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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHC467
Library VH
(Link to library)
Clone ID VHC467
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHC467P
(Link to Original site)
Representative DNA sequence 
>VHC467 (VHC467Q) /CSM/VH/VHC4-C/VHC467Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGNGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCANATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCACGCT
GGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AGTTXXXXXXXXXXCGGTTCCCCATTATTATCTGTCAAAGCTCATTTTCCAGTATTAGAA
TCCTTCGGTTTCACCGGTGATTTACGTTCCCNCAGTGCCGGTCAAGNTTTCCCNCAATGT
GTCTTNNATCATTGGGCTTCAATTGGTGTTGTCATACAAAGATAAGAAAGCCANTGAAGT
CGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATT
CCACGAAAAACTCTAAACAATNTAAGTCGCACT
sequence update 2002.10.25
Translated Amino Acid sequence 
nsrvqkk*stkwlisqsikfvplwtvxkiseicllllmsitvkplyxil*skelvllpik
flvi*DICHVVLMNKSVVSPLNLHLYHFTSKCQRKTNYHAGCTSHEFLINLIDSPGHVDF
S---

---GSPLLSVKAHFPVLESFGFTGDLRSXSAGQXFPQCVXXHWASIGVVIQR*esx*srf
shqkekrfsprnprs*qiprktlnnxsrt
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvxkiseicllllmsitvkplyxil*skelvllpik
flvi*DICHVVLMNKSVVSPLNLHLYHFTSKCQRKTNYHAGCTSHEFLINLIDSPGHVDF
S---

---rfpiiicqssfssirilrfhr*ftfpqcrsxfpxmclxslgfnwcchtkirkpxksl
*pperekv*pqksqiltnstknskqxksh

Frame B:
tleckkskvqng*fhnrsnschygpsxkyqkyvcycscrsr*nhfixffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqittlvvlhtnf*silsipqvtlis
v---

---GSPLLSVKAHFPVLESFGFTGDLRSXSAGQXFPQCVXXHWASIGVVIQR*esx*srf
shqkekrfsprnprs*qiprktlnnxsrt

Frame C:
lssakkvkykmvnftidqiraimdrrenirnmsviahvdhgkttlsxsliqragiiadkv
sgdmrymscradeqergitiksssvslhfempkedklprwlyftrifnqsyrfprsr*fq
---

---vphyylsklifqy*npsvspviyvpxvpvkxsxnvsxiiglqlvlsykdkkaxeval
atrkrkglapeipdldkfhekl*tx*va
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC467 (VHC467Q) /CSM/VH/VHC4-C/VHC467Q.Seq.d/                       1102   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/             654   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                        654   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                        654   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/             654   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                        654   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                        654   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                        654   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                        654   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                        654   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   533   0.0    4
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    72   9e-23  4
Y09664|Y09664.1 C.albicans EFT2 gene.                              72   5e-22  4
CO080174|CO080174.1 GR__Ea43H12.r GR__Ea Gossypium raimondii cDNA clone GR__Ea43H12 3', mRNA sequence.    58   3e-17  4
EL513430|EL513430.1 CHUX465.b1_A21.ab1 CHU(XYZ) puzzle sunflower Helianthus paradoxus cDNA clone CHUX465, mRNA sequence.    72   1e-16  4
EL476245|EL476245.1 CHUL4181.b1_J14.ab1 CHU(LMS) puzzle sunflower Helianthus paradoxus cDNA clone CHUL4181, mRNA sequence.    72   1e-16  4
DY941362|DY941362.1 CHPX9185.b1_B18.ab1 CHP(XYZ) plains sunflower Helianthus petiolaris cDNA clone CHPX9185, mRNA sequence.    72   1e-16  4
DY938228|DY938228.1 CHPX5032.b1_O09.ab1 CHP(XYZ) plains sunflower Helianthus petiolaris cDNA clone CHPX5032, mRNA sequence.    72   1e-16  4
DY907212|DY907212.1 CHAX1940.b1_G05.ab1 CHA(XYZ) common wild sunflower Helianthus annuus cDNA clone CHAX1940, mRNA sequence.    72   1e-16  4
AR550598|AR550598.1 Sequence 5729 from patent US 6747137.          60   4e-16  3
dna update 2007. 4.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   117   6e-30
A40411(A40411) translation elongation factor eEF-2 - Caenorhabdi...   116   4e-29
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   113   5e-29
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   114   1e-28
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   114   1e-28
AY144924_1(AY144924|pid:none) Saccharomyces castellii clone Cont...   114   1e-28
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   114   2e-28
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   114   2e-28
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   109   2e-28
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   113   2e-28
protein update 2009. 6.28
PSORT 

 psg: 0.83  gvh: 0.40  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: endoplasmic reticulum

>> prediction for VHC467 is cyt
5' end seq. ID VHC467F
5' end seq. 
>VHC467F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGNGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCANATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCACGCT
GGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AGTTNNNNNNNNNN
Length of 5' end seq. 374
3' end seq. ID VHC467Z
3' end seq. 
>VHC467Z.Seq
NNNNNNNNNNCGGTTCCCCATTATTATCTGTCAAAGCTCATTTTCCAGTATTAGAATCCT
TCGGTTTCACCGGTGATTTACGTTCCCNCAGTGCCGGTCAAGNTTTCCCNCAATGTGTCT
TNNATCATTGGGCTTCAATTGGTGTTGTCATACAAAGATAAGAAAGCCANTGAAGTCGCT
TTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCAC
GAAAAACTCTAAACAATNTAAGTCGCACT
Length of 3' end seq. 269
Connected seq. ID VHC467P
Connected seq. 
>VHC467P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGNGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCANATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCACGCT
GGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AGTT----------CGGTTCCCCATTATTATCTGTCAAAGCTCATTTTCCAGTATTAGAA
TCCTTCGGTTTCACCGGTGATTTACGTTCCCNCAGTGCCGGTCAAGNTTTCCCNCAATGT
GTCTTNNATCATTGGGCTTCAATTGGTGTTGTCATACAAAGATAAGAAAGCCANTGAAGT
CGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATT
CCACGAAAAACTCTAAACAATNTAAGTCGCACT
Length of connected seq. 623
Full length Seq ID -
Full length Seq. -
Length of full length seq. -