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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHC247
Library VH
(Link to library)
Clone ID VHC247
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U12160-1
Original site URL
Representative seq. ID VHC247P
(Link to Original site)
Representative DNA sequence 
>VHC247 (VHC247Q) /CSM/VH/VHC2-B/VHC247Q.Seq.d/
TATAAACGATAAGGCATTCATCATTCAATTGTTTGATAATATTTTAAAGGCTTCTGATAT
ATCTGTACAAAAAATAATTGGTGATAAACCACCTCCAGCAACTATAACTCCAATTTTGCC
ACCTAAAACTGAAGTTACACAAACAACACCACCATCATCATCATCATCATCATCATCATC
ATCATCATCATCATCATCATCATCATCATCATCTACAACAAAAAATAACTCCACTACTAT
TGTAAATAACACTGATTCAACTAAATTTGTAGATGTAAAGAAAGAACAACTCACTACAGT
TCCAATTGTTAAGCAAGAATCAAATTCTCCATCAAAAACATTACCACCATCAAATTCTCC
ATCAAAAACATTACCACTATCAAATTCTCCATCAAAAACATTACTACCATCAAATTCATC
TATTCCCAATAAATCTACACCATCACCAATACCCAAACCAACTACATCTTCGACCACTTA
TCCAGTAACAACATCAAATCCATCTTCCAAACCTCAAACCCCAATTCCAACATCAAATAA
ATCACCCGCCAAATCGAATACTACCACAACTCCAGCAACCACAACCTCAAATACATCAAT
TACACCTACTCCCAAACCACTCACTCAAACAACCACTTCAACTTCAAXXXXXXXXXXTAG
TGGTTTAATACCTGGTAAAATGCCACCATCATTATCATCATCATCAGCAAACCCCATATC
AATTACAAACAATACAACTTCATTATCACAACAATCAAATACAACCAACACAATGCCTTC
AACTGTTTCACTTTCTAGTGGCAGTACTAGTATAAATTCCAATAGTAGCAATAGTAAATC
TTTAAGATCACCAAAATCATCTGATAACGATGGTAAAGAAAGTAAAAAAGAAAGAAAAGA
AAAGAATCTTGATAAAGAAAGCGGCAAATCAGATAGGGAAACTAAATCAGAAAAGAATGA
AAATAGAAAAAAAGATAAAAATCATGATAAAGATAGCAAATCTGATAAAAACCATGATAA
ATCTGATAAAAACAATAACGATAAATTTGACTCCAACAAAAATGATAACGGAGCAATTGT
AACTTCCCCATTCAACCTTTTATTCAATCCAGATAAAAGTCCAATTCCTTATAAACTATT
TATTGGTAAAAGAGAATTACTTTTAGAATGTAGAAGATTATTAAGAATTCAATGTTCAAG
AATTAGTGAATTAAATAAACAAAATATTGAAAGTGGAAAAGATACAACCGAAGTTATATC
AAAAATGAAAGATTTAATTAGTGAATTATCATCTTTTAAATTTTTGTTATTAGATATTGC
ATCGAAAGATATAAACTTTATAAAAAATCAATGGTATATAGATTTTTAATTTC
sequence update 2002.10.25
Translated Amino Acid sequence 
INDKAFIIQLFDNILKASDISVQKIIGDKPPPATITPILPPKTEVTQTTPPSSSSSSSSS
SSSSSSSSSSSTTKNNSTTIVNNTDSTKFVDVKKEQLTTVPIVKQESNSPSKTLPPSNSP
SKTLPLSNSPSKTLLPSNSSIPNKSTPSPIPKPTTSSTTYPVTTSNPSSKPQTPIPTSNK
SPAKSNTTTTPATTTSNTSITPTPKPLTQTTTSTS---

---SGLIPGKMPPSLSSSSANPISITNNTTSLSQQSNTTNTMPSTVSLSSGSTSINSNSS
NSKSLRSPKSSDNDGKESKKERKEKNLDKESGKSDRETKSEKNENRKKDKNHDKDSKSDK
NHDKSDKNNNDKFDSNKNDNGAIVTSPFNLLFNPDKSPIPYKLFIGKRELLLECRRLLRI
QCSRISELNKQNIESGKDTTEVISKMKDLISELSSFKFLLLDIASKDINFIKNQWYIDF*
f
Translated Amino Acid sequence (All Frames) 
Frame A:
ykr*gihhsiv**yfkgf*yictknnw**ttssnynsnfat*n*sytnnttiiiiiiiii
iiiiiiiiiiiynkk*lhyyck*h*fn*icrckertthyssnc*arikfsiknittikfs
iknittikfsiknittikfiysq*iytitntqtnyifdhlssnniksifqtsnpnsnik*
itrqieyyhnssnhnlkyinytysqtthsnnhfnf---

