>VHC226 (VHC226Q) /CSM/VH/VHC2-B/VHC226Q.Seq.d/
ATTTTTATAATTTCACTTGTTTATTTTAATTTTATACATATAAATAATAATATAGTTGAA
AAAATGTGTGATCATAAACATTTAGTTGACAAAGTAGATAGGTTAGTATTACCAGATAAT
ATCGTACCAAGTAAATACGATTTACATCTTAGACCAAATTTAAAGGATTTTGTATTCAGC
GGCAAAGTTGACATTACAATCAATATTGTTAAACCAACCAAAAAAATCATTATCCATTCA
ATTGACATTGAAATTCAATCAGTAAAGATCTTAAATCAACAAGCTACTTCCGTTACCTAC
TATGAACCAGAAGAAGTTGCAATTTTAGAATTCCAAGATGAATTACCAGTTACAGAAAAT
ACAATTCTCTCCATTGATTTCACTGGTATTTTAAATGACAAATTAAAAGGTTTCTATCGT
TCAAAATATGTTGTCAATGGTGAAGATCGTTATATTGGTACCACTCAATTCGAAGCCACT
GATGCTCGTCGTGCTTTCCCATGTTTTGATGAACCTGCTTTAAAATCTTTCTTTAATATT
AAAATCACTATTTCATCTCATTTAACTGCTCTTTCAAATATGGATACAACTTCTGTAATT
GAAAATAATGATGGTACXXXXXXXXXXTGTTAAGAGATAAAGTAAACACTAAATTGGGTA
TGTTGGGTGATGCTGATATCGTTGCTGAATGTAAGAAAAGATTCGAAGCTTTCAAAGCAG
ACTCTTCAAGTTTACCATCAGATATTCGTTCTACAGTACTTGCTACAATCGTAAAGAATG
GTGGTGAAGCTGATCAACAAATCATCATTGATCAATATCTTAAATCTTCTTTAGTCGCTG
AAAAGAATTCTTACCTTTTGGTACTTGCTTTGGCTCCAAAAGAGGAATTGGTTGAAAAAG
CCCTCAATTTCGCTTTATCTCCATCCGTTAGAAGTCAAGATAGTTATATGGTTTTCTTTA
CCCTCCCAAATCGTGTTCGTCAATTCGCTTGGGCTTACCTCACCAAAAACTTTAATCAAA
TCAACGAAACATTTAAAAACTCTCCTTTGTTTGGTCGTATCATTGGTAGTTGTCTCACCT
CTAAAATGGATGATTCTCAATACAATGAAGTCGTCAACTTTTTCAAAGATCATCCAGTTC
CAATTGCTGATCGTTCAATTAAACAAGATTTAGAAATGGTTACAATTAATTCAAATTGGT
TCAAAGCTTTTAATCAAGATTTATCAAATTGGATTCAATCAAAATAAAAATAAAATCTCT
TACAATGAAAGATCTTTGTTTTAATAATTGATAGNATAAAAAAAAAGAGACGAAAACTAA
AATAAACATCAAATTATTGTTTTCCTTTTAAAA

IFIISLVYFNFIHINNNIVEKMCDHKHLVDKVDRLVLPDNIVPSKYDLHLRPNLKDFVFS
GKVDITINIVKPTKKIIIHSIDIEIQSVKILNQQATSVTYYEPEEVAILEFQDELPVTEN
TILSIDFTGILNDKLKGFYRSKYVVNGEDRYIGTTQFEATDARRAFPCFDEPALKSFFNI
KITISSHLTALSNMDTTSVIENNDG---

---LRDKVNTKLGMLGDADIVAECKKRFEAFKADSSSLPSDIRSTVLATIVKNGGEADQQ
IIIDQYLKSSLVAEKNSYLLVLALAPKEELVEKALNFALSPSVRSQDSYMVFFTLPNRVR
QFAWAYLTKNFNQINETFKNSPLFGRIIGSCLTSKMDDSQYNEVVNFFKDHPVPIADRSI
KQDLEMVTINSNWFKAFNQDLSNWIQSK*k*nllq*kifvliidxikkkrrklk*tsnyc
fpfk

