>VHB891 (VHB891Q) /CSM/VH/VHB8-D/VHB891Q.Seq.d/
ATAAATTAATTATTTTTATCTATTTCTTTAAGACAAATTATATAAATCAATTATATAAAT
AGATAATATAAAGAAAAATAGGAATAATAAAATAAATAAAAAAATAAAATAAAAAGATGT
CAGCATTAACACAATCAGGATCAATAGAGAAACCATGTACAATTTTAATCAACTATGATA
AAGGTGATCCACCACAAGTCAATGAATTCAAACAAAACTTCGAACATGGTACAACTGAAC
AAAAGATTGAAACATTAAAAAAGGTTATTCTCTATACAATCAATGGTGAACCAATTCCAC
AATTACTTATGCCAATCATTCTTTATGTTATGCCATCAAATGATCATACCATTAAAAAAC
TATTACTCATCTATTGGGAAGTAATTGAAAAAACTCATCTTGGTAAATTAAAATCCGAAA
TGATTTTAGTATGTAATTCATTATTAAATGATTTAAATCATCCAAATGAATTTGTACGTG
GTAGTACATTAAGATTTTTATGTAAATTAAGAGAAGCAGAAGTTTTAGAACCATTAGTCC
CATCTGTTCGTTCAAACCTTGAGAATAGACATGCATATTGTAGAAGAAATGCAGTTTTAG
CAATCTATAATATCTATAGTCAXXXXXXXXXXCAGTCGATACAGAGGATGATTTAATTAA
AGCAGTTGGTAACACTGGTGAAGCCAATAAAGATCAAAATGAATACTCAAAGATCTCTCA
ATTGTCTGGTTTCTCTGATCCAATTTACGTCGAAGCTTACGTTAGAGTTCATCAATATGA
TATCGTTTTGGATATCTCTGTGTTCAATCAAACCAATGATACCCTTCAAAACGTTACATT
GGAATTGGTCACTTTGGGTGACTTGAAGATTTGCGAACGTGTCCCACCATTTACAATGGC
TCCACGTGAAAAAACTTCAGCCAAAGCTTCAATCAAAGTCTCTTCAACTGATAATGGTGT
AATTATGGGTACAATTGCTTTCGATATCGCTGGTTCCGTCAGTAGTATGTCTGATAAGAA
TTGTGTCATCCTTAATGAACTTCATATTGATGTTATCGATTATATTCTTCCAGCTAATCA
TCAATACACTGATGTTCTTTTCAGAAATCATTGGCTCGAATTTGAATGGGAAAATAAAAT
ACCAGTCAATACAAATATTACCGATTTAGTTAAATACGTTCATCACATTAGTAAAGTCAC
CAACATGGGTATCTTAACACCAGAAGTTCATCTTTCAAATGATACTGGTATCCTCTCTGC
TAATCTTTGTGCTAAAAGTGTTTTCGGTGAAGACGCACTTGCTAATATTTGTATTGAAAA
ACAAGCTGATGGTAAAATTTCTGGTTATATTAGAATTAGAGCTAAAGTTCAATCAATTGC
TGTTACATTAGG

kliifiyffktnyinqlyk*ii*rkigiik*ikk*NKKMSALTQSGSIEKPCTILINYDK
GDPPQVNEFKQNFEHGTTEQKIETLKKVILYTINGEPIPQLLMPIILYVMPSNDHTIKKL
LLIYWEVIEKTHLGKLKSEMILVCNSLLNDLNHPNEFVRGSTLRFLCKLREAEVLEPLVP
SVRSNLENRHAYCRRNAVLAIYNIYS---

---VDTEDDLIKAVGNTGEANKDQNEYSKISQLSGFSDPIYVEAYVRVHQYDIVLDISVF
NQTNDTLQNVTLELVTLGDLKICERVPPFTMAPREKTSAKASIKVSSTDNGVIMGTIAFD
IAGSVSSMSDKNCVILNELHIDVIDYILPANHQYTDVLFRNHWLEFEWENKIPVNTNITD
LVKYVHHISKVTNMGILTPEVHLSNDTGILSANLCAKSVFGEDALANICIEKQADGKISG
YIRIRAKVQSIAVTL

