>VHC720 (VHC720Q) /CSM/VH/VHC7-A/VHC720Q.Seq.d/
AGATGAGTAAACCATCAAACACACCACCTCTTCCAAAAGATGGGGAGAGAAATATTTTAA
TCACATCAGCTTTACCATACGTAAATAATGTTCCACATCTTGGTAATATTATTGGTTGTG
TATTAAGTGCAGATGTATATGCAAGATATTGTCGTTTAAAAAATTATAATTGTATTTATA
TTTGTGGTACAGATGAATATGGTACAGCAACAGAAACAAAAGCATTATCAGAAGGATGTA
CACCAAAAGAGATTTGTGATAAATATCATGAAATTCATAAAGAGATTTATGAATGGTTTA
ATATTTCATTTGATAAATTTGGAAGAACCTCAACCAATAGTCAAACTGAGATTGCTCAAG
ATATTTTCAACAAGATTAAAGATAACGGATATACATTAACTCAAGAGATTGAACAACTTT
ATTGTGAACAAACTTGTAAGATGTTTTTAGCAGATCGTTTTGTGGAGGGAACATGTCCAC
ATTGTAAATTTGAGGATGCAAGAGGTGATCAATGTGATGGTTGTTCCAAATTATTGAATC
CAACCGAATTGATCAACCCACGTTGCAAAGTTTGTTCAAAACCACCAGTTATAAAATCAA
CCAAACACATTTTCATCGACTTACCACAATTGCAACAACAAGTXXXXXXXXXXAATCCTC
ATCAATTTAATCAAACTTTTATCAACCCTCTTAGAACCATACATCCCATCACTCACCGAT
AAAGTCCATCAACAATTAAATGTTGAACCAACTAAATATTCCACCCATTTCGACATCAAT
GCAATTCCAGCAGGTCATGAAATTTCAAAGGAAATCTTACCATTGGTAAAGAAAATCGAA
GCCGATGACCTCAAGAAATGGCGTACAAAATTCTCTGGAATCGGACCAGAATTCCCAATC
GATATGAAAATTGCCACAGTCTTGGAGGTAAATGATCATCCATCCGCAGAGAATTTATAT
GTAATTAAATTAAGTTTAGGTGGTGATCAAACTAAAACCGCAGTTTCAGGTATTAAAGCA
AACTTTGAAAAATCCCAACTCATTGGTAAGAAATTGGCTGTAGTATTAAATTTGAAACCT
TCAAAATTCAAAGGTGTTCTCTCTGAAGCAATGATTCTTGTTGCTGATGATGGTCAAGCC
ACTAAAGAAAGTTTATCATTCTTGGTACCATCAAATCCACAATCAATTGAAGCTGGTTCA
AAAATTGCCGGTAAAGGTATGTCAATTAAACCAAAACCAACAATTGATTATCAAAAAGAA
TTCCTTCATTATGATCTCACTTGTATTGATAAAATTATTAGTTATAATAAATCTCAATTA
TTTATCAATCAAAANAACCTTTAGTTTCGAAAAAATGGTAAGGTAAAGTT

MSKPSNTPPLPKDGERNILITSALPYVNNVPHLGNIIGCVLSADVYARYCRLKNYNCIYI
CGTDEYGTATETKALSEGCTPKEICDKYHEIHKEIYEWFNISFDKFGRTSTNSQTEIAQD
IFNKIKDNGYTLTQEIEQLYCEQTCKMFLADRFVEGTCPHCKFEDARGDQCDGCSKLLNP
TELINPRCKVCSKPPVIKSTKHIFIDLPQLQQQ---

---ILINLIKLLSTLLEPYIPSLTDKVHQQLNVEPTKYSTHFDINAIPAGHEISKEILPL
VKKIEADDLKKWRTKFSGIGPEFPIDMKIATVLEVNDHPSAENLYVIKLSLGGDQTKTAV
SGIKANFEKSQLIGKKLAVVLNLKPSKFKGVLSEAMILVADDGQATKESLSFLVPSNPQS
IEAGSKIAGKGMSIKPKPTIDYQKEFLHYDLTCIDKIISYNKSQLFINQXNL*frkngkv
k

