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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHC237
Library VH
(Link to library)
Clone ID VHC237
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16429-1
Original site URL
Representative seq. ID VHC237P
(Link to Original site)
Representative DNA sequence 
>VHC237 (VHC237Q) /CSM/VH/VHC2-B/VHC237Q.Seq.d/
ATTAATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTT
GATAAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCA
AAACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCA
GCAGTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAAT
TGGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAA
GATTTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGA
TTAGGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCAC
TCTGTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAA
TTCCATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGA
TTATTTATTGCTCCACCTGGAAGTGGTATTGTACATCAAGTAAATTTAGAGTATATTGCA
AGAGAAGTTATGAATGGAACTGGTAACCTCTTATATCCGXXXXXXXXXXCACTGATCATA
TTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAG
GTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGG
TTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCAC
AAACAACCTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATC
AATCAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTA
GAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCAT
TTGAACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAAC
CAGGTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTAC
CAACTGATAAATCCCTCATTAAAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTA
AATCTTTTGAAACTATCCTTCGTTTCGATACTCCAATCGAAGTTGAATATTGGGCAAATA
ATGGTATCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTTCTTTTTTTAATATAAA
ATAAAAAAAAATAATATTATTTAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
INFNLYKLPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLES
AVRNCDNFEVHEKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKR
LGGDPAKINPLVPVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDG
LFIAPPGSGIVHQVNLEYIAREVMNGTGNLLYP---

---TDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRIVNKL
APAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYLQGIK
CVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTGQTVK
VTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*yylk
Translated Amino Acid sequence (All Frames) 
Frame A:
INFNLYKLPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLES
AVRNCDNFEVHEKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKR
LGGDPAKINPLVPVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDG
LFIAPPGSGIVHQVNLEYIAREVMNGTGNLLYP---

---h*syftsr*hq*kiisrslfrvqrcktrrf*hlwis*r***nyg*rylcqysyc**i
gssrwstnnlctnw*vnvyfrccrkisirrssthcfgw**lwlw*f*rlgcqrsipsrn*
vchchli*tys*iqsrwygyypiaiptrskclyfgshw*rtiqyrvtn**iph*nwsnrk
gyh*lw*if*nypsfrysnrs*ilgk*wylilcfkktftlisffnik*kkiilfk

Frame B:
liliyinylit*r*qqiihlik*rmy*nhkikliifiifqnfktqelknyhtpleffwnq
qfvivitlkytkkmlkis*igktlqiklkflsnqqeyyykillvyqqllilqq*emp*kd
*vviqqklthlfqli*llitlfklmyqellml*skikrlnsieimkdfhf*ngvlkhlmd
ylllhlevvlyik*i*silqekl*melvtsyi---

---TDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRIVNKL
APAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYLQGIK
CVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTGQTVK
VTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*yylk

Frame C:
*f*fi*itlllkddnk*si**skgcikitrsnl*fl*sfktsrpkn*kitilh*nsfgis
ss*l**l*strkrc*kylklgkhck*s*nsfqtsksiitrfywctssc*scsnercheki
rw*sskn*ptcss*fsy*slcss*ciknc*cfrakskd*ip*ks*kififkmgcssi*wi
iycstwkwyctsskfrvyckrsyewnw*plis---