Frame A:
in*lflsislrqii*inyinr*ykek*e**nk*knkikrcqh*hnqdq*rnhvqf*stmi
kvihhksmnsnktsnmvqlnkrlkh*krlfsiqsmvnqfhnylcqsffmlchqmiiplkn
yyssigk*lkklilvn*npk*f*yvihy*mi*iiqmnlyvvvh*dfyvn*ekqkf*nh*s
hlfvqtlridmhiveemqf*qsiisiv---

---qsiqrmi*lkqlvtlvkpikikmntqrslnclvsliqftskltlefinmisfwislc
sikpmipfktlhwnwslwvt*rfanvshhlqwlhvkklqpklqskslqlimv*lwvqlls
islvpsvvclirivsslmnfilmlsiiffqliintlmffseiigsnlngkikyqsiqilp
i*lntfitlvksptwvs*hqkfifqmilvssllifvlkvfsvkthllifvlknklmvkfl
vilelelkfnqlllh*

Frame B:
*inyfylfl*dklyksii*idnikknrnnkinkkik*kdvsintirinretmynfnql**
r*sttsq*iqtklrtwyn*tkd*nikkgyslynqw*tnstityanhslcyaik*syh*kt
ithllgsn*knssw*ikirndfsm*fiik*fkssk*ictw*yikifm*ikrsrsfrtisp
icsfkp*e*tcil*kkcsfsnl*yl*s---

---sryrg*fn*ssw*hw*sq*rsk*ilkdlsivwfl*snlrrslr*sssi*yrfgylcv
qsnq*ypskryigighfg*ledlrtcptiyngst*knfsqsfnqslfn**wcnygyncfr
yrwfrq*yv**elchp**tsy*cyrlysss*ssih*csfqkslari*mgk*ntsqykyyr
fs*irssh**shqhgylntrsssfk*ywyplc*slc*kcfr*rrtc*yly*kts*w*nfw
ly*n*s*ssinccyir

Frame C:
kliifiyffktnyinqlyk*ii*rkigiik*ikk*NKKMSALTQSGSIEKPCTILINYDK
GDPPQVNEFKQNFEHGTTEQKIETLKKVILYTINGEPIPQLLMPIILYVMPSNDHTIKKL
LLIYWEVIEKTHLGKLKSEMILVCNSLLNDLNHPNEFVRGSTLRFLCKLREAEVLEPLVP
SVRSNLENRHAYCRRNAVLAIYNIYS---

---VDTEDDLIKAVGNTGEANKDQNEYSKISQLSGFSDPIYVEAYVRVHQYDIVLDISVF
NQTNDTLQNVTLELVTLGDLKICERVPPFTMAPREKTSAKASIKVSSTDNGVIMGTIAFD
IAGSVSSMSDKNCVILNELHIDVIDYILPANHQYTDVLFRNHWLEFEWENKIPVNTNITD
LVKYVHHISKVTNMGILTPEVHLSNDTGILSANLCAKSVFGEDALANICIEKQADGKISG
YIRIRAKVQSIAVTL