Frame A:
r*vnhqthhlfqkmgreif*shqlyht*imfhilvillvvy*vqmymqdivv*kiiivfi
fvvqmnmvqqqkqkhyqkdvhqkrfvinimkfikrfmnglifhlinleepqpivklrllk
ifstrlkitdih*lkrlnnfivnklvrcf*qivlwrehvhivnlrmqevinvmvvpny*i
qpn*sthvakfvqnhql*nqpntfsstyhncnnk---

---nphqfnqtfinplrtihpithr*spstikc*tn*ifhpfrhqcnssrs*nfkgnlti
gkenrsr*pqemaykilwnrtripnryenchslggk*ssirreficn*ikfrw*sn*nrs
fry*skl*kipthw*eigcsikfetfkiqrcsl*sndscc**wssh*rkfiilgtiksti
n*swfkncr*ryvn*tktnn*lskripsl*shly**ny*l**isiiyqskxplvskkw*g
kv

Frame B:
de*tikhttsskrwgekyfnhisftirk*cstsw*yywlcikcrcickilsfkkl*lyly
lwyr*iwysnrnksiirrmytkrdl**is*ns*rdl*mv*yfi**iwknlnq*sn*dcsr
yfqqd*r*riyinsrd*ttll*tnl*dvfsrsfcggnmstl*i*gckr*sm*wlfqiies
nridqptlqslfkttsykinqthfhrlttiatts---

---ILINLIKLLSTLLEPYIPSLTDKVHQQLNVEPTKYSTHFDINAIPAGHEISKEILPL
VKKIEADDLKKWRTKFSGIGPEFPIDMKIATVLEVNDHPSAENLYVIKLSLGGDQTKTAV
SGIKANFEKSQLIGKKLAVVLNLKPSKFKGVLSEAMILVADDGQATKESLSFLVPSNPQS
IEAGSKIAGKGMSIKPKPTIDYQKEFLHYDLTCIDKIISYNKSQLFINQXNL*frkngkv
k

Frame C:
MSKPSNTPPLPKDGERNILITSALPYVNNVPHLGNIIGCVLSADVYARYCRLKNYNCIYI
CGTDEYGTATETKALSEGCTPKEICDKYHEIHKEIYEWFNISFDKFGRTSTNSQTEIAQD
IFNKIKDNGYTLTQEIEQLYCEQTCKMFLADRFVEGTCPHCKFEDARGDQCDGCSKLLNP
TELINPRCKVCSKPPVIKSTKHIFIDLPQLQQQ---

---sssi*snfyqps*nhtshhspiksinn*mlnqlnippistsmqfqqvmkfqrksyhw
*rkskpmtsrngvqnslesdqnsqsi*klpqswr*miihpqriym*ln*v*vviklkpqf
qvlkqtlknpnslvrnwl*y*i*nlqnskvfslkq*flllmmvkplkkvyhswyhqihnq
lklvqklpvkvcqlnqnqqliikknsfimislvlikllviinlnylsikxtfsfekmvr*
s

