>AFN582 (AFN582Q) /CSM/AF/AFN5-D/AFN582Q.Seq.d/
AAAATATTTTCACACACATACATCAACAACAACCACCACAAATATACAATGGATGTCGAT
GACTCTTTACACCAAGATACTTTGGATGATAAATTTTATTCAATTTTAAAAAATATTTCA
TCACCAAATAGACAAGAAAGATATGAAGCAGTTCAATGTGTTAGAAAAATTTTATCTGTT
GAAAATAATCCACCAATTGATAGAGTTATTAGTTCAGGATTAGCACCAATTTTACATAGA
TATTTAGTTGATGAAACAGATACATCAATTCAATATGAAGCATCATGGGCATTAACAAAT
GTTGCATCAGGATCATCAGAACATACAAATTATATTGTAGAGAATGAAATTCATAAGAGT
ATAATTGATTTATTATCAACCAGTAATAATATTGACTTTTTAGAACAAAACATTTGGGCA
TTAGGTAATATCATTGGTGATTCATCACAAAATAGAGATAGTATACTCAACGAGGGTATA
GTATTACATTTTGGTGGAGATATTGAAAACTCATACAAAGAAATCACTAGTTTCAAATAT
AAGTTGGACCATTAGTAATCTATGTCGTGGCACACCACGTCCAGCATTTAGATTCATTAA
GCCACTATTACCATXXXXXXXXXXGTTATTGGTGTTTGTCAAATATTACCAGTGGTCCAT
CGAGTCAAATTCAAAGAGTCATAGACGAGGGTTTGGTTAAAAGAGCTATTGAAACCGGTA
AAAAGCCAACAATCAATATGGACACTAAGCGTGAAATCACTTGGGCTATCATTAACGCTA
TCACTGGTGCTTCCAATCCACAATCGCTCTACTTGATACAGAATGATTGTCTTGATTGTT
TGAAAAAGAATTATTCAACAGCTCAAGCTCAATCAAATCTATTTCATTACTATTTGGATG
GCATTACCAATATATTAAAATTGGTCAATAAATATACCAAAGAAACAAACTGTTTAAAAC
TTTTCAACACTTTTATCGATGATGAATTCTTTGGTACTTTAATTGACGATATCAATTCTT
GTTCACCAGAATTTGTCGAAAATTACTCGTCAATCAATTCTGCACATTGCGAGTTGAAAA
AAGTCGTTAAACATATTATTGAAATTAAAAATTCAAAAGGTGAAAATGTAAATGAAGTTT
TTTTAAAAAGTGGTTTTGAAAATATAAAAAAAGTATTGAATTTGAATCTGATTTAGATGA
GGATGAAATTGATTGTGAATCTGTCGAAGAAGAAGAGGAAGAAGAAGAAGAAGAAGAAGC
TTCTGATTATGAAGATGATATTGCTAAATTAAACGAAATGGATCTTAAGAATAAAAAATA
AAATAAAATAAAATAAAATAAAATAAATAAATAAAATTCAA

kyfhthtstttttniqwmsmtlytkilwminfiqf*kifhhqidkkdmkqfnvlekfyll
kiihqliellvqd*hqfyidi*lmkqihqfnmkhhgh*qmlhqdhqniqiil*rmkfirv
*liyyqpviiltf*nktfgh*vislvihhkieivystrv*YYILVEILKTHTKKSLVSNI
SWTISNLCRGTPRPAFRFIKPLLP---

---YWCLSNITSGPSSQIQRVIDEGLVKRAIETGKKPTINMDTKREITWAIINAITGASN
PQSLYLIQNDCLDCLKKNYSTAQAQSNLFHYYLDGITNILKLVNKYTKETNCLKLFNTFI
DDEFFGTLIDDINSCSPEFVENYSSINSAHCELKKVVKHIIEIKNSKGENVNEVFLKSGF
ENIKKVLNLNLI*mrmklivnlskkkrkkkkkkkllimkmilln*tkwilriknkik*nk
ik*inkiq

