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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHC109
Library VH
(Link to library)
Clone ID VHC109
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15687-1
Original site URL
Representative seq. ID VHC109P
(Link to Original site)
Representative DNA sequence 
>VHC109 (VHC109Q) /CSM/VH/VHC1-A/VHC109Q.Seq.d/
AATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTC
AAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGC
AACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAG
AAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAA
TTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAA
TTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAG
AAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAAT
CATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTAT
CAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGA
TCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTG
TCTATTATTCAATGATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATTACAC
AAAATACTGCTXXXXXXXXXXCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGT
CAGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAA
GATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGC
TAGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGA
ATACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACA
TTTAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGAT
CAATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATT
CACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAA
AGATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAAA
GAGAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGCATCACAATCATTTAGCAA
TTAAAAAGATTCACAAATTTCCAACAAGAATA
sequence update 2002.10.25
Translated Amino Acid sequence 
*ktnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIE
EALDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKE
TAESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASI
YQSQKNIHTQFFKNVHLQAVYYSMIDIFSVLINLDSIITQNTA---

---THIGNAMGYIRLVRSGGLHYCSNAIKFVPDLKKIPKFQDLTSKDALSPETIQASTNL
DSIIHNLSNNLSEGTEYFKMLVNVFATEFRNIANQHLKNFYIIVPALASNYIDHMINSKD
KLFKKSKAIGAEALLFTDDGFAIGLAYILKLLDQNKDFDSLHWFDRIQLKCETDQKRMLA
EANAKGIKEASQSFSN*kdsqisnkn
Translated Amino Acid sequence (All Frames) 
Frame A:
nkkrttqfrkwphpirlimvkmknihlmhlkmvhlnwlesnn*lnlinlqlimhnkfgr*
kkh*mkh*mkygi*i*iqfqfiinlmnn*i*lnwlkliikfsik*y*yslqfviklef*k
kqqkvnfihh*qylerlqvnhqkvmyklklvnyyhl*liyqhl*idvih*leillvnlhr
sinhkriyihnslrmfiyklsiiq*liyfqy**i*iv*lhkil---

---hsyw*cygly*igqirwftll**cyqirtrlekdskiprfnlkrcplt*nnss*yqf
gfnhs*sfk*firgy*il*dvgkrfryrvskyr*stfkeflyhrtclglklhrsydq**r
*tl*eiksnrc*sfiih**wlcnwfglylkiirsk*rfrfsslvrsysiem*n*skenvg
*skckryqrgitii*qlkrftnfqqe

Frame B:
ikneqpnlenghilfg**wsk*riyi*ci*rwfi*igwratin*t*sics*lcttnledr
rsir*nik*smgykyrssfnl**ts*tirfn*ig*n*s*nfq*snisilfnl*sn*nfkr
nsrk*ilftinsiwrdyr*iirr*ctn*sw*iitiyd*fisick*mlfin*kyy*sicid
lsitkeytytil*ecsftscllfnd*yifsinkfr*ynytkyc---

---THIGNAMGYIRLVRSGGLHYCSNAIKFVPDLKKIPKFQDLTSKDALSPETIQASTNL
DSIIHNLSNNLSEGTEYFKMLVNVFATEFRNIANQHLKNFYIIVPALASNYIDHMINSKD
KLFKKSKAIGAEALLFTDDGFAIGLAYILKLLDQNKDFDSLHWFDRIQLKCETDQKRMLA
EANAKGIKEASQSFSN*kdsqisnkn

Frame C:
*ktnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIE
EALDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKE
TAESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASI
YQSQKNIHTQFFKNVHLQAVYYSMIDIFSVLINLDSIITQNTA---

