>VHC161 (VHC161Q) /CSM/VH/VHC1-C/VHC161Q.Seq.d/
GGATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAACTAAACTTAATCAA
TTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCATTAGATGAAACATTA
AATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAATAAACCTCATGAACAA
TTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAAAGTAATATTAGTA
TTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGAAAGTAAATTTTAT
TCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGGTGATGTACAAATT
GAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTTGTAAATAGATGTTAT
TCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAATCACAAAAGAATATA
CATACACAATTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTCAATGATTGATATA
TTTTCAGTATTAATAAATTAGATAGTATAATTACACAAAATACTGCTTTAGATTCATCTT
GGGGTAXXXXXXXXXXCAATTGGGTGTCTCCGAGACTGGTTTACCNTTTTTAGATCATTT
TAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCAGATCCGG
TGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAA
ATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAA
TTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACTTTAA
GATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAA
TTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCAATAGTAA
AGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTGATGA
TGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATTTCGA
TTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAAAGAGAATGTT
GGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTAAAAAGAT
TCAAAATTTCCAACAAGAATATGAATTATTTAAATATTCTTTCTCGGTTCAAGAATTTTC
T

i*rwfi*igwratin*t*sics*lcttnledrrsir*nik*smgykyrssfnl**ts*ti
rfn*ig*n*s*nfq*snisilfnl*sn*nfkrnsrk*ilftinsiwrdyr*iirr*ctn*
sw*iitiyd*fisick*mlfin*kyy*sicidlsitkeytytil*ecsftscllfnd*YI
FSINKLDSIITQNTALDSSWG---

---QLGVSETGLPFLDHFRLLITHIGNAMGYIRLVRSGGLHYCSNAIKFVPDLKKIPKFQ
DLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEYFKMLVNVFATEFRNIANQHLKNFY
IIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKLLDQNKDFDSL
HWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIKKIQNFQQEYELFKYSFSVQEFS

Frame A:
gfedgssklvgeqqltklnqfavdyaqqiwkieealdetlnevwdinidpvsiynkpheq
ldlielvktdhkifnkvilvfssicnqtrilketaeskfyspltvfgeitgessegdvqi
evgkllpfmidlsafvnrcyslirniisqfasiyqsqknihtqffknvhlqavyysmidi
fsvlin*iv*lhkill*ihlgv---

---QLGVSETGLPFLDHFRLLITHIGNAMGYIRLVRSGGLHYCSNAIKFVPDLKKIPKFQ
DLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEYFKMLVNVFATEFRNIANQHLKNFY
IIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKLLDQNKDFDSL
HWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIKKIQNFQQEYELFKYSFSVQEFS

Frame B:
dlkmvhlnwlesnn*lnlinlqlimhnkfgr*kkh*mkh*mkygi*i*iqfqfiinlmnn
*i*lnwlkliikfsik*y*yslqfviklef*kkqqkvnfihh*qylerlqvnhqkvmykl
klvnyyhl*liyqhl*idvih*leillvnlhrsinhkriyihnslrmfiyklsiiq*liy
fqy**ir*ynytkycfrfilg---

---nwvsprlvyxf*iildf*slilvmlwvildwsdpvvytivvmlsnsyqt*krfqnsk
i*pqkmpshlkqfklvpiwiqsfiifqiiyqrvlntlrcw*tfslqsfeislini*risi
ssylpwpqitsii*sivkinslrnqkqsvlklyyslmmalqlvwlis*ny*ikikisilf
igsivfn*nvklikrecwlkqmqkvskrikii*qlkrfkisnknmnylnilsrfknf

Frame C:
i*rwfi*igwratin*t*sics*lcttnledrrsir*nik*smgykyrssfnl**ts*ti
rfn*ig*n*s*nfq*snisilfnl*sn*nfkrnsrk*ilftinsiwrdyr*iirr*ctn*
sw*iitiyd*fisick*mlfin*kyy*sicidlsitkeytytil*ecsftscllfnd*YI
FSINKLDSIITQNTALDSSWG---

---igclrdwftxfrsf*tfnhsyw*cygly*igqirwftll**cyqirtrlekdskipr
fnlkrcplt*nnss*yqfgfnhs*sfk*firgy*il*dvgkrfryrvskyr*stfkefly
hrtclglklhrsydq**r*tl*eiksnrc*sfiih**wlcnwfglylkiirsk*rfrfss
lvrsysiem*n*skenvg*skckryqrgsksfsn*kdskfptri*ii*ifflgsrif