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB891 (VHB891Q) /CSM/VH/VHB8-D/VHB891Q.Seq.d/                       2472   0.0
VHO864 (VHO864Q) /CSM/VH/VHO8-C/VHO864Q.Seq.d/                       1507   0.0
VHC701 (VHC701Q) /CSM/VH/VHC7-A/VHC701Q.Seq.d/                       1497   0.0
AHD247 (AHD247Q) /CSM/AH/AHD2-B/AHD247Q.Seq.d/                       1310   0.0
AHC759 (AHC759Q) /CSM/AH/AHC7-C/AHC759Q.Seq.d/                       1310   0.0
VHP390 (VHP390Q) /CSM/VH/VHP3-D/VHP390Q.Seq.d/                       1308   0.0
AHQ161 (AHQ161Q) /CSM/AH/AHQ1-C/AHQ161Q.Seq.d/                       1308   0.0
AHF471 (AHF471Q) /CSM/AH/AHF4-C/AHF471Q.Seq.d/                       1308   0.0
VHA685 (VHA685Q) /CSM/VH/VHA6-D/VHA685Q.Seq.d/                       1306   0.0
SHF684 (SHF684Q) /CSM/SH/SHF6-D/SHF684Q.Seq.d/                       1306   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U62960|U62960.1 Dictyostelium discoideum CopB (copB) mRNA, partial cds.  1507   0.0    1
AF247143|AF247143.1 Dictyostelium discoideum beta-COP protein mRNA, complete cds.  1507   0.0    1
AB261041|AB261041.1 Entamoeba histolytica gene for beta1-COP, complete cds.    52   2e-09  4
DV977319|DV977319.1 GQ00610.B3.1_L03 GQ006: Cambium and phloem from mature trees Picea glauca cDNA clone GenomeQuebec_Id:GQ00610_L03 5', mRNA sequence.    46   6e-06  3
DR569740|DR569740.1 WS02625.BR_J06 WS-PP-N-A-12 Picea glauca cDNA clone WS02625_J06 5, mRNA sequence.    46   6e-06  3
EC825794|EC825794.1 SME00007153 esmbsro2 Sawyeria marylandensis cDNA, mRNA sequence.    44   7e-05  2
DY890578|DY890578.1 CeleSEQ9632 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ9632, mRNA sequence.    56   8e-05  2
DY892831|DY892831.1 CeleSEQ11848 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ11848, mRNA sequence.    56   8e-05  2
DY892567|DY892567.1 CeleSEQ11131 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ11131, mRNA sequence.    56   8e-05  2
DY894270|DY894270.1 CeleSEQ13804 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ13804, mRNA sequence.    56   9e-05  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U62960_1(U62960|pid:none) Dictyostelium discoideum CopB (copB) m...   501   e-140
(Q23924) RecName: Full=Coatomer subunit beta; AltName: Full=Beta...   501   e-140
BT045694_1(BT045694|pid:none) Salmo salar clone ssal-rgf-528-379...   273   1e-71
BT070792_1(BT070792|pid:none) Picea sitchensis clone WS02750_E10...   272   2e-71
(P23514) RecName: Full=Coatomer subunit beta; AltName: Full=Beta...   271   3e-71
(Q9JIF7) RecName: Full=Coatomer subunit beta; AltName: Full=Beta...   271   5e-71
BC081657_1(BC081657|pid:none) Danio rerio coatomer protein compl...   270   7e-71
AY294010_1(AY294010|pid:none) Danio rerio coatomer protein compl...   270   1e-70
(Q5R922) RecName: Full=Coatomer subunit beta; AltName: Full=Beta...   268   3e-70
BC073438_1(BC073438|pid:none) Xenopus laevis MGC80934 protein, m...   267   8e-70

 psg: 0.83  gvh: 0.33  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: plasma membrane
	  4.0 %: mitochondrial

>> prediction for VHB891 is cyt

>VHB891F.Seq
ATAAATTAATTATTTTTATCTATTTCTTTAAGACAAATTATATAAATCAATTATATAAAT
AGATAATATAAAGAAAAATAGGAATAATAAAATAAATAAAAAAATAAAATAAAAAGATGT
CAGCATTAACACAATCAGGATCAATAGAGAAACCATGTACAATTTTAATCAACTATGATA
AAGGTGATCCACCACAAGTCAATGAATTCAAACAAAACTTCGAACATGGTACAACTGAAC
AAAAGATTGAAACATTAAAAAAGGTTATTCTCTATACAATCAATGGTGAACCAATTCCAC
AATTACTTATGCCAATCATTCTTTATGTTATGCCATCAAATGATCATACCATTAAAAAAC
TATTACTCATCTATTGGGAAGTAATTGAAAAAACTCATCTTGGTAAATTAAAATCCGAAA
TGATTTTAGTATGTAATTCATTATTAAATGATTTAAATCATCCAAATGAATTTGTACGTG
GTAGTACATTAAGATTTTTATGTAAATTAAGAGAAGCAGAAGTTTTAGAACCATTAGTCC
CATCTGTTCGTTCAAACCTTGAGAATAGACATGCATATTGTAGAAGAAATGCAGTTTTAG
CAATCTATAATATCTATAGTCANNNNNNNNNN

