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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHC160
Library VH
(Link to library)
Clone ID VHC160
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHC160P
(Link to Original site)
Representative DNA sequence 
>VHC160 (VHC160Q) /CSM/VH/VHC1-C/VHC160Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAANGAATTCGGTGATGTCACTGTATCCXXXXXXXXXXCCAGAAGGTAACGGT
GCCAATCTCNTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCT
TTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGA
GGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGT
GGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCA
ACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGT
ATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGT
TCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCT
GATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCT
TCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAG
AGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACA
ATTTAAGTCGCACTTTNAGTTTTCAAAGATAGTAATCAAATCTAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDXEFGDVTVS---

---PEGNGANLXVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITAPENAIGGIYSVLNRRRGIV
IGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRKRKGLAPEIPDLDKFHEKL*ti*valxvfkdsnqi*kk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlkixnsvmsly---

---PEGNGANLXVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITAPENAIGGIYSVLNRRRGIV
IGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRKRKGLAPEIPDLDKFHEKL*ti*valxvfkdsnqi*kk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r*xir*chci---

---qkvtvpisssmspkvfni*mklkilslvlsngppkkvlfvmkt*evsvsifmmspst
lmpstevvvkssqllvvysmlln*lppqls*nqst**kllpqktllvvftlysivvevll
lvknvvsvphyslskliyqy*npsvspliyvptlpvklshnvssiiglqlvlstkirkpl
ksl*pperekv*pqksqiltnstknskqfkshfxfskivikskk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDXEFGDVTVS---

---rr*rcqsxrqchqrcsife*n*rffrwcfpmghqrrccl**kherypfqsl*chpph
*chpqrwwsnhpncssctlcc*inclpnslrtnllsrnycprkrywwyllctqss*rycy
w*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh*
srfshqkekrfsprnprs*qiprktlnnlsrtxsfqr**snlkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC160 (VHC160Q) /CSM/VH/VHC1-C/VHC160Q.Seq.d/                       2466   0.0
VHQ446 (VHQ446Q) /CSM/VH/VHQ4-B/VHQ446Q.Seq.d/                       1287   0.0
VHQ318 (VHQ318Q) /CSM/VH/VHQ3-A/VHQ318Q.Seq.d/                       1287   0.0
VHQ252 (VHQ252Q) /CSM/VH/VHQ2-C/VHQ252Q.Seq.d/                       1287   0.0
VHP769 (VHP769Q) /CSM/VH/VHP7-C/VHP769Q.Seq.d/                       1287   0.0
VHP749 (VHP749Q) /CSM/VH/VHP7-C/VHP749Q.Seq.d/                       1287   0.0
VHK310 (VHK310Q) /CSM/VH/VHK3-A/VHK310Q.Seq.d/                       1287   0.0
VHJ682 (VHJ682Q) /CSM/VH/VHJ6-D/VHJ682Q.Seq.d/                       1287   0.0
VHI434 (VHI434Q) /CSM/VH/VHI4-B/VHI434Q.Seq.d/                       1287   0.0
VHI384 (VHI384Q) /CSM/VH/VHI3-D/VHI384Q.Seq.d/                       1287   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1211   0.0    1
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   1e-65  8
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   2e-64  8
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    72   5e-64  12
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    70   1e-58  9
AF534908|AF534908.2 Tetrahymena thermophila elongation factor 2 gene, complete cds.    84   2e-45  5
EH015431|EH015431.1 USDA-FP_188032 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt081_G08 5', mRNA sequence.    78   6e-43  4
EH012247|EH012247.1 USDA-FP_185167 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt044_H07 5', mRNA sequence.    78   8e-43  4
AF107290|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds.    64   2e-42  7
AY144999|AY144999.1 Saccharomyces kluyveri clone Contig1706 EFT2 gene, complete cds.    78   2e-40  6
dna update 2007. 4.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   270   6e-71
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   265   2e-69
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   265   3e-69
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   264   5e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   264   6e-69
DQ848932_1(DQ848932|pid:none) Scophthalmus maximus clone kbc101 ...   263   8e-69
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   263   1e-68
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   263   1e-68
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   263   1e-68
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   263   1e-68
protein update 2009. 6.28
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHC160 is cyt
5' end seq. ID VHC160F
5' end seq. 
>VHC160F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAANGAATTCGGTGATGTCACTGTATCCNNNNNNNNNN
Length of 5' end seq. 645
3' end seq. ID VHC160Z
3' end seq. 
>VHC160Z.Seq
NNNNNNNNNNCCAGAAGGTAACGGTGCCAATCTCNTCGTCAATGTCACCAAAGGTGTTCA
ATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGG
TGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCA
CACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTA
TGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTAC
TGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGT
TATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGT
ATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCC
ACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCAC
TGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGA
CAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTNAGTTTTCAAAGATAGTAA
TCAAATCTAAAAAAAA
Length of 3' end seq. 676
Connected seq. ID VHC160P
Connected seq. 
>VHC160P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAANGAATTCGGTGATGTCACTGTATCC----------CCAGAAGGTAACGGT
GCCAATCTCNTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCT
TTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGA
GGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGT
GGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCA
ACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGT
ATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGT
TCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCT
GATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCT
TCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAG
AGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACA
ATTTAAGTCGCACTTTNAGTTTTCAAAGATAGTAATCAAATCTAAAAAAAA
Length of connected seq. 1301
Full length Seq ID -
Full length Seq. -
Length of full length seq. -