---lilvmlwvildwsdpvvytivvmlsnsyqt*krfqnski*pqkmpshlkqfklvpiw
iqsfiifqiiyqrvlntlrcw*tfslqsfeislini*risissylpwpqitsii*sivki
nslrnqkqsvlklyyslmmalqlvwlis*ny*ikikisilfigsivfn*nvklikrecwl
kqmqkvskrhhnhlaikkihkfptri
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC109 (VHC109Q) /CSM/VH/VHC1-A/VHC109Q.Seq.d/                       2502   0.0
VHQ112 (VHQ112Q) /CSM/VH/VHQ1-A/VHQ112Q.Seq.d/                       1330   0.0
VHP507 (VHP507Q) /CSM/VH/VHP5-A/VHP507Q.Seq.d/                       1330   0.0
VHA138 (VHA138Q) /CSM/VH/VHA1-B/VHA138Q.Seq.d/                       1330   0.0
SHK609 (SHK609Q) /CSM/SH/SHK6-A/SHK609Q.Seq.d/                       1330   0.0
SHE452 (SHE452Q) /CSM/SH/SHE4-C/SHE452Q.Seq.d/                       1330   0.0
SHD353 (SHD353Q) /CSM/SH/SHD3-C/SHD353Q.Seq.d/                       1330   0.0
SHC138 (SHC138Q) /CSM/SH/SHC1-B/SHC138Q.Seq.d/                       1330   0.0
SHB243 (SHB243Q) /CSM/SH/SHB2-B/SHB243Q.Seq.d/                       1330   0.0
CHR728 (CHR728Q) /CSM/CH/CHR7-B/CHR728Q.Seq.d/                       1330   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU746157|BU746157.1 CH3#002_C07T7 Canine heart normalized cDNA Library in pBluescript Canis familiaris cDNA clone CH3#002_C07 5', mRNA sequence.    38   8e-04  3
CP000263|CP000263.1 Buchnera aphidicola str. Cc (Cinara cedri), complete genome.    36   0.006  15
BY464171|BY464171.1 Mus musculus 15 days pregnant adult female amnion cDNA, RIKEN full-length enriched library, clone:K630150M24, 3' end partial sequence.    42   0.033  2
AC168241|AC168241.2 Bos taurus clone CH240-197K22, *** SEQUENCING IN PROGRESS ***, 28 unordered pieces.    52   0.040  1
BB822673|BB822673.1 Mus musculus mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830022O04, 3' end partial sequence.    32   0.11   4
DN287505|DN287505.1 1241755 MARC 7BOV Bos taurus cDNA 3', mRNA sequence.    34   0.13   3
CR103667|CR103667.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP142o16.    50   0.16   1
CR044130|CR044130.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP186g09.    50   0.16   1
CR034434|CR034434.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP47e19.    50   0.16   1
CR023465|CR023465.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP421e10.    50   0.16   1
dna update 2007. 4.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54R74) RecName: Full=UPF0681 protein;                               424   e-117
AB028956_1(AB028956|pid:none) Homo sapiens mRNA for KIAA1033 pro...   204   6e-51
BC143373_1(BC143373|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   204   6e-51
BC040936_1(BC040936|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   204   6e-51
BC020314_1(BC020314|pid:none) Mus musculus RIKEN cDNA A230046K03...   199   1e-49
(Q3UMB9) RecName: Full=UPF0681 protein KIAA1033;                      199   1e-49
AK161247_1(AK161247|pid:none) Mus musculus adult male testis cDN...   199   1e-49
AK145000_1(AK145000|pid:none) Mus musculus mammary gland RCB-052...   199   1e-49
BC110850_1(BC110850|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   165   3e-39
CR858798_1(CR858798|pid:none) Pongo abelii mRNA; cDNA DKFZp468B1...   153   1e-35
protein update 2009. 2.24
PSORT 

 psg: 0.79  gvh: 0.25  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHC109 is nuc
5' end seq. ID VHC109F
5' end seq. 
>VHC109F.Seq
AATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTC
AAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGC
AACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAG
AAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAA
TTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAA
TTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAG
AAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAAT
CATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTAT
CAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGA
TCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTG
TCTATTATTCAATGATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATTACAC
AAAATACTGCTNNNNNNNNNN
Length of 5' end seq. 681
3' end seq. ID VHC109Z
3' end seq. 
>VHC109Z.Seq
NNNNNNNNNNCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCAGATCCGGTG
GTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAAT
TCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAATT
TGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACTTTAAGA
TGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAATT
TCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCAATAGTAAAG
ATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTGATGATG
GCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATTTCGATT
CTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAAAGAGAATGTTGG
CTGAAGCAAATGCAAAAGGTATCAAAGAGGCATCACAATCATTTAGCAATTAAAAAGATT
CACAAATTTCCAACAAGAATA
Length of 3' end seq. 621
Connected seq. ID VHC109P
Connected seq. 
>VHC109P.Seq
AATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTC
AAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGC
AACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAG
AAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAA
TTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAA
TTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAG
AAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAAT
CATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTAT
CAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGA
TCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTG
TCTATTATTCAATGATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATTACAC
AAAATACTGCT----------CACTCATATTGGTAATGCTATGGGTTATATTAGATTGGT
CAGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAA
GATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGC
TAGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGA
ATACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACA
TTTAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGAT
CAATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATT
CACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAA
AGATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAAA
GAGAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGCATCACAATCATTTAGCAA
TTAAAAAGATTCACAAATTTCCAACAAGAATA
Length of connected seq. 1282
Full length Seq ID -
Full length Seq. -
Length of full length seq. -