>VHB891Z.Seq
NNNNNNNNNNCAGTCGATACAGAGGATGATTTAATTAAAGCAGTTGGTAACACTGGTGAA
GCCAATAAAGATCAAAATGAATACTCAAAGATCTCTCAATTGTCTGGTTTCTCTGATCCA
ATTTACGTCGAAGCTTACGTTAGAGTTCATCAATATGATATCGTTTTGGATATCTCTGTG
TTCAATCAAACCAATGATACCCTTCAAAACGTTACATTGGAATTGGTCACTTTGGGTGAC
TTGAAGATTTGCGAACGTGTCCCACCATTTACAATGGCTCCACGTGAAAAAACTTCAGCC
AAAGCTTCAATCAAAGTCTCTTCAACTGATAATGGTGTAATTATGGGTACAATTGCTTTC
GATATCGCTGGTTCCGTCAGTAGTATGTCTGATAAGAATTGTGTCATCCTTAATGAACTT
CATATTGATGTTATCGATTATATTCTTCCAGCTAATCATCAATACACTGATGTTCTTTTC
AGAAATCATTGGCTCGAATTTGAATGGGAAAATAAAATACCAGTCAATACAAATATTACC
GATTTAGTTAAATACGTTCATCACATTAGTAAAGTCACCAACATGGGTATCTTAACACCA
GAAGTTCATCTTTCAAATGATACTGGTATCCTCTCTGCTAATCTTTGTGCTAAAAGTGTT
TTCGGTGAAGACGCACTTGCTAATATTTGTATTGAAAAACAAGCTGATGGTAAAATTTCT
GGTTATATTAGAATTAGAGCTAAAGTTCAATCAATTGCTGTTACATTAGG

>VHB891P.Seq
ATAAATTAATTATTTTTATCTATTTCTTTAAGACAAATTATATAAATCAATTATATAAAT
AGATAATATAAAGAAAAATAGGAATAATAAAATAAATAAAAAAATAAAATAAAAAGATGT
CAGCATTAACACAATCAGGATCAATAGAGAAACCATGTACAATTTTAATCAACTATGATA
AAGGTGATCCACCACAAGTCAATGAATTCAAACAAAACTTCGAACATGGTACAACTGAAC
AAAAGATTGAAACATTAAAAAAGGTTATTCTCTATACAATCAATGGTGAACCAATTCCAC
AATTACTTATGCCAATCATTCTTTATGTTATGCCATCAAATGATCATACCATTAAAAAAC
TATTACTCATCTATTGGGAAGTAATTGAAAAAACTCATCTTGGTAAATTAAAATCCGAAA
TGATTTTAGTATGTAATTCATTATTAAATGATTTAAATCATCCAAATGAATTTGTACGTG
GTAGTACATTAAGATTTTTATGTAAATTAAGAGAAGCAGAAGTTTTAGAACCATTAGTCC
CATCTGTTCGTTCAAACCTTGAGAATAGACATGCATATTGTAGAAGAAATGCAGTTTTAG
CAATCTATAATATCTATAGTCA----------CAGTCGATACAGAGGATGATTTAATTAA
AGCAGTTGGTAACACTGGTGAAGCCAATAAAGATCAAAATGAATACTCAAAGATCTCTCA
ATTGTCTGGTTTCTCTGATCCAATTTACGTCGAAGCTTACGTTAGAGTTCATCAATATGA
TATCGTTTTGGATATCTCTGTGTTCAATCAAACCAATGATACCCTTCAAAACGTTACATT
GGAATTGGTCACTTTGGGTGACTTGAAGATTTGCGAACGTGTCCCACCATTTACAATGGC
TCCACGTGAAAAAACTTCAGCCAAAGCTTCAATCAAAGTCTCTTCAACTGATAATGGTGT
AATTATGGGTACAATTGCTTTCGATATCGCTGGTTCCGTCAGTAGTATGTCTGATAAGAA
TTGTGTCATCCTTAATGAACTTCATATTGATGTTATCGATTATATTCTTCCAGCTAATCA
TCAATACACTGATGTTCTTTTCAGAAATCATTGGCTCGAATTTGAATGGGAAAATAAAAT
ACCAGTCAATACAAATATTACCGATTTAGTTAAATACGTTCATCACATTAGTAAAGTCAC
CAACATGGGTATCTTAACACCAGAAGTTCATCTTTCAAATGATACTGGTATCCTCTCTGC
TAATCTTTGTGCTAAAAGTGTTTTCGGTGAAGACGCACTTGCTAATATTTGTATTGAAAA
ACAAGCTGATGGTAAAATTTCTGGTTATATTAGAATTAGAGCTAAAGTTCAATCAATTGC
TGTTACATTAGG