>VHC675 (VHC675Q) /CSM/VH/VHC6-D/VHC675Q.Seq.d/
AAGATTTTAGGTAAAGAAAGATTAGGTCGTATTCTTTCATCATTGTATATGATGAGATTT
GATAATAATTCATCAGTTCGTCAAAAGGTATTATTAATTTGGAAATATATTGTTAGTAAT
ACACCAAAGACACTTCGTGAGATTTTACCAACTCTTATTGAAATGATTXXXXXXXXXXTT
GAAAAGAAACCAAAAAGGTGAACAAGCTACACTCGATAAAGCAATCGCCACCATTGTGGA
CTTCCTTTATCAACCTTCCGATGATCTCAGATCAATGGTCGCTCAAACTATTGGTGCCTC
TTCAAAATGTTTTACTTCTCTCACTGAATTGAATCAATTCATTAAAACCAATCTCATCTC
ACCTTCTCAATCAGTACTCTCTCGTTATGGTAAATCATTGGCATTGGGTGAAATTTTCAA
AGCATCTGGTAAGAATTTAATCGATTCTCAATCACCAAATATGCCAACCATCATTAAAAT
CATTCAAACCGATTGTCGTGATGAAAAAGGTCCAATTCGTGAATCCTCTGCTTATTTGGC
TGAAGCTATTTTAGTTGCTTCGCCTTTAACCTATGCAAAGGATTTAGTACCTTCCATTTG
TCATCTCATTGGTGATCAATCATCTTCCGTATCAATTAGTGCTCTCAATGTTATTAAACG
TTTCTGTAAATCAAATCAACAATTATCAAGACAATATTTACGTGATATTGTCGTACCAAC
TATGAATAGATTAAAAGAAAGAACTAATTTACCTCTAAAATTAGCTGCTGAAAGAACTTT
AGTTCATTCACTTCAAATCTTTAAAGAATCCATTGTAATGGATGATTTAATTAAACAATT
AGAATTATCTGGTGATTCATCAATGGCAAATTCTTTAATNATTATCATAAAAGAGTTTTA
A

KILGKERLGRILSSLYMMRFDNNSSVRQKVLLIWKYIVSNTPKTLREILPTLIEMI---

---LKRNQKGEQATLDKAIATIVDFLYQPSDDLRSMVAQTIGASSKCFTSLTELNQFIKT
NLISPSQSVLSRYGKSLALGEIFKASGKNLIDSQSPNMPTIIKIIQTDCRDEKGPIRESS
AYLAEAILVASPLTYAKDLVPSICHLIGDQSSSVSISALNVIKRFCKSNQQLSRQYLRDI
VVPTMNRLKERTNLPLKLAAERTLVHSLQIFKESIVMDDLIKQLELSGDSSMANSLXIII
KEF*

Frame A:
KILGKERLGRILSSLYMMRFDNNSSVRQKVLLIWKYIVSNTPKTLREILPTLIEMI---

---LKRNQKGEQATLDKAIATIVDFLYQPSDDLRSMVAQTIGASSKCFTSLTELNQFIKT
NLISPSQSVLSRYGKSLALGEIFKASGKNLIDSQSPNMPTIIKIIQTDCRDEKGPIRESS
AYLAEAILVASPLTYAKDLVPSICHLIGDQSSSVSISALNVIKRFCKSNQQLSRQYLRDI
VVPTMNRLKERTNLPLKLAAERTLVHSLQIFKESIVMDDLIKQLELSGDSSMANSLXIII
KEF*

Frame B:
rf*vkkd*vvffhhci**dliiihqfvkryy*fgnillvihqrhfvrfyqlllk*---

---*ketkkvnklhsikqspplwtsfinlpmisdqwslkllvplqnvlllsln*inslkp
isshllnqyslvmvnhwhwvkfskhlvri*silnhqicqpslksfkpivvmkkvqfvnpl
liwlklf*llrl*pmqri*ylpfvislvinhlpyqlvlsmllnvsvnqinnyqdniyvil
syql*id*kkeliyl*n*llkel*fihfkslknpl*wmi*lnn*nylvihqwqil*xls*
ksf

