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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB869
Library VH
(Link to library)
Clone ID VHB869
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11129-1
Original site URL
Representative seq. ID VHB869P
(Link to Original site)
Representative DNA sequence 
>VHB869 (VHB869Q) /CSM/VH/VHB8-C/VHB869Q.Seq.d/
GTTAATAGCAAAAGAAAAATGGCAACAAAAAATACATCTCCTTATGAGATTGAATATGCA
AAGAGTGATAGATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCAGTTCGT
ATTGGTTATAAAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCATCATTTA
AAATGTAAATGTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATACCTTCGT
TGGGAAGACCAATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGATCCAAAA
TCAGCATCAGAGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAAGATAGT
ATTGCCGATAACTTGAAAGGACCAGCAATTAAAAACATCATTCAATATAATAAAGGTTAT
GTTGATAAGGTATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTAAAAGGT
CGTCCAGGTAGATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATCGAATAT
CAATGTAAAGGTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCAATTGAA
AGATXXXXXXXXXXAAGCCAAGATGCAATTGGTTGAAACTCTTATCGATGTTGATATTGC
CGCTAATCTTAAGAAGCAAACTGAATCACTCGAGGGTAACATGATTGAAAATCAATATAT
TACACTCAAAACCAAACTCACTCCACTTGATAAAGATAGTGTAGTTTATAAAACCTTGGT
AGATTATGTTGCCAATAGTCAAGATAAACAATTTAGAACCAATATCTGTGTTCAAGATAT
TCTTTCACTTGAGAGAGAGAATGAAAGTGAACGTTTCACTCCTTGGGCAAAAGATAAGAA
TAGATTACTCTTATGGCATGGTAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAGGG
ATTAAGAATTGCTCCACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATTTA
TTTTGCTGATTGTATTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCAAC
TGCACTTATGTTATTATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACATGATAC
CTATATGGAAGAGGCACCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGCTCC
TCACAAAGATGGTAATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGTAA
AATTTCAAAGACTGGTCTCTCAACTTCATGTACTCACAATGAATTCATTGTCTATAAAAT
TGAACAAGTCAGAATCAAATACATCTTAAGAGTTTACAAGGAAAATAAAATAAAATAAAA
TTTTTTAAAATT
sequence update 2002.10.25
Translated Amino Acid sequence 
VNSKRKMATKNTSPYEIEYAKSDRSTCSTCQRGINKEAVRIGYKTKSKHFDGMDVSWHHL
KCKCPQVPSFTDLIHWEYLRWEDQLSIKTTYFSSEKYDPKSASEVQREKYLKALWEVKDS
IADNLKGPAIKNIIQYNKGYVDKVSPVHLLHTLSDWMLKGRPGRCPTCKNFDILFNGIEY
QCKGWISGFTKCDWKGDSIER---

---AKMQLVETLIDVDIAANLKKQTESLEGNMIENQYITLKTKLTPLDKDSVVYKTLVDY
VANSQDKQFRTNICVQDILSLERENESERFTPWAKDKNRLLLWHGSRLTNFVSIVSQGLR
IAPPSAPKTGYRFGKGIYFADCISKSFSYCFTSSDCPTALMLLCEVSLGDMNELKHDTYM
EEAPHPFHSTKALGMAAPHKDGNHPLSDSDGLVVPLGKISKTGLSTSCTHNEFIVYKIEQ
VRIKYILRVYKENKIK*nflk
Translated Amino Acid sequence (All Frames) 
Frame A:
VNSKRKMATKNTSPYEIEYAKSDRSTCSTCQRGINKEAVRIGYKTKSKHFDGMDVSWHHL
KCKCPQVPSFTDLIHWEYLRWEDQLSIKTTYFSSEKYDPKSASEVQREKYLKALWEVKDS
IADNLKGPAIKNIIQYNKGYVDKVSPVHLLHTLSDWMLKGRPGRCPTCKNFDILFNGIEY
QCKGWISGFTKCDWKGDSIER---

---kprcnwlkllsmlilplilrsklnhsrvt*lkinilhskpnslhlikiv*fikpw*i
mlpivkinnlepisvfkiffhlrermkvnvsllgqkiridysygmvvg*lisyrlsrrd*
ellhhllqklvtdlvkvfillivlvnhsaivshqvivqlhlcyyvkfhlvt*mn*nmipi
wkrhhihsivqkl*vwllltkmviilflilmv**yh*vkfqrlvsqlhvltmnslsikln
ksesnts*eftrkik*nkif*n

Frame B:
liakekwqqkihllmrlnmqrvidqhvqpvkevlikkqfvlvikqnqntlmewmyhgii*
nvnvhkyhhlqi*ftgntfvgktnyqlkqlifhlksmiqnqhqrykerni*rhygklkiv
lpit*kdqqlktsfniikvmliryhqfiyfilsligc*kvvqvdvqlvrisiyfsmvsni
nvkvgfqvsqnvigkviqlkd---