---liifhqpvtsienhqplvi*spkv*nqkiltpmdlvevmmklwlevplpilvllinw
lqplvhkqpmyqlvs*clfqmlqkninqkvinslfwlvvimalvvleiglpkvhtfkels
vslpshlnvfidpislvwvlshcnsnqvkmpllwvslvknnsissyqlinpslklvkp*r
lplivvnllklsfvsilqsklnigqimvsylmf*enfyinfff*ykikknnii*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC237 (VHC237Q) /CSM/VH/VHC2-B/VHC237Q.Seq.d/                       2527   0.0
VHQ461 (VHQ461Q) /CSM/VH/VHQ4-C/VHQ461Q.Seq.d/                       1300   0.0
VHQ432 (VHQ432Q) /CSM/VH/VHQ4-B/VHQ432Q.Seq.d/                       1300   0.0
VHQ267 (VHQ267Q) /CSM/VH/VHQ2-C/VHQ267Q.Seq.d/                       1300   0.0
VHQ262 (VHQ262Q) /CSM/VH/VHQ2-C/VHQ262Q.Seq.d/                       1300   0.0
VHP311 (VHP311Q) /CSM/VH/VHP3-A/VHP311Q.Seq.d/                       1300   0.0
VHO844 (VHO844Q) /CSM/VH/VHO8-B/VHO844Q.Seq.d/                       1300   0.0
VHO678 (VHO678Q) /CSM/VH/VHO6-D/VHO678Q.Seq.d/                       1300   0.0
VHK789 (VHK789Q) /CSM/VH/VHK7-D/VHK789Q.Seq.d/                       1300   0.0
VHK750 (VHK750Q) /CSM/VH/VHK7-C/VHK750Q.Seq.d/                       1300   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY250116|AY250116.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   7e-26  5
AF194945|AF194945.1 Nicotiana tabacum cytosolic aconitase (ACO) mRNA, complete cds.    54   1e-24  6
EJ685842|EJ685842.1 1092955177152 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061001864069 5', genomic survey sequence.    82   2e-24  3
AY250115|AY250115.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   3e-21  5
BE579347|BE579347.1 kq26c09.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:ZK455.1 CE03812 PROBABLE ACONITATE HYDRATASE, CYTOPLASMIC ;, mRNA sequence.    64   3e-20  3
CO910019|CO910019.1 BJ02055G09 BJ02 Capsicum annuum cDNA 5', mRNA sequence.    58   2e-18  4
EJ232220|EJ232220.1 1092404029403 Global-Ocean-Sampling_GS-27-01-01-1P8-2P0KB marine metagenome genomic clone 1061001509892 5', genomic survey sequence.    60   3e-18  4
CT817231|CT817231.1 Paramecium tetraurelia 5-PRIME EST from clone LK0AEA54YB14.    60   3e-18  3
CT783311|CT783311.1 Paramecium tetraurelia 5-PRIME EST from clone LK0ADA16YM13.    60   3e-18  3
CT816657|CT816657.1 Paramecium tetraurelia 5-PRIME EST from clone LK0AFA35YO17.    60   3e-18  3
dna update 2007. 4.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X73) RecName: Full=Probable cytoplasmic aconitate hydratase;...   436   e-120
AB236788_1(AB236788|pid:none) Trifolium pratense RNA for putativ...   284   4e-75
AP008209_256(AP008209|pid:none) Oryza sativa (japonica cultivar-...   283   1e-74
BT060820_1(BT060820|pid:none) Zea mays full-length cDNA clone ZM...   281   5e-74
(Q23500) RecName: Full=Probable cytoplasmic aconitate hydratase;...   280   6e-74
BT060665_1(BT060665|pid:none) Zea mays full-length cDNA clone ZM...   280   8e-74
EU816775_1(EU816775|pid:none) Perkinsus marinus aconitase-1 mRNA...   278   2e-73
(P49608) RecName: Full=Aconitate hydratase, cytoplasmic;        ...   277   7e-73
DQ294230_1(DQ294230|pid:none) Glossina morsitans morsitans TC165...   276   9e-73
(Q9SIB9) RecName: Full=Aconitate hydratase 2, mitochondrial;    ...   276   2e-72
protein update 2009. 6.28
PSORT 

 psg: 0.75  gvh: 0.20  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHC237 is cyt
5' end seq. ID VHC237F
5' end seq. 
>VHC237F.Seq
ATTAATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTT
GATAAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCA
AAACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCA
GCAGTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAAT
TGGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAA
GATTTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGA
TTAGGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCAC
TCTGTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAA
TTCCATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGA
TTATTTATTGCTCCACCTGGAAGTGGTATTGTACATCAAGTAAATTTAGAGTATATTGCA
AGAGAAGTTATGAATGGAACTGGTAACCTCTTATATCCGNNNNNNNNNN
Length of 5' end seq. 649
3' end seq. ID VHC237Z
3' end seq. 
>VHC237Z.Seq
NNNNNNNNNNCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCG
CTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTA
GAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAAT
TGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGTTAATGTTTATTT
CAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTG
ATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTA
AGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCGTTGGTATGGGTA
TTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAG
AACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTGGTCAAACCGTAA
AGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCGATACTCCAATCG
AAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAAAACTTTTACATT
AATTTCTTTTTTTAATATAAAATAAAAAAAAATAATATTATTTAAAA
Length of 3' end seq. 707
Connected seq. ID VHC237P
Connected seq. 
>VHC237P.Seq
ATTAATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTT
GATAAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCA
AAACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCA
GCAGTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAAT
TGGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAA
GATTTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGA
TTAGGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCAC
TCTGTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAA
TTCCATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGA
TTATTTATTGCTCCACCTGGAAGTGGTATTGTACATCAAGTAAATTTAGAGTATATTGCA
AGAGAAGTTATGAATGGAACTGGTAACCTCTTATATCCG----------CACTGATCATA
TTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAG
GTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGG
TTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCAC
AAACAACCTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATC
AATCAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTA
GAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCAT
TTGAACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAAC
CAGGTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTAC
CAACTGATAAATCCCTCATTAAAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTA
AATCTTTTGAAACTATCCTTCGTTTCGATACTCCAATCGAAGTTGAATATTGGGCAAATA
ATGGTATCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTTCTTTTTTTAATATAAA
ATAAAAAAAAATAATATTATTTAAAA
Length of connected seq. 1336
Full length Seq ID -
Full length Seq. -
Length of full length seq. -