>AFB604 (AFB604Q) /CSM/AF/AFB6-A/AFB604Q.Seq.d/
TAGATTATGCCATCAGTAGATTCGAAGCTTGCGATTTAACATCGATCGTTGAATTGGAGA
CCCAAATCGCCAATATCAAACTCACTCACAAACTCTTATCAGAATACTTTGATATAGATC
CATTCGAATCAATCTTTAATGAAATCAATGAGTCCACTTCTTTGGTCTCTTATCATGGTC
GTATTGTACTTCATATTATCTTTGAATTGGTGGCCGATTTCGCTCCAAACTATACCTTCA
ATTCAATCACTCAACGTTTTATAAAGGCTCCATACACTTTCACCGAAGAATTGAAACGTG
ATGCACTTCCAAAAACTAATCCTGTCTTTTTGTTTGGTAATAAATATTTGAATGCTGCCT
ATGCAAACTCGATCGAACTCTACAAGAATTTCATTGGTGTACCACATATTCAATCAATCC
TTAGAATCGTTGGTAAAAAGAATCTTCCTTTGATCGTTGCTGAGGTATTACGTAATATTG
AAATCAAGATCACCAATGTTCTCTCACCATACGTTAGTGAATTACTTCAAGGTATGCCTG
CATCGACTAAATTACCAATCTATGATTATGGTACTGAAGGXXXXXXXXXXTCTTCAATCC
TTAATATTGTTCGTCATGATTGGTCTGGTGCTCCTCCAGATAATGGTGTCATTTGCGTTG
ATTCCTCAACAGAGTTTTATCGTTTATGGAGTGCCCTTCAATTTGTAATCTGTTGGCCAC
TCTCAAATGAAAATGATAAATCTTTCCATGAATTGTTTGGTGATGGTTTAATGTGGGCCG
GTTGTTCATTGATCCATTTCCTTGGTCAACAATATCGTTTCGAATTATTTGATTTCTGTT
ATCACATTTTAAATGTAGAAGAGGCTGCTGCTGTTCGTTCAGATAAACCTGCCTTAAAGA
ATTTCTTTAAGAATGCTCAAATCATCAAAGATATCAATAATCAAATCTTTAGTATACTCA
ATACATATTGTCCACCACCACCACCTTCTAATATGATCCTTTCACCACCTGCTACTGAAC
AAGCTGAACAATTTATAGTTACAACCACTGTTGAAGAACAATCAATTCAACAACAACAAC
AACAACAACAACAACAACAACTTCAACAATCAACTTCAAATTCTGGAAGTAGTTTATCAC
TTTCTGCTGCTGGTGCACCACCTCCACCACCACCACCATCAACAAATGGTTTACCACCTC
CACCACCAA

DYAISRFEACDLTSIVELETQIANIKLTHKLLSEYFDIDPFESIFNEINESTSLVSYHGR
IVLHIIFELVADFAPNYTFNSITQRFIKAPYTFTEELKRDALPKTNPVFLFGNKYLNAAY
ANSIELYKNFIGVPHIQSILRIVGKKNLPLIVAEVLRNIEIKITNVLSPYVSELLQGMPA
STKLPIYDYGTE---

---SSILNIVRHDWSGAPPDNGVICVDSSTEFYRLWSALQFVICWPLSNENDKSFHELFG
DGLMWAGCSLIHFLGQQYRFELFDFCYHILNVEEAAAVRSDKPALKNFFKNAQIIKDINN
QIFSILNTYCPPPPPSNMILSPPATEQAEQFIVTTTVEEQSIQQQQQQQQQQQLQQSTSN
SGSSLSLSAAGAPPPPPPPSTNGLPPPPP

Frame A:
*impsvdsklai*hrslnwrpkspisnsltnsyqntli*ihsnqslmksmspllwslimv
vlyfilslnwwpislqtipsiqslnvl*rlhtlspkn*nvmhfqklilsfclvini*mlp
mqtrsnstrislvyhifnqsleslvkrifl*sllryyvilksrspmfshhtlvnyfkvcl
hrlnyqsmimvlk---