Frame A:
kifshtyinnnhhkytmdvddslhqdtlddkfysilknisspnrqeryeavqcvrkilsv
ennppidrvissglapilhrylvdetdtsiqyeaswaltnvasgssehtnyiveneihks
iidllstsnnidfleqniwalgniigdssqnrdsilnegivlhfggdiensykeitsfky
kldh**smswhttssi*ih*atit---

---vigvcqilpvvhrvkfkes*trvwlkellkpvksqqsiwtlsvkslglsltlslvlp
ihnrst*yrmivliv*kriiqqlklnqiyfitiwmalpiy*nwsinipkkqtv*nfstll
smmnslvl*ltisilvhqnlskitrqsilhias*kkslnillklkiqkvkm*mkff*kvv
lki*kky*i*i*fr*g*n*l*icrrrrgrrrrrrsf*l*r*yc*ikrngs*e*kik*nki
k*nk*ikf

Frame B:
kyfhthtstttttniqwmsmtlytkilwminfiqf*kifhhqidkkdmkqfnvlekfyll
kiihqliellvqd*hqfyidi*lmkqihqfnmkhhgh*qmlhqdhqniqiil*rmkfirv
*liyyqpviiltf*nktfgh*vislvihhkieivystrv*YYILVEILKTHTKKSLVSNI
SWTISNLCRGTPRPAFRFIKPLLP---

---llvfvkyyqwsiesnskshrrgfg*ksy*nr*kannqygh*a*nhlgyh*ryhwcfq
stialldte*ls*lfekelfnssssiksisllfgwhyqyikigq*iyqrnklfktfqhfy
r**ilwyfn*ryqflftricrkllvnqfctlrveksr*tyy*n*kfkr*kck*sffkkwf
*kykksiefesdldedeidcesveeeeeeeeeeeasdyeddiaklnemdlknkk*nkik*
nkink*ns

Frame C:
nifthihqqqppqiyngcr*lftpryfg**ilfnfkkyfitk*trki*sssmc*knfic*
k*stn**sy*fristnft*ifs**nryinsi*simginkcciriirtyklycre*ns*ey
n*fiinq**y*lfrtkhlgir*yhw*fitk*r*ytqrgysitfwwry*kliqrnh*fqi*
vgplviyvvahhvqhldslshyyh---

---YWCLSNITSGPSSQIQRVIDEGLVKRAIETGKKPTINMDTKREITWAIINAITGASN
PQSLYLIQNDCLDCLKKNYSTAQAQSNLFHYYLDGITNILKLVNKYTKETNCLKLFNTFI
DDEFFGTLIDDINSCSPEFVENYSSINSAHCELKKVVKHIIEIKNSKGENVNEVFLKSGF
ENIKKVLNLNLI*mrmklivnlskkkrkkkkkkkllimkmilln*tkwilriknkik*nk
ik*inkiq

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFN582 (AFN582Q) /CSM/AF/AFN5-D/AFN582Q.Seq.d/                       2286   0.0
VFO535 (VFO535Q) /CSM/VF/VFO5-B/VFO535Q.Seq.d/                       1176   0.0
SFG848 (SFG848Q) /CSM/SF/SFG8-B/SFG848Q.Seq.d/                       1126   0.0
VFJ837 (VFJ837Q) /CSM/VF/VFJ8-B/VFJ837Q.Seq.d/                       1120   0.0
SFG618 (SFG618Q) /CSM/SF/SFG6-A/SFG618Q.Seq.d/                       1066   0.0
VFM461 (VFM461Q) /CSM/VF/VFM4-C/VFM461Q.Seq.d/                        967   0.0
VFJ656 (VFJ656Q) /CSM/VF/VFJ6-C/VFJ656Q.Seq.d/                        938   0.0
SLK645 (SLK645Q) /CSM/SL/SLK6-B/SLK645Q.Seq.d/                         62   2e-08
SLC533 (SLC533Q) /CSM/SL/SLC5-B/SLC533Q.Seq.d/                         62   2e-08
CHI254 (CHI254Q) /CSM/CH/CHI2-C/CHI254Q.Seq.d/                         62   2e-08