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC720 (VHC720Q) /CSM/VH/VHC7-A/VHC720Q.Seq.d/                       2650   0.0
VHJ847 (VHJ847Q) /CSM/VH/VHJ8-B/VHJ847Q.Seq.d/                       1400   0.0
VHC493 (VHC493Q) /CSM/VH/VHC4-D/VHC493Q.Seq.d/                       1386   0.0
VHK475 (VHK475Q) /CSM/VH/VHK4-D/VHK475Q.Seq.d/                       1380   0.0
VHA513 (VHA513Q) /CSM/VH/VHA5-A/VHA513Q.Seq.d/                       1358   0.0
AHB519 (AHB519Q) /CSM/AH/AHB5-A/AHB519Q.Seq.d/                       1338   0.0
VHM434 (VHM434Q) /CSM/VH/VHM4-B/VHM434Q.Seq.d/                       1318   0.0
VHM654 (VHM654Q) /CSM/VH/VHM6-C/VHM654Q.Seq.d/                       1306   0.0
AHD434 (AHD434Q) /CSM/AH/AHD4-B/AHD434Q.Seq.d/                       1275   0.0
VHM217 (VHM217Q) /CSM/VH/VHM2-A/VHM217Q.Seq.d/                       1261   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CN761937|CN761937.1 ID0AAA3BE05RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA3BE05 5',mRNA sequence.    80   5e-34  5
EE261858|EE261858.1 F09_F09ff2k18_pDNRf_513965 Myzus persicae, line F001, whole female aphid library Myzus persicae cDNA clone F09_F09ff2k18_pDNRf_513965, mRNA sequence.    72   9e-34  3
EC756229|EC756229.1 PPE00003887 Agencourt Biosciences Agen-0020 Non-normalized library cloned into Not I - Eco RV sites of pAGEN-1 Physarum polycephalum cDNA, mRNA sequence.    92   1e-33  4
DY447875|SYM_HUMAN Methionyl-tRNA synthetase (Methionine--tRNA ligase) (MetRS) >, mRNA sequence.    66   3e-20  5
AR549033|AR549033.1 Sequence 4164 from patent US 6747137.          46   3e-18  6
EL477374|EL477374.1 CHUL5577.b1_A04.ab1 CHU(LMS) puzzle sunflower Helianthus paradoxus cDNA clone CHUL5577, mRNA sequence.    62   4e-17  4
CN560372|CN560372.1 tag32c12.x1 Hydra EST -Kiel 2 Hydra magnipapillata cDNA 3' similar to SW:SYM_HUMAN P56192 METHIONYL-TRNA SYNTHETASE ;, mRNA sequence.    68   2e-16  4
AX489310|AX489310.1 Sequence 6610 from Patent WO02053728.          46   3e-16  6
EH431684|EH431684.1 NPE00000653 Neocallimastix patriciarum ZAP II cDNA library Neocallimastix patriciarum cDNA, mRNA sequence.    46   1e-13  3
BH146733|BH146733.1 ENTQN84TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    50   2e-12  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X95) RecName: Full=Probable methionyl-tRNA synthetase, cytop...   457   e-127
(Q9SVN5) RecName: Full=Probable methionyl-tRNA synthetase;      ...   319   2e-85
BC077353_1(BC077353|pid:none) Xenopus laevis methionine-tRNA syn...   314   5e-84
(Q6PF21) RecName: Full=Methionyl-tRNA synthetase, cytoplasmic;  ...   310   1e-82
BC167909_1(BC167909|pid:none) Xenopus tropicalis cDNA clone MGC:...   309   1e-82
AP008212_1204(AP008212|pid:none) Oryza sativa (japonica cultivar...   308   4e-82
AF040700_1(AF040700|pid:none) Oryza sativa methionyl-tRNA synthe...   308   4e-82
CU928166_373(CU928166|pid:none) Kluyveromyces thermotolerans str...   306   9e-82
AK222767_1(AK222767|pid:none) Homo sapiens mRNA for methionine-t...   306   1e-81
(P56192) RecName: Full=Methionyl-tRNA synthetase, cytoplasmic;  ...   306   1e-81

 psg: 0.74  gvh: 0.59  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi

>> prediction for VHC720 is cyt

>VHC720F.Seq
AGATGAGTAAACCATCAAACACACCACCTCTTCCAAAAGATGGGGAGAGAAATATTTTAA
TCACATCAGCTTTACCATACGTAAATAATGTTCCACATCTTGGTAATATTATTGGTTGTG
TATTAAGTGCAGATGTATATGCAAGATATTGTCGTTTAAAAAATTATAATTGTATTTATA
TTTGTGGTACAGATGAATATGGTACAGCAACAGAAACAAAAGCATTATCAGAAGGATGTA
CACCAAAAGAGATTTGTGATAAATATCATGAAATTCATAAAGAGATTTATGAATGGTTTA
ATATTTCATTTGATAAATTTGGAAGAACCTCAACCAATAGTCAAACTGAGATTGCTCAAG
ATATTTTCAACAAGATTAAAGATAACGGATATACATTAACTCAAGAGATTGAACAACTTT
ATTGTGAACAAACTTGTAAGATGTTTTTAGCAGATCGTTTTGTGGAGGGAACATGTCCAC
ATTGTAAATTTGAGGATGCAAGAGGTGATCAATGTGATGGTTGTTCCAAATTATTGAATC
CAACCGAATTGATCAACCCACGTTGCAAAGTTTGTTCAAAACCACCAGTTATAAAATCAA
CCAAACACATTTTCATCGACTTACCACAATTGCAACAACAAGTNNNNNNNNNN