---SSILNIVRHDWSGAPPDNGVICVDSSTEFYRLWSALQFVICWPLSNENDKSFHELFG
DGLMWAGCSLIHFLGQQYRFELFDFCYHILNVEEAAAVRSDKPALKNFFKNAQIIKDINN
QIFSILNTYCPPPPPSNMILSPPATEQAEQFIVTTTVEEQSIQQQQQQQQQQQLQQSTSN
SGSSLSLSAAGAPPPPPPPSTNGLPPPPP

Frame B:
rlchq*irslrfnidr*igdpnrqyqthsqtliril*yrsirinl**nq*vhffgllsws
yctsyyl*iggrfrsklylqfnhstfykgsihfhrriet*ctskn*sclfvw**ifeccl
ckldrtlqefhwcttysinp*nrw*kessfdrc*git*y*nqdhqcsltir**itsryac
id*itnl*lwy*r---

---lqslilfvmiglvllqimvsfalipqqsfivygvpfnl*svghsqmkminlsmnclv
mv*cgpvvh*sislvnnivsnylisvitf*m*krlllfvqinlp*rislrmlksskisii
kslvysihivhhhhlli*sfhhlllnklnnl*lqpllknnqfnnnnnnnnnnnfnnqlqi
levvyhflllvhhlhhhhhqqmvyhlhhq

Frame C:
DYAISRFEACDLTSIVELETQIANIKLTHKLLSEYFDIDPFESIFNEINESTSLVSYHGR
IVLHIIFELVADFAPNYTFNSITQRFIKAPYTFTEELKRDALPKTNPVFLFGNKYLNAAY
ANSIELYKNFIGVPHIQSILRIVGKKNLPLIVAEVLRNIEIKITNVLSPYVSELLQGMPA
STKLPIYDYGTE---

---fnp*ycss*lvwcssr*wchlr*flnrvlsfmecpsicnllatlk*k**ifp*ivw*
wfnvgrlfidpfpwstisfrii*fllshfkcrrgcccsfr*tclkefl*ecsnhqryq*s
nl*ytqyilsttttf*ydpfttcy*ts*tiysynhc*rtinstttttttttttstinfkf
wk*fitfccwcttsttttinkwfttstt