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.   272  e-158  11
AC115680|AC115680.2 Dictyostelium discoideum chromosome 2 map 4415041-4520361 strain AX4, complete sequence.    56  5e-04  7
AF369706|AF369706.1 Capsicum annuum importin alpha 1 mRNA, complete cds.    52  0.002  2
BE341239|BE341239.1 EST345340 potato stolon, Cornell University Solanum tuberosum cDNA clone cSTA16L1, mRNA sequence.    52  0.019  1
BG599238|BG599238.1 EST504138 cSTS Solanum tuberosum cDNA clone cSTS23D12 5' sequence, mRNA sequence.    52  0.019  1
BJ553709|BJ553709.1 Ipomoea nil cDNA clone:jm11o05, 5' end, single read.    52  0.019  1
AW906877|AW906877.1 EST343000 potato stolon, Cornell University Solanum tuberosum cDNA clone cSTA4F14, mRNA sequence.    52  0.019  1
BG599294|BG599294.1 EST504194 cSTS Solanum tuberosum cDNA clone cSTS23N6 5' sequence, mRNA sequence.    52  0.019  1
AW429045|AW429045.1 EST306417 tomato flower buds 0-3 mm, Cornell University Lycopersicon esculentum cDNA clone cTOA1G18 5', mRNA sequence.    52  0.019  1
AL929352|AL929352.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 2/4.    40  0.072  13

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF369706_1(AF369706|pid:none) Capsicum annuum importin alpha 1 m...   103   1e-34
AC116984_59(AC116984|pid:none) Dictyostelium discoideum chromoso...   149   3e-34
A86157(A86157) probable importin alpha subunit [imported] - Arab...   107   1e-32
AC003114_2(AC003114|pid:none) Arabidopsis thaliana chromosome 1 ...   102   2e-31
T52101(T52101) probable nuclear transport factor importin alpha-...   102   2e-31
EF153481_1(EF153481|pid:none) Brassica napus importin alpha-like...   103   3e-31
(Q76P29) RecName: Full=Importin subunit alpha-B; AltName: Full=K...   100   3e-31
AE014296_3413(AE014296|pid:none) Drosophila melanogaster chromos...   103   2e-30
Y09511_1(Y09511|pid:none) A.thaliana mRNA for Kap alpha protein.      110   2e-30
(O04294) RecName: Full=Importin subunit alpha-2; AltName: Full=K...   110   4e-30

 psg: 0.63  gvh: 0.51  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.49  mip: 0.11
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: mitochondrial
	  8.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: cytoplasmic
	  4.0 %: vesicles of secretory system

>> prediction for AFN582 is mit

>AFN582F.Seq
AAAATATTTTCACACACATACATCAACAACAACCACCACAAATATACAATGGATGTCGAT
GACTCTTTACACCAAGATACTTTGGATGATAAATTTTATTCAATTTTAAAAAATATTTCA
TCACCAAATAGACAAGAAAGATATGAAGCAGTTCAATGTGTTAGAAAAATTTTATCTGTT
GAAAATAATCCACCAATTGATAGAGTTATTAGTTCAGGATTAGCACCAATTTTACATAGA
TATTTAGTTGATGAAACAGATACATCAATTCAATATGAAGCATCATGGGCATTAACAAAT
GTTGCATCAGGATCATCAGAACATACAAATTATATTGTAGAGAATGAAATTCATAAGAGT
ATAATTGATTTATTATCAACCAGTAATAATATTGACTTTTTAGAACAAAACATTTGGGCA
TTAGGTAATATCATTGGTGATTCATCACAAAATAGAGATAGTATACTCAACGAGGGTATA
GTATTACATTTTGGTGGAGATATTGAAAACTCATACAAAGAAATCACTAGTTTCAAATAT
AAGTTGGACCATTAGTAATCTATGTCGTGGCACACCACGTCCAGCATTTAGATTCATTAA
GCCACTATTACCAT----------

