>VHC307 (VHC307Q) /CSM/VH/VHC3-A/VHC307Q.Seq.d/
ACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTCAAAATGAA
GAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTA
ACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCA
TTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAAT
AAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAAT
AAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCA
GAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAA
GGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTT
GTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAA
TCACAAAAGAATATACATACACAATTCTTTAAGAAXXXXXXXXXXTTTTAGATCATTTTA
GACTTTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCAGATCCGGTG
GTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAAT
TCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAATT
TGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACTTTAAGA
TGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAATT
TCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCAATAGTAAAG
ATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTGATGATG
GCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATTTCGATT
CTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAAAGAGAATGTTGG
CTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTAAAAAGATTC
AAAATTTCCAACAAGAATAGAATTATTTAAATATTCTTTCNCGGTTCAAGA

tnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIEEA
LDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKETA
ESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASIYQ
SQKNIHTQFFK---

---LDHFRLLITHIGNAMGYIRLVRSGGLHYCSNAIKFVPDLKKIPKFQDLTSKDALSPE
TIQASTNLDSIIHNLSNNLSEGTEYFKMLVNVFATEFRNIANQHLKNFYIIVPALASNYI
DHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKLLDQNKDFDSLHWFDRIQLKCE
TDQKRMLAEANAKGIKEDQNHLAIKKIQNFQQE*nylnilsrfk

Frame A:
tnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIEEA
LDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKETA
ESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASIYQ
SQKNIHTQFFK---

---f*iildf*slilvmlwvildwsdpvvytivvmlsnsyqt*krfqnski*pqkmpshl
kqfklvpiwiqsfiifqiiyqrvlntlrcw*tfslqsfeislini*risissylpwpqit
sii*sivkinslrnqkqsvlklyyslmmalqlvwlis*ny*ikikisilfigsivfn*nv
klikrecwlkqmqkvskrikii*qlkrfkisnknrii*iffxgsr

Frame B:
rttqfrkwphpirlimvkmknihlmhlkmvhlnwlesnn*lnlinlqlimhnkfgr*kkh
*mkh*mkygi*i*iqfqfiinlmnn*i*lnwlkliikfsik*y*yslqfviklef*kkqq
kvnfihh*qylerlqvnhqkvmyklklvnyyhl*liyqhl*idvih*leillvnlhrsin
hkriyihnslr---

---frsf*tfnhsyw*cygly*igqirwftll**cyqirtrlekdskiprfnlkrcplt*
nnss*yqfgfnhs*sfk*firgy*il*dvgkrfryrvskyr*stfkeflyhrtclglklh
rsydq**r*tl*eiksnrc*sfiih**wlcnwfglylkiirsk*rfrfsslvrsysiem*
n*skenvg*skckryqrgsksfsn*kdskfptrielfkysfxvq

Frame C:
eqpnlenghilfg**wsk*riyi*ci*rwfi*igwratin*t*sics*lcttnledrrsi
r*nik*smgykyrssfnl**ts*tirfn*ig*n*s*nfq*snisilfnl*sn*nfkrnsr
k*ilftinsiwrdyr*iirr*ctn*sw*iitiyd*fisick*mlfin*kyy*sicidlsi
tkeytytil*e---

