTACACCAGA
TGAAATGGTTGAATTAGTTAAAAATAAGAAGGGAGAAAGAGGTATTTTATATGGTACAAT
TATTACATCAGAACACATGGCAGCACCAATTGATCCAGAAAAGAAATTTAATAAAGCTGA
ATATTATGCAGATCCAAGTAAATATAAACCAATCTTTTGTGAGAAATATGCACCATACAT
ATCATGTATTATCAATTGTATGTATTGGGATGCCAAATATCCACGTTTAATCACAATTAG
ACAAATGGAAGAATTGGTTGAAACTAACAATAGTAGATTGATTGGTGTTGCTGATATCTC
TGCCGATGTAAATGGTTCATTGGAATTCTTAATGAC----------ATCTTACCAAGACC
TATGGTATTGAGAGTGTAAATACTCTTTTCCGTGGTACCATCCGTTATAAAGGTTTCTGT
CAAGTCATGGAAGCTGCCGTCGAAATTGGTCTATTGGATACCACTAGCAAATCACATCTC
GCCCCATCCGCCCCACCAATGTCATGGAATCTCGCAATGCGTCATATCTTACCAATTCCT
CAAAGTGAAGGTATTCCAACTCAAGAACTTTTAAGAAGAAAACTTAGAACTAGAAGAGGT
TATCCTTCAAAAGGCTATGACGATGATAAGATTACCTATATCCTTTACGTCTTTGAGTGG
TTAGATATCTTTAGTGAAAAGAATAATGTCGCTCAATGTGGTAACTATTTCGAAGCTTTC
ACAGAGTTATTGAAAACTAAACTCAATCTCTTACCATCAGAAAGAGATTTAATCATTTTA
CATAATGTCGTTGGTATCGAATGGGATGGTGGTAAACATGAAACTAAAACTTCAACACTC
GTTTACTATGGTTCAAAAGATCAATCTGCTGTCAGCACTGTAGTTGGTTTACCAGTTGCT
ATCGCAGCTGAATTATTGGTTGAAAATCAAATTAAAGAAGTTGGTGCAATTAGACCAATC
ACTAAAGAATTTTATAAACCAATGTTAAAANC