---sqdaig*nsyrc*ycr*s*ean*itrg*hd*ksiyytqnqthst**r*csl*nlgrl
ccq*sr*ti*nqylcsrysft*ere*k*tfhslgkr*e*itlmaw**vn*fridclagik
ncsticsknwlqiw*rylfc*ly**iiqllfhik*lsnctyvim*sftw*he*iet*yly
grgttsip*yksfrygcssqrw*sssf*f*wfsstir*nfkdwslnfmysq*ihcl*n*t
sqnqihlkslqgk*nkikffki

Frame C:
**qkkngnkkyisl*d*icke**inmfnlskry**rsssywl*nkiktl*wngcimasfk
m*mststiiyrfnslgipslgrpiin*nnlffi*kv*skisirgtkreifkgimgs*r*y
cr*lertsn*khhsi**rlc**gitssftsysl*ldvkrssr*msnl*efrytfqwyris
m*rldfrfhkm*ler*fn*k---

---AKMQLVETLIDVDIAANLKKQTESLEGNMIENQYITLKTKLTPLDKDSVVYKTLVDY
VANSQDKQFRTNICVQDILSLERENESERFTPWAKDKNRLLLWHGSRLTNFVSIVSQGLR
IAPPSAPKTGYRFGKGIYFADCISKSFSYCFTSSDCPTALMLLCEVSLGDMNELKHDTYM
EEAPHPFHSTKALGMAAPHKDGNHPLSDSDGLVVPLGKISKTGLSTSCTHNEFIVYKIEQ
VRIKYILRVYKENKIK*nflk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB869 (VHB869Q) /CSM/VH/VHB8-C/VHB869Q.Seq.d/                       2637   0.0
AHB712 (AHB712Q) /CSM/AH/AHB7-A/AHB712Q.Seq.d/                       1463   0.0
SHG306 (SHG306Q) /CSM/SH/SHG3-A/SHG306Q.Seq.d/                       1431   0.0
SHB222 (SHB222Q) /CSM/SH/SHB2-A/SHB222Q.Seq.d/                       1419   0.0
VHB303 (VHB303Q) /CSM/VH/VHB3-A/VHB303Q.Seq.d/                       1413   0.0
AHM188 (AHM188Q) /CSM/AH/AHM1-D/AHM188Q.Seq.d/                       1407   0.0
AHI282 (AHI282Q) /CSM/AH/AHI2-D/AHI282Q.Seq.d/                       1405   0.0
AHR444 (AHR444Q) /CSM/AH/AHR4-B/AHR444Q.Seq.d/                       1402   0.0
AHH466 (AHH466Q) /CSM/AH/AHH4-C/AHH466Q.Seq.d/                       1388   0.0
CHP243 (CHP243Q) /CSM/CH/CHP2-B/CHP243Q.Seq.d/                       1378   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ534948|AJ534948.1 Dictyostelium discoideum mRNA for NAD(+) ADP-ribosyltransferase-1A (adprt-1A gene).  1469   0.0    1
AJ520099|AJ520099.1 Dictyostelium discoideum mRNA for (ADP-ribosyl)transferase (adprt2  gene).    62   4e-14  3
DY229813|DY229813.1 ID0AAK8YC07CM1 ApHL3SD Acyrthosiphon pisum cDNA clone ID0AAK8YC07 5', mRNA sequence.    60   2e-04  1
AM642769|AM642769.1 Entamoeba dispar GSS, clone dispar98f12.p1k.    54   3e-04  2
EB501254|EB501254.1 1099435318866 12-DrosW-norm-1P5Kb Drosophila willistoni cDNA clone 1061018595867, mRNA sequence.    56   0.003  1
EB500928|EB500928.1 1099435305233 12-DrosW-norm-1P5Kb Drosophila willistoni cDNA clone 1061018598938, mRNA sequence.    56   0.003  1
EB493727|EB493727.1 1099341581900 12-DrosW-norm-1P5Kb Drosophila willistoni cDNA clone 1061018595867, mRNA sequence.    56   0.003  1
EB489445|EB489445.1 1099341224857 12-DrosW-norm-1P5Kb Drosophila willistoni cDNA clone 1061018544138, mRNA sequence.    56   0.003  1
EB455916|EB455916.1 1099341378745 12-DrosW-std-1P6Kb Drosophila willistoni cDNA clone 1061018613759, mRNA sequence.    56   0.003  1
AC025807|AC025807.2 Homo sapiens clone RP11-657G2, WORKING DRAFT SEQUENCE, 15 unordered pieces.    50   0.17   1
dna update 2007. 4.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

