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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFN530
Library AF
(Link to library)
Clone ID AFN530
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13881-1
Original site URL
Representative seq. ID AFN530P
(Link to Original site)
Representative DNA sequence 
>AFN530 (AFN530Q) /CSM/AF/AFN5-B/AFN530Q.Seq.d/
AATAAAAACTCAAAACAACACACACTTACACAATATATTTACTATTTTAAATTATATCTA
AATCCGACAACAACAATAACAATAACAACAACAACCCATTCTTTAATATACATATAAATA
TAAAATAATGAATCCAGATTTCACAGTTTATGTTTCAAATATTTCATTGAAAGCAAATAC
TAAAACAGTTTCAGATTTTTTCTCATTTTGTGGAAGAATTGTTAATTTATTTTTAAGAAA
TGACCCAACTGGTAGTTCACAACAAGCAATAGTAGTATTTGAATCAGATTCAGCTGCAAA
AACAGCTTTATTATTAACAAATGCATTAATTGTAGATAAAGTAATTCAAGTTGTTCAATT
TACACCAGAATTAGAGTTTGATTTAACACAACAATTTTCTCAACAACAACAATTTAATAA
TCTCAGTGGTGAACAACAACCACAACAACAACAATATGTTAGTCAACCACAAGAAGACAT
TGTAAATAGAGAACATAGTGTGCCAXXXXXXXXXXTANNGNNNNTANTGNNNNNNNNAAT
NAANGNAGTNNNNCNTNNGNTGNTGNNTCAATTNNTGCNGCAGGTTATAGTGTTGNNCAA
GNNGNNGCCATTAAAGCNCGTCAAGTTGATGAAGNNCATATGATTTCATTANNNCTTAAA
GTTGGAGCTGAAGCTGTCAAAGCAAAAGCAAATGAAATTGATAATAACCTTCACATTTNT
GAGAGTGCCGCTGCCATTAAGGGTGCAGTTGTAGAGAGAGCAAATGCACTNGATGAAAGA
TTCCAAATCTCAGGTTTCTTTAAGAGTGCAAGTGATATGATCTCTCAGCNAGCTTCCTAT
TTAATGAAAGCAGCCGAAGAAAATAGTACAGTTTCACCAATCGTAAATAAAGTTTC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
iktqnnthlhniftilnyi*irqqq*q*qqqpil*YTYKYKIMNPDFTVYVSNISLKANT
KTVSDFFSFCGRIVNLFLRNDPTGSSQQAIVVFESDSAAKTALLLTNALIVDKVIQVVQF
TPELEFDLTQQFSQQQQFNNLSGEQQPQQQQYVSQPQEDIVNREHSVP---

---XXXXXXXNXXSXXXXXXSIXAAGYSVXQXXAIKARQVDEXHMISLXLKVGAEAVKAK
ANEIDNNLHIXESAAAIKGAVVERANALDERFQISGFFKSASDMISQXASYLMKAAEENS
TVSPIVNKV
Translated Amino Acid sequence (All Frames) 
Frame A:
nknskqhtltqyiyyfklylnptttititttthsliyi*i*nnesrfhslcfkyfiesky
*nsfrfflilwknc*fifkk*pnw*fttsnssi*irfscknsfiinkcincr*snsscsi
ytrirv*fnttifsttti**sqw*tttttttic*sttrrhck*rt*ca---

---xxxxxxxxxxxxxxxxxxnxcxrl*cxxxxxh*sxss**xxydfixx*sws*scqsk
sk*n***pshx*ecrch*gcscreskctx*kipnlrfl*eck*ydlsasflfnessrrk*
ysftnrk*sf

Frame B:
iktqnnthlhniftilnyi*irqqq*q*qqqpil*YTYKYKIMNPDFTVYVSNISLKANT
KTVSDFFSFCGRIVNLFLRNDPTGSSQQAIVVFESDSAAKTALLLTNALIVDKVIQVVQF
TPELEFDLTQQFSQQQQFNNLSGEQQPQQQQYVSQPQEDIVNREHSVP---

---XXXXXXXNXXSXXXXXXSIXAAGYSVXQXXAIKARQVDEXHMISLXLKVGAEAVKAK
ANEIDNNLHIXESAAAIKGAVVERANALDERFQISGFFKSASDMISQXASYLMKAAEENS
TVSPIVNKV