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB604 (AFB604Q) /CSM/AF/AFB6-A/AFB604Q.Seq.d/                       2034   0.0
VHP502 (VHP502Q) /CSM/VH/VHP5-A/VHP502Q.Seq.d/                        924   0.0
VHJ827 (VHJ827Q) /CSM/VH/VHJ8-B/VHJ827Q.Seq.d/                        924   0.0
AFH509 (AFH509Q) /CSM/AF/AFH5-A/AFH509Q.Seq.d/                        924   0.0
VHE283 (VHE283Q) /CSM/VH/VHE2-D/VHE283Q.Seq.d/                        918   0.0
AHJ856 (AHJ856Q) /CSM/AH/AHJ8-C/AHJ856Q.Seq.d/                        918   0.0
AHK356 (AHK356Q) /CSM/AH/AHK3-C/AHK356Q.Seq.d/                        880   0.0
FC-AD16 (FC-AD16Q) /CSM/FC/FC-AD/FC-AD16Q.Seq.d/                      860   0.0
AHI551 (AHI551Q) /CSM/AH/AHI5-C/AHI551Q.Seq.d/                        821   0.0
AHG569 (AHG569Q) /CSM/AH/AHG5-C/AHG569Q.Seq.d/                        670   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BI512543|BI512543.1 BB160009B10C07.5 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160009B10C07 5', mRNA sequence.    48  0.25  1
AC117678|AC117678.3 Mus musculus clone RP23-421M9, WORKING DRAFT SEQUENCE, 5 unordered pieces.    46  1.0  1
AC066603|AC066603.3 Homo sapiens chromosome 3 clone RP11-690C8 map 3p, WORKING DRAFT SEQUENCE, 21 unordered pieces.    46  1.0  1
BZ979271|BZ979271.1 PUGHP68TD ZM_0.6_1.0_KB Zea mays genomic clone ZMMBTa386K16, DNA sequence.    36  3.7  3
AC123643|AC123643.6 Mus musculus clone RP23-79I18, WORKING DRAFT SEQUENCE, 12 unordered pieces.    44  4.0  1
BY234753|BY234753.1 Mus musculus adult inner ear cDNA, RIKEN full-length enriched library, clone:F930037B06, 5' end partial sequence.    44  4.0  1
CD758457|CD758457.1 AGENCOURT_14622411 NCI_CGAP_ZKid1 Danio rerio cDNA clone IMAGE:6960337 5', mRNA sequence.    44  4.0  1
BG867200|BG867200.1 602786221F1 NCI_CGAP_SG2 Mus musculus cDNA clone IMAGE:4912228 5', mRNA sequence.    44  4.0  1
AC131782|AC131782.2 Mus musculus chromosome UNK clone RP24-361A10, WORKING DRAFT SEQUENCE, 2 unordered pieces.    42  4.3  2
BQ869564|BQ869564.1 QGD6I15.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGD6I15, mRNA sequence.    36  5.5  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q6UK63) RecName: Full=Protein pirA;  &AY368270_1(AY368270|pid:n...   383   e-105
(Q90YM8) RecName: Full=Cytoplasmic FMR1-interacting protein 1 ho...   164   7e-39
EF531617_1(EF531617|pid:none) Danio rerio cytoplasmic FMR1 inter...   161   5e-38
BC163239_1(BC163239|pid:none) Danio rerio zgc:194528, mRNA (cDNA...   161   5e-38
(Q5R414) RecName: Full=Cytoplasmic FMR1-interacting protein 2;  ...   160   8e-38
AM393359_1(AM393359|pid:none) Synthetic construct Homo sapiens c...   159   2e-37
(Q7L576) RecName: Full=Cytoplasmic FMR1-interacting protein 1; A...   159   2e-37
AK147632_1(AK147632|pid:none) Mus musculus adult male brain UNDE...   159   2e-37
AK146613_1(AK146613|pid:none) Mus musculus 17 days pregnant adul...   159   2e-37
AB093288_1(AB093288|pid:none) Mus musculus mRNA for mKIAA1168 pr...   159   2e-37

 psg: 0.75  gvh: 0.33  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial

>> prediction for AFB604 is cyt

>AFB604F.Seq
TAGATTATGCCATCAGTAGATTCGAAGCTTGCGATTTAACATCGATCGTTGAATTGGAGA
CCCAAATCGCCAATATCAAACTCACTCACAAACTCTTATCAGAATACTTTGATATAGATC
CATTCGAATCAATCTTTAATGAAATCAATGAGTCCACTTCTTTGGTCTCTTATCATGGTC
GTATTGTACTTCATATTATCTTTGAATTGGTGGCCGATTTCGCTCCAAACTATACCTTCA
ATTCAATCACTCAACGTTTTATAAAGGCTCCATACACTTTCACCGAAGAATTGAAACGTG
ATGCACTTCCAAAAACTAATCCTGTCTTTTTGTTTGGTAATAAATATTTGAATGCTGCCT
ATGCAAACTCGATCGAACTCTACAAGAATTTCATTGGTGTACCACATATTCAATCAATCC
TTAGAATCGTTGGTAAAAAGAATCTTCCTTTGATCGTTGCTGAGGTATTACGTAATATTG
AAATCAAGATCACCAATGTTCTCTCACCATACGTTAGTGAATTACTTCAAGGTATGCCTG
CATCGACTAAATTACCAATCTATGATTATGGTACTGAAGG----------

