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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFN538
Library AF
(Link to library)
Clone ID AFN538
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID AFN538E
(Link to Original site)
Representative DNA sequence 
>AFN538 (AFN538Q) /CSM/AF/AFN5-B/AFN538Q.Seq.d/
AATTATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATA
AAAAAAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTC
AGGTATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAAT
TGTTGGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGT
CGGNGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGG
TATTGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACT
TCGNGTTGCTCCANAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGC
AAATAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGT
TGCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGA
TTCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACCACATG
CTATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAA
CTGAACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATTAAAG
AGAAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCATCAT
CTGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATGAAC
GTTTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTGGTA
TTCATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTAT
ATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGA
ATAAAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCAC
sequence update 2001. 6. 9
Translated Amino Acid sequence 
llnnskdfnyililnk*tnkkknkkwmxkmfkl*llimvqvcvklvllvmmlhvlfshql
lvvqdtlvlwlvwvkrihmsxmklnqkevf*hlsiqlnmvlspigmiwkksgiilfimnf
xllxrniqfx*lklh*iqkqierk*LKSCLKLSTLQQCMLPFKLYYHSMPLVVPLVSLWI
QVMVFPILYQFTKGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKE
KLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGI
HETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAP
Translated Amino Acid sequence (All Frames) 
Frame A:
ny*ttqkiliif*y*inkqikkkikngwxrcssfsy**wfryv*swfcw**cstccfpin
cwsskthwcygwygskgficrx*ssikkryfnt*vsn*twychqlg*ygknlasyfl**t
sxcsxgtsssxn*ssiksksk*rendsnhv*nfqhssnvcchssciitlclwsyhwyryg
fr*wcfpyctnlrrvmhyhmlfyv*i*lvvillit**ks*lnvvthsqqllkeksleilk
rn*htlhwilklkcklqphhlh*rnhmnyqmvkllqlvmnvsvvqkhfsnhhswvwsllv
fmkqpiiqs*nvmlifvkiymvmlyyqvvqlcsqvlliv*ikn*lhlhhqq*klkllhh


Frame B:
iikqlkrf*lyfnik*ink*kkk*kmdgedvqalvidngsgmckagfagddapravfpsi
vgrprhtgvmvgmgqkdsyvgdeaqskrgiltlkypiehgivtnwddmekiwhhtfynel
rvapxehpvllteaplnpkanrekmtqimfetfntpamyvaiqavlslyasgrttgivmd
sgdgvshtvpiyeglcittcyftfrfsws*sy*lhdenln*twllihnnc*krnr*ry*r
eisircigf*s*nancsliicireii*itrwssyynw**tfplsrstfptiilgygvcwy
s*nnl*fnhem*c*ys*rfiw*cciirwynyvpryc*sye*rinctctinnen*nyct