---*wfntw*natiiiiiiskphinykqynfiittikynqhnafncftf*wqy*ykfq**
q**ifkitkii**rw*rk*krkkrkes**rkrqir*gn*irke*k*kkr*ks**r*qi**
kp**i**kq*r*i*lqqk**rsncnfpiqpfiqsr*ksnsl*tiyw*kritfrm*kiikn
smfkn**ik*tky*kwkrynrsyiknerfn**iiif*ifvirycieryklykksmvyrfl
i

Frame B:
INDKAFIIQLFDNILKASDISVQKIIGDKPPPATITPILPPKTEVTQTTPPSSSSSSSSS
SSSSSSSSSSSTTKNNSTTIVNNTDSTKFVDVKKEQLTTVPIVKQESNSPSKTLPPSNSP
SKTLPLSNSPSKTLLPSNSSIPNKSTPSPIPKPTTSSTTYPVTTSNPSSKPQTPIPTSNK
SPAKSNTTTTPATTTSNTSITPTPKPLTQTTTSTS---

---SGLIPGKMPPSLSSSSANPISITNNTTSLSQQSNTTNTMPSTVSLSSGSTSINSNSS
NSKSLRSPKSSDNDGKESKKERKEKNLDKESGKSDRETKSEKNENRKKDKNHDKDSKSDK
NHDKSDKNNNDKFDSNKNDNGAIVTSPFNLLFNPDKSPIPYKLFIGKRELLLECRRLLRI
QCSRISELNKQNIESGKDTTEVISKMKDLISELSSFKFLLLDIASKDINFIKNQWYIDF*
f

Frame C:
*tirhssfncliif*rlliylykk*lvinhlqql*lqfchlklklhkqhhhhhhhhhhhh
hhhhhhhhhhlqqkitplll*itliqlnl*m*rknnslqfqllsknqilhqkhyhhqilh
qkhyhyqilhqkhyyhqihlfpinlhhhqypnqlhlrpliq*qhqihlpnlkpqfqhqin
hppnrilpqlqqpqpqihqlhllpnhslkqplqlq---