Frame A:
IFIISLVYFNFIHINNNIVEKMCDHKHLVDKVDRLVLPDNIVPSKYDLHLRPNLKDFVFS
GKVDITINIVKPTKKIIIHSIDIEIQSVKILNQQATSVTYYEPEEVAILEFQDELPVTEN
TILSIDFTGILNDKLKGFYRSKYVVNGEDRYIGTTQFEATDARRAFPCFDEPALKSFFNI
KITISSHLTALSNMDTTSVIENNDG---

---c*eik*tlnwvcwvmlisllnvrkdsklskqtlqvyhqifvlqyllqs*rmvvklin
ksslinilnll*slkriltfwyllwlqkrnwlkkpsislylhplevkiviwfslpsqivf
vnslgltspktlikstkhlktllclvvslvvvsplkwmilntmksstfskiiqfqllivq
lnki*kwlqliqigskllikiyqigfnqnknkisynerslf**lix*kkrden*nkhqii
vfllk

Frame B:
fl*fhlfililyi*iii*lkkcviini*ltk*ig*yyqiisyqvntiyildqi*rilysa
akltlqsillnqpkkslsiqltlkfnq*rs*inkllplptmnqkklqf*nskmnyqlqki
qfsplislvf*mtn*kvsivqnmlsmvkivilvplnskplmlvvlshvlmnll*nlslil
kslfhli*llfqiwiqll*lkimmv---

---vkr*skh*igyvg*c*yrc*m*ekirsfqsrlfkftirysfystcynrkeww*s*st
nhh*sis*iffsr*keflpfgtcfgskrgig*kspqfrfisir*ksr*lygflyppkscs
sirlglphqkl*snqrni*klsfvwsyhw*lshl*ng*fsiq*srqlfqrssssnc*sfn
*trfrngyn*fklvqsf*srfikldsikikiksltmkdlcfnn**xkkketktkinikll
fsf*

Frame C:
fynftclf*fytyk**ys*knv*s*tfs*qsr*vsitr*yrtk*irfts*tkfkgfciqr
qs*hynqyc*tnqknhypfn*h*nsiskdlkstsyfryll*trrscnfripr*itsyrky
nslh*fhwyfk*qikrflsfkiccqw*rslywyhsirsh*csscfpmf**tcfkifl*y*
nhyfisfncsfkygynfcn*k**wy---

