>VHC494 (VHC494Q) /CSM/VH/VHC4-D/VHC494Q.Seq.d/
XXXXXXXXXXATACCAAAATAAATATTGGATTTATAAATATCAGCATATATACGCCCCAT
TTTTGTATGGTCTATATTCGATTAAATATAGATTATGTGATTATAGTGTTTTCACAGAGG
GTTCAATTGGAAGGGTTAGAACTGCAAATGCTTCAAATTTTGAAATCATCTCTTTTATCA
TTGGTAAATTGGTTTTCATTGTTTTTAGATTCATCATACCATTACAATATCATAGTTTAG
TAAACTTGTTAACTTATTTCTTTATCGCTGAATTCTTTTTTGGTCTTTATTTATCCTTTG
GTTTCCAAGTTAGTCATTCTGCTGATAATTTAAAAATTGTTGCAACAAGTGTAAATGAAA
ATGATGAACCAACAAATGTTGATGAAGGTTGGGCAATTCATCAAATTAAAACTACTCAAG
ATTATGGTATTAATTCATATATGTGTTTATTTTTTTCGGGTGGTGTAAATTTACAAGTTG
TTCATCACTTGTTTCCATCAATTTCACAAGGATACTATGGTGAATTAGTACCAATTATTA
AAAAGGTTTGTGATGAGTATGATGTACATTATAATATTCAACCAACATTTTATGCTGCTT
TTAAATCTCATATTGATTTTCTTTACAATATGGGTAATAATGAAAATTATGTAAGAAAAT
CTGTT

---YQNKYWIYKYQHIYAPFLYGLYSIKYRLCDYSVFTEGSIGRVRTANASNFEIISFII
GKLVFIVFRFIIPLQYHSLVNLLTYFFIAEFFFGLYLSFGFQVSHSADNLKIVATSVNEN
DEPTNVDEGWAIHQIKTTQDYGINSYMCLFFSGGVNLQVVHHLFPSISQGYYGELVPIIK
KVCDEYDVHYNIQPTFYAAFKSHIDFLYNMGNNENYVRKSV

Frame A:
---YQNKYWIYKYQHIYAPFLYGLYSIKYRLCDYSVFTEGSIGRVRTANASNFEIISFII
GKLVFIVFRFIIPLQYHSLVNLLTYFFIAEFFFGLYLSFGFQVSHSADNLKIVATSVNEN
DEPTNVDEGWAIHQIKTTQDYGINSYMCLFFSGGVNLQVVHHLFPSISQGYYGELVPIIK
KVCDEYDVHYNIQPTFYAAFKSHIDFLYNMGNNENYVRKSV


Frame B:
---tkinigfinisiytphfcmvyirlnidyviivfsqrvqleglelqmlqilkssllsl
vnwfslfldssyhyniiv**tc*lislslnsflvfiyplvsklvillii*kllqqv*mkm
mnqqmlmkvgqfiklkllkimvlihicvyffrvv*iyklfitcfhqfhkdtmvn*yqllk
rfvmsmmyiiifnqhfmlllnlilifftiwvimkim*enl


Frame C:
---pk*ildl*isayirpifvwsifd*i*im*l*cfhrgfnwkg*nckcfkf*nhlfyhw
*igfhcf*ihhtitis*fsklvnlflyr*ilfwslfilwfps*sfc**fknccnkck*k*
*tnkc**rlgnssn*nysrlwy*fiyvfiffgwckftscsslvsinftrilw*istny*k
gl**v*ctl*ystnilccf*isy*fslqyg***klckkic

