>VHC329 (VHC329Q) /CSM/VH/VHC3-B/VHC329Q.Seq.d/
CTTAATTTTTCCTCCCAATCATACTTACATATAAATATAAATATATATATATATACAAAT
AAATCATGGCATCTATTATTCGTAATAGAAACCTTATAAAATCATATGAAAAAATAAAAA
AACCAATTACAATAACACAAACTTCATTATTTTCAAAACAACAATATAGTAATAATATTA
AATGTAACTTTTCAAGTAATAATAATCTATTTAAAAATCAAAATGAAAATGAAAATCAAA
CAAGTATTTTAAATAGATTTTTTAATAATGAATATATAACATCACCTTCATTCAGTATAA
AGTCAATAAAAGATTCAATTTTTAAATCAGAAGGATGGGGTAATTTCGAAAAAAAAGAAA
AAGAAACTGGTAACGACAACAACAACAACAAAGGCAACAATAATAATAAAGATAATAAAG
ATAATAAAGATAATAAAGATAATAAAGATAATAAAGATAATAAAGATGAAAGTTTTATGC
AACAAATGAATTCACCAGAATTTAAGAAATATTTAATTACAGTTATATCAATXXXXXXXX
XXACAGTTGCATATCATGAAGCTGGTCATGCCGTCGTAAGTTGGTTCCTCAAACATTGTC
ATCCACTATTGAAAGTTTCAATTGTACCAAGAGGTGTTGCCGCTCTAGGTTATGCTCAAT
ATCTTCCAAAGGAGCAATTCCTTCAAAATCAAGAACAAATCTTTGATATGATGTGTATGG
CTTTAGGTGGTCGTGTAGCTGAACAATTAACCTTTGGTACCATTACAACTGGTGCTCAAG
ATGATCTTGAGAAAATCACTAAAATGGCTTACTCTCAAGTCTCAATCTATGGTATGAATG
AAAAAATAGGTCCATTATCCTATCAAAAAGGTCAAGATGGTTCTGATTTAACCAAACCTT
ACAGTGAAGAAACTGCTGAAGTCATGGATGAAGAGGTTAGAAAATTACTTAAATCCGCTT
ATGATCGTACCACTCAAGTCTTACAAGAACATAGAGAAGGTTTAATTTCTGTAGCTAATT
TATTACTTGAAAAAGAAGTTATTCACTTTGAAGAAGTTGAAGCTGTCTTAGGTCCACGTC
CTTTTAATAATAAAACTGAAGAACCAAAAAATATTAATAATCAACCAAAAGAATCAATTA
ATGCTTCTTCATAAAAAATAAATAAATAAATAAATAAATAATAATAAATGGTAATCAAAA
ATAAAATAATTATTATAATAATTATAATAACATTACGC

*fflpiiltykykyiyiyk*IMASIIRNRNLIKSYEKIKKPITITQTSLFSKQQYSNNIK
CNFSSNNNLFKNQNENENQTSILNRFFNNEYITSPSFSIKSIKDSIFKSEGWGNFEKKEK
ETGNDNNNNKGNNNNKDNKDNKDNKDNKDNKDNKDESFMQQMNSPEFKKYLITVIS---

---TVAYHEAGHAVVSWFLKHCHPLLKVSIVPRGVAALGYAQYLPKEQFLQNQEQIFDMM
CMALGGRVAEQLTFGTITTGAQDDLEKITKMAYSQVSIYGMNEKIGPLSYQKGQDGSDLT
KPYSEETAEVMDEEVRKLLKSAYDRTTQVLQEHREGLISVANLLLEKEVIHFEEVEAVLG
PRPFNNKTEEPKNINNQPKESINASS*kink*ink***mviknkiiiiiiiitlr

Frame A:
lnfssqsylhininiyiytnkswhllfvietl*nhmkk*knqlq*hklhyfqnnniviil
nvtfqviiiylkikmkmkikqvf*idflimni*hhlhsv*sq*kiqflnqkdgviskkkk
kklvttttttkatiiikiikiikiikiikiikiikmkvlcnk*ihqnlrni*lqlyq---

