>VHC152 (VHC152Q) /CSM/VH/VHC1-C/VHC152Q.Seq.d/
AGATTTGTCTTCTCAATTTTATTTTTCACCAGAACAAGTTGGTAAAGTTGGTAGAGCAGA
TGCATGTTTCCAAAAAGTTGTAGATTTAAATAATTATGTTAGAATTGATGTACATAATGG
AGAATTAAGTGATGAATTCTTAAAGAAATTCAATGTTGTAGTATTAGCCAATCAACCATT
AGCATTACAACTCAAAGTGAATGAATTTTGTCATGCAAATAAAATTCATTTCATTAGTGT
TGAAACCAGAGGTGTTTTTGGTCAATTATTCAATGATTTTGGTGAACAATTTACAATCAC
CGATACAAATGGTGAGAATCCAAATGCTTATATGATCAGTTCAATCAGTCAAGATAAAGA
AGGTATTGTAACCGTTGTTGAAGAACAAAAATTACAACTTTTAGATGGTGATCTTGTTAC
ATTCAAAGAAGTCAATGGTATGTCAGCTCTTAATGATCTTCCACCACAAAAAATTAAAAC
CATCTCTCCATTAACTTTTTCAATTGGTGATACTACAAATCTTCCACCATATACTTCTGG
TGGTTATGTTACTGAAGTTAAACAACCAAAAGTTGTTGATTTTAAXXXXXXXXXXAACTC
TATTCAATTTGAAAAGGATGATGATACCAATCATCATATCGATTTCATTACCGCTACCTC
AAATTTAAGAGCTACCAACTATGCAATCAGTCCAGCTGATAAACATAAGACCAAAGGTAT
CGCCGGTAAAATCATTCCAGCTTTAGTTACCACTACTGCCGTAGTTGCTGGTTTCGTTTG
TATCGAATTGATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATATAAGAGCACCTTTAT
GAATTTAGGTATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGCCCCAAAGAACAAAAT
TCGTGAAGGCTGGACTTGGACACTTTGGGATCGTTTCGATGTTGATGGTGATATCACTCT
CAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATGGTTTGGATATTTCAATGCTCTCTTG
TAAAGTTACACTTTTATATGCTCTCTTCACTGATAAGAAAACTAAAGAGGAAAGATTAAA
GATGAAAATCTCACAACTCTATGAAACCCTCTCAAAGAAACCATTACCAAAAGATAAGAA
ATATTTATTACTGAAATTTGTTGCAAGATAC

DLSSQFYFSPEQVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEFLKKFNVVVLANQPL
ALQLKVNEFCHANKIHFISVETRGVFGQLFNDFGEQFTITDTNGENPNAYMISSISQDKE
GIVTVVEEQKLQLLDGDLVTFKEVNGMSALNDLPPQKIKTISPLTFSIGDTTNLPPYTSG
GYVTEVKQPKVVDF---

---NSIQFEKDDDTNHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTTAVV
AGFVCIELIKVIQNKALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRFDVD
GDITLKEFLDLFEKKHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSKKPL
PKDKKYLLLKFVARY

Frame A:
rfvfsilfftrtsw*sw*srcmfpkscrfk*lc*n*ct*wrik**ilkeiqccsisqsti
sittqse*ilsck*nsfh*c*nqrcfwsiiq*fw*tiynhrykw*eskclydqfnqsr*r
rycnrc*rtkittfrw*scyiqrsqwyvss**ssttkn*nhlsinffnw*yyksstiyfw
wlcy*s*ttksc*f*---

---NSIQFEKDDDTNHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTTAVV
AGFVCIELIKVIQNKALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRFDVD
GDITLKEFLDLFEKKHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSKKPL
PKDKKYLLLKFVARY

Frame B:
DLSSQFYFSPEQVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEFLKKFNVVVLANQPL
ALQLKVNEFCHANKIHFISVETRGVFGQLFNDFGEQFTITDTNGENPNAYMISSISQDKE
GIVTVVEEQKLQLLDGDLVTFKEVNGMSALNDLPPQKIKTISPLTFSIGDTTNLPPYTSG
GYVTEVKQPKVVDF---

---tlfnlkrmmipiiisislplpqi*elptmqsvqlinirpkvspvksfql*lpllp*l
lvsfvsn*lklfkiklwrnirapl*i*vshslvslnqlppqrtkfvkaglghfgivsmlm
vislsrsf*islkrnmvwifqcslvklhfymlsslirklkrkd*r*kshnsmkpsqrnhy
qkirniyy*nllqd