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC161 (VHC161Q) /CSM/VH/VHC1-C/VHC161Q.Seq.d/                       2553   0.0
AHC187 (AHC187Q) /CSM/AH/AHC1-D/AHC187Q.Seq.d/                       1372   0.0
VHK538 (VHK538Q) /CSM/VH/VHK5-B/VHK538Q.Seq.d/                       1364   0.0
CHG465 (CHG465Q) /CSM/CH/CHG4-C/CHG465Q.Seq.d/                       1364   0.0
AHL641 (AHL641Q) /CSM/AH/AHL6-B/AHL641Q.Seq.d/                       1364   0.0
VHK424 (VHK424Q) /CSM/VH/VHK4-A/VHK424Q.Seq.d/                       1362   0.0
VHI518 (VHI518Q) /CSM/VH/VHI5-A/VHI518Q.Seq.d/                       1362   0.0
VHA684 (VHA684Q) /CSM/VH/VHA6-D/VHA684Q.Seq.d/                       1362   0.0
CHE650 (CHE650Q) /CSM/CH/CHE6-C/CHE650Q.Seq.d/                       1362   0.0
VHB619 (VHB619Q) /CSM/VH/VHB6-A/VHB619Q.Seq.d/                       1358   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    40   1e-04  13
BU746157|BU746157.1 CH3#002_C07T7 Canine heart normalized cDNA Library in pBluescript Canis familiaris cDNA clone CH3#002_C07 5', mRNA sequence.    38   8e-04  3
CP000123|CP000123.1 Mycoplasma capricolum subsp. capricolum ATCC 27343, complete genome.    42   0.003  20
BY464171|BY464171.1 Mus musculus 15 days pregnant adult female amnion cDNA, RIKEN full-length enriched library, clone:K630150M24, 3' end partial sequence.    42   0.034  2
AE017308|AE017308.1 Mycoplasma mobile 163K complete genome.        36   0.064  18
BB822673|BB822673.1 Mus musculus mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830022O04, 3' end partial sequence.    32   0.12   4
AK113047|AK113047.1 Ciona intestinalis cDNA, clone:ciad002a21, full insert sequence.    48   0.14   2
DN287505|DN287505.1 1241755 MARC 7BOV Bos taurus cDNA 3', mRNA sequence.    34   0.14   3
AC100352|AC100352.1 Mus musculus clone RP23-127M10, LOW-PASS SEQUENCE SAMPLING.    50   0.16   1
AG495365|AG495365.1 Mus musculus molossinus DNA, clone:MSMg01-394E07.T7, genomic survey sequence.    50   0.16   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54R74) RecName: Full=UPF0681 protein;                               458   e-127
BC040936_1(BC040936|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   231   3e-59
AB028956_1(AB028956|pid:none) Homo sapiens mRNA for KIAA1033 pro...   231   4e-59
BC143373_1(BC143373|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   231   4e-59
BC020314_1(BC020314|pid:none) Mus musculus RIKEN cDNA A230046K03...   229   2e-58
(Q3UMB9) RecName: Full=UPF0681 protein KIAA1033;                      229   2e-58
AK161247_1(AK161247|pid:none) Mus musculus adult male testis cDN...   229   2e-58
AK145000_1(AK145000|pid:none) Mus musculus mammary gland RCB-052...   229   2e-58
BC110850_1(BC110850|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   168   5e-40
AX882617_1(AX882617|pid:none) Sequence 17522 from Patent EP10746...   163   1e-38

 psg: 0.80  gvh: 0.51  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VHC161 is cyt

>VHC161F.Seq
GGATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAACTAAACTTAATCAA
TTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCATTAGATGAAACATTA
AATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAATAAACCTCATGAACAA
TTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAAAGTAATATTAGTA
TTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGAAAGTAAATTTTAT
TCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGGTGATGTACAAATT
GAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTTGTAAATAGATGTTAT
TCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAATCACAAAAGAATATA
CATACACAATTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTCAATGATTGATATA
TTTTCAGTATTAATAAATTAGATAGTATAATTACACAAAATACTGCTTTAGATTCATCTT
GGGGTANNNNNNNNNN

>VHC161Z.Seq
NNNNNNNNNNCAATTGGGTGTCTCCGAGACTGGTTTACCNTTTTTAGATCATTTTAGACT
TTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCAGATCCGGTGGTTT
ACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAATTCCA
AGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAATTTGGA
TTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACTTTAAGATGTT
GGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAATTTCTA
TATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCAATAGTAAAGATAA
ACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTGATGATGGCTT
TGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATTTCGATTCTCT
TCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAAAGAGAATGTTGGCTGA
AGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTAAAAAGATTCAAAA
TTTCCAACAAGAATATGAATTATTTAAATATTCTTTCTCGGTTCAAGAATTTTCT

>VHC161P.Seq
GGATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAACTAAACTTAATCAA
TTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCATTAGATGAAACATTA
AATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAATAAACCTCATGAACAA
TTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAAAGTAATATTAGTA
TTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGAAAGTAAATTTTAT
TCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGGTGATGTACAAATT
GAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTTGTAAATAGATGTTAT
TCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAATCACAAAAGAATATA
CATACACAATTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTCAATGATTGATATA
TTTTCAGTATTAATAAATTAGATAGTATAATTACACAAAATACTGCTTTAGATTCATCTT
GGGGTA----------CAATTGGGTGTCTCCGAGACTGGTTTACCNTTTTTAGATCATTT
TAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCAGATCCGG
TGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAA
ATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAA
TTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACTTTAA
GATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAA
TTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCAATAGTAA
AGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTGATGA
TGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATTTCGA
TTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAAAGAGAATGTT
GGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTAAAAAGAT
TCAAAATTTCCAACAAGAATATGAATTATTTAAATATTCTTTCTCGGTTCAAGAATTTTC
T