---vv*ylvkchhhyhhhqqtpyqlqtiqlhyhnnqiqptqclqlfhflvavlv*ipiva
ivnl*dhqnhlitmvkkvkkkekkrilikkaanqigklnqkrmkiekkikimikianlik
tminliktitinltptkmiteql*lphstfysiqikvqflinyllvkenyf*nvedy*ef
nvqelvn*inkilkvekiqpklyqk*ki*lvnyhllnfcy*ilhrki*tl*kingi*ifn
f
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC247 (VHC247Q) /CSM/VH/VHC2-B/VHC247Q.Seq.d/                       1251   0.0
SLE539 (SLE539Q) /CSM/SL/SLE5-B/SLE539Q.Seq.d/                        430   e-119
SLE321 (SLE321Q) /CSM/SL/SLE3-A/SLE321Q.Seq.d/                        430   e-119
SFH669 (SFH669Q) /CSM/SF/SFH6-C/SFH669Q.Seq.d/                         46   0.001
SHH457 (SHH457Q) /CSM/SH/SHH4-C/SHH457Q.Seq.d/                         42   0.022
SHE722 (SHE722Q) /CSM/SH/SHE7-A/SHE722Q.Seq.d/                         42   0.022
CHN862 (CHN862Q) /CSM/CH/CHN8-C/CHN862Q.Seq.d/                         42   0.022
CFI746 (CFI746Q) /CSM/CF/CFI7-B/CFI746Q.Seq.d/                         42   0.022
AFL219 (AFL219Q) /CSM/AF/AFL2-A/AFL219Q.Seq.d/                         42   0.022
VHP596 (VHP596Q) /CSM/VH/VHP5-D/VHP596Q.Seq.d/                         40   0.088
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BE972410|BE972410.1 601652231F1 NIH_MGC_82 Homo sapiens cDNA clone IMAGE:3935790 5',mRNA sequence.    30   0.002  3
AC025376|AC025376.2 Homo sapiens chromosome 1 clone RP11-262L17 map 1, WORKING DRAFT SEQUENCE, 26 unordered pieces.    30   0.006  3
AC106705|AC106705.7 Homo sapiens 3 BAC RP11-262L17 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    30   0.006  3
AC106780|AC106780.1 Homo sapiens chromosome 5 clone RP11-345O19, WORKING DRAFT SEQUENCE, 11 unordered pieces.    44   0.008  6
AM365642|AM365642.1 Oryzias latipes EST, clone FOE002-P00065-DPE-R_O19.    54   0.011  1
AM364387|AM364387.1 Oryzias latipes EST, clone FOE002-P00060-DPE-R_N15.    54   0.011  1
AM359107|AM359107.1 Oryzias latipes EST, clone FOE002-P00041-DPE-R_C10.    54   0.011  1
AM357684|AM357684.1 Oryzias latipes EST, clone FOE002-P00035-DPE-R_P19.    54   0.011  1
AM353632|AM353632.1 Oryzias latipes EST, clone FOE002-P00021-DPE-R_K14.    54   0.011  1
BJ745062|BJ745062.1 Oryzias latipes cDNA clone:MF015DA026d03, 3' end.    54   0.011  1
dna update 2007. 4.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54QC0) RecName: Full=Myb-like protein U;                            185   4e-45
AM910991_170(AM910991|pid:none) Plasmodium knowlesi strain H chr...    55   6e-06
S14983(S14983)extensin class I (clone w10-1 L) - tomato (fragment)     50   2e-04
AF362373_1(AF362373|pid:none) Dictyostelium discoideum histidine...    44   0.004
AC117075_53(AC117075|pid:none) Dictyostelium discoideum chromoso...    45   0.006
DQ378284_4(DQ378284|pid:none) Drosophila virilis strain Tucson 1...    45   0.006
(Q8MP30) RecName: Full=Uncharacterized histidine-rich protein DD...    45   0.008
(P04929) Histidine-rich glycoprotein precursor.  &KGZQHL(A22692)...    45   0.008
(P36417) RecName: Full=G-box-binding factor;          Short=GBF;       41   0.017
(Q54VU4) RecName: Full=Probable serine/threonine-protein kinase ...    44   0.018
protein update 2009. 6.28
PSORT 