Frame C:
icllnfifhqnklvklveqmhvskkl*i*iimlelmyimen*vmns*rnsml*y*pinh*
hynsk*mnfvmqikfislvlkpevflvnysmilvnnlqspiqmvriqmli*svqsvkikk
vl*pllknknynf*mvillhskksmvcqllmifhhkklkpslh*lfqlvilqifhhillv
vmllklnnqkllil---

---lysi*kg**yqssyrfhyrylkfksyqlcnqss**t*dqryrr*nhssfsyhycrsc
wfrlyrid*sysk*sfgei*ehlyefrypilwfr*tncrpkeqns*rldldtlgsfrc*w
*yhsqgvfrsl*ketwfgyfnall*sytficslh**en*rgkikdenlttl*nplketit
kr*eifiteiccki

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC152 (VHC152Q) /CSM/VH/VHC1-C/VHC152Q.Seq.d/                       2262   0.0
SHI311 (SHI311Q) /CSM/SH/SHI3-A/SHI311Q.Seq.d/                       1146   0.0
AHK113 (AHK113Q) /CSM/AH/AHK1-A/AHK113Q.Seq.d/                       1140   0.0
VHP750 (VHP750Q) /CSM/VH/VHP7-C/VHP750Q.Seq.d/                       1132   0.0
VHP205 (VHP205Q) /CSM/VH/VHP2-A/VHP205Q.Seq.d/                       1132   0.0
VHN723 (VHN723Q) /CSM/VH/VHN7-A/VHN723Q.Seq.d/                       1132   0.0
VHC866 (VHC866Q) /CSM/VH/VHC8-C/VHC866Q.Seq.d/                       1132   0.0
VHC211 (VHC211Q) /CSM/VH/VHC2-A/VHC211Q.Seq.d/                       1132   0.0
VHB744 (VHB744Q) /CSM/VH/VHB7-B/VHB744Q.Seq.d/                       1132   0.0
VHA328 (VHA328Q) /CSM/VH/VHA3-B/VHA328Q.Seq.d/                       1132   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CB933531|CB933531.1 ri31b11.y1 Meloidogyne chitwoodi egg pAMP1 v1 Meloidogyne chitwoodi cDNA 5' similar to TR:Q27481 Q27481 C47E12.5 PROTEIN. [1] ;, mRNA sequence.    64   4e-12  2
AZ930776|AZ930776.1 474.dhz58e03.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz58e03.s1, DNA sequence.    68   1e-11  2
ED188983|ED188983.1 AUAC-aav62e08.g1 Ascaris suum whole genome shotgun library (PMAJ_4 GSS) Ascaris suum genomic, genomic survey sequence.    60   1e-04  1
AM562633|AM562633.1 Paracentrotus lividus EST, clone MPMGp1173D192Q, 5' end.    38   5e-04  2
DR174755|DR174755.1 HR0003_N01:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   6e-04  1
DR174741|DR174741.1 HR0003_M08:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   6e-04  1
DR174736|DR174736.1 HR0003_M02:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   6e-04  1
BZ303839|BZ303839.1 KD2618.q1 Kluyveromyces delphensis Random Genomic Library Kluyveromyces delphensis genomic clone KD2618, DNA sequence.    48   0.002  2
EE674138| ubiquitin activating enzyme [Drosophila melanogaster], mRNA sequence.    56   0.002  1
EE670055| similar to ubiquitin-activating enzyme E1, Chr X [Mus musculus] [Rattus norvegicus], mRNA sequence.    56   0.002  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55C16) RecName: Full=Ubiquitin-like modifier-activating enzyme...   393   e-108
EF468345_1(EF468345|pid:none) Pisum sativum ubiquitin activating...   213   8e-54
AE017341_129(AE017341|pid:none) Cryptococcus neoformans var. neo...   196   2e-48
AB035495_1(AB035495|pid:none) Carassius auratus mRNA for ubiquit...   195   2e-48
CR382138_755(CR382138|pid:none) Debaryomyces hansenii strain CBS...   194   4e-48
BC047256_1(BC047256|pid:none) Xenopus laevis ubiquitin-like modi...   193   1e-47
AB040073_1(AB040073|pid:none) Xenopus laevis E1 mRNA for ubiquit...   193   1e-47
CP000496_820(CP000496|pid:none) Pichia stipitis CBS 6054 chromos...   190   7e-47
BC064684_1(BC064684|pid:none) Xenopus laevis ubiquitin-like modi...   189   1e-46
CR382122_592(CR382122|pid:none) Kluyveromyces lactis strain NRRL...   189   2e-46

 psg: 0.75  gvh: 0.28  alm: 0.27  top: 0.43  tms: 0.07  mit: 0.15  mip: 0.00
 nuc: 0.09  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.02  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: nuclear
	 24.0 %: cytoplasmic
	 20.0 %: endoplasmic reticulum
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHC152 is nuc