---LRDKVNTKLGMLGDADIVAECKKRFEAFKADSSSLPSDIRSTVLATIVKNGGEADQQ
IIIDQYLKSSLVAEKNSYLLVLALAPKEELVEKALNFALSPSVRSQDSYMVFFTLPNRVR
QFAWAYLTKNFNQINETFKNSPLFGRIIGSCLTSKMDDSQYNEVVNFFKDHPVPIADRSI
KQDLEMVTINSNWFKAFNQDLSNWIQSK*k*nllq*kifvliidxikkkrrklk*tsnyc
fpfk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ164 (VHQ164Q) /CSM/VH/VHQ1-C/VHQ164Q.Seq.d/                       1380   0.0
VHP622 (VHP622Q) /CSM/VH/VHP6-A/VHP622Q.Seq.d/                       1380   0.0
VHK646 (VHK646Q) /CSM/VH/VHK6-B/VHK646Q.Seq.d/                       1380   0.0
VHK250 (VHK250Q) /CSM/VH/VHK2-C/VHK250Q.Seq.d/                       1380   0.0
VHK144 (VHK144Q) /CSM/VH/VHK1-B/VHK144Q.Seq.d/                       1380   0.0
VHJ580 (VHJ580Q) /CSM/VH/VHJ5-D/VHJ580Q.Seq.d/                       1380   0.0
VHJ119 (VHJ119Q) /CSM/VH/VHJ1-A/VHJ119Q.Seq.d/                       1380   0.0
VHP237 (VHP237Q) /CSM/VH/VHP2-B/VHP237Q.Seq.d/                       1378   0.0
VHP167 (VHP167Q) /CSM/VH/VHP1-C/VHP167Q.Seq.d/                       1366   0.0
VHP192 (VHP192Q) /CSM/VH/VHP1-D/VHP192Q.Seq.d/                       1364   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY631393|AY631393.1 Dictyostelium discoideum puromycin-sensitive aminopeptidase-like protein (psa) mRNA, partial cds.    80   3e-17  3
CR382136|CR382136.1 Debaryomyces hansenii chromosome D of strain CBS767 of Debaryomyces hansenii.    56   0.003  1
CP000498|CP000498.1 Pichia stipitis CBS 6054 chromosome 4, complete sequence.    38   0.006  2
CR382123|CR382123.1 Kluyveromyces lactis strain NRRL Y-1140 chromosome C of strain NRRL  Y-1140 of Kluyveromyces lactis.    54   0.011  1
DN815134|AF148323_1 aminopeptidase PILS [Rattus norvegicus], mRNA sequence.    52   0.042  1
BP514490|BP514490.1 Hydra magnipapillata cDNA, clone:hydmg018bw_14.    52   0.042  1
AM466448|AM466448.1 Vitis vinifera, whole genome shotgun sequence, contig VV79X000169.2, clone ENTAV 115.    46   0.056  3
AJ808281|AJ808281.1 Antirrhinum majus EST, clone 018_6_09_c24.     50   0.17   1
CP000123|CP000123.1 Mycoplasma capricolum subsp. capricolum ATCC 27343, complete genome.    42   0.45   17
CV966447|CV966447.1 PC007G7 infected tomato, center of lesion 3 dpi Phytophthora infestans cDNA, mRNA sequence.    36   0.48   2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY631393_1(AY631393|pid:none) Dictyostelium discoideum puromycin...   214   6e-54
AF327435_1(AF327435|pid:none) Drosophila melanogaster puromycin-...   148   4e-34
AE014296_282(AE014296|pid:none) Drosophila melanogaster chromoso...   148   5e-34
AE014296_281(AE014296|pid:none) Drosophila melanogaster chromoso...   148   5e-34
AM270075_67(AM270075|pid:none) Aspergillus niger contig An04c014...   132   2e-29
AP007157_574(AP007157|pid:none) Aspergillus oryzae RIB40 genomic...   130   1e-28
EF678434_1(EF678434|pid:none) Picea sitchensis clone WS02921_A14...   129   2e-28
EF676179_1(EF676179|pid:none) Picea sitchensis clone WS02717_G19...   128   4e-28
AP005572_11(AP005572|pid:none) Oryza sativa Japonica Group genom...   127   7e-28
AM920433_347(AM920433|pid:none) Penicillium chrysogenum Wisconsi...   126   2e-27

 psg: 0.95  gvh: 0.36  alm: 0.41  top: 0.57  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial

>> prediction for VHC226 is cyt

>VHC226F.Seq
ATTTTTATAATTTCACTTGTTTATTTTAATTTTATACATATAAATAATAATATAGTTGAA
AAAATGTGTGATCATAAACATTTAGTTGACAAAGTAGATAGGTTAGTATTACCAGATAAT
ATCGTACCAAGTAAATACGATTTACATCTTAGACCAAATTTAAAGGATTTTGTATTCAGC
GGCAAAGTTGACATTACAATCAATATTGTTAAACCAACCAAAAAAATCATTATCCATTCA
ATTGACATTGAAATTCAATCAGTAAAGATCTTAAATCAACAAGCTACTTCCGTTACCTAC
TATGAACCAGAAGAAGTTGCAATTTTAGAATTCCAAGATGAATTACCAGTTACAGAAAAT
ACAATTCTCTCCATTGATTTCACTGGTATTTTAAATGACAAATTAAAAGGTTTCTATCGT
TCAAAATATGTTGTCAATGGTGAAGATCGTTATATTGGTACCACTCAATTCGAAGCCACT
GATGCTCGTCGTGCTTTCCCATGTTTTGATGAACCTGCTTTAAAATCTTTCTTTAATATT
AAAATCACTATTTCATCTCATTTAACTGCTCTTTCAAATATGGATACAACTTCTGTAATT
GAAAATAATGATGGTACNNNNNNNNNN