---TVAYHEAGHAVVSWFLKHCHPLLKVSIVPRGVAALGYAQYLPKEQFLQNQEQIFDMM
CMALGGRVAEQLTFGTITTGAQDDLEKITKMAYSQVSIYGMNEKIGPLSYQKGQDGSDLT
KPYSEETAEVMDEEVRKLLKSAYDRTTQVLQEHREGLISVANLLLEKEVIHFEEVEAVLG
PRPFNNKTEEPKNINNQPKESINASS*kink*ink***mviknkiiiiiiiitlr

Frame B:
lifppnhtyi*i*iyiyiqinhgiyys**kpykii*knkktnynntnfiifktti***y*
m*lfk***si*ksk*k*ksnkyfk*if***iynitfiqykvnkrfnf*irrmg*frkkrk
rnw*rqqqqqrqq***r**r**r**r**r**r**r*kfyatneftri*eifnysyin---

---qlhimklvmps*vgssnivihy*kfqlyqevlpl*vmlnifqrsnsfkiknksli*c
vwl*vvv*lnn*plvplqlvlkmilrkslkwltlksqsmv*mkk*vhypikkvkmvli*p
nltvkkllkswmkrlenylnplmivplksykniekv*fl*liyylkkklftlkklkls*v
hvlliiklknqkiliinqknqlmllhkk*ink*innnkw*skik*ll**l**hy

Frame C:
*fflpiiltykykyiyiyk*IMASIIRNRNLIKSYEKIKKPITITQTSLFSKQQYSNNIK
CNFSSNNNLFKNQNENENQTSILNRFFNNEYITSPSFSIKSIKDSIFKSEGWGNFEKKEK
ETGNDNNNNKGNNNNKDNKDNKDNKDNKDNKDNKDESFMQQMNSPEFKKYLITVIS---

---scis*swscrrklvpqtlsstiesfnctkrccrsrlcsisskgaipsksrtnl*ydv
ygfrwscs*tinlwyhynwcsr*s*enh*ngllsslnlwye*knrsiilskrsrwf*fnq
tlq*rnc*shg*rg*kit*irl*syhssltrt*rrfnfcs*fit*krsysl*rs*sclrs
tsf***n*rtkky**stkrin*cffiknk*ink*iiingnqk*nnyynnynnit

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC329 (VHC329Q) /CSM/VH/VHC3-B/VHC329Q.Seq.d/                       1294   0.0
VHO815 (VHO815Q) /CSM/VH/VHO8-A/VHO815Q.Seq.d/                       1205   0.0
VHO808 (VHO808Q) /CSM/VH/VHO8-A/VHO808Q.Seq.d/                       1205   0.0
VHJ739 (VHJ739Q) /CSM/VH/VHJ7-B/VHJ739Q.Seq.d/                       1205   0.0
VHA666 (VHA666Q) /CSM/VH/VHA6-C/VHA666Q.Seq.d/                       1205   0.0
FC-AN10 (FC-AN10Q) /CSM/FC/FC-AN/FC-AN10Q.Seq.d/                     1205   0.0
VHJ184 (VHJ184Q) /CSM/VH/VHJ1-D/VHJ184Q.Seq.d/                       1189   0.0
VHA435 (VHA435Q) /CSM/VH/VHA4-B/VHA435Q.Seq.d/                       1150   0.0
AHD683 (AHD683Q) /CSM/AH/AHD6-D/AHD683Q.Seq.d/                       1150   0.0
CHJ235 (CHJ235Q) /CSM/CH/CHJ2-B/CHJ235Q.Seq.d/                        587   e-166