>VHC152F.Seq
AGATTTGTCTTCTCAATTTTATTTTTCACCAGAACAAGTTGGTAAAGTTGGTAGAGCAGA
TGCATGTTTCCAAAAAGTTGTAGATTTAAATAATTATGTTAGAATTGATGTACATAATGG
AGAATTAAGTGATGAATTCTTAAAGAAATTCAATGTTGTAGTATTAGCCAATCAACCATT
AGCATTACAACTCAAAGTGAATGAATTTTGTCATGCAAATAAAATTCATTTCATTAGTGT
TGAAACCAGAGGTGTTTTTGGTCAATTATTCAATGATTTTGGTGAACAATTTACAATCAC
CGATACAAATGGTGAGAATCCAAATGCTTATATGATCAGTTCAATCAGTCAAGATAAAGA
AGGTATTGTAACCGTTGTTGAAGAACAAAAATTACAACTTTTAGATGGTGATCTTGTTAC
ATTCAAAGAAGTCAATGGTATGTCAGCTCTTAATGATCTTCCACCACAAAAAATTAAAAC
CATCTCTCCATTAACTTTTTCAATTGGTGATACTACAAATCTTCCACCATATACTTCTGG
TGGTTATGTTACTGAAGTTAAACAACCAAAAGTTGTTGATTTTAANNNNNNNNNN

>VHC152Z.Seq
NNNNNNNNNNAACTCTATTCAATTTGAAAAGGATGATGATACCAATCATCATATCGATTT
CATTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCAATCAGTCCAGCTGATAAACA
TAAGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTAGTTACCACTACTGCCGTAGT
TGCTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATA
TAAGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGC
CCCAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTTTGGGATCGTTTCGATGTTGA
TGGTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATGGTTTGGATAT
TTCAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTCTTCACTGATAAGAAAACTAA
AGAGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAAACCCTCTCAAAGAAACCATT
ACCAAAAGATAAGAAATATTTATTACTGAAATTTGTTGCAAGATAC

>VHC152P.Seq
AGATTTGTCTTCTCAATTTTATTTTTCACCAGAACAAGTTGGTAAAGTTGGTAGAGCAGA
TGCATGTTTCCAAAAAGTTGTAGATTTAAATAATTATGTTAGAATTGATGTACATAATGG
AGAATTAAGTGATGAATTCTTAAAGAAATTCAATGTTGTAGTATTAGCCAATCAACCATT
AGCATTACAACTCAAAGTGAATGAATTTTGTCATGCAAATAAAATTCATTTCATTAGTGT
TGAAACCAGAGGTGTTTTTGGTCAATTATTCAATGATTTTGGTGAACAATTTACAATCAC
CGATACAAATGGTGAGAATCCAAATGCTTATATGATCAGTTCAATCAGTCAAGATAAAGA
AGGTATTGTAACCGTTGTTGAAGAACAAAAATTACAACTTTTAGATGGTGATCTTGTTAC
ATTCAAAGAAGTCAATGGTATGTCAGCTCTTAATGATCTTCCACCACAAAAAATTAAAAC
CATCTCTCCATTAACTTTTTCAATTGGTGATACTACAAATCTTCCACCATATACTTCTGG
TGGTTATGTTACTGAAGTTAAACAACCAAAAGTTGTTGATTTTAA----------AACTC
TATTCAATTTGAAAAGGATGATGATACCAATCATCATATCGATTTCATTACCGCTACCTC
AAATTTAAGAGCTACCAACTATGCAATCAGTCCAGCTGATAAACATAAGACCAAAGGTAT
CGCCGGTAAAATCATTCCAGCTTTAGTTACCACTACTGCCGTAGTTGCTGGTTTCGTTTG
TATCGAATTGATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATATAAGAGCACCTTTAT
GAATTTAGGTATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGCCCCAAAGAACAAAAT
TCGTGAAGGCTGGACTTGGACACTTTGGGATCGTTTCGATGTTGATGGTGATATCACTCT
CAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATGGTTTGGATATTTCAATGCTCTCTTG
TAAAGTTACACTTTTATATGCTCTCTTCACTGATAAGAAAACTAAAGAGGAAAGATTAAA
GATGAAAATCTCACAACTCTATGAAACCCTCTCAAAGAAACCATTACCAAAAGATAAGAA
ATATTTATTACTGAAATTTGTTGCAAGATAC