>VHC226Z.Seq
NNNNNNNNNNTGTTAAGAGATAAAGTAAACACTAAATTGGGTATGTTGGGTGATGCTGAT
ATCGTTGCTGAATGTAAGAAAAGATTCGAAGCTTTCAAAGCAGACTCTTCAAGTTTACCA
TCAGATATTCGTTCTACAGTACTTGCTACAATCGTAAAGAATGGTGGTGAAGCTGATCAA
CAAATCATCATTGATCAATATCTTAAATCTTCTTTAGTCGCTGAAAAGAATTCTTACCTT
TTGGTACTTGCTTTGGCTCCAAAAGAGGAATTGGTTGAAAAAGCCCTCAATTTCGCTTTA
TCTCCATCCGTTAGAAGTCAAGATAGTTATATGGTTTTCTTTACCCTCCCAAATCGTGTT
CGTCAATTCGCTTGGGCTTACCTCACCAAAAACTTTAATCAAATCAACGAAACATTTAAA
AACTCTCCTTTGTTTGGTCGTATCATTGGTAGTTGTCTCACCTCTAAAATGGATGATTCT
CAATACAATGAAGTCGTCAACTTTTTCAAAGATCATCCAGTTCCAATTGCTGATCGTTCA
ATTAAACAAGATTTAGAAATGGTTACAATTAATTCAAATTGGTTCAAAGCTTTTAATCAA
GATTTATCAAATTGGATTCAATCAAAATAAAAATAAAATCTCTTACAATGAAAGATCTTT
GTTTTAATAATTGATAGNATAAAAAAAAAGAGACGAAAACTAAAATAAACATCAAATTAT
TGTTTTCCTTTTAAAA

>VHC226P.Seq
ATTTTTATAATTTCACTTGTTTATTTTAATTTTATACATATAAATAATAATATAGTTGAA
AAAATGTGTGATCATAAACATTTAGTTGACAAAGTAGATAGGTTAGTATTACCAGATAAT
ATCGTACCAAGTAAATACGATTTACATCTTAGACCAAATTTAAAGGATTTTGTATTCAGC
GGCAAAGTTGACATTACAATCAATATTGTTAAACCAACCAAAAAAATCATTATCCATTCA
ATTGACATTGAAATTCAATCAGTAAAGATCTTAAATCAACAAGCTACTTCCGTTACCTAC
TATGAACCAGAAGAAGTTGCAATTTTAGAATTCCAAGATGAATTACCAGTTACAGAAAAT
ACAATTCTCTCCATTGATTTCACTGGTATTTTAAATGACAAATTAAAAGGTTTCTATCGT
TCAAAATATGTTGTCAATGGTGAAGATCGTTATATTGGTACCACTCAATTCGAAGCCACT
GATGCTCGTCGTGCTTTCCCATGTTTTGATGAACCTGCTTTAAAATCTTTCTTTAATATT
AAAATCACTATTTCATCTCATTTAACTGCTCTTTCAAATATGGATACAACTTCTGTAATT
GAAAATAATGATGGTAC----------TGTTAAGAGATAAAGTAAACACTAAATTGGGTA
TGTTGGGTGATGCTGATATCGTTGCTGAATGTAAGAAAAGATTCGAAGCTTTCAAAGCAG
ACTCTTCAAGTTTACCATCAGATATTCGTTCTACAGTACTTGCTACAATCGTAAAGAATG
GTGGTGAAGCTGATCAACAAATCATCATTGATCAATATCTTAAATCTTCTTTAGTCGCTG
AAAAGAATTCTTACCTTTTGGTACTTGCTTTGGCTCCAAAAGAGGAATTGGTTGAAAAAG
CCCTCAATTTCGCTTTATCTCCATCCGTTAGAAGTCAAGATAGTTATATGGTTTTCTTTA
CCCTCCCAAATCGTGTTCGTCAATTCGCTTGGGCTTACCTCACCAAAAACTTTAATCAAA
TCAACGAAACATTTAAAAACTCTCCTTTGTTTGGTCGTATCATTGGTAGTTGTCTCACCT
CTAAAATGGATGATTCTCAATACAATGAAGTCGTCAACTTTTTCAAAGATCATCCAGTTC
CAATTGCTGATCGTTCAATTAAACAAGATTTAGAAATGGTTACAATTAATTCAAATTGGT
TCAAAGCTTTTAATCAAGATTTATCAAATTGGATTCAATCAAAATAAAAATAAAATCTCT
TACAATGAAAGATCTTTGTTTTAATAATTGATAGNATAAAAAAAAAGAGACGAAAACTAA
AATAAACATCAAATTATTGTTTTCCTTTTAAAA