PDBF(1A26) MOL_ID: 1; MOLECULE: POLY (ADP-RIBOSE) POLYMERASE; CH...   204   8e-51
(P26446) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   204   8e-51
X65497_1(X65497|pid:none) R.norvegicus mRNA for poly(ADP-ribose)...   202   2e-50
(P27008) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   202   2e-50
AK303340_1(AK303340|pid:none) Homo sapiens cDNA FLJ53442 complet...   201   5e-50
(Q9R152) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   201   7e-50
AK312339_1(AK312339|pid:none) Homo sapiens cDNA, FLJ92658, highl...   200   9e-50
J03473_1(J03473|pid:none) Human poly(ADP-ribose) synthetase mRNA...   200   9e-50
AB384394_1(AB384394|pid:none) Synthetic construct DNA, clone: pF...   200   9e-50
M17081_1(M17081|pid:none) Human placental poly(ADP-ribose) polym...   200   9e-50
protein update 2009. 6.27
PSORT 

 psg: 0.58  gvh: 0.23  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.39  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 24.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHB869 is nuc
5' end seq. ID VHB869F
5' end seq. 
>VHB869F.Seq
GTTAATAGCAAAAGAAAAATGGCAACAAAAAATACATCTCCTTATGAGATTGAATATGCA
AAGAGTGATAGATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCAGTTCGT
ATTGGTTATAAAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCATCATTTA
AAATGTAAATGTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATACCTTCGT
TGGGAAGACCAATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGATCCAAAA
TCAGCATCAGAGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAAGATAGT
ATTGCCGATAACTTGAAAGGACCAGCAATTAAAAACATCATTCAATATAATAAAGGTTAT
GTTGATAAGGTATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTAAAAGGT
CGTCCAGGTAGATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATCGAATAT
CAATGTAAAGGTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCAATTGAA
AGATNNNNNNNNNN
Length of 5' end seq. 614
3' end seq. ID VHB869Z
3' end seq. 
>VHB869Z.Seq
NNNNNNNNNNAAGCCAAGATGCAATTGGTTGAAACTCTTATCGATGTTGATATTGCCGCT
AATCTTAAGAAGCAAACTGAATCACTCGAGGGTAACATGATTGAAAATCAATATATTACA
CTCAAAACCAAACTCACTCCACTTGATAAAGATAGTGTAGTTTATAAAACCTTGGTAGAT
TATGTTGCCAATAGTCAAGATAAACAATTTAGAACCAATATCTGTGTTCAAGATATTCTT
TCACTTGAGAGAGAGAATGAAAGTGAACGTTTCACTCCTTGGGCAAAAGATAAGAATAGA
TTACTCTTATGGCATGGTAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAGGGATTA
AGAATTGCTCCACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATTTATTTT
GCTGATTGTATTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCAACTGCA
CTTATGTTATTATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACATGATACCTAT
ATGGAAGAGGCACCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGCTCCTCAC
AAAGATGGTAATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGTAAAATT
TCAAAGACTGGTCTCTCAACTTCATGTACTCACAATGAATTCATTGTCTATAAAATTGAA
CAAGTCAGAATCAAATACATCTTAAGAGTTTACAAGGAAAATAAAATAAAATAAAATTTT
TTAAAATT
Length of 3' end seq. 788
Connected seq. ID VHB869P
Connected seq. 
>VHB869P.Seq
GTTAATAGCAAAAGAAAAATGGCAACAAAAAATACATCTCCTTATGAGATTGAATATGCA
AAGAGTGATAGATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCAGTTCGT
ATTGGTTATAAAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCATCATTTA
AAATGTAAATGTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATACCTTCGT
TGGGAAGACCAATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGATCCAAAA
TCAGCATCAGAGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAAGATAGT
ATTGCCGATAACTTGAAAGGACCAGCAATTAAAAACATCATTCAATATAATAAAGGTTAT
GTTGATAAGGTATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTAAAAGGT
CGTCCAGGTAGATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATCGAATAT
CAATGTAAAGGTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCAATTGAA
AGAT----------AAGCCAAGATGCAATTGGTTGAAACTCTTATCGATGTTGATATTGC
CGCTAATCTTAAGAAGCAAACTGAATCACTCGAGGGTAACATGATTGAAAATCAATATAT
TACACTCAAAACCAAACTCACTCCACTTGATAAAGATAGTGTAGTTTATAAAACCTTGGT
AGATTATGTTGCCAATAGTCAAGATAAACAATTTAGAACCAATATCTGTGTTCAAGATAT
TCTTTCACTTGAGAGAGAGAATGAAAGTGAACGTTTCACTCCTTGGGCAAAAGATAAGAA
TAGATTACTCTTATGGCATGGTAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAGGG
ATTAAGAATTGCTCCACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATTTA
TTTTGCTGATTGTATTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCAAC
TGCACTTATGTTATTATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACATGATAC
CTATATGGAAGAGGCACCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGCTCC
TCACAAAGATGGTAATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGTAA
AATTTCAAAGACTGGTCTCTCAACTTCATGTACTCACAATGAATTCATTGTCTATAAAAT
TGAACAAGTCAGAATCAAATACATCTTAAGAGTTTACAAGGAAAATAAAATAAAATAAAA
TTTTTTAAAATT
Length of connected seq. 1382
Full length Seq ID -
Full length Seq. -
Length of full length seq. -