>AFB604Z.Seq
----------TCTTCAATCCTTAATATTGTTCGTCATGATTGGTCTGGTGCTCCTCCAGA
TAATGGTGTCATTTGCGTTGATTCCTCAACAGAGTTTTATCGTTTATGGAGTGCCCTTCA
ATTTGTAATCTGTTGGCCACTCTCAAATGAAAATGATAAATCTTTCCATGAATTGTTTGG
TGATGGTTTAATGTGGGCCGGTTGTTCATTGATCCATTTCCTTGGTCAACAATATCGTTT
CGAATTATTTGATTTCTGTTATCACATTTTAAATGTAGAAGAGGCTGCTGCTGTTCGTTC
AGATAAACCTGCCTTAAAGAATTTCTTTAAGAATGCTCAAATCATCAAAGATATCAATAA
TCAAATCTTTAGTATACTCAATACATATTGTCCACCACCACCACCTTCTAATATGATCCT
TTCACCACCTGCTACTGAACAAGCTGAACAATTTATAGTTACAACCACTGTTGAAGAACA
ATCAATTCAACAACAACAACAACAACAACAACAACAACAACTTCAACAATCAACTTCAAA
TTCTGGAAGTAGTTTATCACTTTCTGCTGCTGGTGCACCACCTCCACCACCACCACCATC
AACAAATGGTTTACCACCTCCACCACCAA

>AFB604P.Seq
TAGATTATGCCATCAGTAGATTCGAAGCTTGCGATTTAACATCGATCGTTGAATTGGAGA
CCCAAATCGCCAATATCAAACTCACTCACAAACTCTTATCAGAATACTTTGATATAGATC
CATTCGAATCAATCTTTAATGAAATCAATGAGTCCACTTCTTTGGTCTCTTATCATGGTC
GTATTGTACTTCATATTATCTTTGAATTGGTGGCCGATTTCGCTCCAAACTATACCTTCA
ATTCAATCACTCAACGTTTTATAAAGGCTCCATACACTTTCACCGAAGAATTGAAACGTG
ATGCACTTCCAAAAACTAATCCTGTCTTTTTGTTTGGTAATAAATATTTGAATGCTGCCT
ATGCAAACTCGATCGAACTCTACAAGAATTTCATTGGTGTACCACATATTCAATCAATCC
TTAGAATCGTTGGTAAAAAGAATCTTCCTTTGATCGTTGCTGAGGTATTACGTAATATTG
AAATCAAGATCACCAATGTTCTCTCACCATACGTTAGTGAATTACTTCAAGGTATGCCTG
CATCGACTAAATTACCAATCTATGATTATGGTACTGAAGG----------TCTTCAATCC
TTAATATTGTTCGTCATGATTGGTCTGGTGCTCCTCCAGATAATGGTGTCATTTGCGTTG
ATTCCTCAACAGAGTTTTATCGTTTATGGAGTGCCCTTCAATTTGTAATCTGTTGGCCAC
TCTCAAATGAAAATGATAAATCTTTCCATGAATTGTTTGGTGATGGTTTAATGTGGGCCG
GTTGTTCATTGATCCATTTCCTTGGTCAACAATATCGTTTCGAATTATTTGATTTCTGTT
ATCACATTTTAAATGTAGAAGAGGCTGCTGCTGTTCGTTCAGATAAACCTGCCTTAAAGA
ATTTCTTTAAGAATGCTCAAATCATCAAAGATATCAATAATCAAATCTTTAGTATACTCA
ATACATATTGTCCACCACCACCACCTTCTAATATGATCCTTTCACCACCTGCTACTGAAC
AAGCTGAACAATTTATAGTTACAACCACTGTTGAAGAACAATCAATTCAACAACAACAAC
AACAACAACAACAACAACAACTTCAACAATCAACTTCAAATTCTGGAAGTAGTTTATCAC
TTTCTGCTGCTGGTGCACCACCTCCACCACCACCACCATCAACAAATGGTTTACCACCTC
CACCACCAA