>AFN582Z.Seq
----------GTTATTGGTGTTTGTCAAATATTACCAGTGGTCCATCGAGTCAAATTCAA
AGAGTCATAGACGAGGGTTTGGTTAAAAGAGCTATTGAAACCGGTAAAAAGCCAACAATC
AATATGGACACTAAGCGTGAAATCACTTGGGCTATCATTAACGCTATCACTGGTGCTTCC
AATCCACAATCGCTCTACTTGATACAGAATGATTGTCTTGATTGTTTGAAAAAGAATTAT
TCAACAGCTCAAGCTCAATCAAATCTATTTCATTACTATTTGGATGGCATTACCAATATA
TTAAAATTGGTCAATAAATATACCAAAGAAACAAACTGTTTAAAACTTTTCAACACTTTT
ATCGATGATGAATTCTTTGGTACTTTAATTGACGATATCAATTCTTGTTCACCAGAATTT
GTCGAAAATTACTCGTCAATCAATTCTGCACATTGCGAGTTGAAAAAAGTCGTTAAACAT
ATTATTGAAATTAAAAATTCAAAAGGTGAAAATGTAAATGAAGTTTTTTTAAAAAGTGGT
TTTGAAAATATAAAAAAAGTATTGAATTTGAATCTGATTTAGATGAGGATGAAATTGATT
GTGAATCTGTCGAAGAAGAAGAGGAAGAAGAAGAAGAAGAAGAAGCTTCTGATTATGAAG
ATGATATTGCTAAATTAAACGAAATGGATCTTAAGAATAAAAAATAAAATAAAATAAAAT
AAAATAAAATAAATAAATAAAATTCAA

>AFN582P.Seq
AAAATATTTTCACACACATACATCAACAACAACCACCACAAATATACAATGGATGTCGAT
GACTCTTTACACCAAGATACTTTGGATGATAAATTTTATTCAATTTTAAAAAATATTTCA
TCACCAAATAGACAAGAAAGATATGAAGCAGTTCAATGTGTTAGAAAAATTTTATCTGTT
GAAAATAATCCACCAATTGATAGAGTTATTAGTTCAGGATTAGCACCAATTTTACATAGA
TATTTAGTTGATGAAACAGATACATCAATTCAATATGAAGCATCATGGGCATTAACAAAT
GTTGCATCAGGATCATCAGAACATACAAATTATATTGTAGAGAATGAAATTCATAAGAGT
ATAATTGATTTATTATCAACCAGTAATAATATTGACTTTTTAGAACAAAACATTTGGGCA
TTAGGTAATATCATTGGTGATTCATCACAAAATAGAGATAGTATACTCAACGAGGGTATA
GTATTACATTTTGGTGGAGATATTGAAAACTCATACAAAGAAATCACTAGTTTCAAATAT
AAGTTGGACCATTAGTAATCTATGTCGTGGCACACCACGTCCAGCATTTAGATTCATTAA
GCCACTATTACCAT----------GTTATTGGTGTTTGTCAAATATTACCAGTGGTCCAT
CGAGTCAAATTCAAAGAGTCATAGACGAGGGTTTGGTTAAAAGAGCTATTGAAACCGGTA
AAAAGCCAACAATCAATATGGACACTAAGCGTGAAATCACTTGGGCTATCATTAACGCTA
TCACTGGTGCTTCCAATCCACAATCGCTCTACTTGATACAGAATGATTGTCTTGATTGTT
TGAAAAAGAATTATTCAACAGCTCAAGCTCAATCAAATCTATTTCATTACTATTTGGATG
GCATTACCAATATATTAAAATTGGTCAATAAATATACCAAAGAAACAAACTGTTTAAAAC
TTTTCAACACTTTTATCGATGATGAATTCTTTGGTACTTTAATTGACGATATCAATTCTT
GTTCACCAGAATTTGTCGAAAATTACTCGTCAATCAATTCTGCACATTGCGAGTTGAAAA
AAGTCGTTAAACATATTATTGAAATTAAAAATTCAAAAGGTGAAAATGTAAATGAAGTTT
TTTTAAAAAGTGGTTTTGAAAATATAAAAAAAGTATTGAATTTGAATCTGATTTAGATGA
GGATGAAATTGATTGTGAATCTGTCGAAGAAGAAGAGGAAGAAGAAGAAGAAGAAGAAGC
TTCTGATTATGAAGATGATATTGCTAAATTAAACGAAATGGATCTTAAGAATAAAAAATA
AAATAAAATAAAATAAAATAAAATAAATAAATAAAATTCAA