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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHC132
Library VH
(Link to library)
Clone ID VHC132
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15717-1
Original site URL
Representative seq. ID VHC132P
(Link to Original site)
Representative DNA sequence 
>VHC132 (VHC132Q) /CSM/VH/VHC1-B/VHC132Q.Seq.d/
ACAACTTCAAAAATAATATTTTTTTTTATATTATTATATAATTAAAAAAAAAATAATTAT
AAAATGAACTCACTCGTTAAGGGTATTTCCAAAGTTAGATCAACTAGATCTTTTTCAACA
GTATCAATGAGTCCTCTTGAACCAAACAAGAAACTCAACTACGAAGGTATTGATGCTAAA
TTAAAACAATTCANACTCCATCACAACAAACCACTCACCCTTGCTNGXXXXXXXXXXAGG
TCATTTGGATAACATTTCCAACAANATGTTAANTGGNGNTATCAACTTTGAAAACGGTAA
ANCCAACGCTGTTTTCAATCAATTCNCTGGTGAAATCGGTCCAGTNCCAACCGTNGGTTG
TGAATACAAAAAANGTGGNGTCAANTGGATNGTNGTTGGTGATGAAAANTANGGNGAAGG
TTNATTTTGTGAACANGNTGCCCTNGAACCNCGTCATTTCGGTGGTAAANCTATCCTCGN
TAAATCATTTGGTTGNATCCNCGAAACCNATTTCAANAAACAAGGNATTTTACCNTTAAC
CTTTGNTAACCCATTTGATTNCGATAAAATTTTTGGNGACGATTGTANTTCAATCATTGG
TTTCAAANATNTTGCCCCAGGTAAACAANTCNCTTTCATTGTTAAATTTGGTAAACAAGG
TTTTGAATTTGAAANTAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
ttskiifffillyn*KKNNYKMNSLVKGISKVRSTRSFSTVSMSPLEPNKKLNYEGIDAK
LKQFXLHHNKPLTLA---

---GHLDNISNXMLXGXINFENGKXNAVFNQFXGEIGPVPTVGCEYKKXGVXWXVVGDEX
XGEGXFCEXXALEPRHFGGKXILXKSFGXIXETXFXKQGILPLTFXNPFDXDKIFGDDCX
SIIGFKXXAPGKQXXFIVKFGKQGFEFEXK
Translated Amino Acid sequence (All Frames) 
Frame A:
ttskiifffillyn*KKNNYKMNSLVKGISKVRSTRSFSTVSMSPLEPNKKLNYEGIDAK
LKQFXLHHNKPLTLA---

---rsfg*hfqqxvxwxyql*kr*xqrcfqsixw*nrssxnrxl*iqkxwxqxdxxw**k
xxxrxil*txcpxtxsfrw*xypr*iiwlxprnxfqxtrxftxnlx*pi*xr*nfwxrlx
fnhwfqxxcpr*txxfhc*iw*trf*i*x*

Frame B:
qlqk*yfflyyyiikkkiiik*thslrvfpkldqldlfqqyq*vllnqtrnsttkvlmln
*nnsxsittnhspll---

---GHLDNISNXMLXGXINFENGKXNAVFNQFXGEIGPVPTVGCEYKKXGVXWXVVGDEX
XGEGXFCEXXALEPRHFGGKXILXKSFGXIXETXFXKQGILPLTFXNPFDXDKIFGDDCX
SIIGFKXXAPGKQXXFIVKFGKQGFEFEXK

Frame C:
nfknniffyiii*lkkk*l*neltr*gyfqs*in*iffnsiness*tkqetqlrry*c*i
ktixtpsqqtthpcx---

