>VHC182 (VHC182Q) /CSM/VH/VHC1-D/VHC182Q.Seq.d/
ATATAAATAATAATATAGTTGAAAAGATGTGTGATCATAAACATTTAGTTGACAAAGTAG
ATAGATTAGTATTACCAGATAATATCGTACCAAGTAAATACGATTTACATCTTAGACCAA
ATTTAAAGGATTTTGTATTCAGCGGCAAAGTTGACATTACAATCAATATTGTTAAACCAA
CCAAAAAAATCATTATCCATTCAATTGACATTGAAATTCAATCAGTAAAGATCTTAAATC
AACAAGCTACTTCCGTTACCTACTATGAACCAGAAGAAGTTGCAATTTTAGAATTCCAAG
ATGAATTACCAGTTACAGAAAATACAATTCTCTCCATTGATTTCACTGGTATTTTAAATG
ACAAATTAAAAGGTTTCTATCGTTCAAAATATGTTGTCAATGGTGAAGATCGTTATATTG
GTACCACTCAATTCGAAGCCACTGATGCTCGTCGTGCTTTCCCATGTTTTGATGAACCTG
CTTTAAAATCTTTCTTTAATATTAAAATCACTATTTCATCTCATTTAACTGCTCTTTCAA
ATATGGATACAACTTCTGTAATTGAAAATAATGATGGTACTAAAACCTTTATTTTTGAAC
AAACTCCAAAGATGAGTACTTATATTXXXXXXXXXXTACAATCGTAAAGAATGGTGGTGA
AGCTGATCAACAAATCATCATTGATCAATATCTTAAATTTTCTTTAGTCGCTGAAAAGAA
TTCTTACCTTTTGGNACTTGCTTTGGCTCCAAAANAGGAATTGGTTGAAAAAGCCCTCAA
TTTCGCTTTATNTCCATCCGTTAGAAGTCNAGATAGTTATATGGTTTTCTTTACCCTCCC
AAATCGTGTTCGTCAATTCGCTTGGGCTTACCTCNCCAAAAACTTTAATCAAATCAACGA
AACATTTAAAAACTNTCCTTTGTTTGGTCGTATCATTGGTAGTTGTNTCNCCTNTAAAAT
GGATGATTNTCAATACAATGAAGTCGTCAACTTTTTCAAAGATCATCCAGTTCCAATTGC
TGATCGTTCAATTAAACAAGATTTAGAAANGGTTCCAATTAATTCAAATTGGTTCAAAGC
TTTTAATCAAGATTTATCAAATTGGATTCAATCAAAATAAAAATAAAATNTNTTCCAATG
AAAGNTCTNTGTTTTAATAATTGCATAGNGTATAAAAAAAAAATGAGACGTANAACTAAA

INNNIVEKMCDHKHLVDKVDRLVLPDNIVPSKYDLHLRPNLKDFVFSGKVDITINIVKPT
KKIIIHSIDIEIQSVKILNQQATSVTYYEPEEVAILEFQDELPVTENTILSIDFTGILND
KLKGFYRSKYVVNGEDRYIGTTQFEATDARRAFPCFDEPALKSFFNIKITISSHLTALSN
MDTTSVIENNDGTKTFIFEQTPKMSTYI---

---TIVKNGGEADQQIIIDQYLKFSLVAEKNSYLLXLALAPKXELVEKALNFALXPSVRS
XDSYMVFFTLPNRVRQFAWAYLXKNFNQINETFKNXPLFGRIIGSCXXXKMDDXQYNEVV
NFFKDHPVPIADRSIKQDLEXVPINSNWFKAFNQDLSNWIQSK*k*nxfq*kxxvliia*
xikkk*dvxl