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC494 (VHC494Q) /CSM/VH/VHC4-D/VHC494Q.Seq.d/                       1257   0.0
AFK718 (AFK718Q) /CSM/AF/AFK7-A/AFK718Q.Seq.d/                       1209   0.0
CHB310 (CHB310Q) /CSM/CH/CHB3-A/CHB310Q.Seq.d/                       1072   0.0
VFM167 (VFM167Q) /CSM/VF/VFM1-C/VFM167Q.Seq.d/                        686   0.0
SFJ777 (SFJ777Q) /CSM/SF/SFJ7-D/SFJ777Q.Seq.d/                        521   e-147
VSH813 (VSH813Q) /CSM/VS/VSH8-A/VSH813Q.Seq.d/                         62   1e-08
VSF803 (VSF803Q) /CSM/VS/VSF8-A/VSF803Q.Seq.d/                         62   1e-08
VSF218 (VSF218Q) /CSM/VS/VSF2-A/VSF218Q.Seq.d/                         62   1e-08
VSC833 (VSC833Q) /CSM/VS/VSC8-B/VSC833Q.Seq.d/                         62   1e-08
VFO589 (VFO589Q) /CSM/VF/VFO5-D/VFO589Q.Seq.d/                         62   1e-08

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AB022097|AB022097.1 Dictyostelium discoideum FadB mRNA for delta 5 fatty acid desaturase, complete cds.    60   5e-15  3
CS204284|CS204284.1 Sequence 45 from Patent EP1598422.             62   2e-14  4
BD092935|BD092935.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants.    62   2e-14  4
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.    62   2e-14  4
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.    62   2e-14  4
AB029311|AB029311.1 Dictyostelium discoideum Dd des5 gene for fatty acid desaturase, complete cds.    62   4e-11  4
AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          34   0.12   14
EK356568|EK356568.1 1095468560903 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005861985 5', genomic survey sequence.    44   0.19   2
AM485590|AM485590.1 Vitis vinifera, whole genome shotgun sequence, contig  VV78X126751.10, clone ENTAV 115.    46   0.30   2
DY364254|DY364254.1 ZO__Ee0002H03.r ZO__Ee Zingiber officinale cDNA clone ZO__Ee0002H03 3', mRNA sequence.    48   0.32   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   449   e-125
(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   257   2e-67
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   239   4e-62
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   137   4e-31
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   137   4e-31
CQ831428_1(CQ831428|pid:none) Sequence 9 from Patent WO2004057001.    134   2e-30
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   134   2e-30
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   130   3e-29
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   129   1e-28
CP001574_328(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   124   2e-27

 psg: 0.89  gvh: 0.51  alm: 0.23  top: 0.63  tms: 0.07  mit: 0.30  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VHC494 is cyt

>VHC494Z.Seq
NNNNNNNNNNATACCAAAATAAATATTGGATTTATAAATATCAGCATATATACGCCCCAT
TTTTGTATGGTCTATATTCGATTAAATATAGATTATGTGATTATAGTGTTTTCACAGAGG
GTTCAATTGGAAGGGTTAGAACTGCAAATGCTTCAAATTTTGAAATCATCTCTTTTATCA
TTGGTAAATTGGTTTTCATTGTTTTTAGATTCATCATACCATTACAATATCATAGTTTAG
TAAACTTGTTAACTTATTTCTTTATCGCTGAATTCTTTTTTGGTCTTTATTTATCCTTTG
GTTTCCAAGTTAGTCATTCTGCTGATAATTTAAAAATTGTTGCAACAAGTGTAAATGAAA
ATGATGAACCAACAAATGTTGATGAAGGTTGGGCAATTCATCAAATTAAAACTACTCAAG
ATTATGGTATTAATTCATATATGTGTTTATTTTTTTCGGGTGGTGTAAATTTACAAGTTG
TTCATCACTTGTTTCCATCAATTTCACAAGGATACTATGGTGAATTAGTACCAATTATTA
AAAAGGTTTGTGATGAGTATGATGTACATTATAATATTCAACCAACATTTTATGCTGCTT
TTAAATCTCATATTGATTTTCTTTACAATATGGGTAATAATGAAAATTATGTAAGAAAAT
CTGTT