---viwitfptxc*xxxstlktvxptlfsinslvksvqxqpxvvntkxvxsxgxxlvmkx
xxkvxfvnxxpxnxvisvvxlssxnhlvxsxkpisxnkxfyx*plxthlixikflxtivx
qslvsxxlpqvnxslsllnlvnkvlnlkx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC132 (VHC132Q) /CSM/VH/VHC1-B/VHC132Q.Seq.d/                        989   0.0
CHH191 (CHH191Q) /CSM/CH/CHH1-D/CHH191Q.Seq.d/                        634   0.0
VHA855 (VHA855Q) /CSM/VH/VHA8-C/VHA855Q.Seq.d/                        620   e-177
VHK655 (VHK655Q) /CSM/VH/VHK6-C/VHK655Q.Seq.d/                        615   e-175
VHK586 (VHK586Q) /CSM/VH/VHK5-D/VHK586Q.Seq.d/                        615   e-175
VHK566 (VHK566Q) /CSM/VH/VHK5-C/VHK566Q.Seq.d/                        615   e-175
VHK391 (VHK391Q) /CSM/VH/VHK3-D/VHK391Q.Seq.d/                        605   e-172
VHM853 (VHM853Q) /CSM/VH/VHM8-C/VHM853Q.Seq.d/                        597   e-170
VHI739 (VHI739Q) /CSM/VH/VHI7-B/VHI739Q.Seq.d/                        597   e-170
CHR293 (CHR293Q) /CSM/CH/CHR2-D/CHR293Q.Seq.d/                        597   e-170
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY930968|AY930968.1 Rhagoletis electromorpha isolate Relect8 locus P3060 genomic sequence.    42   0.003  2
AY930967|AY930967.1 Rhagoletis electromorpha isolate Relect5 locus P3060 genomic sequence.    42   0.003  2
AC147823|AC147823.1 Xenopus tropicalis clone CH216-127G21, WORKING DRAFT SEQUENCE, 6 unordered pieces.    46   1.3    1
AL423062|AL423062.1 T7 end of clone AZ0AA005H09 of library AZ0AA from strain CBS 712 of Kluyveromyces marxianus.    46   1.3    1
EB100225|EB100225.1 EST62657 Larval Stage 1 Aedes aegypti cDNA clone AENR-LS1-197-A05-U.AB1 5', mRNA sequence.    44   5.1    1
EB095059|EB095059.1 EST57491 Larval Stage 1 Aedes aegypti cDNA clone AEDR-LS1-155-E01-U.AB1 5', mRNA sequence.    44   5.1    1
EB093870|EB093870.1 EST56302 Larval Stage 1 Aedes aegypti cDNA clone AEGR-LS1-168-H08-U.AB1 5', mRNA sequence.    44   5.1    1
EB093014|EB093014.1 EST55446 Larval Stage 1 Aedes aegypti cDNA clone AENR-LS1-144-C12-U.AB1 5', mRNA sequence.    44   5.1    1
EB089074|EB089074.1 EST51506 Larval Stage 1 Aedes aegypti cDNA clone AEGR-LS1-154-D02-U.AB1 5', mRNA sequence.    44   5.1    1
DW996076|DW996076.1 EST48457 Larval Stage 1 Aedes aegypti cDNA clone AEVR-LS1-081-C09-U.AB1 5', mRNA sequence.    44   5.1    1
dna update 2007. 4.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF428141_1(AF428141|pid:none) Gallus gallus aconitase precursor ...   145   8e-34
Z49931_1(Z49931|pid:none) B.taurus mRNA for mitochondrial aconit...   143   4e-33
(Q99KI0) RecName: Full=Aconitate hydratase, mitochondrial;      ...   143   4e-33
DQ468384_1(DQ468384|pid:none) Bos taurus BTA05 5597 genomic sequ...   143   4e-33
BC102642_1(BC102642|pid:none) Bos taurus aconitase 2, mitochondr...   143   4e-33
(P20004) RecName: Full=Aconitate hydratase, mitochondrial;      ...   143   4e-33
BC058049_1(BC058049|pid:none) Danio rerio aconitase 2, mitochond...   143   4e-33
AK144207_1(AK144207|pid:none) Mus musculus kidney CCL-142 RAG cD...   143   4e-33
AK297213_1(AK297213|pid:none) Homo sapiens cDNA FLJ52327 complet...   142   9e-33
AK314845_1(AK314845|pid:none) Homo sapiens cDNA, FLJ95737, highl...   142   9e-33
protein update 2009. 6.27
PSORT 