Frame A:
i*iii*lkrcviini*ltk*id*yyqiisyqvntiyildqi*rilysaakltlqsillnq
pkkslsiqltlkfnq*rs*inkllplptmnqkklqf*nskmnyqlqkiqfsplislvf*m
tn*kvsivqnmlsmvkivilvplnskplmlvvlshvlmnll*nlslilkslfhli*llfq
iwiqll*lkimmvlkplflnklqr*vli---

---ynrkeww*s*stnhh*sis*iffsr*keflpfgtcfgskxgig*kspqfrfxsir*k
sr*lygflyppkscssirlglpxqkl*snqrni*klsfvwsyhw*lxxl*ng*xsiq*sr
qlfqrssssnc*sfn*trfrxgsn*fklvqsf*srfikldsikikikxxpmkxlcfnnci
xykkkmrrxtk

Frame B:
yk**ys*kdv*s*tfs*qsr*isitr*yrtk*irfts*tkfkgfciqrqs*hynqyc*tn
qknhypfn*h*nsiskdlkstsyfryll*trrscnfripr*itsyrkynslh*fhwyfk*
qikrflsfkiccqw*rslywyhsirsh*csscfpmf**tcfkifl*y*nhyfisfncsfk
ygynfcn*k**wy*nlyf*tnskdeyly---

---TIVKNGGEADQQIIIDQYLKFSLVAEKNSYLLXLALAPKXELVEKALNFALXPSVRS
XDSYMVFFTLPNRVRQFAWAYLXKNFNQINETFKNXPLFGRIIGSCXXXKMDDXQYNEVV
NFFKDHPVPIADRSIKQDLEXVPINSNWFKAFNQDLSNWIQSK*k*nxfq*kxxvliia*
xikkk*dvxl

Frame C:
INNNIVEKMCDHKHLVDKVDRLVLPDNIVPSKYDLHLRPNLKDFVFSGKVDITINIVKPT
KKIIIHSIDIEIQSVKILNQQATSVTYYEPEEVAILEFQDELPVTENTILSIDFTGILND
KLKGFYRSKYVVNGEDRYIGTTQFEATDARRAFPCFDEPALKSFFNIKITISSHLTALSN
MDTTSVIENNDGTKTFIFEQTPKMSTYI---