Frame C:
*klktthtytiyllf*iisksdnnnnnnnnnpffnihinik**iqisqfmfqifh*kqil
kqfqifshfveelliyf*emtqlvvhnkq**ylnqiqlqkqlyy*qmh*l*ik*fklfnl
hqn*sli*hnnflnnnnliisvvnnnhnnnnmlvnhkktl*ienivc---

---xxxxxxxxxxvxxxxxxqxxxqvivlxkxxplkxvklmkxi*fhxxlklelklskqk
qmkliitftfxrvplplrvql*reqmhxmkdsksqvslrvqvi*slsxlpi**kqpkkiv
qfhqs*ikf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO778 (VFO778Q) /CSM/VF/VFO7-D/VFO778Q.Seq.d/                        571   e-162
VFO676 (VFO676Q) /CSM/VF/VFO6-D/VFO676Q.Seq.d/                        571   e-162
VFO336 (VFO336Q) /CSM/VF/VFO3-B/VFO336Q.Seq.d/                        571   e-162
VFL607 (VFL607Q) /CSM/VF/VFL6-A/VFL607Q.Seq.d/                        571   e-162
VFJ755 (VFJ755Q) /CSM/VF/VFJ7-C/VFJ755Q.Seq.d/                        571   e-162
VFI288 (VFI288Q) /CSM/VF/VFI2-D/VFI288Q.Seq.d/                        571   e-162
VFG496 (VFG496Q) /CSM/VF/VFG4-D/VFG496Q.Seq.d/                        571   e-162
VFG273 (VFG273Q) /CSM/VF/VFG2-D/VFG273Q.Seq.d/                        571   e-162
VFE514 (VFE514Q) /CSM/VF/VFE5-A/VFE514Q.Seq.d/                        571   e-162
VFE152 (VFE152Q) /CSM/VF/VFE1-C/VFE152Q.Seq.d/                        571   e-162
own update 2001.12. 2
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL844506|AL844506.1 Plasmodium falciparum chromosome  7.           40   2e-05  17
AE014829|AE014829.1 Plasmodium falciparum 3D7 chromosome 10 section 1 of 7 of the complete sequence.    46   9e-05  11
AE014845|AE014845.1 Plasmodium falciparum 3D7 chromosome 12, section 2 of 9 of the complete sequence.    30   2e-04  15
AE014820|AE014820.1 Plasmodium falciparum 3D7 chromosome 14 section 5 of 13 of the complete sequence.    36   3e-04  10
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    32   6e-04  17
AC005139|AC005139.3 Plasmodium falciparum chromosome 12, WORKING DRAFT SEQUENCE, 5 unordered pieces.    38   7e-04  12
BX842644|BX842644.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 3/4.    34   0.001  12
AE014824|AE014824.1 Plasmodium falciparum 3D7 chromosome 14 section 9 of 13 of the complete sequence.    34   0.001  13
AC005308|AC005308.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces.    30   0.001  13
AL929355|AL929355.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 1/5.    34   0.002  14
dna update 2004. 8.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR954203_362(CR954203|pid:none) Ostreococcus tauri strain OTTH05...    44   0.005
AP007171_537(AP007171|pid:none) Aspergillus oryzae RIB40 genomic...    42   0.020
AM920437_2007(AM920437|pid:none) Penicillium chrysogenum Wiscons...    41   0.045
EU958085_1(EU958085|pid:none) Zea mays clone 1672545 RNA-binding...    41   0.059
AC091811_13(AC091811|pid:none) Oryza sativa chromosome 3 BAC OSJ...    40   0.13
BT068289_1(BT068289|pid:none) Zea mays full-length cDNA clone ZM...    39   0.17
BT061154_1(BT061154|pid:none) Zea mays full-length cDNA clone ZM...    39   0.17
AC150647_11(AC150647|pid:none) Medicago truncatula clone mth2-65...    39   0.22
AC090882_24(AC090882|pid:none) Oryza sativa chromosome 3 BAC OSJ...    39   0.22
CR855144_15(CR855144|pid:none) Oryza sativa (indica cultivar-gro...    39   0.22
protein update 2009. 4.25
PSORT 

 psg: 0.84  gvh: 0.45  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: peroxisomal