Frame C:
llnnskdfnyililnk*tnkkknkkwmxkmfkl*llimvqvcvklvllvmmlhvlfshql
lvvqdtlvlwlvwvkrihmsxmklnqkevf*hlsiqlnmvlspigmiwkksgiilfimnf
xllxrniqfx*lklh*iqkqierk*LKSCLKLSTLQQCMLPFKLYYHSMPLVVPLVSLWI
QVMVFPILYQFTKGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKE
KLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGI
HETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAP
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFL261 (SFL261Q) /CSM/SF/SFL2-C/SFL261Q.Seq.d/                       1953   0.0
SFD790 (SFD790Q) /CSM/SF/SFD7-D/SFD790Q.Seq.d/                       1953   0.0
SFD319 (SFD319Q) /CSM/SF/SFD3-A/SFD319Q.Seq.d/                       1953   0.0
CHD357 (CHD357Q) /CSM/CH/CHD3-C/CHD357Q.Seq.d/                       1953   0.0
CFI221 (CFI221Q) /CSM/CF/CFI2-A/CFI221Q.Seq.d/                       1953   0.0
AFO539 (AFO539Q) /CSM/AF/AFO5-B/AFO539Q.Seq.d/                       1953   0.0
AFO158 (AFO158Q) /CSM/AF/AFO1-C/AFO158Q.Seq.d/                       1953   0.0
AFN538 (AFN538Q) /CSM/AF/AFN5-B/AFN538Q.Seq.d/                       1953   0.0
AFN507 (AFN507Q) /CSM/AF/AFN5-A/AFN507Q.Seq.d/                       1953   0.0
AFL134 (AFL134Q) /CSM/AF/AFL1-B/AFL134Q.Seq.d/                       1953   0.0
own update 2003. 5.16
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.    809   0.0    2
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   777   0.0    12
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   733   0.0    15
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   733   0.0    11
X03282|X03282.1 dictyostelium discoideum gene for actin A12.      702   0.0    2
X03284|X03284.1 Dictyostelium discoideum gene for actin A3-S2.    690   0.0    2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   670   0.0    2
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.         615   0.0    2
AC115680|AC115680.3 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   615   0.0    2
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   599   0.0    2
dna update 2004. 8.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   347   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   347   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        341   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   345   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   345   0.0
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   339   0.0
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          338   0.0
AY294152_1(AY294152|pid:none) Mayorella sp. JJP-2003 actin mRNA,...   339   e-180
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   338   e-179
AY294153_1(AY294153|pid:none) Platyamoeba placida actin mRNA, pa...   335   e-179
protein update 2009. 6.29
PSORT 

 psg: 0.82  gvh: 0.45  alm: 0.26  top: 0.53  tms: 0.07  mit: 0.41  mip: 0.10
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.38  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 20.0 %: endoplasmic reticulum
	 16.0 %: Golgi
	 12.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AFN538 is mit
5' end seq. ID AFN538F
5' end seq. 
>AFN538F.Seq
AATTATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATA
AAAAAAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTC
AGGTATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAAT
TGTTGGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGT
CGGNGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGG
TATTGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACT
TCGNGTTGCTCCANAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGC
AAATAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGT
TGCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTA----------
Length of 5' end seq. 521
3' end seq. ID AFN538Z
3' end seq. 
>AFN538Z.Seq
----------CAATGTATGTTGCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGT
ACCACTGGTATCGTTATGGATTCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAA
GGGTTATGCATTACCACATGCTATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGA
TTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACAACAACTGCTGAAAGAGA
AATCGTTAGAGATATTAAAGAGAAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAAT
GCAAACTGCAGCCTCATCATCTGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGT
TATTACAATTGGTAATGAACGTTTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTT
GGGTATGGAGTCTGCTGGTATTCATGAAACAACCTATAATTCAATCATGAAATGTGATGT
TGATATTCGTAAAGATTTATATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCC
AGGTATTGCTGATCGTATGAATAAAGAATTAACTGCACTTGCACCATCAACAATGAAAAT
TAAAATTATTGCACCAC
Length of 3' end seq. 607
Connected seq. ID AFN538P
Connected seq. 
>AFN538P.Seq
AATTATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATA
AAAAAAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTC
AGGTATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAAT
TGTTGGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGT
CGGNGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGG
TATTGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACT
TCGNGTTGCTCCANAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGC
AAATAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGT
TGCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTA----------CAATGTATG
TTGCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGG
ATTCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACCACAT
GCTATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTA
ACTGAACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATTAAA
GAGAAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCATCA
TCTGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATGAA
CGTTTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTGGT
ATTCATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTA
TATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATG
AATAAAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCAC
Length of connected seq. 1128
Full length Seq ID AFN538E
Full length Seq. 
>AFN538E.Seq
AATTATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATA
AAAAAAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTC
AGGTATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAAT
TGTTGGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGT
CGGNGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGG
TATTGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACT
TCGNGTTGCTCCANAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGC
AAATAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGT
TGCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGA
TTCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACCACATG
CTATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAA
CTGAACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATTAAAG
AGAAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCATCAT
CTGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATGAAC
GTTTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTGGTA
TTCATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTAT
ATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGA
ATAAAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCAC
Length of full length seq. 1077