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CV368516|CV368516.1 PM2-ET0256-010301-003-c06 ET0256 Homo sapiens cDNA, mRNA sequence.    46   8e-12  4
BM699661|BM699661.1 UI-E-DW1-agz-e-13-0-UI.r1 UI-E-DW1 Homo sapiens cDNA clone UI-E-DW1-agz-e-13-0-UI 5', mRNA sequence.    46   2e-11  4
BM762949|BM762949.1 K-EST0044178 S13KMS5 Homo sapiens cDNA clone S13KMS5-13-F10 5', mRNA sequence.    46   2e-11  4
CV573985|CV573985.1 oe18g06.y1 Human keratoconus cornea, unamplified, (od/oe) Homo sapiens cDNA clone oe18g06 5', mRNA sequence.    46   2e-11  4
AP005264|AP005264.3 Homo sapiens genomic DNA, chromosome 18 clone:RP11-64C12, complete sequence.    46   2e-11  3
BM042033|BM042033.1 603616029F1 NIH_MGC_112 Homo sapiens cDNA clone IMAGE:5420646 5', mRNA sequence.    46   2e-11  4
AC198527|AC198527.1 Macaca mulatta chromosome UNK clone CH250-29L19, WORKING DRAFT SEQUENCE, 15 unordered pieces.    46   2e-11  3
BQ635933|BQ635933.1 hd02b10.y1 Human Retina cDNA (Un-normalized, unamplified): hd/he Homo sapiens cDNA clone hd02b10 5', mRNA sequence.    46   2e-11  4
CN425721|CN425721.1 17000531610369 GRN_EB Homo sapiens cDNA 5', mRNA sequence.    46   2e-11  4
BG474275|BG474275.1 602516925F1 NIH_MGC_16 Homo sapiens cDNA clone IMAGE:4648590 5', mRNA sequence.    46   2e-11  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q0DHL4) RecName: Full=Cell division protease ftsH homolog 8, mi...   234   6e-60
AC135467_32(AC135467|pid:none) Medicago truncatula clone mth2-34...   234   6e-60
AF397903_1(AF397903|pid:none) Pisum sativum AAA-metalloprotease ...   233   1e-59
(Q8S2A7) RecName: Full=Cell division protease ftsH homolog 3, mi...   232   2e-59
AC005315_14(AC005315|pid:none) Arabidopsis thaliana chromosome 2...   229   1e-58
AK226271_1(AK226271|pid:none) Arabidopsis thaliana mRNA for AAA-...   229   1e-58
FB781832_1(FB781832|pid:none) Sequence 1105 from Patent WO200803...   229   1e-58
(Q84WU8) RecName: Full=Cell division protease ftsH homolog 3, mi...   229   1e-58
AY063914_1(AY063914|pid:none) Arabidopsis thaliana putative AAA-...   228   4e-58
BT069905_1(BT069905|pid:none) Zea mays full-length cDNA clone ZM...   227   6e-58

 psg: 0.50  gvh: 0.34  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.42  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: mitochondrial
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for VHC329 is nuc

>VHC329F.Seq
CTTAATTTTTCCTCCCAATCATACTTACATATAAATATAAATATATATATATATACAAAT
AAATCATGGCATCTATTATTCGTAATAGAAACCTTATAAAATCATATGAAAAAATAAAAA
AACCAATTACAATAACACAAACTTCATTATTTTCAAAACAACAATATAGTAATAATATTA
AATGTAACTTTTCAAGTAATAATAATCTATTTAAAAATCAAAATGAAAATGAAAATCAAA
CAAGTATTTTAAATAGATTTTTTAATAATGAATATATAACATCACCTTCATTCAGTATAA
AGTCAATAAAAGATTCAATTTTTAAATCAGAAGGATGGGGTAATTTCGAAAAAAAAGAAA
AAGAAACTGGTAACGACAACAACAACAACAAAGGCAACAATAATAATAAAGATAATAAAG
ATAATAAAGATAATAAAGATAATAAAGATAATAAAGATAATAAAGATGAAAGTTTTATGC
AACAAATGAATTCACCAGAATTTAAGAAATATTTAATTACAGTTATATCAATNNNNNNNN
NN

