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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHC225
Library VH
(Link to library)
Clone ID VHC225
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15527-1
Original site URL
Representative seq. ID VHC225P
(Link to Original site)
Representative DNA sequence 
>VHC225 (VHC225Q) /CSM/VH/VHC2-B/VHC225Q.Seq.d/
AACAAATAAAAATAAATACAAAAAATGAATTGTGGTTCAAAATATACTTTTGTTCAAGAT
GATGAAGAGGAAGAATTTGTTGTTGATAGCAATATTTTCTTTCAAGGTGCTCAACCTCAA
CAACCTCAACAACCTCAACAACCTCAACAACCTCAACAACAACAACAACAACAACAACAA
CAACAACAACAACAACAACAACAACAACAACAACAACAATCAAGAGGATTCTTTAGTATT
TTTGGTAGATCAGCAGCACCACAAGCACCACCACCAGCACCAATGAATAATCGTGCACCA
AATCAACTTAAAGCTAAAATAAAGAAAGCAACAACCAATGTATTAGTAGTCAATTTAGGT
ACAGTTGCAAAGGAATCATCTATTCAAACTGGTGATGGTTACTTTTGTAAAAAATGTAAT
TGTGGTTTCAGCAATATCAATGCATTAAAAGAGATAACATTAAATGATGGTAAAAAGCAT
CXXXXXXXXXXAGCAACAAATGTATCCGTTAAGATATTCCTCCATAAAGGTCTTTACGTT
AGTGACTTTGATAATAATAATAATACTGAAAAGAGTTTTGTTGAAAGAGTGATTGGTAAT
GTAAATCAAGACNCAACAATTTCATTTGAATATGGTGTTAGATCCTCTNAATTAATTGAT
AAATCTTTGAAATCAATACCATTCCAATTACAAATCTTTTATACAACNCTCANTGGTAAG
AAATGTGTAAGAATGATCACTCAAACTCAAGAGATTACANCAAGCTNTGATTTGGCTGAA
GAACATGCAAACATCGAGGCATTGGGTATTAATATGGTTCAANCCTCTACAAAGANTGCT
TCATCAGGTGATTATGAANAATCTCGTGCTAANAATTATTCACATGCTGGACTTATGCAA
AAGGTAATGATGAAGAAACAAAAGGCTACTAAAGTCGATTCAGATGAAATCGATTCAATG
TCAAATTATATGCA
sequence update 2002.10.25
Translated Amino Acid sequence 
nk*k*IQKMNCGSKYTFVQDDEEEEFVVDSNIFFQGAQPQQPQQPQQPQQPQQQQQQQQQ
QQQQQQQQQQQQQSRGFFSIFGRSAAPQAPPPAPMNNRAPNQLKAKIKKATTNVLVVNLG
TVAKESSIQTGDGYFCKKCNCGFSNINALKEITLNDGKKH---

---ATNVSVKIFLHKGLYVSDFDNNNNTEKSFVERVIGNVNQDXTISFEYGVRSSXLIDK
SLKSIPFQLQIFYTTLXGKKCVRMITQTQEITXSXDLAEEHANIEALGINMVQXSTKXAS
SGDYEXSRAXNYSHAGLMQKVMMKKQKATKVDSDEIDSMSNYM
Translated Amino Acid sequence (All Frames) 
Frame A:
nk*k*IQKMNCGSKYTFVQDDEEEEFVVDSNIFFQGAQPQQPQQPQQPQQPQQQQQQQQQ
QQQQQQQQQQQQQSRGFFSIFGRSAAPQAPPPAPMNNRAPNQLKAKIKKATTNVLVVNLG
TVAKESSIQTGDGYFCKKCNCGFSNINALKEITLNDGKKH---

---snkcir*dipp*rslr**l*****y*kefc*ksdw*cksrxnnfi*iwc*ilxin**
ifeintipitnllynxxw*emckndhsnsrdyxkl*fg*rtckhrgigy*ygsxlykxcf
ir*l*xisc*xlftcwtyakgndeetkgy*srfr*nrfnvklya

Frame B:
tnknkykk*ivvqnillfkmmkrknllliaifsfkvlnlnnlnnlnnlnnlnnnnnnnnn
nnnnnnnnnnnnnqedslvflvdqqhhkhhhqhq*iivhqinlklk*rkqqpmy**si*v
qlqrnhlfklvmvtfvknvivvsaismh*kr*h*mmvksi---