 psg: 0.80  gvh: 0.39  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHC247 is cyt
5' end seq. ID VHC247F
5' end seq. 
>VHC247F.Seq
TATAAACGATAAGGCATTCATCATTCAATTGTTTGATAATATTTTAAAGGCTTCTGATAT
ATCTGTACAAAAAATAATTGGTGATAAACCACCTCCAGCAACTATAACTCCAATTTTGCC
ACCTAAAACTGAAGTTACACAAACAACACCACCATCATCATCATCATCATCATCATCATC
ATCATCATCATCATCATCATCATCATCATCATCTACAACAAAAAATAACTCCACTACTAT
TGTAAATAACACTGATTCAACTAAATTTGTAGATGTAAAGAAAGAACAACTCACTACAGT
TCCAATTGTTAAGCAAGAATCAAATTCTCCATCAAAAACATTACCACCATCAAATTCTCC
ATCAAAAACATTACCACTATCAAATTCTCCATCAAAAACATTACTACCATCAAATTCATC
TATTCCCAATAAATCTACACCATCACCAATACCCAAACCAACTACATCTTCGACCACTTA
TCCAGTAACAACATCAAATCCATCTTCCAAACCTCAAACCCCAATTCCAACATCAAATAA
ATCACCCGCCAAATCGAATACTACCACAACTCCAGCAACCACAACCTCAAATACATCAAT
TACACCTACTCCCAAACCACTCACTCAAACAACCACTTCAACTTCAANNNNNNNNNN
Length of 5' end seq. 657
3' end seq. ID VHC247Z
3' end seq. 
>VHC247Z.Seq
NNNNNNNNNNTAGTGGTTTAATACCTGGTAAAATGCCACCATCATTATCATCATCATCAG
CAAACCCCATATCAATTACAAACAATACAACTTCATTATCACAACAATCAAATACAACCA
ACACAATGCCTTCAACTGTTTCACTTTCTAGTGGCAGTACTAGTATAAATTCCAATAGTA
GCAATAGTAAATCTTTAAGATCACCAAAATCATCTGATAACGATGGTAAAGAAAGTAAAA
AAGAAAGAAAAGAAAAGAATCTTGATAAAGAAAGCGGCAAATCAGATAGGGAAACTAAAT
CAGAAAAGAATGAAAATAGAAAAAAAGATAAAAATCATGATAAAGATAGCAAATCTGATA
AAAACCATGATAAATCTGATAAAAACAATAACGATAAATTTGACTCCAACAAAAATGATA
ACGGAGCAATTGTAACTTCCCCATTCAACCTTTTATTCAATCCAGATAAAAGTCCAATTC
CTTATAAACTATTTATTGGTAAAAGAGAATTACTTTTAGAATGTAGAAGATTATTAAGAA
TTCAATGTTCAAGAATTAGTGAATTAAATAAACAAAATATTGAAAGTGGAAAAGATACAA
CCGAAGTTATATCAAAAATGAAAGATTTAATTAGTGAATTATCATCTTTTAAATTTTTGT
TATTAGATATTGCATCGAAAGATATAAACTTTATAAAAAATCAATGGTATATAGATTTTT
AATTTC
Length of 3' end seq. 726
Connected seq. ID VHC247P
Connected seq. 
>VHC247P.Seq
TATAAACGATAAGGCATTCATCATTCAATTGTTTGATAATATTTTAAAGGCTTCTGATAT
ATCTGTACAAAAAATAATTGGTGATAAACCACCTCCAGCAACTATAACTCCAATTTTGCC
ACCTAAAACTGAAGTTACACAAACAACACCACCATCATCATCATCATCATCATCATCATC
ATCATCATCATCATCATCATCATCATCATCATCTACAACAAAAAATAACTCCACTACTAT
TGTAAATAACACTGATTCAACTAAATTTGTAGATGTAAAGAAAGAACAACTCACTACAGT
TCCAATTGTTAAGCAAGAATCAAATTCTCCATCAAAAACATTACCACCATCAAATTCTCC
ATCAAAAACATTACCACTATCAAATTCTCCATCAAAAACATTACTACCATCAAATTCATC
TATTCCCAATAAATCTACACCATCACCAATACCCAAACCAACTACATCTTCGACCACTTA
TCCAGTAACAACATCAAATCCATCTTCCAAACCTCAAACCCCAATTCCAACATCAAATAA
ATCACCCGCCAAATCGAATACTACCACAACTCCAGCAACCACAACCTCAAATACATCAAT
TACACCTACTCCCAAACCACTCACTCAAACAACCACTTCAACTTCAA----------TAG
TGGTTTAATACCTGGTAAAATGCCACCATCATTATCATCATCATCAGCAAACCCCATATC
AATTACAAACAATACAACTTCATTATCACAACAATCAAATACAACCAACACAATGCCTTC
AACTGTTTCACTTTCTAGTGGCAGTACTAGTATAAATTCCAATAGTAGCAATAGTAAATC
TTTAAGATCACCAAAATCATCTGATAACGATGGTAAAGAAAGTAAAAAAGAAAGAAAAGA
AAAGAATCTTGATAAAGAAAGCGGCAAATCAGATAGGGAAACTAAATCAGAAAAGAATGA
AAATAGAAAAAAAGATAAAAATCATGATAAAGATAGCAAATCTGATAAAAACCATGATAA
ATCTGATAAAAACAATAACGATAAATTTGACTCCAACAAAAATGATAACGGAGCAATTGT
AACTTCCCCATTCAACCTTTTATTCAATCCAGATAAAAGTCCAATTCCTTATAAACTATT
TATTGGTAAAAGAGAATTACTTTTAGAATGTAGAAGATTATTAAGAATTCAATGTTCAAG
AATTAGTGAATTAAATAAACAAAATATTGAAAGTGGAAAAGATACAACCGAAGTTATATC
AAAAATGAAAGATTTAATTAGTGAATTATCATCTTTTAAATTTTTGTTATTAGATATTGC
ATCGAAAGATATAAACTTTATAAAAAATCAATGGTATATAGATTTTTAATTTC
Length of connected seq. 1363
Full length Seq ID -
Full length Seq. -
Length of full length seq. -