---qs*rmvvklinksslinilnfl*slkriltfwxllwlqxrnwlkkpsislyxhplev
xiviwfslpsqivfvnslgltspktlikstkhlktxlclvvslvvvspxkwmixntmkss
tfskiiqfqllivqlnki*kxfqliqigskllikiyqigfnqnknkxxsnexsxf**lhx
v*kknetxn*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC182 (VHC182Q) /CSM/VH/VHC1-D/VHC182Q.Seq.d/                       2129   0.0
VHQ308 (VHQ308Q) /CSM/VH/VHQ3-A/VHQ308Q.Seq.d/                       1191   0.0
VHP808 (VHP808Q) /CSM/VH/VHP8-A/VHP808Q.Seq.d/                       1191   0.0
VHP634 (VHP634Q) /CSM/VH/VHP6-B/VHP634Q.Seq.d/                       1191   0.0
VHP126 (VHP126Q) /CSM/VH/VHP1-B/VHP126Q.Seq.d/                       1191   0.0
VHN323 (VHN323Q) /CSM/VH/VHN3-A/VHN323Q.Seq.d/                       1191   0.0
VHN118 (VHN118Q) /CSM/VH/VHN1-A/VHN118Q.Seq.d/                       1191   0.0
VHM664 (VHM664Q) /CSM/VH/VHM6-C/VHM664Q.Seq.d/                       1191   0.0
VHM140 (VHM140Q) /CSM/VH/VHM1-B/VHM140Q.Seq.d/                       1191   0.0
VHK649 (VHK649Q) /CSM/VH/VHK6-C/VHK649Q.Seq.d/                       1191   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY631393|AY631393.1 Dictyostelium discoideum puromycin-sensitive aminopeptidase-like protein (psa) mRNA, partial cds.    80   7e-13  3
CR382136|CR382136.1 Debaryomyces hansenii chromosome D of strain CBS767 of Debaryomyces hansenii.    56   0.002  1
CP000498|CP000498.1 Pichia stipitis CBS 6054 chromosome 4, complete sequence.    38   0.006  2
CR382123|CR382123.1 Kluyveromyces lactis strain NRRL Y-1140 chromosome C of strain NRRL  Y-1140 of Kluyveromyces lactis.    54   0.009  1
DN815134|AF148323_1 aminopeptidase PILS [Rattus norvegicus], mRNA sequence.    52   0.037  1
BP514490|BP514490.1 Hydra magnipapillata cDNA, clone:hydmg018bw_14.    52   0.037  1
DQ229107|DQ229107.1 Chara vulgaris chloroplast, complete genome.    46   0.11   9
AJ808281|AJ808281.1 Antirrhinum majus EST, clone 018_6_09_c24.     50   0.15   1
CB564189|CB564189.1 AGENCOURT_13329805 NICHD_XGC_Tad2 Xenopus laevis cDNA clone IMAGE:6875222 3', mRNA sequence.    48   0.58   1
CL085130|CL085130.1 ISB1-4I13_T7.1 ISB1 Xenopus tropicalis genomic clone ISB1-4I13, genomic survey sequence.    48   0.58   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF327435_1(AF327435|pid:none) Drosophila melanogaster puromycin-...   162   2e-38
AE014296_282(AE014296|pid:none) Drosophila melanogaster chromoso...   162   2e-38
AE014296_281(AE014296|pid:none) Drosophila melanogaster chromoso...   162   2e-38
AM270075_67(AM270075|pid:none) Aspergillus niger contig An04c014...   145   2e-33
AP007157_574(AP007157|pid:none) Aspergillus oryzae RIB40 genomic...   143   1e-32
EF678434_1(EF678434|pid:none) Picea sitchensis clone WS02921_A14...   140   9e-32
(P32454) RecName: Full=Aminopeptidase 2, mitochondrial;         ...   139   2e-31
S37794(S37794;S37987;S44571;S19642) aminopeptidase yscII (EC 3.4...   139   2e-31
EF676179_1(EF676179|pid:none) Picea sitchensis clone WS02717_G19...   139   2e-31
AF350242_1(AF350242|pid:none) Pichia angusta aminopeptidase gene...   139   2e-31

 psg: 0.75  gvh: 0.34  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal

>> prediction for VHC182 is cyt

>VHC182F.Seq
ATATAAATAATAATATAGTTGAAAAGATGTGTGATCATAAACATTTAGTTGACAAAGTAG
ATAGATTAGTATTACCAGATAATATCGTACCAAGTAAATACGATTTACATCTTAGACCAA
ATTTAAAGGATTTTGTATTCAGCGGCAAAGTTGACATTACAATCAATATTGTTAAACCAA
CCAAAAAAATCATTATCCATTCAATTGACATTGAAATTCAATCAGTAAAGATCTTAAATC
AACAAGCTACTTCCGTTACCTACTATGAACCAGAAGAAGTTGCAATTTTAGAATTCCAAG
ATGAATTACCAGTTACAGAAAATACAATTCTCTCCATTGATTTCACTGGTATTTTAAATG
ACAAATTAAAAGGTTTCTATCGTTCAAAATATGTTGTCAATGGTGAAGATCGTTATATTG
GTACCACTCAATTCGAAGCCACTGATGCTCGTCGTGCTTTCCCATGTTTTGATGAACCTG
CTTTAAAATCTTTCTTTAATATTAAAATCACTATTTCATCTCATTTAACTGCTCTTTCAA
ATATGGATACAACTTCTGTAATTGAAAATAATGATGGTACTAAAACCTTTATTTTTGAAC
AAACTCCAAAGATGAGTACTTATATTNNNNNNNNNN