---ATNVSVKIFLHKGLYVSDFDNNNNTEKSFVERVIGNVNQDXTISFEYGVRSSXLIDK
SLKSIPFQLQIFYTTLXGKKCVRMITQTQEITXSXDLAEEHANIEALGINMVQXSTKXAS
SGDYEXSRAXNYSHAGLMQKVMMKKQKATKVDSDEIDSMSNYM

Frame C:
qikintknelwfkiyfcsr**rgricc**qyflsrcststtsttsttsttsttttttttt
ttttttttttttikril*yfw*issttstttstne*sctkst*s*nkesnnqcissqfry
sckgiiysnw*wlll*km*lwfqqyqcikrdnik*w*ka---

---qqmyplryssikvftlvtliiiiilkrvllke*lvm*iktqqfhlnmvldpln*lin
l*nqyhsnyksfiqxsxvrnv*e*slklkrlxqaxiwlknmqtsrhwvliwfxplqrxlh
qvimxnlvlxiihmldlckr***rnkrllksiqmksiqcqiic
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC225 (VHC225Q) /CSM/VH/VHC2-B/VHC225Q.Seq.d/                       1378   0.0
VHN609 (VHN609Q) /CSM/VH/VHN6-A/VHN609Q.Seq.d/                        898   0.0
VHK228 (VHK228Q) /CSM/VH/VHK2-B/VHK228Q.Seq.d/                        898   0.0
VHH660 (VHH660Q) /CSM/VH/VHH6-C/VHH660Q.Seq.d/                        898   0.0
VHD313 (VHD313Q) /CSM/VH/VHD3-A/VHD313Q.Seq.d/                        898   0.0
VHC281 (VHC281Q) /CSM/VH/VHC2-D/VHC281Q.Seq.d/                        898   0.0
VHA146 (VHA146Q) /CSM/VH/VHA1-B/VHA146Q.Seq.d/                        898   0.0
CHH115 (CHH115Q) /CSM/CH/CHH1-A/CHH115Q.Seq.d/                        898   0.0
AHE161 (AHE161Q) /CSM/AH/AHE1-C/AHE161Q.Seq.d/                        898   0.0
AFM666 (AFM666Q) /CSM/AF/AFM6-C/AFM666Q.Seq.d/                        898   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

EC786532|EC786532.1 RAE00017951 Reclinomonas americana Home made, normalized (normalized_ran) Reclinomonas americana cDNA, mRNA sequence.    58   5e-04  1
EC791898|EC791898.1 RAE00014301 Reclinomonas americana Home made, normalized (normalized_ran) Reclinomonas americana cDNA, mRNA sequence.    56   0.002  1
AC112559|AC112559.4 Rattus norvegicus clone CH230-204L20, WORKING DRAFT SEQUENCE, 2 unordered pieces.    56   0.002  1
AC103029|AC103029.6 Rattus norvegicus clone CH230-203F15, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    54   0.008  1
CL513863|CL513863.1 SAIL_880_C10.v2 SAIL Collection Arabidopsis thaliana genomic clone SAIL_880_C10.v2, genomic survey sequence.    54   0.008  1
AL161591|AL161591.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 87.    54   0.008  1
AL035601|AL035601.1 Arabidopsis thaliana DNA chromosome 4, BAC clone  F6G17 (ESSA  project).    54   0.008  1
EC383579| ring finger protein 10 [Homo sapiens], mRNA sequence.    52   0.030  1
AC098126|AC098126.7 Rattus norvegicus clone CH230-134H19, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    50   0.037  4
EH015744|EH015744.1 USDA-FP_188314 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt084_H05 5', mRNA sequence.    34   0.045  3
dna update 2007. 4.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q75J93) RecName: Full=Circularly permutated Ras protein 1;     ...    57   1e-06
protein update 2009. 6.28
PSORT 