>VHC720Z.Seq
NNNNNNNNNNAATCCTCATCAATTTAATCAAACTTTTATCAACCCTCTTAGAACCATACA
TCCCATCACTCACCGATAAAGTCCATCAACAATTAAATGTTGAACCAACTAAATATTCCA
CCCATTTCGACATCAATGCAATTCCAGCAGGTCATGAAATTTCAAAGGAAATCTTACCAT
TGGTAAAGAAAATCGAAGCCGATGACCTCAAGAAATGGCGTACAAAATTCTCTGGAATCG
GACCAGAATTCCCAATCGATATGAAAATTGCCACAGTCTTGGAGGTAAATGATCATCCAT
CCGCAGAGAATTTATATGTAATTAAATTAAGTTTAGGTGGTGATCAAACTAAAACCGCAG
TTTCAGGTATTAAAGCAAACTTTGAAAAATCCCAACTCATTGGTAAGAAATTGGCTGTAG
TATTAAATTTGAAACCTTCAAAATTCAAAGGTGTTCTCTCTGAAGCAATGATTCTTGTTG
CTGATGATGGTCAAGCCACTAAAGAAAGTTTATCATTCTTGGTACCATCAAATCCACAAT
CAATTGAAGCTGGTTCAAAAATTGCCGGTAAAGGTATGTCAATTAAACCAAAACCAACAA
TTGATTATCAAAAAGAATTCCTTCATTATGATCTCACTTGTATTGATAAAATTATTAGTT
ATAATAAATCTCAATTATTTATCAATCAAAANAACCTTTAGTTTCGAAAAAATGGTAAGG
TAAAGTT

>VHC720P.Seq
AGATGAGTAAACCATCAAACACACCACCTCTTCCAAAAGATGGGGAGAGAAATATTTTAA
TCACATCAGCTTTACCATACGTAAATAATGTTCCACATCTTGGTAATATTATTGGTTGTG
TATTAAGTGCAGATGTATATGCAAGATATTGTCGTTTAAAAAATTATAATTGTATTTATA
TTTGTGGTACAGATGAATATGGTACAGCAACAGAAACAAAAGCATTATCAGAAGGATGTA
CACCAAAAGAGATTTGTGATAAATATCATGAAATTCATAAAGAGATTTATGAATGGTTTA
ATATTTCATTTGATAAATTTGGAAGAACCTCAACCAATAGTCAAACTGAGATTGCTCAAG
ATATTTTCAACAAGATTAAAGATAACGGATATACATTAACTCAAGAGATTGAACAACTTT
ATTGTGAACAAACTTGTAAGATGTTTTTAGCAGATCGTTTTGTGGAGGGAACATGTCCAC
ATTGTAAATTTGAGGATGCAAGAGGTGATCAATGTGATGGTTGTTCCAAATTATTGAATC
CAACCGAATTGATCAACCCACGTTGCAAAGTTTGTTCAAAACCACCAGTTATAAAATCAA
CCAAACACATTTTCATCGACTTACCACAATTGCAACAACAAGT----------AATCCTC
ATCAATTTAATCAAACTTTTATCAACCCTCTTAGAACCATACATCCCATCACTCACCGAT
AAAGTCCATCAACAATTAAATGTTGAACCAACTAAATATTCCACCCATTTCGACATCAAT
GCAATTCCAGCAGGTCATGAAATTTCAAAGGAAATCTTACCATTGGTAAAGAAAATCGAA
GCCGATGACCTCAAGAAATGGCGTACAAAATTCTCTGGAATCGGACCAGAATTCCCAATC
GATATGAAAATTGCCACAGTCTTGGAGGTAAATGATCATCCATCCGCAGAGAATTTATAT
GTAATTAAATTAAGTTTAGGTGGTGATCAAACTAAAACCGCAGTTTCAGGTATTAAAGCA
AACTTTGAAAAATCCCAACTCATTGGTAAGAAATTGGCTGTAGTATTAAATTTGAAACCT
TCAAAATTCAAAGGTGTTCTCTCTGAAGCAATGATTCTTGTTGCTGATGATGGTCAAGCC
ACTAAAGAAAGTTTATCATTCTTGGTACCATCAAATCCACAATCAATTGAAGCTGGTTCA
AAAATTGCCGGTAAAGGTATGTCAATTAAACCAAAACCAACAATTGATTATCAAAAAGAA
TTCCTTCATTATGATCTCACTTGTATTGATAAAATTATTAGTTATAATAAATCTCAATTA
TTTATCAATCAAAANAACCTTTAGTTTCGAAAAAATGGTAAGGTAAAGTT