>VHC182Z.Seq
NNNNNNNNNNTACAATCGTAAAGAATGGTGGTGAAGCTGATCAACAAATCATCATTGATC
AATATCTTAAATTTTCTTTAGTCGCTGAAAAGAATTCTTACCTTTTGGNACTTGCTTTGG
CTCCAAAANAGGAATTGGTTGAAAAAGCCCTCAATTTCGCTTTATNTCCATCCGTTAGAA
GTCNAGATAGTTATATGGTTTTCTTTACCCTCCCAAATCGTGTTCGTCAATTCGCTTGGG
CTTACCTCNCCAAAAACTTTAATCAAATCAACGAAACATTTAAAAACTNTCCTTTGTTTG
GTCGTATCATTGGTAGTTGTNTCNCCTNTAAAATGGATGATTNTCAATACAATGAAGTCG
TCAACTTTTTCAAAGATCATCCAGTTCCAATTGCTGATCGTTCAATTAAACAAGATTTAG
AAANGGTTCCAATTAATTCAAATTGGTTCAAAGCTTTTAATCAAGATTTATCAAATTGGA
TTCAATCAAAATAAAAATAAAATNTNTTCCAATGAAAGNTCTNTGTTTTAATAATTGCAT
AGNGTATAAAAAAAAAATGAGACGTANAACTAAA

>VHC182P.Seq
ATATAAATAATAATATAGTTGAAAAGATGTGTGATCATAAACATTTAGTTGACAAAGTAG
ATAGATTAGTATTACCAGATAATATCGTACCAAGTAAATACGATTTACATCTTAGACCAA
ATTTAAAGGATTTTGTATTCAGCGGCAAAGTTGACATTACAATCAATATTGTTAAACCAA
CCAAAAAAATCATTATCCATTCAATTGACATTGAAATTCAATCAGTAAAGATCTTAAATC
AACAAGCTACTTCCGTTACCTACTATGAACCAGAAGAAGTTGCAATTTTAGAATTCCAAG
ATGAATTACCAGTTACAGAAAATACAATTCTCTCCATTGATTTCACTGGTATTTTAAATG
ACAAATTAAAAGGTTTCTATCGTTCAAAATATGTTGTCAATGGTGAAGATCGTTATATTG
GTACCACTCAATTCGAAGCCACTGATGCTCGTCGTGCTTTCCCATGTTTTGATGAACCTG
CTTTAAAATCTTTCTTTAATATTAAAATCACTATTTCATCTCATTTAACTGCTCTTTCAA
ATATGGATACAACTTCTGTAATTGAAAATAATGATGGTACTAAAACCTTTATTTTTGAAC
AAACTCCAAAGATGAGTACTTATATT----------TACAATCGTAAAGAATGGTGGTGA
AGCTGATCAACAAATCATCATTGATCAATATCTTAAATTTTCTTTAGTCGCTGAAAAGAA
TTCTTACCTTTTGGNACTTGCTTTGGCTCCAAAANAGGAATTGGTTGAAAAAGCCCTCAA
TTTCGCTTTATNTCCATCCGTTAGAAGTCNAGATAGTTATATGGTTTTCTTTACCCTCCC
AAATCGTGTTCGTCAATTCGCTTGGGCTTACCTCNCCAAAAACTTTAATCAAATCAACGA
AACATTTAAAAACTNTCCTTTGTTTGGTCGTATCATTGGTAGTTGTNTCNCCTNTAAAAT
GGATGATTNTCAATACAATGAAGTCGTCAACTTTTTCAAAGATCATCCAGTTCCAATTGC
TGATCGTTCAATTAAACAAGATTTAGAAANGGTTCCAATTAATTCAAATTGGTTCAAAGC
TTTTAATCAAGATTTATCAAATTGGATTCAATCAAAATAAAAATAAAATNTNTTCCAATG
AAAGNTCTNTGTTTTAATAATTGCATAGNGTATAAAAAAAAAATGAGACGTANAACTAAA