---LDHFRLLITHIGNAMGYIRLVRSGGLHYCSNAIKFVPDLKKIPKFQDLTSKDALSPE
TIQASTNLDSIIHNLSNNLSEGTEYFKMLVNVFATEFRNIANQHLKNFYIIVPALASNYI
DHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKLLDQNKDFDSLHWFDRIQLKCE
TDQKRMLAEANAKGIKEDQNHLAIKKIQNFQQE*nylnilsrfk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC307 (VHC307Q) /CSM/VH/VHC3-A/VHC307Q.Seq.d/                       2415   0.0
VHJ880 (VHJ880Q) /CSM/VH/VHJ8-D/VHJ880Q.Seq.d/                       2391   0.0
VHK424 (VHK424Q) /CSM/VH/VHK4-A/VHK424Q.Seq.d/                       1314   0.0
VHI654 (VHI654Q) /CSM/VH/VHI6-C/VHI654Q.Seq.d/                       1314   0.0
VHA684 (VHA684Q) /CSM/VH/VHA6-D/VHA684Q.Seq.d/                       1314   0.0
SHK609 (SHK609Q) /CSM/SH/SHK6-A/SHK609Q.Seq.d/                       1314   0.0
CHI453 (CHI453Q) /CSM/CH/CHI4-C/CHI453Q.Seq.d/                       1314   0.0
CHG805 (CHG805Q) /CSM/CH/CHG8-A/CHG805Q.Seq.d/                       1314   0.0
CHG421 (CHG421Q) /CSM/CH/CHG4-A/CHG421Q.Seq.d/                       1314   0.0
AHA326 (AHA326Q) /CSM/AH/AHA3-B/AHA326Q.Seq.d/                       1314   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU746157|BU746157.1 CH3#002_C07T7 Canine heart normalized cDNA Library in pBluescript Canis familiaris cDNA clone CH3#002_C07 5', mRNA sequence.    38   7e-04  3
CP000263|CP000263.1 Buchnera aphidicola str. Cc (Cinara cedri), complete genome.    36   0.012  14
BY464171|BY464171.1 Mus musculus 15 days pregnant adult female amnion cDNA, RIKEN full-length enriched library, clone:K630150M24, 3' end partial sequence.    42   0.032  2
AE017308|AE017308.1 Mycoplasma mobile 163K complete genome.        34   0.089  19
BB822673|BB822673.1 Mus musculus mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830022O04, 3' end partial sequence.    32   0.099  4
DN287505|DN287505.1 1241755 MARC 7BOV Bos taurus cDNA 3', mRNA sequence.    34   0.12   3
CR103667|CR103667.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP142o16.    50   0.15   1
CR044130|CR044130.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP186g09.    50   0.15   1
CR034434|CR034434.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP47e19.    50   0.15   1
CR023465|CR023465.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP421e10.    50   0.15   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54R74) RecName: Full=UPF0681 protein;                               421   e-116
AB028956_1(AB028956|pid:none) Homo sapiens mRNA for KIAA1033 pro...   216   1e-54
BC143373_1(BC143373|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   216   1e-54
BC040936_1(BC040936|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   216   1e-54
BC020314_1(BC020314|pid:none) Mus musculus RIKEN cDNA A230046K03...   214   5e-54
(Q3UMB9) RecName: Full=UPF0681 protein KIAA1033;                      214   5e-54
AK161247_1(AK161247|pid:none) Mus musculus adult male testis cDN...   214   5e-54
AK145000_1(AK145000|pid:none) Mus musculus mammary gland RCB-052...   214   5e-54
BC110850_1(BC110850|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   161   5e-38
AX882617_1(AX882617|pid:none) Sequence 17522 from Patent EP10746...   156   1e-36

 psg: 0.79  gvh: 0.25  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHC307 is nuc

>VHC307F.Seq
ACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTCAAAATGAA
GAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTA
ACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCA
TTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAAT
AAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAAT
AAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCA
GAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAA
GGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTT
GTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAA
TCACAAAAGAATATACATACACAATTCTTTAAGAANNNNNNNNNN

>VHC307Z.Seq
NNNNNNNNNNTTTTAGATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGT
TATATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTA
CCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCT
GAAACAATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTA
TCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAAT
ATCGCTAATCAACATTTAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTAC
ATCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCT
GAAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTA
TTAGATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGT
GAAACTGATCAAAAGAGAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAA
AATCATTTAGCAATTAAAAAGATTCAAAATTTCCAACAAGAATAGAATTATTTAAATATT
CTTTCNCGGTTCAAGA

>VHC307P.Seq
ACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTCAAAATGAA
GAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTA
ACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCA
TTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAAT
AAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAAT
AAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCA
GAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAA
GGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTT
GTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAA
TCACAAAAGAATATACATACACAATTCTTTAAGAA----------TTTTAGATCATTTTA
GACTTTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCAGATCCGGTG
GTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAAT
TCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAATT
TGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACTTTAAGA
TGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAATT
TCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCAATAGTAAAG
ATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTGATGATG
GCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATTTCGATT
CTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAAAGAGAATGTTGG
CTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTAAAAAGATTC
AAAATTTCCAACAAGAATAGAATTATTTAAATATTCTTTCNCGGTTCAAGA