 psg: 0.51  gvh: 0.54  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHC225 is cyt
5' end seq. ID VHC225F
5' end seq. 
>VHC225F.Seq
AACAAATAAAAATAAATACAAAAAATGAATTGTGGTTCAAAATATACTTTTGTTCAAGAT
GATGAAGAGGAAGAATTTGTTGTTGATAGCAATATTTTCTTTCAAGGTGCTCAACCTCAA
CAACCTCAACAACCTCAACAACCTCAACAACCTCAACAACAACAACAACAACAACAACAA
CAACAACAACAACAACAACAACAACAACAACAACAACAATCAAGAGGATTCTTTAGTATT
TTTGGTAGATCAGCAGCACCACAAGCACCACCACCAGCACCAATGAATAATCGTGCACCA
AATCAACTTAAAGCTAAAATAAAGAAAGCAACAACCAATGTATTAGTAGTCAATTTAGGT
ACAGTTGCAAAGGAATCATCTATTCAAACTGGTGATGGTTACTTTTGTAAAAAATGTAAT
TGTGGTTTCAGCAATATCAATGCATTAAAAGAGATAACATTAAATGATGGTAAAAAGCAT
CNNNNNNNNNN
Length of 5' end seq. 491
3' end seq. ID VHC225Z
3' end seq. 
>VHC225Z.Seq
NNNNNNNNNNAGCAACAAATGTATCCGTTAAGATATTCCTCCATAAAGGTCTTTACGTTA
GTGACTTTGATAATAATAATAATACTGAAAAGAGTTTTGTTGAAAGAGTGATTGGTAATG
TAAATCAAGACNCAACAATTTCATTTGAATATGGTGTTAGATCCTCTNAATTAATTGATA
AATCTTTGAAATCAATACCATTCCAATTACAAATCTTTTATACAACNCTCANTGGTAAGA
AATGTGTAAGAATGATCACTCAAACTCAAGAGATTACANCAAGCTNTGATTTGGCTGAAG
AACATGCAAACATCGAGGCATTGGGTATTAATATGGTTCAANCCTCTACAAAGANTGCTT
CATCAGGTGATTATGAANAATCTCGTGCTAANAATTATTCACATGCTGGACTTATGCAAA
AGGTAATGATGAAGAAACAAAAGGCTACTAAAGTCGATTCAGATGAAATCGATTCAATGT
CAAATTATATGCA
Length of 3' end seq. 493
Connected seq. ID VHC225P
Connected seq. 
>VHC225P.Seq
AACAAATAAAAATAAATACAAAAAATGAATTGTGGTTCAAAATATACTTTTGTTCAAGAT
GATGAAGAGGAAGAATTTGTTGTTGATAGCAATATTTTCTTTCAAGGTGCTCAACCTCAA
CAACCTCAACAACCTCAACAACCTCAACAACCTCAACAACAACAACAACAACAACAACAA
CAACAACAACAACAACAACAACAACAACAACAACAACAATCAAGAGGATTCTTTAGTATT
TTTGGTAGATCAGCAGCACCACAAGCACCACCACCAGCACCAATGAATAATCGTGCACCA
AATCAACTTAAAGCTAAAATAAAGAAAGCAACAACCAATGTATTAGTAGTCAATTTAGGT
ACAGTTGCAAAGGAATCATCTATTCAAACTGGTGATGGTTACTTTTGTAAAAAATGTAAT
TGTGGTTTCAGCAATATCAATGCATTAAAAGAGATAACATTAAATGATGGTAAAAAGCAT
C----------AGCAACAAATGTATCCGTTAAGATATTCCTCCATAAAGGTCTTTACGTT
AGTGACTTTGATAATAATAATAATACTGAAAAGAGTTTTGTTGAAAGAGTGATTGGTAAT
GTAAATCAAGACNCAACAATTTCATTTGAATATGGTGTTAGATCCTCTNAATTAATTGAT
AAATCTTTGAAATCAATACCATTCCAATTACAAATCTTTTATACAACNCTCANTGGTAAG
AAATGTGTAAGAATGATCACTCAAACTCAAGAGATTACANCAAGCTNTGATTTGGCTGAA
GAACATGCAAACATCGAGGCATTGGGTATTAATATGGTTCAANCCTCTACAAAGANTGCT
TCATCAGGTGATTATGAANAATCTCGTGCTAANAATTATTCACATGCTGGACTTATGCAA
AAGGTAATGATGAAGAAACAAAAGGCTACTAAAGTCGATTCAGATGAAATCGATTCAATG
TCAAATTATATGCA
Length of connected seq. 964
Full length Seq ID -
Full length Seq. -
Length of full length seq. -