>VHC144 (VHC144Q) /CSM/VH/VHC1-B/VHC144Q.Seq.d/
ATGTATTGTACTCCAGATGGTTTCAACTTTGAGCAAGGTGATGACCCAATTCAAGATGAC
CCAAATTTATTCGATAATAACGTAGACTCAAGAGCTGAACAGTTCACTCAAGTGGCACTT
GAATATGCAACTCATTATCGTACCAATAATGTACTAATTCCATTTGGTTGTGATTTCGCT
TACCTTAACGCTCAAATGTATTACAAAAACATTGACAAACTAATCGCACATATCAATTCA
AATCCGGATAAATATGGTTTAAATTTGTTATATTCAACTCCATCAATTTATATAGATGCT
GTAAATGATGCCAATCTAGTATGGGAAGTGAAAACTGATGATCTCTTCCCATATGCTGAT
AATGAATTTAGTTATTGGACTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTACGTT
CGTCAAAATAATGCTCTATTACATGTAGTTGAACAAATGTTGGTAACTAGTAGTAATCTT
ATGCCAAGTAGTAGATCTGAACAATTGGTTGATGACATTGTTATAATGAGAGAGGTAATG
GGTATTGCTCAACATCATGATGCCGTATCAGGAACAGAACAACAACATGTTGCAGATGAT
TATGCAGAACGTCTTTCAATTGXXXXXXXXXXCAACCAGTGGTAATTATGTACCTGTGAA
TGCTATTGCNTACATTCAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAG
AAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTAC
TTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTAC
AACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGC
TGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAA
CGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACC
AAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTT
ACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCAC
TTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTT
AACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAA
AAACTATGATCATAAATCATCATCATCAACTAAAGAAGATTCTTCAAAGGTTTTGTTTTT
ACTTTTTCTCCAATGCAAAT

MYCTPDGFNFEQGDDPIQDDPNLFDNNVDSRAEQFTQVALEYATHYRTNNVLIPFGCDFA
YLNAQMYYKNIDKLIAHINSNPDKYGLNLLYSTPSIYIDAVNDANLVWEVKTDDLFPYAD
NEFSYWTGYFVSRPALKGYVRQNNALLHVVEQMLVTSSNLMPSSRSEQLVDDIVIMREVM
GIAQHHDAVSGTEQQHVADDYAERLSI---

---TSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGRGV
GQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQYQG
VYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIFS
TITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKVLFLLFLQCK

Frame A:
MYCTPDGFNFEQGDDPIQDDPNLFDNNVDSRAEQFTQVALEYATHYRTNNVLIPFGCDFA
YLNAQMYYKNIDKLIAHINSNPDKYGLNLLYSTPSIYIDAVNDANLVWEVKTDDLFPYAD
NEFSYWTGYFVSRPALKGYVRQNNALLHVVEQMLVTSSNLMPSSRSEQLVDDIVIMREVM
GIAQHHDAVSGTEQQHVADDYAERLSI---

---qpvvimyl*mllxtfkilinhynsplslievvdvhr*emvnwi**civvl*kmmvvv
lvnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclqplnnlqtigilnik
vfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihklsl*ifqlsf
qqlqsllqlk*i*lvfknyqiyqd*ngkllmvktmiinhhhqlkkilqrfcfyffsnan

Frame B:
civlqmvstlskvmtqfkmtqiysiit*tqelnsslkwhlnmqliivpimy*fhlvvisl
tltlkcitktltn*shisiqirinmv*icyiqlhqfi*ml*mmpi*ygk*klmisshmli
mnlviglvislvvqh*katfvkimlyym*lnkcw*lvvilcqvvdlnnwlmtll**er*w
vllnimmpyqeqnnnmlqmimqnvfql---

---nqw*lctcecycxhsrs*siitihhch**kswmcivkrwsigyddassyfer*wswc
wstne*vnsnryn**idfp*yfklcsislsscctlfitsitpnvynhstifkrlefsisr
clltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstnyhfrsfnylf
nnynhfcn*nefnwcskiikfikikmenc*w*kl*s*iiiin*rrffkgfvftfspmq

Frame C:
vlysrwfql*ar**pnsr*pkfir**rrlks*tvhssgt*icnslsyq*ctnsiwl*frl
p*rsnvlqkh*qtnrtyqfksg*iwfkfvifnsinlyrcck*cqssmgsen**slpic**
*i*lldwlfr*sssierlrssk*csitcs*tnvgn***syak**i*tig**hcynergng
ycsts*crirnrtttccr*lcrtsfn---

---TSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGRGV
GQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQYQG
VYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIFS
TITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKVLFLLFLQCK

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC144 (VHC144Q) /CSM/VH/VHC1-B/VHC144Q.Seq.d/                       2591   0.0
VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1398   0.0
VHP841 (VHP841Q) /CSM/VH/VHP8-B/VHP841Q.Seq.d/                       1398   0.0
VHP767 (VHP767Q) /CSM/VH/VHP7-C/VHP767Q.Seq.d/                       1398   0.0
VHP443 (VHP443Q) /CSM/VH/VHP4-B/VHP443Q.Seq.d/                       1398   0.0
VHO643 (VHO643Q) /CSM/VH/VHO6-B/VHO643Q.Seq.d/                       1398   0.0
VHK686 (VHK686Q) /CSM/VH/VHK6-D/VHK686Q.Seq.d/                       1398   0.0
VHK605 (VHK605Q) /CSM/VH/VHK6-A/VHK605Q.Seq.d/                       1398   0.0
VHJ468 (VHJ468Q) /CSM/VH/VHJ4-C/VHJ468Q.Seq.d/                       1398   0.0
VHI312 (VHI312Q) /CSM/VH/VHI3-A/VHI312Q.Seq.d/                       1398   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1302   0.0    2
CA097818|CA097818.1 SCMCCL6051E03.g CL6 Saccharum officinarum cDNA clone SCMCCL6051E03 5', mRNA sequence.    54   5e-08  3
CD209445|CD209445.1 HS1_42_F06.g1_A012 Heat-shocked seedlings Sorghum bicolor cDNA clone HS1_42_F06_A012 5', mRNA sequence.    54   8e-08  3
CO459584|CO459584.1 MZCCL20022B07.g Maize Endosperm cDNA Library Zea mays cDNA, mRNA sequence.    54   1e-07  3
BF255585|BF255585.2 HVSMEf0007E04f Hordeum vulgare seedling root EST library HVcDNA0007 (Etiolated and unstressed) Hordeum vulgare subsp. vulgare cDNA clone HVSMEf0007E04f, mRNA sequence.    66   3e-06  1
EH757587|EH757587.1 CNSL2408.b1_P01.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSL2408, mRNA sequence.    42   1e-05  2
EH754601|EH754601.1 CNSL13768.b1_P10.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSL13768, mRNA sequence.    42   1e-05  2
EH754563|EH754563.1 CNSL13732.b1_H02.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSL13732, mRNA sequence.    42   1e-05  2
CF036990|CF036990.1 QCG39h06.yg QCG Zea mays cDNA clone QCG39h06, mRNA sequence.    54   1e-05  2
CD440194|CD440194.1 EL01N0551H08.b Endosperm_5 Zea mays cDNA, mRNA sequence.    54   2e-05  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   428   e-118
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   428   e-118
BC091843_1(BC091843|pid:none) Danio rerio zgc:110815, mRNA (cDNA...   198   4e-49
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...   187   1e-45
AE014134_1973(AE014134|pid:none) Drosophila melanogaster chromos...   185   4e-45
AY119107_1(AY119107|pid:none) Drosophila melanogaster RE08556 fu...   180   1e-43
AE014134_1633(AE014134|pid:none) Drosophila melanogaster chromos...   179   2e-43
BC121882_1(BC121882|pid:none) Xenopus tropicalis lysosomal alpha...   177   6e-43
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   174   5e-42
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...   174   5e-42

 psg: 0.75  gvh: 0.18  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for VHC144 is nuc

>VHC144F.Seq
ATGTATTGTACTCCAGATGGTTTCAACTTTGAGCAAGGTGATGACCCAATTCAAGATGAC
CCAAATTTATTCGATAATAACGTAGACTCAAGAGCTGAACAGTTCACTCAAGTGGCACTT
GAATATGCAACTCATTATCGTACCAATAATGTACTAATTCCATTTGGTTGTGATTTCGCT
TACCTTAACGCTCAAATGTATTACAAAAACATTGACAAACTAATCGCACATATCAATTCA
AATCCGGATAAATATGGTTTAAATTTGTTATATTCAACTCCATCAATTTATATAGATGCT
GTAAATGATGCCAATCTAGTATGGGAAGTGAAAACTGATGATCTCTTCCCATATGCTGAT
AATGAATTTAGTTATTGGACTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTACGTT
CGTCAAAATAATGCTCTATTACATGTAGTTGAACAAATGTTGGTAACTAGTAGTAATCTT
ATGCCAAGTAGTAGATCTGAACAATTGGTTGATGACATTGTTATAATGAGAGAGGTAATG
GGTATTGCTCAACATCATGATGCCGTATCAGGAACAGAACAACAACATGTTGCAGATGAT
TATGCAGAACGTCTTTCAATTGNNNNNNNNNN

>VHC144Z.Seq
NNNNNNNNNNCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCNTACATTCAAGAT
CCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTA
AGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGT
GTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCAT
GATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCA
TTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAA
GGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACT
CTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAA
ATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTT
TCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCA
AATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCATCA
TCATCAACTAAAGAAGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAATGCAAAT

>VHC144P.Seq
ATGTATTGTACTCCAGATGGTTTCAACTTTGAGCAAGGTGATGACCCAATTCAAGATGAC
CCAAATTTATTCGATAATAACGTAGACTCAAGAGCTGAACAGTTCACTCAAGTGGCACTT
GAATATGCAACTCATTATCGTACCAATAATGTACTAATTCCATTTGGTTGTGATTTCGCT
TACCTTAACGCTCAAATGTATTACAAAAACATTGACAAACTAATCGCACATATCAATTCA
AATCCGGATAAATATGGTTTAAATTTGTTATATTCAACTCCATCAATTTATATAGATGCT
GTAAATGATGCCAATCTAGTATGGGAAGTGAAAACTGATGATCTCTTCCCATATGCTGAT
AATGAATTTAGTTATTGGACTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTACGTT
CGTCAAAATAATGCTCTATTACATGTAGTTGAACAAATGTTGGTAACTAGTAGTAATCTT
ATGCCAAGTAGTAGATCTGAACAATTGGTTGATGACATTGTTATAATGAGAGAGGTAATG
GGTATTGCTCAACATCATGATGCCGTATCAGGAACAGAACAACAACATGTTGCAGATGAT
TATGCAGAACGTCTTTCAATTG----------CAACCAGTGGTAATTATGTACCTGTGAA
TGCTATTGCNTACATTCAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAG
AAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTAC
TTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTAC
AACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGC
TGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAA
CGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACC
AAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTT
ACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCAC
TTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTT
AACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAA
AAACTATGATCATAAATCATCATCATCAACTAAAGAAGATTCTTCAAAGGTTTTGTTTTT
ACTTTTTCTCCAATGCAAAT