>VHC329Z.Seq
NNNNNNNNNNACAGTTGCATATCATGAAGCTGGTCATGCCGTCGTAAGTTGGTTCCTCAA
ACATTGTCATCCACTATTGAAAGTTTCAATTGTACCAAGAGGTGTTGCCGCTCTAGGTTA
TGCTCAATATCTTCCAAAGGAGCAATTCCTTCAAAATCAAGAACAAATCTTTGATATGAT
GTGTATGGCTTTAGGTGGTCGTGTAGCTGAACAATTAACCTTTGGTACCATTACAACTGG
TGCTCAAGATGATCTTGAGAAAATCACTAAAATGGCTTACTCTCAAGTCTCAATCTATGG
TATGAATGAAAAAATAGGTCCATTATCCTATCAAAAAGGTCAAGATGGTTCTGATTTAAC
CAAACCTTACAGTGAAGAAACTGCTGAAGTCATGGATGAAGAGGTTAGAAAATTACTTAA
ATCCGCTTATGATCGTACCACTCAAGTCTTACAAGAACATAGAGAAGGTTTAATTTCTGT
AGCTAATTTATTACTTGAAAAAGAAGTTATTCACTTTGAAGAAGTTGAAGCTGTCTTAGG
TCCACGTCCTTTTAATAATAAAACTGAAGAACCAAAAAATATTAATAATCAACCAAAAGA
ATCAATTAATGCTTCTTCATAAAAAATAAATAAATAAATAAATAAATAATAATAAATGGT
AATCAAAAATAAAATAATTATTATAATAATTATAATAACATTACGC

>VHC329P.Seq
CTTAATTTTTCCTCCCAATCATACTTACATATAAATATAAATATATATATATATACAAAT
AAATCATGGCATCTATTATTCGTAATAGAAACCTTATAAAATCATATGAAAAAATAAAAA
AACCAATTACAATAACACAAACTTCATTATTTTCAAAACAACAATATAGTAATAATATTA
AATGTAACTTTTCAAGTAATAATAATCTATTTAAAAATCAAAATGAAAATGAAAATCAAA
CAAGTATTTTAAATAGATTTTTTAATAATGAATATATAACATCACCTTCATTCAGTATAA
AGTCAATAAAAGATTCAATTTTTAAATCAGAAGGATGGGGTAATTTCGAAAAAAAAGAAA
AAGAAACTGGTAACGACAACAACAACAACAAAGGCAACAATAATAATAAAGATAATAAAG
ATAATAAAGATAATAAAGATAATAAAGATAATAAAGATAATAAAGATGAAAGTTTTATGC
AACAAATGAATTCACCAGAATTTAAGAAATATTTAATTACAGTTATATCAAT--------
--ACAGTTGCATATCATGAAGCTGGTCATGCCGTCGTAAGTTGGTTCCTCAAACATTGTC
ATCCACTATTGAAAGTTTCAATTGTACCAAGAGGTGTTGCCGCTCTAGGTTATGCTCAAT
ATCTTCCAAAGGAGCAATTCCTTCAAAATCAAGAACAAATCTTTGATATGATGTGTATGG
CTTTAGGTGGTCGTGTAGCTGAACAATTAACCTTTGGTACCATTACAACTGGTGCTCAAG
ATGATCTTGAGAAAATCACTAAAATGGCTTACTCTCAAGTCTCAATCTATGGTATGAATG
AAAAAATAGGTCCATTATCCTATCAAAAAGGTCAAGATGGTTCTGATTTAACCAAACCTT
ACAGTGAAGAAACTGCTGAAGTCATGGATGAAGAGGTTAGAAAATTACTTAAATCCGCTT
ATGATCGTACCACTCAAGTCTTACAAGAACATAGAGAAGGTTTAATTTCTGTAGCTAATT
TATTACTTGAAAAAGAAGTTATTCACTTTGAAGAAGTTGAAGCTGTCTTAGGTCCACGTC
CTTTTAATAATAAAACTGAAGAACCAAAAAATATTAATAATCAACCAAAAGAATCAATTA
ATGCTTCTTCATAAAAAATAAATAAATAAATAAATAAATAATAATAAATGGTAATCAAAA
ATAAAATAATTATTATAATAATTATAATAACATTACGC