Frame C:
dfr*rkirsysfiivydei***fissskgiinleiyc**ytkdts*dftnsy*nd---

---ekkpkr*tsytr*snrhhcglplstfr*sqingrsnywclfkmfyfsh*iesih*nq
shltfsistlslw*iigig*nfqsiw*efnrfsitkyanhh*nhsnrls**krsns*ilc
lfg*syfscfafnlckgfstfhlsshw*siifrin*csqcy*tfl*ikstiiktift*yc
rtnye*ikrkn*ftskisc*knfssftsnl*rihcng*fn*tiriiw*fingkffnxyhk
rvl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC675 (VHC675Q) /CSM/VH/VHC6-D/VHC675Q.Seq.d/                       1721   0.0
VHB245 (VHB245Q) /CSM/VH/VHB2-B/VHB245Q.Seq.d/                       1374   0.0
VHI374 (VHI374Q) /CSM/VH/VHI3-D/VHI374Q.Seq.d/                       1364   0.0
VHA441 (VHA441Q) /CSM/VH/VHA4-B/VHA441Q.Seq.d/                       1364   0.0
SHF505 (SHF505Q) /CSM/SH/SHF5-A/SHF505Q.Seq.d/                       1364   0.0
VHO589 (VHO589Q) /CSM/VH/VHO5-D/VHO589Q.Seq.d/                       1279   0.0
VHK689 (VHK689Q) /CSM/VH/VHK6-D/VHK689Q.Seq.d/                       1275   0.0
VHA755 (VHA755Q) /CSM/VH/VHA7-C/VHA755Q.Seq.d/                       1259   0.0
VHL277 (VHL277Q) /CSM/VH/VHL2-D/VHL277Q.Seq.d/                       1223   0.0
VHF215 (VHF215Q) /CSM/VH/VHF2-A/VHF215Q.Seq.d/                        957   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC119982|AC119982.6 Mus musculus chromosome 19, clone RP24-498L9, complete sequence.    56   0.002  1
AC118724|AC118724.18 Mus musculus, clone RP24-160H20, complete sequence.    56   0.002  1
BH167468|BH167468.1 ENTSH94TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    50   0.11   1
BH163191|BH163191.1 ENTTE33TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    50   0.11   1
AZ531303|AZ531303.1 ENTCH17TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    50   0.11   1
ED460729|ED460729.1 AUAC-aao05g05.g1 Ascaris suum whole genome shotgun library (PMAJ_4 GSS) Ascaris suum genomic, genomic survey sequence.    50   0.11   1
ED450392|ED450392.1 AUAC-aan56c12.g1 Ascaris suum whole genome shotgun library (PMAJ_4 GSS) Ascaris suum genomic, genomic survey sequence.    50   0.11   1
ED393242|ED393242.1 AUAC-aai25f10.g1 Ascaris suum whole genome shotgun library (PMAJ_4 GSS) Ascaris suum genomic, genomic survey sequence.    50   0.11   1
ED186094|ED186094.1 AUAC-aah66a04.g1 Ascaris suum whole genome shotgun library (PMAJ_4 GSS) Ascaris suum genomic, genomic survey sequence.    50   0.11   1
DV445909|DV445909.1 CV01017A2F05.f1 CV01-normalized library Manihot esculenta cDNA clone CV01017A2F05.f1, mRNA sequence.    46   1.7    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54WR2) RecName: Full=Translational activator gcn1; AltName: Fu...   433   e-120
(P33892) RecName: Full=Translational activator GCN1;  &A48126(A4...    55   3e-14
AC146936_30(AC146936|pid:none) Oryza sativa (japonica cultivar-g...    61   2e-13
AP008209_2460(AP008209|pid:none) Oryza sativa (japonica cultivar...    61   3e-12
AC006193_10(AC006193|pid:none) Arabidopsis thaliana chromosome I...    66   3e-12
AF377946_1(AF377946|pid:none) Oryza sativa (japonica cultivar-gr...    61   3e-12
AL954693_2(AL954693|pid:none) Zebrafish DNA sequence from clone ...    65   2e-09
BC168513_1(BC168513|pid:none) Danio rerio cDNA clone IMAGE:69620...    65   2e-09
AC004812_3(AC004812|pid:none) Homo sapiens PAC clone 267D11 from...    64   9e-09
U77700_1(U77700|pid:none) Homo sapiens HsGCN1 mRNA, partial cds.       64   9e-09

 psg: 0.92  gvh: 0.32  alm: 0.43  top: 0.47  tms: 0.00  mit: 0.32  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: nuclear
	 24.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	 16.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VHC675 is nuc