>> prediction for AFN530 is cyt
5' end seq. ID AFN530F
5' end seq. 
>AFN530F.Seq
AATAAAAACTCAAAACAACACACACTTACACAATATATTTACTATTTTAAATTATATCTA
AATCCGACAACAACAATAACAATAACAACAACAACCCATTCTTTAATATACATATAAATA
TAAAATAATGAATCCAGATTTCACAGTTTATGTTTCAAATATTTCATTGAAAGCAAATAC
TAAAACAGTTTCAGATTTTTTCTCATTTTGTGGAAGAATTGTTAATTTATTTTTAAGAAA
TGACCCAACTGGTAGTTCACAACAAGCAATAGTAGTATTTGAATCAGATTCAGCTGCAAA
AACAGCTTTATTATTAACAAATGCATTAATTGTAGATAAAGTAATTCAAGTTGTTCAATT
TACACCAGAATTAGAGTTTGATTTAACACAACAATTTTCTCAACAACAACAATTTAATAA
TCTCAGTGGTGAACAACAACCACAACAACAACAATATGTTAGTCAACCACAAGAAGACAT
TGTAAATAGAGAACATAGTGTGCCA----------
Length of 5' end seq. 505
3' end seq. ID AFN530Z
3' end seq. 
>AFN530Z.Seq
----------TANNGNNNNTANTGNNNNNNNNAATNAANGNAGTNNNNCNTNNGNTGNTG
NNTCAATTNNTGCNGCAGGTTATAGTGTTGNNCAAGNNGNNGCCATTAAAGCNCGTCAAG
TTGATGAAGNNCATATGATTTCATTANNNCTTAAAGTTGGAGCTGAAGCTGTCAAAGCAA
AAGCAAATGAAATTGATAATAACCTTCACATTTNTGAGAGTGCCGCTGCCATTAAGGGTG
CAGTTGTAGAGAGAGCAAATGCACTNGATGAAAGATTCCAAATCTCAGGTTTCTTTAAGA
GTGCAAGTGATATGATCTCTCAGCNAGCTTCCTATTTAATGAAAGCAGCCGAAGAAAATA
GTACAGTTTCACCAATCGTAAATAAAGTTTC
Length of 3' end seq. 381
Connected seq. ID AFN530P
Connected seq. 
>AFN530P.Seq
AATAAAAACTCAAAACAACACACACTTACACAATATATTTACTATTTTAAATTATATCTA
AATCCGACAACAACAATAACAATAACAACAACAACCCATTCTTTAATATACATATAAATA
TAAAATAATGAATCCAGATTTCACAGTTTATGTTTCAAATATTTCATTGAAAGCAAATAC
TAAAACAGTTTCAGATTTTTTCTCATTTTGTGGAAGAATTGTTAATTTATTTTTAAGAAA
TGACCCAACTGGTAGTTCACAACAAGCAATAGTAGTATTTGAATCAGATTCAGCTGCAAA
AACAGCTTTATTATTAACAAATGCATTAATTGTAGATAAAGTAATTCAAGTTGTTCAATT
TACACCAGAATTAGAGTTTGATTTAACACAACAATTTTCTCAACAACAACAATTTAATAA
TCTCAGTGGTGAACAACAACCACAACAACAACAATATGTTAGTCAACCACAAGAAGACAT
TGTAAATAGAGAACATAGTGTGCCA----------TANNGNNNNTANTGNNNNNNNNAAT
NAANGNAGTNNNNCNTNNGNTGNTGNNTCAATTNNTGCNGCAGGTTATAGTGTTGNNCAA
GNNGNNGCCATTAAAGCNCGTCAAGTTGATGAAGNNCATATGATTTCATTANNNCTTAAA
GTTGGAGCTGAAGCTGTCAAAGCAAAAGCAAATGAAATTGATAATAACCTTCACATTTNT
GAGAGTGCCGCTGCCATTAAGGGTGCAGTTGTAGAGAGAGCAAATGCACTNGATGAAAGA
TTCCAAATCTCAGGTTTCTTTAAGAGTGCAAGTGATATGATCTCTCAGCNAGCTTCCTAT
TTAATGAAAGCAGCCGAAGAAAATAGTACAGTTTCACCAATCGTAAATAAAGTTTC
Length of connected seq. 886
Full length Seq ID -
Full length Seq. -
Length of full length seq. -