 psg: 0.75  gvh: 0.44  alm: 0.52  top: 0.53  tms: 0.00  mit: 0.45  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: mitochondrial
	 16.0 %: nuclear
	 12.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHC132 is mit
5' end seq. ID VHC132F
5' end seq. 
>VHC132F.Seq
ACAACTTCAAAAATAATATTTTTTTTTATATTATTATATAATTAAAAAAAAAATAATTAT
AAAATGAACTCACTCGTTAAGGGTATTTCCAAAGTTAGATCAACTAGATCTTTTTCAACA
GTATCAATGAGTCCTCTTGAACCAAACAAGAAACTCAACTACGAAGGTATTGATGCTAAA
TTAAAACAATTCANACTCCATCACAACAAACCACTCACCCTTGCTNGNNNNNNNNNN
Length of 5' end seq. 237
3' end seq. ID VHC132Z
3' end seq. 
>VHC132Z.Seq
NNNNNNNNNNAGGTCATTTGGATAACATTTCCAACAANATGTTAANTGGNGNTATCAACT
TTGAAAACGGTAAANCCAACGCTGTTTTCAATCAATTCNCTGGTGAAATCGGTCCAGTNC
CAACCGTNGGTTGTGAATACAAAAAANGTGGNGTCAANTGGATNGTNGTTGGTGATGAAA
ANTANGGNGAAGGTTNATTTTGTGAACANGNTGCCCTNGAACCNCGTCATTTCGGTGGTA
AANCTATCCTCGNTAAATCATTTGGTTGNATCCNCGAAACCNATTTCAANAAACAAGGNA
TTTTACCNTTAACCTTTGNTAACCCATTTGATTNCGATAAAATTTTTGGNGACGATTGTA
NTTCAATCATTGGTTTCAAANATNTTGCCCCAGGTAAACAANTCNCTTTCATTGTTAAAT
TTGGTAAACAAGGTTTTGAATTTGAAANTAAA
Length of 3' end seq. 452
Connected seq. ID VHC132P
Connected seq. 
>VHC132P.Seq
ACAACTTCAAAAATAATATTTTTTTTTATATTATTATATAATTAAAAAAAAAATAATTAT
AAAATGAACTCACTCGTTAAGGGTATTTCCAAAGTTAGATCAACTAGATCTTTTTCAACA
GTATCAATGAGTCCTCTTGAACCAAACAAGAAACTCAACTACGAAGGTATTGATGCTAAA
TTAAAACAATTCANACTCCATCACAACAAACCACTCACCCTTGCTNG----------AGG
TCATTTGGATAACATTTCCAACAANATGTTAANTGGNGNTATCAACTTTGAAAACGGTAA
ANCCAACGCTGTTTTCAATCAATTCNCTGGTGAAATCGGTCCAGTNCCAACCGTNGGTTG
TGAATACAAAAAANGTGGNGTCAANTGGATNGTNGTTGGTGATGAAAANTANGGNGAAGG
TTNATTTTGTGAACANGNTGCCCTNGAACCNCGTCATTTCGGTGGTAAANCTATCCTCGN
TAAATCATTTGGTTGNATCCNCGAAACCNATTTCAANAAACAAGGNATTTTACCNTTAAC
CTTTGNTAACCCATTTGATTNCGATAAAATTTTTGGNGACGATTGTANTTCAATCATTGG
TTTCAAANATNTTGCCCCAGGTAAACAANTCNCTTTCATTGTTAAATTTGGTAAACAAGG
TTTTGAATTTGAAANTAAA
Length of connected seq. 669
Full length Seq ID -
Full length Seq. -
Length of full length seq. -