>VHC675F.Seq
AAGATTTTAGGTAAAGAAAGATTAGGTCGTATTCTTTCATCATTGTATATGATGAGATTT
GATAATAATTCATCAGTTCGTCAAAAGGTATTATTAATTTGGAAATATATTGTTAGTAAT
ACACCAAAGACACTTCGTGAGATTTTACCAACTCTTATTGAAATGATTNNNNNNNNNN

>VHC675Z.Seq
NNNNNNNNNNTTGAAAAGAAACCAAAAAGGTGAACAAGCTACACTCGATAAAGCAATCGC
CACCATTGTGGACTTCCTTTATCAACCTTCCGATGATCTCAGATCAATGGTCGCTCAAAC
TATTGGTGCCTCTTCAAAATGTTTTACTTCTCTCACTGAATTGAATCAATTCATTAAAAC
CAATCTCATCTCACCTTCTCAATCAGTACTCTCTCGTTATGGTAAATCATTGGCATTGGG
TGAAATTTTCAAAGCATCTGGTAAGAATTTAATCGATTCTCAATCACCAAATATGCCAAC
CATCATTAAAATCATTCAAACCGATTGTCGTGATGAAAAAGGTCCAATTCGTGAATCCTC
TGCTTATTTGGCTGAAGCTATTTTAGTTGCTTCGCCTTTAACCTATGCAAAGGATTTAGT
ACCTTCCATTTGTCATCTCATTGGTGATCAATCATCTTCCGTATCAATTAGTGCTCTCAA
TGTTATTAAACGTTTCTGTAAATCAAATCAACAATTATCAAGACAATATTTACGTGATAT
TGTCGTACCAACTATGAATAGATTAAAAGAAAGAACTAATTTACCTCTAAAATTAGCTGC
TGAAAGAACTTTAGTTCATTCACTTCAAATCTTTAAAGAATCCATTGTAATGGATGATTT
AATTAAACAATTAGAATTATCTGGTGATTCATCAATGGCAAATTCTTTAATNATTATCAT
AAAAGAGTTTTAA

>VHC675P.Seq
AAGATTTTAGGTAAAGAAAGATTAGGTCGTATTCTTTCATCATTGTATATGATGAGATTT
GATAATAATTCATCAGTTCGTCAAAAGGTATTATTAATTTGGAAATATATTGTTAGTAAT
ACACCAAAGACACTTCGTGAGATTTTACCAACTCTTATTGAAATGATT----------TT
GAAAAGAAACCAAAAAGGTGAACAAGCTACACTCGATAAAGCAATCGCCACCATTGTGGA
CTTCCTTTATCAACCTTCCGATGATCTCAGATCAATGGTCGCTCAAACTATTGGTGCCTC
TTCAAAATGTTTTACTTCTCTCACTGAATTGAATCAATTCATTAAAACCAATCTCATCTC
ACCTTCTCAATCAGTACTCTCTCGTTATGGTAAATCATTGGCATTGGGTGAAATTTTCAA
AGCATCTGGTAAGAATTTAATCGATTCTCAATCACCAAATATGCCAACCATCATTAAAAT
CATTCAAACCGATTGTCGTGATGAAAAAGGTCCAATTCGTGAATCCTCTGCTTATTTGGC
TGAAGCTATTTTAGTTGCTTCGCCTTTAACCTATGCAAAGGATTTAGTACCTTCCATTTG
TCATCTCATTGGTGATCAATCATCTTCCGTATCAATTAGTGCTCTCAATGTTATTAAACG
TTTCTGTAAATCAAATCAACAATTATCAAGACAATATTTACGTGATATTGTCGTACCAAC
TATGAATAGATTAAAAGAAAGAACTAATTTACCTCTAAAATTAGCTGCTGAAAGAACTTT
AGTTCATTCACTTCAAATCTTTAAAGAATCCATTGTAATGGATGATTTAATTAAACAATT
AGAATTATCTGGTGATTCATCAATGGCAAATTCTTTAATNATTATCATAAAAGAGTTTTA
A